LOCUS       NZ_OW849159             4783 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Serratia marcescens isolate 0 plasmid P4, complete sequence.
ACCESSION   NZ_OW849159
VERSION     NZ_OW849159.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7962736
            Assembly: GCF_905232415.2
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/02/2023 07:57:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,348
            CDSs (total)                      :: 5,217
            Genes (coding)                    :: 5,135
            CDSs (with protein)               :: 5,135
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 29 of 83
            Pseudo Genes (incomplete)         :: 53 of 83
            Pseudo Genes (internal stop)      :: 14 of 83
            Pseudo Genes (multiple problems)  :: 10 of 83
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW849159.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /isolate="0"
                     /isolation_source="Complejo Asistencial Universitario de
                     Salamanca (Salamanca)"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="P4"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(4662..4783,1..121))
                     /locus_tag="LQ123_RS26660"
     CDS             complement(join(4662..4783,1..121))
                     /locus_tag="LQ123_RS26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            308..625
                     /locus_tag="LQ123_RS26665"
     CDS             308..625
                     /locus_tag="LQ123_RS26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            828..1184
                     /locus_tag="LQ123_RS26670"
                     /old_locus_tag="AI2618V1_5095"
     CDS             828..1184
                     /locus_tag="LQ123_RS26670"
                     /old_locus_tag="AI2618V1_5095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(<1504..1626)
                     /locus_tag="LQ123_RS26675"
                     /pseudo=""
     CDS             complement(<1504..1626)
                     /locus_tag="LQ123_RS26675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2277..2600
                     /locus_tag="LQ123_RS26680"
                     /old_locus_tag="AI2618V1_5096"
     CDS             2277..2600
                     /locus_tag="LQ123_RS26680"
                     /old_locus_tag="AI2618V1_5096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2809..>3243
                     /locus_tag="LQ123_RS26685"
                     /pseudo=""
     CDS             2809..>3243
                     /locus_tag="LQ123_RS26685"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3278..3769
                     /locus_tag="LQ123_RS26690"
                     /old_locus_tag="AI2618V1_5097"
     CDS             3278..3769
                     /locus_tag="LQ123_RS26690"
                     /old_locus_tag="AI2618V1_5097"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3776..3991
                     /locus_tag="LQ123_RS26695"
                     /old_locus_tag="AI2618V1_5098"
     CDS             3776..3991
                     /locus_tag="LQ123_RS26695"
