LOCUS       NZ_OW849531             3576 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Citrobacter freundii isolate 22 plasmid P4, complete sequence.
ACCESSION   NZ_OW849531
VERSION     NZ_OW849531.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7962742
            Assembly: GCF_905232515.2
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:13:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,287
            CDSs (with protein)               :: 5,287
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 32 of 122
            Pseudo Genes (incomplete)         :: 81 of 122
            Pseudo Genes (internal stop)      :: 25 of 122
            Pseudo Genes (multiple problems)  :: 16 of 122
            Pseudo Genes (short protein)      :: 1 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW849531.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /isolate="22"
                     /isolation_source="Hospital Universitario Central de
                     Asturias (Oviedo)"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="P4"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            join(3033..3576,1..395)
                     /locus_tag="LQ181_RS27620"
     CDS             join(3033..3576,1..395)
                     /locus_tag="LQ181_RS27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013185833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200002678.1"
                     /translation="MSVLDFLSSGGMASIGGDLSEEEQKHTNEIVKLFAKKLREYARTL
                     QIPASNIKINKCSFLFVAKDDFSYHFVFADKPGGGNNLVYRNLSEYRQLELQSIVHQVR
                     IDVGDVVWAFSCPVSLSGEELDTYINKLSIEYIQSEIQSSKDNNDGRISEEAMNVPEIA
                     SGLEKFRKDYPKGKKTAFIIMQFGSTAAHVKLVDAIKKELSKHNIIALRADDKEYMDDL
                     FSNIKTYMYGCDFGIAIFERITEDNFNPNVSLEVGYMMGMGKQVLLLKDKTLSSLHTDL
                     AGKLYKPFDVFDIESSLSTHIGKWLSDKGFL"
                     /name="hypothetical protein"
     gene            complement(705..836)
                     /locus_tag="LQ181_RS27705"
     CDS             complement(705..836)
                     /locus_tag="LQ181_RS27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072052181.1"
                     /translation="MHEPPVQSNRCALSGNYRLESNPKRHDKTPLAAATGNGMYKIN"
                     /name="replication initiation protein"
     gene            1254..1442
                     /locus_tag="LQ181_RS27625"
                     /old_locus_tag="AI2661V1_5391"
     CDS             1254..1442
                     /locus_tag="LQ181_RS27625"
                     /old_locus_tag="AI2661V1_5391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044703047.1"
                     /translation="MTKQQKTALNMAKFIQAQSLFLLGNLNEQDLDTEAGLCEKLHEDA
                     ELLFRTLDIRLDDFQED"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1445..1876
                     /locus_tag="LQ181_RS27630"
                     /old_locus_tag="AI2661V1_5392"
     CDS             1445..1876
                     /locus_tag="LQ181_RS27630"
                     /old_locus_tag="AI2661V1_5392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044703046.1"
                     /translation="MARVSISEAARLVSVSRPTIYKLLKSGELSYTSVVKHGKPVKTID
                     TAELIRLFGALDVVSGDKSDTVKFDGVTTSVNSDFLHSLQHQVALLQAENTGLKDAVNA
                     RDEHISSLRQAMQLLEHKQSTSDPQNVPAKSWWQFWKSH"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(729..1073)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.509"
                     /coverage="80.0"
                     /evalue="2.99e-149"
ORIGIN
        1 tcatcatgca gtttggtagc acggcagcgc acgtaaagct tgttgatgca ataaagaaag
       61 agttgtctaa acacaacata atagctttaa gggctgatga taaagagtat atggatgatc
      121 tcttttcaaa tatcaaaaca tatatgtatg ggtgcgattt cggtattgct atttttgaga
      181 ggattacaga agataatttt aaccctaatg tttcattgga agttggatat atgatgggaa
      241 tggggaagca ggttttactt ttaaaggata agactctaag ttcattacat acagacttag
      301 caggtaaact ttataagcca tttgatgttt tcgatattga gtcatccctc agcactcata
