LOCUS       NZ_OW849141             5193 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Serratia marcescens isolate 0 plasmid P2, complete sequence.
ACCESSION   NZ_OW849141
VERSION     NZ_OW849141.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7962811
            Assembly: GCF_905233025.2
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/02/2023 07:57:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,001
            CDSs (total)                      :: 4,881
            Genes (coding)                    :: 4,850
            CDSs (with protein)               :: 4,850
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 14 of 32
            Pseudo Genes (incomplete)         :: 21 of 32
            Pseudo Genes (internal stop)      :: 4 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW849141.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /isolate="0"
                     /isolation_source="Hospital Vall d'Hebron (Barcelona)"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="P2"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(5079..5193,1..218))
                     /locus_tag="LQ109_RS24960"
     CDS             complement(join(5079..5193,1..218))
                     /locus_tag="LQ109_RS24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_077295872.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDDELKELQKALIESPDKGDLIRQ
                     TGGLRKIRMATGTQGKSGSARVIYFLATEEIIYLVMAYPKSTKDSLTDAEKADLKKLTK
                     LLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(366..602)
                     /locus_tag="LQ109_RS24965"
                     /old_locus_tag="AI2795V1_4759"
     CDS             complement(366..602)
                     /locus_tag="LQ109_RS24965"
                     /old_locus_tag="AI2795V1_4759"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_103809261.1"
                     /translation="MTEMEKQLLSALESLQASYEQQHQAWQESYSSLQHMFETTSQALN
                     RSDRACQHLSNQVNSLRGQVESLSDNLSRLMRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(663..1148)
                     /locus_tag="LQ109_RS24970"
                     /old_locus_tag="AI2795V1_4760"
     CDS             complement(663..1148)
                     /locus_tag="LQ109_RS24970"
                     /old_locus_tag="AI2795V1_4760"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_146052617.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFERLETSISEDLKSSS
                     QSISNAIHEQNGRLTGMIRATATTALIWIVVCSALLIGILSGIIWLQGQIISSRLSDID
                     GYSQQLASLKAKSGAGVTIYSDDSQKNGYLVVLPVKAHGVETYTSTAGNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1198..1617)
                     /locus_tag="LQ109_RS24975"
                     /pseudo=""
     CDS             complement(<1198..1617)
                     /locus_tag="LQ109_RS24975"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1546..1818
                     /locus_tag="LQ109_RS24980"
     CDS             1546..1818
                     /locus_tag="LQ109_RS24980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_230118574.1"
                     /translation="MLNPQTGVLVFVETGHQCLFPFVHKTRLFRLSNVGLLKGQHAGCV
                     GAGKVTAINQFACAFSISPQHAGVTALAIFAQQVINGATARTGTT"
                     /name="hypothetical protein"
     gene            complement(1826..2152)
                     /locus_tag="LQ109_RS24985"
                     /old_locus_tag="AI2795V1_4761"
