LOCUS       NZ_OW849213             3810 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii isolate 18 plasmid P5, complete sequence.
ACCESSION   NZ_OW849213
VERSION     NZ_OW849213.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7962748
            Assembly: GCF_905232605.2
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 16:07:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,482
            CDSs (total)                      :: 5,354
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 33 of 116
            Pseudo Genes (incomplete)         :: 82 of 116
            Pseudo Genes (internal stop)      :: 23 of 116
            Pseudo Genes (multiple problems)  :: 20 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW849213.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /isolate="18"
                     /isolation_source="Hospital Universitario Central de
                     Asturias (Oviedo)"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="P5"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            join(3575..3810,1..88)
                     /locus_tag="LQ122_RS27340"
     CDS             join(3575..3810,1..88)
                     /locus_tag="LQ122_RS27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            297..>731
                     /locus_tag="LQ122_RS27345"
                     /pseudo=""
     CDS             297..>731
                     /locus_tag="LQ122_RS27345"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            766..1257
                     /locus_tag="LQ122_RS27350"
     CDS             766..1257
                     /locus_tag="LQ122_RS27350"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1264..1479
                     /locus_tag="LQ122_RS27355"
     CDS             1264..1479
                     /locus_tag="LQ122_RS27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_046275712.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWQSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2080..2421
                     /locus_tag="LQ122_RS27360"
                     /old_locus_tag="AI2668V1_5336"
     CDS             2080..2421
                     /locus_tag="LQ122_RS27360"
                     /old_locus_tag="AI2668V1_5336"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_074184457.1"
                     /translation="MAKPPSATPPDTPSSCDVSALPGIIQNFGLINFNVSCNNGELTVT
                     GTDQNGKHIRVNSYQGNGFTEGTMSTFQPGGKEARRLEAVRLRESGLTQQEIANRLGVS
                     QKTISNDLK"
                     /name="helix-turn-helix domain-containing protein"
     Typing          3290..3462
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.977"
                     /coverage="100.0"
                     /evalue="4.31e-74"
     Typing          complement(2819..3365)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.636"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          79..1571
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3576..3808
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.59"
                     /coverage="99.0"
                     /evalue="2.64e-43"
ORIGIN
        1 gattgtggcg gcgctgatgg ccatcgatgc cggccttgag cggctgcggc acgccgttct
       61 tgagaaagga acggacgatg atcgttaaat ttcatccgcg cggccgcggc ggtggcgccg
      121 ggccggtgga ctatctgctg ggtaaagacc ggcagcgtga aggcgccacc gtcctgcagg
      181 ggaagccgga agaagtccgg gagctgattg acgcgtcgcc ttacgttaag aaatacacct
      241 ccggcgttct gtcgtttgcg gaggccgatc tgcctcccgg ccagcgggaa aaactgatgg
      301 ccagcttcga gcgggttctg atgcccggac tcgataaaga tcagtacagc attctgtggg
      361 tggagcacgc ggacaagggg cgtctggagc tgaactttct gatccccaat accgagctgc
      421 tgaccggcag gcggctccag ccttactacg accgcgccga ccggccgcgc atcgatgcct
      481 ggcagactat cgtgaacggc agactggggc tgcacgaccc gaacgcgccg gaaaaccgcc
      541 gggcgctggt cacgccgtcc gggctgccga aaacgacgca ggaggccgca gaagccatta
      601 cccggggact gctgaccctt gcttcgtccg gggagctgaa aagccgtcag gatgtcactg
