LOCUS       NZ_OW967009             8328 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli isolate 624 plasmid P6, complete sequence.
ACCESSION   NZ_OW967009
VERSION     NZ_OW967009.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065689
            Assembly: GCF_905330065.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330065.2-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:21:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,485
            CDSs (total)                      :: 5,369
            Genes (coding)                    :: 5,137
            CDSs (with protein)               :: 5,137
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 91 of 232
            Pseudo Genes (incomplete)         :: 162 of 232
            Pseudo Genes (internal stop)      :: 40 of 232
            Pseudo Genes (multiple problems)  :: 51 of 232
            Pseudo Genes (short protein)      :: 1 of 232
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW967009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="624"
                     /isolation_source="Hospital General de Alicante (Alicante)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P6"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            join(8319..8328,1..314)
                     /locus_tag="LQ179_RS27225"
     CDS             join(8319..8328,1..314)
                     /locus_tag="LQ179_RS27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024132211.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            523..>960
                     /locus_tag="LQ179_RS27230"
                     /pseudo=""
     CDS             523..>960
                     /locus_tag="LQ179_RS27230"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            992..>1360
                     /locus_tag="LQ179_RS27235"
                     /pseudo=""
     CDS             992..>1360
                     /locus_tag="LQ179_RS27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /name="MbeB family mobilization protein"
     gene            complement(1510..2370)
                     /locus_tag="LQ179_RS27240"
                     /old_locus_tag="AI2834V1_5296"
     CDS             complement(1510..2370)
                     /locus_tag="LQ179_RS27240"
                     /old_locus_tag="AI2834V1_5296"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor-resistant broad-spectrum class A
                     beta-lactamase TEM-30"
                     /protein_id="WP_021561477.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSSGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="inhibitor-resistant broad-spectrum class A
                     beta-lactamase TEM-30"
     gene            complement(2553..3110)
                     /locus_tag="LQ179_RS27245"
                     /old_locus_tag="AI2834V1_5297"
     CDS             complement(2553..3110)
                     /locus_tag="LQ179_RS27245"
                     /old_locus_tag="AI2834V1_5297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            3274..6279
                     /locus_tag="LQ179_RS27250"
                     /old_locus_tag="AI2834V1_5298"
     CDS             3274..6279
                     /locus_tag="LQ179_RS27250"
                     /old_locus_tag="AI2834V1_5298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143759.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            6514..6747
                     /locus_tag="LQ179_RS27255"
                     /old_locus_tag="AI2834V1_5299"
     CDS             6514..6747
                     /locus_tag="LQ179_RS27255"
                     /old_locus_tag="AI2834V1_5299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(8064..8255)
                     /locus_tag="LQ179_RS27260"