     CDS             complement(1826..2152)
                     /locus_tag="LQ109_RS24985"
                     /old_locus_tag="AI2795V1_4761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_103767548.1"
                     /translation="MEKRTREIKIRLTDSEHQRLIDRCDRTHLAEWLRQLGLGEHTARK
                     RRLPDVAPELLRQVSGIGNNLNQIARRLNQLDGLTPTDRVSLLAVLNSLDRNLSELLEQ
                     HRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2438..3247
                     /locus_tag="LQ109_RS24990"
                     /old_locus_tag="AI2795V1_4762"
     CDS             2438..3247
                     /locus_tag="LQ109_RS24990"
                     /old_locus_tag="AI2795V1_4762"
                     /inference="COORDINATES: protein motif:HMM:NF027299.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_103791768.1"
                     /translation="MKTNYFTHRLFPSDGSVICEPLKTSFDNMIRNNQNTATMSGEYFV
                     IQHVGGDVYTFIKTNSRDVFRKLDLSNNICVDLSQILTANEKIAFASFFIVKNNLIGFS
                     NTLYSPRIGKLAEIYDLVMLARNNNHNINFEPIANQVTEQEVLNYAHVGRITMKLDKSQ
                     NLIGRLGTLFAGNVSYNDVDSFEIKIIPKRTKDIKDTFSGVMSAMPQEVTSVAVSAKEH
                     IGDVATDLNVIMSNTVCDFVNTSSTTDINTQMENNYTNNSTLRNLGY"
                     /name="hypothetical protein"
     gene            3259..3696
                     /locus_tag="LQ109_RS24995"
                     /old_locus_tag="AI2795V1_4763"
     CDS             3259..3696
                     /locus_tag="LQ109_RS24995"
                     /old_locus_tag="AI2795V1_4763"
                     /inference="COORDINATES: protein motif:HMM:NF027298.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_103767545.1"
                     /translation="MKIKDFITGLTVIALCNGVGFGALYLSKKYLFHTVTFKDNLGNLG
                     SSLVTYSTAMMALLIAMVVVIFGLDGPRLDNFKKGGYLHTTYFIYMITFLELGVTMCLS
                     LLCLSNIQSIKVASYSLTFSMITFLLIGFLGIQLLNIKKNN"
                     /name="hypothetical protein"
     gene            complement(3693..4598)
                     /locus_tag="LQ109_RS25000"
                     /old_locus_tag="AI2795V1_4764"
     CDS             complement(3693..4598)
                     /locus_tag="LQ109_RS25000"
                     /old_locus_tag="AI2795V1_4764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_103767546.1"
                     /translation="MTNLALDLFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRIGAAIDWSDRNAPAPTLTITNPENGHAHLLYALKTSIRTAPDGKMK
                     PLKYAAAVENALRKKLDADVGYSGLICKNPNHGYWKIAVWQPELYTLDWLADSLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERASAYNIKFETPLD
                     ENEVNGIAKSIAKWTFNNFSDSDFKSYVDRTHTSEIQSIRGKKSKGGGRPVNQDSERQK
                     EPWKAIGVSRRQYYLLKTKN"
                     /name="replication initiation protein"
     gene            complement(4793..5077)
                     /gene="nadS"
                     /locus_tag="LQ109_RS25005"
                     /old_locus_tag="AI2795V1_4765"
     CDS             complement(4793..5077)
                     /gene="nadS"
                     /locus_tag="LQ109_RS25005"
                     /old_locus_tag="AI2795V1_4765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_004088972.1"
                     /translation="MSFFDELKASLEEAVEIKNGVKAPARVTRYELADVKALRAQLNVT
                     QDEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELASH"
                     /name="nadS"
     Typing          1827..2019