      661 aggcgctgga acggtcaggt tttgaggtgg tgcgcaccac gaaaagcagt atcagcattg
      721 ccgacccgga cggggggcga aacatccgac tgaagggagc catctatgag cagtctttta
      781 acgctggcga aggacttaga gcagcaatcg aaagcgcagc agcagagtac cggcgagatg
      841 ctgaaagccg cattcagcga gcacgagcag tctgtcagag cggaactgag cgcaagcgcg
      901 aggagaatca gcgacgccat cctcgcccac gagcagagta tgagcgaggc gatggagaaa
      961 aaccggcgga gcgtactgcg gactgccggg cggacatggc tcaccatcct gatggtgtca
     1021 gcgctgctga tcggcacgag cgggagcatt ctgtggtggc aggggcagca gataaccgac
     1081 aattacacgc atcttcgtca gcaggaagat accctggcga aaatgaccgc ccggacgtgg
     1141 ggagtgcgct atcaggagag cagcgacggc cggaggtttc ttatcctgcc gccggggatg
     1201 caaaccgaag ccatccctta cgacgggacg acatggatcc gcctgaaaca ggagtagcgg
     1261 aaaatgacag agctggaaaa gcagttgctg agcgcattag agcagctaca gcaggactac
     1321 tcgcaaaggc tggacgagtg ggagagtgcc ttcgcggaat ggcagagcat gtgtggtctt
     1381 atgcaacggg agaacgcgat gctgaacgag cgcgtgtcgg acttgagcac gcaggtgctg
     1441 agtttaagcg agcagctgcg ccgcttgtcc gggagctgaa tgccatcgag ctccggcaca
     1501 gggagcatga gtatcagaaa acactggagc tggagcacca gccgcagaaa acctaccgtg
     1561 gcccaacgct gggctgacgg cccgtgacaa accccaagaa gaagggctca taaatttccc
     1621 gctaaatacg gtcgtgatgg aaacatcaca gcgtatacaa cggagggatt atgagactgt
     1681 ttaacgtgat tgagacggtg ctgaactgcc tgtacatggc gctggagatc tgggactggc
     1741 tggagaagca ctttcctgtt tttaaggcgt tcgcgctgat tttagtgtga ctttacgcgg
     1801 catgcatctg gctgcatgac tatgcatcag tgtaatatcc agcggctgcg tttctggtgt
     1861 tttttcgtta tgtctgtcgc cactttctcg cgtctggcac cggaaacgca gccaaccccg
     1921 tttcagcggt gcgggcaacg gatgttatgg taaatgtagg gggagaagtg actataagca
     1981 ggggcttcgg tgatggccta aagggatatg gattattgat attactagtt ttctagtatt
     2041 tactagtatg gatgcatcgt aaaaatcaag gaacgcacaa tggcaaaacc tccatcagct
     2101 acaccaccgg atacaccatc ttcatgtgat gttagtgctc tcccaggcat tatccaaaat
     2161 tttggcttaa ttaactttaa cgtatcctgc aataacggtg agctaacggt aacgggtacg
     2221 gatcaaaatg ggaaacacat aagagttaac agctaccaag ggaacggctt taccgaaggt
     2281 acaatgtcaa cgtttcagcc cggaggtaaa gaggccagaa gacttgaggc tgttcgttta
     2341 agagaaagtg ggcttacgca gcaagaaata gcaaaccggt taggcgtttc tcaaaaaact
     2401 ataagtaatg atttaaaatg agtcacttgc tttaagcccg tcttaacggg cttttcatcg
     2461 ctactgaaat gccatgataa atccggcgta gttgctcgtc ccgcagtggt gaaacaccta
     2521 aagatacatt tcagatgttt attttaagcc atggtgcaga tccccatata tgtcgaacat
     2581 caataatgat cttcttgaga tcctttccat tgcatcgtaa tctcttgctc tgaaaacgaa
     2641 aaaaccgcct ggggaggcgg tttttcgaag gttaagtcag ttggggaact gcttaacctg
     2701 gtaactggct tcagcagagc gcagatacca aatactgtcc ttccagtgta gccgtagtta
     2761 ggccaccact tcaagaactc tgtagcaccg cctacatatc tcgctctgct aatcctgtta
     2821 ccagtggctg ctgccagtgg cgttttttcg tgtctttccg ggttggactc aagacgatag
     2881 ttaccggata aggcgcagca gtcggactga acggggggtt cgtgcataca gtccagcttg
     2941 gagcgaactg cctacccgga accgagtgtc aggcgtggaa tgagataaac gcggccataa
     3001 cagcggagtg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccatc
     3061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg
     3121 tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct tcgccgcggc
     3181 cttctcgctt ctctgctaag tatcttcctg gcatcctcca ggaaatttcc gccccgtccg
     3241 taagccattc ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3301 gcggaatata ttctgtatca catattccac ttacgctccg ctgctgcatt tttctcctgc
     3361 cacatgaagc acttcactga tatccgcatc cgtgccaaca tagtaagcca ctatacactc
     3421 cgctagcgct acgtgactgg ttcagggctg cgccccgaaa cccgccaaaa gcctgacgcg
     3481 cctgcggctt gtccaacccc gcgccgaccg ggaaaggctt tcccgcccgt cccggcgtta
     3541 tcaggaggcc ggtttgtcag agaaacggag caagatgctc accatgtggg tgacggagga
     3601 cgagcaccgg cggctgctcg agcgctgtga cggcaggcag ctggcggcgt ggatgcggca
     3661 gatctgcctg gacgaaaagc cggcacgttc cggaaagctc ccctcactct cgccggcgct
     3721 gctgcgccag cttgccggca tgggcaataa cctgaaccag atagcccgcc gtgttaacgc
     3781 cggcggcggc accggccatg acagggtaca
//