                     /old_locus_tag="AI2618V1_5098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1989..2162
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(1514..2022)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          2278..2594
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
     Typing          2591..4083
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atttataaat cccttcatat tcacatgttt ctcccggctt atattcagtg tcaaaaaccg
       61 agttaaactt cgtatctgct tcaaaaatct tgcttttgtt tcctgagcga acaagagcca
      121 tgcaacctcc aaattatcat ttattttgca cacccgcact gtgcatgagt gcattctaca
      181 gcatagccat tgattgcaca agtgcactgt gctgttgtgc atttgatatg tttgctgtac
      241 tatatcggag gtaagaaggt gatcgaaaaa attgccaagt tgcggcaaaa attgaggtaa
      301 aagaaaaatg cagcaagatc atgaacatga tgattgccgc attcagtttc gagacgattg
      361 gctataccag ttttacaaat atggtaaagc caatcgaaag attccaactg cgattagtgc
      421 ttcattaggt cgcaagctgg atatgctgaa tgctgctgat aaagttggag atttgcggtc
      481 tcccccggga aaccgatttg agaatctcaa ccctccattg acagggtatt catccatacg
      541 ggtgaatgag aagtaccgct tgatatttaa gtggggtgaa aacggagtcg aggatttgta
      601 tctcgatcca catgattacc gatagataac aggtcgtggc tgttatctta taaaacaagt
      661 aggtgaggcg ttccctcacc tacattaaaa tgtcattaca aataatgatt aataaaaaaa
      721 atgacgtgac tatatttaag ataattactc atcgctacag ctggtaatag acgaaatact
      781 tatcttcctc atctaacaga gagaagtaaa aaatttagga gtaaagcatg tcccaagcaa
      841 ctagaaaacc atcaactgta ggggatatta ttctttatga atatctcgaa ccaagtggtt
      901 taaaaatcaa tgacttggct gaaatgttga atgtgcatag aaactctgcc agtgctttag
      961 ttaacaataa tagaaagctg tctaccgata tggcttttag gttggctaaa gccttcgata
     1021 ccactgcaaa gttctggcta aatatacagc agtctgttga tttatgggag gttactagta
     1081 gccctcgatt gcaagaagaa atagacagag tgattcccgc tcatgaaatc atagaaagga
     1141 aacacattgt tgatagttct gagaagaaac atgctattgc atgatttttt ttaaaaaaat
     1201 ataagttatc cctcaaaaag aaggcttcta taactagaag ccttcttttt gagggataaa
     1261 aacgctcatg ttaactttct tcttacgatc ttcttgagat ccgtgctctg tacgcgtaat
     1321 ctcttgctct gtaaacgaaa aaaccgcctg gggaggcggt ttttcgaagg ttaagtcagt
     1381 tggggaactg cttaacctgg taactggctt cagcagagcg cagataccaa atactgtcct
     1441 tccagtgtag ccgtagtcag gccaccactt caagaactct gtagcaccgc ctacatacct
     1501 cgctctgcta atcctgttac cagtggctgc tgccagtggc gttttgtcgt gtctttccgg
     1561 gttggactca agtcgatagt taccggaaca ggcgcagcgg tcggactgaa cggggggttc
     1621 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ccgagtgtca ggcgtggaat
     1681 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
     1741 agcgcacgag ggagccatca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1801 gccaccactg atttgagcgt cagattctgt gatgctcgtc aggggggcgg agcctatgga
     1861 aaaccggctc tgccgtggcc ttatcgcttc cctgttaagt ttcttcctgg catcttccag
     1921 gaaatttccg ccccgttcgt aagcccgcac cgctcgccgc agccgaacga ccgagcgcag
     1981 cgagtcagtg agcgaggaag cggaatatat cctgtatcgc atattctgct gacgcgccgt
     2041 tgccgccttt tttctcctgc cacatgaagc acttcactga tttccgtatc cgtgccaaca
     2101 tattaagcca gtatacactt cgctagcgct acgtgactgg ttcagggctg cgccccgaaa
     2161 cccgccaaaa gccactgacg cgcctgcggc ttgtccaacc ccgcgccgac cgggaaaggc
     2221 tttcccgccc gtcccggcgt tatcaggagg ccggtttgtc agagaaacgg aacaagatgc