                     /old_locus_tag="AI2834V1_5301"
     CDS             complement(8064..8255)
                     /locus_tag="LQ179_RS27260"
                     /old_locus_tag="AI2834V1_5301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     ARG             complement(1514..2370)
                     /name="blaTEM-30"
                     /gene="blaTEM-30"
                     /gene_name="inhibitor-resistant broad-spectrum class A
                     beta-lactamase TEM-30"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7900..7983
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.059"
                     /coverage="95.0"
                     /evalue="8.83e-18"
     Typing          305..1362
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.884"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6316..6830
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.746"
                     /coverage="100.0"
                     /evalue="4.15e-71"
ORIGIN
        1 tacgggttac cgatgaggag cacgcccggt tactggaacg ctgcgagggt aaacgactgg
       61 cggaatggat gcggcgggtc tgtctggggg agccggttgc acgcacagga aaactgccga
      121 cactatcgcc gccattgctg cggcatctgg ctgccatcgg gaacaacctg aaccagacag
      181 cccggaaggt gaacagcggc cagtggtctt cgattgaccg ggttcatgtg gtggccgcac
      241 tgatggccat cgagggtgaa ttgcgccagt tgcggcaggc tgtcagggaa cagggggtac
      301 gggatgatag ttaaatttca tgccaggggg aaaggcggtg gaagtggccc ggtcgattac
      361 ctgctggggc gggagcgtaa ccgcgaaggc gcaacggtgc tccggggtaa cccggaagaa
      421 gtgcgggaac tcattgattc cacgccattc tcgaagaaat acacttcagg cgttctgtcg
      481 ttcgcggaga aggagttacc gccgggtgaa cgcgaaagag tgatgacgag ctttgagcgt
      541 gttcttatgc ccggtctcga aaaagaccag tacagcattc tctgggtgga acaccaggat
      601 aaggggcggc tcgaactgaa ttttgtcatc ccgaacatgg agctacagag cggtaagcgc
      661 ctccagccgt actacgaccg cgcggacagg ccgaggataa atgcctggca gacgctggtt
      721 aatcatcatt acgggctgcg tgacccgaac gcaccggaga acagacggac gcttgtcacg
      781 ccaaataatc tgccaaaagc gaaacaggag gccgcagaag cgattacacg gggtcttgag
      841 gctctttacc atgccggagc aataaaaacg cgtcaggatg tcacagaggc gcttacagcg
      901 gcaggttttg aggtggtgag gacaaccaaa agcagcatca gtattgccga cccggagggg
      961 gggcggaatc tcaggctaaa gggggcgctg tatgagcaat cttttacaaa tgggaacaga
     1021 cttcgagaag aaactgaaag agcgagccgc ttctaccgag aacatgctga acagcgagtt
     1081 caggaagctc gaagagtctg tagacaaggc attgagctta aacaggcaga aaatcagaga
     1141 cgctatcagc gaacatacaa catccgtgaa aaagcaactg gatacgctga gcaccaccgt
     1201 gagcatgcag ttcagcacca cagaggccga actgtcccgg cagcagaaaa aactgctctg
     1261 gcgggtgatc aaagggcgga tactgtttcc ggcgctgacc gcgctcagcg tcacgggcgg
     1321 cattttcctg gggtgctggg ggctgatgga gtggcaggaa agggggtctg acgctcagtg
     1381 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1441 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1501 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1561 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1621 atctggcccc agtgctgcaa tgataccgct agacccacgc tcaccggctc cagatttatc
     1681 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1741 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1801 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     1861 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1921 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1981 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2041 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2101 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcaccacata gcagaacttt
