LOCUS       NZ_OW849495             3696 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Serratia marcescens isolate 0 plasmid P4, complete sequence.
ACCESSION   NZ_OW849495
VERSION     NZ_OW849495.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7962735
            Assembly: GCF_905232465.2
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/02/2023 08:00:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,620
            CDSs (total)                      :: 5,490
            Genes (coding)                    :: 5,399
            CDSs (with protein)               :: 5,399
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 18
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 32 of 92
            Pseudo Genes (incomplete)         :: 62 of 92
            Pseudo Genes (internal stop)      :: 13 of 92
            Pseudo Genes (multiple problems)  :: 12 of 92
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW849495.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /isolate="0"
                     /isolation_source="Complejo Asistencial Universitario de
                     Salamanca (Salamanca)"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="P4"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(3593..3696,1..28))
                     /locus_tag="LQ146_RS28055"
     CDS             complement(join(3593..3696,1..28))
                     /locus_tag="LQ146_RS28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071605494.1"
                     /translation="MHEPPVQSDHCTLSGNYRLESNPERHDKAPLAAAVGRGVQEIN"
                     /name="replication initiation protein"
     gene            704..1060
                     /locus_tag="LQ146_RS28060"
                     /old_locus_tag="AI2617V1_5360"
     CDS             704..1060
                     /locus_tag="LQ146_RS28060"
                     /old_locus_tag="AI2617V1_5360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_038873324.1"
                     /translation="MAWHSVSQAQALTGKSRRTLYRDMAAGRVSWRNNADGRRELETTE
                     LLRVYGELQPDGTDARHTLSQPVGTEDQAVLLAEIRALRQEVADLKAAVLRLEHHPTPA
                     EPAIVKRWWQRWRR"
                     /name="hypothetical protein"
     gene            1801..2319
                     /locus_tag="LQ146_RS28065"
                     /old_locus_tag="AI2617V1_5361"
     CDS             1801..2319
                     /locus_tag="LQ146_RS28065"
                     /old_locus_tag="AI2617V1_5361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013318635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_038873321.1"
                     /translation="MQKVTDLYPPEIASHNLHNHFAGNEVMLELIKKLNKTSLCTFAAL
                     CDGNVVTTSGYNIMADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQVAFYLD
                     ESDLARLKSEPEKMMKECERNLNSQKRSNAQKEMIRLYKEFGESGVLELLRNVSGANDS
                     PPSDEKKAS"
                     /name="hypothetical protein"
     gene            complement(2530..2946)
                     /locus_tag="LQ146_RS28070"
                     /old_locus_tag="AI2617V1_5362"
     CDS             complement(2530..2946)
                     /locus_tag="LQ146_RS28070"
                     /old_locus_tag="AI2617V1_5362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_038873320.1"
                     /translation="MYIPVVIHKDADTDYGVTVPDIPGCYSAGVTVDEALQNAKEAILF
                     HIEGLIEDGVYHEFKFSDIEALKTYDEYHDGMFSLVDVDLSALTDEATRFNVSWPKYVL
                     TILDGSLKETHETRSGYLAKLVLQDLRRNRSFQD"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            complement(3035..3223)
                     /locus_tag="LQ146_RS28075"
     CDS             complement(3035..3223)
                     /locus_tag="LQ146_RS28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889005.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_071605287.1"
                     /translation="MSSSEDIIRRIQLDGWEKVAQKGSHVQFKHPTKKGRVTIPHPNKD
                     LPKGTVKSILKQAGIAE"
                     /name="type II toxin-antitoxin system HicA family toxin"
     Typing          complement(3..265)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.591"
                     /coverage="80.0"
                     /evalue="2.41e-120"
ORIGIN
        1 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcccgg
       61 aaccgagtgt caggcgtgga atgagataaa cgcggccata acagcggaat gacatcggta
      121 aaccgaaagg caggaacagg agagcgcacg agggagccat cagggggaaa cgcctggtat