     2281 tcaccatgtg ggtgacggag ggcgagcacc ggcggctgct tgagcgctgc gacggcaggc
     2341 cgctggcggc gtggatgcgg cagacctgcc tggacgaaaa gcctgcgcgc accggaaagc
     2401 tcccctcact ctcgccggcg ctgctgcgcc agcttgccgg tatgggcaat aacctgaacc
     2461 agatagcccg ccgtgttaac gccggcggcg gcaccggcca tgacagggta cagattgtgg
     2521 cggcgctgat ggccatcgat gccgggcttg agcggctgcg gcacgccgtt cttgagaaag
     2581 gaacggacga tgatcgttaa atttcatcca cgcggtcgcg gcggtggcgc cgggccggtg
     2641 gactatctgc tgggtaaaga ccggcagcgt gaaggcgcca gcgtcctgca ggggaagccg
     2701 gaagaagtcc gggagctgat tgacgcgtcg ccttacgtga agaaatacac ctccggcgta
     2761 ctgtcgtttg cggaggccga tctgcctccc ggccagcggg aaaaactgat ggccagcttc
     2821 gagcgggttc tgatgcccgg actcgataaa gatcagtaca gcattctgtg ggtggagcac
     2881 gcggacaagg ggcgtctgga gctgaacttt ctgatcccca ataccgaact gctgaccggc
     2941 aggcggctcc agccgtacta cgaccgcgcc gaccggccgc gcatcgatgc ctggcagacc
     3001 atcgtgaacg gcagactggg gctgcatgac ccgaacgcgc cggaaaaccg ccgggcgctg
     3061 gtcacgccgt ccggactgcc gaaaacgaag caggaggcca ctgaagccat cacgcgcgga
     3121 ctgctgaccc ttgcttcgtc cggggagctg aaaaaccgtc aggacgtcac tgaggcgctg
     3181 gaacggtcag gttttgaggt ggtgcgcacc acgaaaagca gtatcagcat tgccgacccg
     3241 gacggggggc gaaacatccg actgaaggga gccatctatg agcagtcttt taacgctggc
     3301 gaaggactta gagcagcaat cgaaagcgca gcagcagagt accggcgaga tgctgaaagc
     3361 cgcattcagc gagcacgagc agtctgtcag agcggaactg agcgcaagcg cgaggagaat
     3421 cagcgacgcc atcctcgcac acgagcagag tatgagcgag gcgatggaga aaaaccggcg
     3481 gagcgtactg cggactgccg ggcggacatg gttcaccatc ctgatggtgt cagcgctgct
     3541 gatcggcacg agcgggagca tactgtggtg gcaggggcag cagataaccg acaattacac
     3601 gcatcttcgt cagcaggaag ataccctggc gaaaatgact gcccggacgt ggggagtgcg
     3661 ctatcaggag agcagcgacg gccggaggtt tcttatcctg ccgcccggga tgcaaaccga
     3721 agccatcccc tacgacggga cgacatggat ccgcctgaaa caggagtagc ggaaaatgac
     3781 agagctggaa aagcagttgc tgagcgcatt agagcagcta cagcaggact actcgcaaag
     3841 gctggacgaa tgggagagcg ccttcgcgga atggcggagc atgtgtgggc ttatgcaacg
     3901 ggagaacgcg gtgctgaacg agcgcgtgtc ggacttgagc acgcaggtgc tgagtttaag
     3961 cgagcagctg cgccgcttgt ccgggaactg aatgccatcg agctccggca gagggagaat
     4021 gagtatcaga aaacactgga gctggagcgc cagccgcaga aaacctaccg cgggccgaca
     4081 ctttaaacca tccaggcagc tgacaacccg cacgaagagg ggctcttatt tctcccgcta
     4141 aataggttcg tgatcaaaaa ggtcacgact tattcaggag cgggaaatga agctgttaaa
     4201 tcttattgaa tcattgctta actgcctgta tctggcggcg gagctgtggg actggctgga
     4261 gaagcatttg tcggttttta aggcattggc gcggatttta gtgtgacttt aacatccatg
     4321 catctggctg catgactatg caccagtgta atattcagcg gctgcgtttc tggtgttttt
     4381 tcgttatgtc tgtcgccact ttctcgcgtc tggcaccgga aacgcagcca accccgtttc
     4441 agcggtgcgc ccaacggatg ttatggtaaa tcagtatcag ctgcagatat aaaaaagccg
     4501 ccaggctggc ggcttgctgt tattttcgtg gctttcctgc atctgagcgt tttcctctga
     4561 cccttccgtc attgttacgg tcacgaggtt gagttccgcc gcctggttta ggaacgacaa
     4621 cgtgtgggtt gagtggtcga cgatctgcca ttttctatac ctcactgagc ataaacaaac
     4681 aattgccaac gaatgttccc ttgcgcgggg gtatgttggt ggtggttttg tggtgggatt
     4741 gttttcccgc ctgcgatagt tacttcttta ccgcaaccgg tac
//