     2161 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2221 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2281 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2341 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2401 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2461 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     2521 tatcatgaca ttaacttata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
     2581 ttataaaccg tggagcgggc aatactgagc tgatgagcaa tttccgttgc accagtgccc
     2641 ttctgatgaa gcgtcagcac gacgttcctg tccacggtac gcctgcggcc aaatttgatt
     2701 cctttcagct ttgcttcctg tcggccctca ttcgtgcgct ctaggatcct ccggcgttca
     2761 gcctgtgcca cagccgacag gatggtgacc accatttgcc ccatatcacc gtcggtactg
     2821 atcccgtcat caatgaaccg gactgccacg ccctgagcgt caaattcctt tatcagttgg
     2881 atcatatcgg cagtgtcgcg gccaagacgg tcgagcttct taaccagaat gacatcacct
     2941 tcctccacct tcatcctcag caaatccagc ccttcccgat ctgttgaact gccggatgcc
     3001 ttatcggtaa atatacggtt tgctttcaca cctgcgtctt tgagtgctct gacctgaaga
     3061 tcaagagact gctgactggt tgagacccga gcgtaaccaa aaagtcgcat aaaaatgtac
     3121 cttaaatcga atatcggaca actcatgtct attattacaa atttacgatt taatagacat
     3181 attaatgtaa cagttttacg atgtccgata atttataaca tttcgtacgg ttgcaaaaat
     3241 gttactaaat gcccgtcagg cagggaggcc gatatgcccg ttgactttct gaccactgag
     3301 cagactgaaa gctatggcag attcaccggt gaaccggatg agcttcagct ggcacgatat
     3361 tttcaccttg atgaagcaga caaggaattt atcggaaaaa gcagaggtga tcacaaccgt
     3421 ctgggcattg ccctgcaaat tggatgtgtc cgttttctgg gcaccttcct caccgatatg
     3481 aatcatattc cttccggcgt ccggcatttt accgccagac agctcgggat tcgtgatatc
     3541 accgttcttg cagaatacgg tcagagggaa aatacccgcc gtgagcatgc agcgctgata
     3601 cgtcagcact atcagtatcg tgaatttgcc tggccctgga catttcgcct tacccgtctt
     3661 ttatataccc ggagctggat aagcaacgaa cgtcctggcc tgcttttcga tctggcgaca
     3721 gggtggctta tgcaacatcg tattattctc cccggagcca ctacgctgac ccggttgatt
     3781 tcagaggtaa gggaaaaggc gacgttgcgc ctgtggaaca aactggcact gataccgtca
     3841 gccgaacagc gttcacagct ggagatgctg ctggggccaa ctgattgcag ccgcctgtct
     3901 ttactggaat cactgaaaaa gggccctgtg accatcagtg gtccggcgtt taatgaagca
     3961 attgaacgct ggaaaactct gaacgatttt ggcctgcatg ctgaaaacct gagtacactc
     4021 ccggctgtgc gcctgaaaaa tctcgcacgt tatgctggta tgacttcggt gttcaatatt
     4081 gccaggatgt caccgcagaa aaggatggcg gttctggttg cctttgtcct tgcatgggaa
     4141 acgctggcgc tggatgatgc attggacgtt ctggacgcca tgctggccgt tatcatccgt
     4201 gacgccagaa agattgggca gaaaaaacgg ctccgctcgc tgaaggatct ggataaatct
     4261 gcattggcgc tcgccagcgc atgttcgtac ctgctgaaag aagaaacacc ggacgaatcg
     4321 attcgtgctg aggtgttcag ctacatccca aggcaaaagc tggctgaaat catcacgctt
     4381 gtccgtgaaa ttgcccggcc ctcagacgat aattttcatg aagaaatggt ggagcagtac
     4441 gggcgcgttc gtcgtttcct gccccatctg ctgaataccg ttaaattttc atccgcacct
     4501 gccggggtta ccactctgaa tgcctgtgac tacctcagcc gggagttcag ctcacggcgg
     4561 cagttttttg acgacgcacc aacggaaatt atcagtcggt catggaaacg gctggtgatt
     4621 aacaaggaaa aacatatcac ccgcagggga tacacgctct gctttctcag taaactgcag
     4681 gatagtctga ggcggaggga tgtctacgtt accggcagta accggtgggg agatcctcgt
     4741 gcaagattac tacagggtgc tgactggcag gcaaaccgga ttaaggttta tcgttctttg
     4801 gggcacccga cagacccgca ggaagcaata aaatctctgg gtcatcagct tgatagtcgt
     4861 tacagacagg ttgctgcacg tctttgcgaa aatgaggctg tcgaactcga tgtttctggc
     4921 ccgaagcccc ggttgacaat ttctcccctc gccagtcttg atgagccgga cagtctgaaa
     4981 cgactgagca aaatgatcag tgatctactc cctccggtgg atttaacgga gttgctgctc
     5041 gaaattaacg cccataccgg atttgctgat gagtttttcc atgctagtga agccagtgcc
     5101 agagttgatg atctgcccgt cagcatcagc gccgtgctga tggctgaagc ctgcaatatc
     5161 ggtctggaac cactgatcag atcaaatgtt cctgcactga cccgacaccg gctgaactgg
     5221 acaaaagcga actatctgcg ggctgaaact atcaccagcg ctaatgccag actggttgat
     5281 tttcaggcaa cgctgccact ggcacagata tggggtggag gagaagtggc atctgcagat