      361 ttgggaaatg gctatcagac aagggatttt tatagtttcc ggaacaagta ccgtatgtgc
      421 ggtacttgtt tgacatcgtt agttatcgaa tggggttatg cgaaaatcat ggtcaaattt
      481 gagacttgac gcgtttgatt gatgagatct tcttgagatc cttcgtctct cgtcgtagtc
      541 tcttgttcca gaaacgaaaa aaccgactgg ggagtcggtt ttttcgaggg ttcagcaagt
      601 tggggaactt ctgaaccgtg gtaacagggt gtacaagacc gctgaaaccg taatctgtcc
      661 tttcagttta gccgtagttt agctcggact tcaagaactc tcattcagtt aatcttgtac
      721 atcccattac cagtggccgc tgccagtggc gttttgtcgt gtcttttcgg gttggactca
      781 agacgatagt taccggataa ggcgcagcgg ttggactgaa cggggggttc gtgcatacag
      841 tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagagaaccg
      901 cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
      961 ggagccacca gggggaaacg cctggtatct ttaaagtcct gtcgggtttc gccaccactg
     1021 atttgagcgt caaattttgt gatgcttgtc aggggggcga agcctatgga aaaacgccct
     1081 ggtcgggcat ggctttatcg ttcttgttct ggcttttcgt tacgcttacc gtctacgtta
     1141 tttgtctctt aggaagatgc gtccgctcgc cgcagataag tgaccgagcg tagcgagcga
     1201 acggtcgagg aagcggaaaa gaaccggata tcatttggag cactgacact gtgatgacga
     1261 aacaacagaa aacggcactg aatatggcga aattcattca ggcgcaatcc ctgttcttgc
     1321 tgggtaatct caatgaacag gatctcgata ctgaagccgg cttgtgcgaa aaattgcatg
     1381 aggatgcgga actgcttttt cgtactctgg atattcgtct ggatgatttt caagaggatt
     1441 agtaatggct cgcgtatcca ttagtgaagc tgcacgtctt gtcagtgtca gtcgcccgac
     1501 tatttacaag ctgcttaaat ctggtgagct tagctacacc tccgttgtaa agcatggaaa
     1561 acccgttaaa accattgata cagctgagct tattcgtctt tttggtgctc ttgatgttgt
     1621 cagtggtgat aaaagtgaca ctgtaaaatt tgacggtgtc actacgtcgg ttaacagtga
     1681 ttttttacat tctttacagc atcaagtggc gcttttacag gcggagaata ccgggcttaa
     1741 agatgcagtg aatgcacggg atgagcatat aagttcactt cgtcaggcca tgcaacttct
     1801 ggagcataaa cagagtactt ctgacccgca gaatgttcct gcaaagtcct ggtggcagtt
     1861 ctggaaatcc cattgagtca ttatcaatca tgcattccgg tcatagcgtt cggccagcca
     1921 ccaggctatc accggatatg gatgtttata ctttctcatc tgaccgcttt acccgcatgt
     1981 ttgatttctg atggctgata aaggccaaaa aatcgtgtca caccccacgc agtatacacg
     2041 gctaatgccg gaagcctttg ctgacaaggc ttccgggggt ataccacccc ttgtatgaaa
     2101 tggtataccc gcatctgttt ttatgcggtt aaagccagtt ctggcaagac ctgtaggggg
     2161 tataccaggt ggtaactggc ttggtatacc tgaaaagctg ctctgagggc ttcagtgcca
     2221 cacaggataa gggattgcac ttggtatacc ctttttctgt ttgtgtggtg cgatatcagt
     2281 atctctccct gcgcggtgtt atagataaaa gacctgtcat ggatgcgccc cgacaccctg
     2341 ccagcgcctc acgaccccgc aataaattgc gtgcccgttc accgctttct ttagccagtc
     2401 tgtgccagac tgttatctga atggcttact agtgtggagg taaaaaggtg aaagaatggt
     2461 ttgcgaccct tatcgatgac ctgaaatttc tgggggcgga gtttaagaga gagttttcgg
     2521 atctggagat caggccgatg acggctcagg agaaccgacg gagacatgat gagatgcacc
     2581 gttcttccgt ggccgggaag ttagcacggg gcgctttgca aatcagggac gggaaactga
     2641 ccccctatgg tatgggacgg aacaaatagg cttcatcaat cccggattcc cgggctagta
     2701 aaccgggaaa aatctccctt ttagcggctg ttttccgtca aatcacgcac catgcagccg
     2761 ggagcatagt tattcaccct ctcaggattc atctgtggcg ttctgagcgt tttcccccgg
     2821 gtaaactgta caaaacgacg cactcacccc aaaagcgctc agagtgcgtc tgagcgatgc
     2881 gcgtaatgag tgcttatgtt aaatagatgg cagtagactc aaggtgggtt gtatcattat
     2941 caagtgaata ggtgatgtta ccattaagta gtggttaggt tttgggatgt ccgggccaag
     3001 cgttctttag aattggaaaa aggaaattaa ctatgtctgt tttggatttt ttatcttccg
     3061 gtgggatggc tagtattggt ggagatctgt ctgaagaaga acaaaaacat actaatgaaa
     3121 tagttaaatt atttgccaaa aaattgagag agtatgctag gacattacaa attccagcat
     3181 ccaatatcaa aataaataaa tgttcatttt tgtttgtcgc aaaagacgat ttttcttatc
     3241 atttcgtgtt tgctgataaa cctggtggcg gtaataattt agtatataga aacttatctg
     3301 aatatagaca gcttgaactt caaagtatcg tccatcaggt tcggattgat gttggtgatg
     3361 ttgtttgggc gttctcttgc ccagtctctc tctccgggga agaacttgat acatatatta
     3421 ataaactctc tattgaatat attcagtctg aaattcaaag tagtaaagat aacaatgatg
     3481 gtagaatttc agaagaggct atgaatgtcc ctgaaatagc ttctggcctt gaaaaattca
     3541 gaaaagacta tccaaaaggg aaaaaaacag cattta
//