                     /name="Col(Ye4449)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.268"
                     /coverage="100.0"
                     /evalue="8.220000000000004e-72"
     Typing          complement(305..1836)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.869"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtggccagaa agtaaatcac ccgcgcactg ccgcttttcc cctgtgtgcc ggtcgccatt
       61 ctgatttttc gcagcccacc ggtttgcctg atgagatcgc ctttgtccgg tgattcaatc
      121 agcgctttct ggagctcttt gagctcgtcg tccgtggcga tttgctttat ctgacgggta
      181 aacatcggcg tttcgataaa ctctatcgca tggctcactg tgtcattccc tttgcattat
      241 tgatgtacat tgtacaccaa aataatattg cgtctacgtg ggcatcccgt atgagcggtg
      301 ctaaagcgtc gggccgtggt agcgggtacg ctgcttttcc agtttcagct ggtgttcgtg
      361 ctgttttacg agcgcattaa ccggctgaga ttgtcgctca agctctcgac ctgaccgcgc
      421 aagctgttca cctgattgct caagtgctga cacgccctgt cgctgcggtt cagtgcctgt
      481 gaggtagtct cgaacatgtg ctgcaaactg ctgtagcttt cctgccacgc ctgatgctgt
      541 tgctcgtagc tggcctgtaa gctctctaag gcgctcagaa gctgtttttc catttcggtc
      601 atggttttct gtgtcctgtg ggtcaaaatc gttcaggggc gtttctgtgc gttctggcgg
      661 ggttatttcg cggtgtactt caccaccgtg ttgcccgccg tcgaggtgta ggtttccacg
      721 ccgtgcgctt tcaccggcag caccaccaga tagccgttct tctggctgtc gtcgctgtag
      781 atcgtcaccc ccgcgccgct tttcgctttc aggctcgcca actgctggct gtagccatcg
      841 atgtcgctca ggcgtgagct gatgatctgc ccctgaagcc agattatccc actcaggatg
      901 ccaatcagca gcgcggagca gaccactatc cagatcagcg cggtggtcgc cgtggctctg
      961 atcatgcccg tcaacctgcc gttctgttcg tggatagcgt tgctgatgct ctgcgagctt
     1021 gatttcaaat cctcgctgat agacgtttcc agcctctcga actcgcgctt cacgctctgc
     1081 tcggtatcct gcgcttgctg cttcgatttc tcctcgaagc ccttcgccaa atttaaaatc
     1141 tcgctcataa atcattcctt tcagccggat gtttctgccg cctgacgggt cggcaatact
     1201 gatgctgctt ttggtttccc gcgccacggt gagaccaatg ctctcgatct gagtcaccac
     1261 gtcctgccgg ctgcgtattg cgccctgtgc catcaggttc atcaggccgg cggtcagctt
     1321 ttccgctgcc tgctgtttgt ctcgcggcag gtcgctggcc tgcgtcagcg ggcggcgata
     1381 ggcggggtca ttggggtcgc gcagtccgag tcggtcgttg gtcagcgtct gccaggcgtt
     1441 taccctggga cggtcggcgc ggtcaaagta gggttgcagc cgtttcccgc tctgcaattc
     1501 gatgttcggg atcacgaagt tcagctcaag gcggcctttg tcctgatgct caacccacag
     1561 acaggcgtac tggtttttgt cgagaccggt catcagtgtc tgttcccatt cgtccataag
     1621 acgcgccttt tcaggctcag caatgtcggc ctcctgaaag gacagcacgc cggatgtgta
     1681 ggcgcgggca aagtcacagc catcaatcag ttcgcgtgtg cgttcagcat ctccccgcag
     1741 cacgcgggcg tcactgcgct ggcgatcttt gcccagcagg taatcaacgg ggccactgcc
     1801 cgaaccggca ccacgtgagt gaattttaac gatcacggtg ctgctccagc agttcactga
     1861 gattgcggtc gaggctgttt aacaccgcaa gcagggaaac acgatcagtg ggggtaagcc
     1921 cgtcaagctg gttaaggcgt cgggcgatct ggttgaggtt gttgcctatc ccgctgacct
     1981 gtcgcagcag ttcgggcgca acgtcaggaa gacggcgctt tcgggcggtg tgttctccca
     2041 gaccgagctg acgcagccat tcagccagat gggttcggtc acaccggtct atcagacgct
     2101 gatgctctga gtcggtcaga cggattttga tctctctcgt gcgcttttcc attctcactc
     2161 cgctgagaaa tattcgcacc caaagtgcga aagtttcgca gcggatgccc ggggtttcgg
     2221 ggggcggcga gccccctgaa ccagtcacag acggcacctc gtagagggga cgctgtgtgt
     2281 actggcttaa agcatagtgg agtatgtcag attctgtggg cgctgcaaaa aaaaagacct
     2341 cgaaaagagg ctgttatctg atataggttg ttttggtgca aaaaacgaaa tataaataag
     2401 cgaaaatagc gaacaacaat tgacaggcaa gttgaacatg aaaactaatt actttacgca