     5341 ggaatgcgct ttgttacgcc agtcagaaca atcaatgccg gaccgaaccg caaatacttt
     5401 ggtaataaca gagggatcac ctggtacaac tttgtgtccg atcagtattc cggctttcat
     5461 ggcatcgtta taccggggac gctgagggac tctatctttg tgctggaagg tcttctggaa
     5521 caggagaccg ggctgaatcc aaccgaaatt atgaccgata cagcaggtgc cagcgaactt
     5581 gtctttggcc ttttctggct gctgggatac cagttttctc cacgcctggc tgatgccggt
     5641 gcttcggttt tctggcgaat ggaccatgat gccgactatg gcgtgctgaa tgatattgcc
     5701 agagggcaat cagatccccg aaaaatagtc cttcagtggg acgaaatgat ccggaccgct
     5761 ggctccctga agctgggcaa agtacaggct tcagtgctgg tccgttcatt gctgaaaagt
     5821 gaacgtcctt ccggactgac tcaggcaatc attgaagtgg ggcgcatcaa caaaacgctg
     5881 tatctgctta attatattga tgatgaagat taccgccggc gcattctgac ccagcttaat
     5941 cggggagaaa gtcgccatgc cgttgccaga gccatctgtc acggtcaaaa aggtgagata
     6001 agaaaacgat ataccgacgg tcaggaagat caactgggcg cactggggct ggtcactaac
     6061 gccgtcgtgt tatggaacac tatttatatg caggcagccc tggatcatct ccgggcgcag
     6121 ggtgaaacac tgaatgatga agatatcgca cgcctctccc cgctttgcca cggacatatc
     6181 aatatgctcg gccattattc cttcacgctg gcagaactgg tgaccaaagg acatctgaga
     6241 ccattaaaag aggcgtcaga ggcagaaaac gttgcttaac gtgagttttc gttccactga
     6301 gcgtcagacc ccgaaagcaa gattgcgaaa aatatcctga ctatcagaga gcaggaaaac
     6361 acgctggcga aactcgaggc aaaaacctgg ggggtgacgt tcgtcaatgg cgagaacggg
     6421 aaatttctgg tgttgccgga cggggtgaaa ggggaaaaca cctggacggt gggcgacaag
     6481 aacgccgtga ggctggtcag ggagtaaacg agtatgacgg aactggaagg gcatttgctg
     6541 aacgccttag agcatttgca acaggactat atgcggcggc tgaacgagtg ggagagcgcc
     6601 ttcgcggaat tgcagaagat gcacgaggtt acgcaacgga acaacgcgat actgaacgag
     6661 cgcgtcgtga tattgagtca gcaggtgcag agattagccg ggcaagtgga tcgcttgagt
     6721 cggttattta tcgcgaacaa cagctaaggg atgaacagga gcgtgcccgg tacagacatg
     6781 aggtacagga acgcgcggca cgggttcgga gtgggccatc aatggagatg tgaccgggga
     6841 agtgacgaaa tcttgccatt ttgaccagtt cgacggctgg ggcgagcgaa acaaatcgtg
     6901 tgccgtattt gtgcatagct atgcaacatc ctgaaaacgc atttcacgcg tttttgggga
     6961 ttcagccagg ggaaaacatg gtttttttac tttttgagcg taaagacgtg aaaaatctcg
     7021 ttattgcact tgcgatcttt cgtcggcggg cgtagaacga aaacagctcg ccaggaaggc
     7081 gagctgtttc gggttcattg aggtctctga cctcctcaat gttcctgtgg aaactgttga
     7141 gatccacaac agaaaccagt tcattcagtt tgcatgagcc ttctgtattc ttcaagaatt
     7201 tttcaacagc acagtctccc aggaaccccg aaaaaaacct gtttttgcag atacttgcgt
     7261 atttgcgaga atcgtttgcg gttgggggag ctgtgacgac ggcgaccgca cgcgtgagag
     7321 ttccgctacc ggcgcagaga gtttcatcga cagcggacac ctgatgagtt gccacaaaaa
     7381 ccgtatgtag cgcggctaga ttggttgagt tagaccttgg cggcagttcc cggtatgtgg
     7441 tggcgtgacg ggtgagatgg tgcggcgaag acgcatacgt cacgcaggcg cgggttcttc
     7501 cactgacgcg ccccgtgatc gatggccttg gctccgtccg gcatatcgag agcggcaaac
     7561 tacccgtccc ttcactggga ggggtgagtt gttgccgttc cctccaacct ttcaccatcc
     7621 ggcatatcgc acgatcccat gtgcaccgca actcctgata actccagtgc aaaaaaaaaa
     7681 aacggatctg catcacaacg agagtgatcc caccgctcgc cgcagcccca gcgaccgaac
     7741 gtagtgagcg agtgggcgag gaagcggaag agtcacagat gcaagcattg tgcacttacg
     7801 cgcctgtgcc agtgtccgcg tgtgtgctgt ttatcgggtg cgctgttttt acatctgtgc
     7861 ggctttttac tcctgacatg gtgcacttcc catccatgtc tgcaccgatg caaaatgaga
     7921 taagccagta tacacatcgc tatcgctctg tgaactgggt cagggctgcg ccccgacacc
     7981 cgcactgacg cgccctggcg ggcttgtctg ctcccggcat ccgctcacag gcaagctgtg
     8041 aacgtctccg ggagctgcat gtttcagttg ctttcaccgt catcgccgaa gcgtgcgagg
     8101 caactgcggt agagttcgtc ggcgtggtcg tgaagtgatt cgcagatgtc tgcctgttcg
     8161 tccgcgtcca gatcgttgag tttttccagc agggttagcg tctggcttct gataaagcga
     8221 gccatgttaa gggctgtttt ttcctgtttg gtcatggtct taaggctcct tgtatggggg
     8281 atttgttagg gggaatgata ccgatgaaac gcgagaagat gctcacga
//