      181 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatatt gtgatgcttg
      241 tcaggggggc ggagcctatg gaaaaacagc ccgctgggct gtggcctttt cccgtctttc
      301 tgcatgctct tactccgtca tccttccctt tttatcccca ccgctcgccg cagtgttgac
      361 tagcgtagtc tgtcaacgcg aggaagcgga agtgcatttg actgatatgt cgcacggtca
      421 tcgcggggct tttgttgacg atgcaagata ggcaaagtgt tcccgtttga cgtcattgca
      481 tgccttcatc cggtgacacg ttgcctaccc tgccggtggc tctcgtgcgg gacaggatat
      541 gttttgtagc accgccgtgg gcgacgcgtc caaaacgagg gtgacggaca ggaaatgtgt
      601 tgtcattacc gccggtgttc ctggctttga caacacgcag gtcagggcga agccccgaca
      661 ccccgaatct cccctatact gtgccaaaaa aaaggaggtc tctatggcat ggcacagtgt
      721 gtcgcaggca caggcattga ccggaaaaag tcggcggacg ctctaccgtg atatggcggc
      781 aggccgcgta tcatggcgca ataatgccga tggcaggcgg gagttagaaa ctacggaatt
      841 gctgcgcgtg tacggcgaac tgcagcctga tggcacggat gcgcggcaca cgctgtcaca
      901 gcccgttggc acagaagatc aggcggtatt actcgccgaa atacgcgcat tacggcagga
      961 ggtcgccgat ttgaaagcgg cggtcttgcg tctggagcac catcctaccc cggcggagcc
     1021 ggcgatcgtc aaacggtggt ggcagcgctg gcgccgttga tccccactgc cgcgccgccg
     1081 gcttggccaa cgcttcacgg ccgcgcaata aattgcccgc ccgttcaccg tttctcacat
     1141 tggcagaacg gcaaaaacac gcgattcggt cgcgtttctc tctgaacctg gtctctgcgt
     1201 gctgggttgc ataatcattc atgccgccaa acggcctgta cgccattttg cgggcttttt
     1261 ttgcggagaa gtgagggggt tttactgctt tggttgttca agcgcacata gggcgtctga
     1321 ggcggtgcgg gaaacggatg ttatggtaaa ttgtgaaatt agctgagatt tttttgtcaa
     1381 ctttttgctg tatgtttaaa catgagttct acgctgacag tactgttgct aatttcatct
     1441 gccagactgg taattccctt gtaaatcaat cgcgagagat cagtaaaaaa gctatgtgtg
     1501 tgcttatgga atagggtaga ataagaaatt ctgatgatcg tgaggttgtc agtgaaaaac
     1561 gaggcagcta tcagatcttc ggcctgagaa actaaggatt ctgatagctg ccggtgactc
     1621 ggttcgtgaa aactgaatct acctgatcaa caaacgcccg attagggcgt ttgtcaagtg
     1681 gtgctgcatt atgtagtaac ttggtaccga cttgccaggt atttttcaca gtttttgatg
     1741 cgcgaaggct catacggaca ccgtcacctg ttatttattc tctgtaaaca ggtgctataa
     1801 atgcaaaaag taaccgattt gtatcctcct gaaattgcta gtcataatct tcataatcac
     1861 ttcgctggca acgaagtgat gctggagtta atcaagaaat taaataaaac cagtttgtgt
     1921 acttttgcag ctctctgtga tggtaacgtg gtaaccactt ccggctataa cattatggcc
     1981 gatctgtgtg ttaaccgtgc gtcagctgtg gctcattctc ttaaacaaaa atatcttcca
     2041 gtcaccgcta gaacgatatc gactaaggct gatgttggtg gtgcagtaaa acaggttgct
     2101 ttctatcttg acgagagcga tcttgcaaga ctgaagtctg aacctgaaaa gatgatgaaa
     2161 gagtgcgaac gtaacctgaa tagccagaag cgctccaatg ctcagaagga aatgattagg
     2221 ctatacaaag agtttggtga gtcaggtgtt ttggaacttt tacgtaatgt ttctggtgct
     2281 aatgattcac cgccgtccga tgaaaagaaa gcaagttgaa tgaatgaata aaaaaaggct
     2341 agatttctct agcctttttt cttacttact accagcggag tcttttctgt tgcttctcac
     2401 atgagctcta acagttatta cttcaccact ggataaagtc ttttggtatg acgcaatcct
     2461 attgaatttt ctacctttac tgtcttttgc catgtctgtt cctcctatga gttctatcct
     2521 tgattcacat taatcctgga atgatctatt tctacgcaag tcttgaagca ctaattttgc
     2581 taagtaacct gaacgagtct catgagtttc ttttaatgaa ccatctaaaa tggttaatac
     2641 gtattttggc cagctaacat taaatcttgt cgcttcatct gttaatgcgc taagatctac
     2701 gtcaaccaac gaaaacatac catcatgata ttcatcataa gtttttaagg cttctatatc
     2761 gctgaattta aattcatgat atacgccatc ctcaattaat ccttctatat gaaaaagaat
     2821 cgcctcttta gcattttgca atgcttcatc tacggttaca cctgctgagt aacatccagg
     2881 aatatcagga acagttacgc cataatccgt atcggcatcc ttatggatca ctaccggaat
     2941 gtacatggtt aactcctaat ctctctcatt actcattaaa cgtagtaagt cattctgaac
     3001 tctaccggga aggcaaagcc ttcccggtga ggaatcattc agctatcccg gcctgcttaa
     3061 ggatactttt tacagttccc ttaggcaggt ctttgttcgg gtgaggaatg gttacgcgac
     3121 cctttttagt aggatgttta aactgcacat gactaccttt ttgggccact ttttcccatc
     3181 catcgagctg aattcttcgg attatgtctt cagaagaact catgctacgt accttatggt
     3241 tgtgctctgc ttttgcggag ctgggttcct ttcgtctcca tcaaaagcgg aaactttcga
     3301 caataataca cacgattaca cacggtgtca atctctgtgt gtaatcgtgt gtattcaagc
     3361 ggtaaggaaa gttgctttgt ttctgcttga ttgaatgatt ttcttgagat ctatctgtgc
     3421 tgtcggtaat cttcctttga aacgaaaaaa ccgcctgggg aggcggtttt ttcgagggtt
     3481 cagtaagttg gggaacttct gaaccgtggt aacagggtgt acaagaccgc tgaaaccaaa
     3541 ttctgtcctt tcagtgtagc cgcagttggg ttgccacttc aagaactctc attcagttaa
     3601 tctcttgtac acctctacca acggctgctg ccagtggcgc tttgtcgtgt ctttccgggt
     3661 tggactcaag tcgatagtta ccggataagg tgcagt
//