     2461 tagacttttc ccgtctgatg gttctgttat ttgtgagcca ttgaaaacca gttttgataa
     2521 tatgattaga aataatcaaa acacagccac aatgtctggt gagtattttg taattcagca
     2581 cgttggtggt gacgtatata cgttcataaa gactaacagt agagatgtat ttagaaagct
     2641 ggatcttagt aataatatat gtgtggattt atctcagata ttaaccgcaa atgaaaagat
     2701 agccttcgct tcattcttca ttgttaagaa caatttaatt gggtttagca atactttgta
     2761 ctcaccaaga ataggtaagc tagctgaaat ttatgacctg gtaatgttag caagaaacaa
     2821 taatcacaat ataaattttg aaccgatagc gaatcaggta acagaacaag aagtacttaa
     2881 ctatgctcac gttggtagaa taacaatgaa gttagataag tctcagaatc tgattggtcg
     2941 cctaggcaca ttatttgcgg gtaacgtttc atacaatgat gttgattctt ttgagataaa
     3001 gataatacca aaaagaacaa aagacattaa agacacattc tcaggagtga tgagcgcaat
     3061 gcctcaggaa gtaacatcgg ttgcagtgtc cgctaaggaa catatcgggg atgtggctac
     3121 tgatctgaat gtaatcatgt ccaataccgt ttgtgacttt gtaaatacga gtagcactac
     3181 agacataaac acacagatgg aaaacaacta cactaacaac tcaactctaa gaaacttggg
     3241 ttattaagag gtaggtatat gaaaataaag gattttatta caggcctcac tgtgatcgca
     3301 ctctgcaatg gagtgggatt tggtgcgctt tacctgtcaa aaaaatacct tttccatacg
     3361 gttaccttta aagacaactt aggtaaccta ggttcgtctc tggttaccta ctcaacagca
     3421 atgatggcat tacttatagc tatggttgtt gtgatttttg gattggatgg gccgagattg
     3481 gataacttta aaaaaggtgg ttatctacac acaacttatt ttatttatat gataactttt
     3541 cttgagcttg gggttacaat gtgcctgtcc ctgctttgct tgtctaacat acaaagtatt
     3601 aaggttgcat catactcatt aactttctca atgataactt ttttacttat aggcttcctt
     3661 gggattcaac tcttgaatat aaagaaaaat aattaatttt ttgttttcag aaggtaatac
     3721 tgccttctgc taacaccaat agctttccat ggttcttttt gcctttcact atcctgattt
     3781 acagggcgcc ctccaccttt gctcttttta cctctaattg attgaatttc actagtgtga
     3841 gtcctatcta cataagattt aaaatcagaa tcagaaaagt tattaaaagt ccatttagct
     3901 atacttttag ctatgccatt gacctcattt tcgtccagcg gcgtttcaaa ttttatattg
     3961 taggcgctgg cacgttcata gcaggcttgc agccactggt catactcagg ccagccctgc
     4021 cggatagctc ggtaagccca cttacgagtt ttatcaaaca gggtgcagtt acggcctaag
     4081 ccgtagtcgg caacgatttc tttgtcgttc gctgcgttca ggtcgagcga gtcggccagc
     4141 cagtccagtg tgtagagttc cggctgccag acggcaattt tccagtaccc gtggttcgga
     4201 tttttgcaaa ttaaccccga ataccccacg tcagcgtcca gttttttacg tagcgcgttc
     4261 tcgacagcag cagcgtactt cagcggcttc atttttccgt ctggtgcggt acgtatcgag
     4321 gttttcaacg catagagcag gtgtgcgtgt ccgttttcgg gattggtgat ggtcagcgtg
     4381 ggcgctgggg cattgcgatc gctccagtcg atggcggcac cgatgcggtc aacgtcaaaa
     4441 ccgagccaga acatggcatg aggctggttg aactggatgt atttcgccag aatcgcgcgc
     4501 tcttttcccg cgatgcgtac accaaaatgc agatcgtccg aaaagtacgg tttgtgtggt
     4561 aaacgctcgt tgaacaggtc gagggctagg ttagtcactc acgcctgagc ttatggcggg
     4621 ctgttgtctt gcactgtgca tgtgctaata tccttcttag gttttatgcc tagccctgat
     4681 tgatcatgtc tcttccgcca agttgaaatc atgattatcg gggctttgtt ttttctgccg
     4741 ttaaagttac acgcgaacgg cggcttctgt cgaatccttc cgtcataaag ccttaatgtg
     4801 aggcaagttc ctgaaagaat ttagggttgg cctgtatggt cgccagcacc tttgccgcca
     4861 gtccggtcgg attacgtctg cctgtttccc agcttttgat agtatcgagg ctggtgccga
     4921 gcaccttcgc catttcatcc tgcgtcacgt taagctgggc gcgaagtgcc ttcacgtctg
     4981 ccagttcata gcgtgtgacg cgggctggtg ctttaactcc atttttgatc tcaacggctt
     5041 cttccagaga cgctttcagt tcgtcaaaaa aactcatgct acacctcgtc tttcagtagt
     5101 ttggtcagct ttttcaggtc ggctttttcg gcgtccgtca ggctgtcttt ggtgcttttc
     5161 gggtaggcca tcaccagata gataatttcc tcg
//
