LOCUS       NZ_OW849067             2102 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Escherichia coli isolate 131 plasmid P3, complete sequence.
ACCESSION   NZ_OW849067
VERSION     NZ_OW849067.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7853286
            Assembly: GCF_905219315.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 01:57:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,357
            CDSs (total)                      :: 5,241
            Genes (coding)                    :: 5,005
            CDSs (with protein)               :: 5,005
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 86 of 236
            Pseudo Genes (incomplete)         :: 166 of 236
            Pseudo Genes (internal stop)      :: 45 of 236
            Pseudo Genes (multiple problems)  :: 53 of 236
            Pseudo Genes (short protein)      :: 3 of 236
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW849067.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="131"
                     /isolation_source="Hospital Universitario Gregorio Maranon
                     (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P3"
                     /geo_loc_name="Spain"
                     /collection_date="2016"
     gene            complement(162..428)
                     /locus_tag="LQ135_RS26665"
                     /old_locus_tag="AI2721V1_5093"
     CDS             complement(162..428)
                     /locus_tag="LQ135_RS26665"
                     /old_locus_tag="AI2721V1_5093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(432..1397)
                     /locus_tag="LQ135_RS26670"
                     /old_locus_tag="AI2721V1_5094"
     CDS             complement(432..1397)
                     /locus_tag="LQ135_RS26670"
                     /old_locus_tag="AI2721V1_5094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_230181340.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWFTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(773..1004)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 catttgacat cggattgtct atcctgcatt ggtgtcgagt tggcaaggct gaggataatg
       61 ctgaggataa tgctgaggat aatgcaggat agacagagca ctagagattg catgcaatct
      121 cgtacctatc acctcagcct gccgctggct cttttccaga actaagaatg cgttcttaaa
      181 aatccaaact tgataccgtt taaatctatt ttcacaaaaa gagtttttac cctctcgttt
      241 gttttctgaa tatataaaat taattcatcc tcaaaacctt cgtgcggatg aaaaccaaat
      301 gtgccaaaac ttaatccatc aataagcaaa ataaggtttt taaacctgtc aggaatcacc
      361 gcttcacttc ttaaaaaaaa taaaccaata gtcttatttc cattcaggta ttcaatatta
      421 atattcatac gttaatccga tttatccttc tcaggggcgc gtttgtaacg ccgtttacct
      481 gaatcccaga caaacgccgt ccgcttcccg tcatcagtgc catcccctac atcgtcggca
      541 atgacaagat cctcattggt ttctcgatcc aactggagga cgttttttag cgccccaccg
      601 gtcgagataa aacggcgctt gtgaagctgc cgcgtcagct caagaaacca ctcaggatcg
      661 tttgccatat cttccggttt aacggagtat ttcagcgttt cagcaaccgc gctttgcagt
      721 tgctcggcaa cgttggccac aacctcacct gtcttagttt ttactgcccg aatatcgata
      781 ttcggctcat agttcacccg caagcaatcg cgccagagtt ctacccaacg ttcgtgctta
      841 acgtagttct tccctttaaa ccaagaaggt tgcaccatca gcagacagtg aaaatgcgga
      901 tgtgcgctgc catccttacc tcgcgtcacc tccgtagccc tgatccaccc ctgaacaggt
      961 gatagctcct ttcgcttttc cattcgctta aacgccgcat tcattgctgt aaggactgtt
     1021 ccaagttcac ctatctcgca gttcctgaca gttaacgtca gaaacaacca tcgggaagac
     1081 gggtaatcca caacgatttt cggtagagcc tgataaaaac gggcttgcca catgagggtt
     1141 cttctccact ggcagacagg gcaatgacgg acacggcaaa attgcgcact acgaagccgt
     1201 aggcgcgttt ctccggtttc catgatggta aaccaaccaa aacgtaacaa accgctacag
     1261 gaggccatac ggagtgcata gcgctcgaat tcagcagcag tctggtagat acccccgaca
     1321 gaatcggtaa gcgtgcgctg ggtatcccaa actgcatcac gggggctgta gtccgaaagg
     1381 aatttatctt ctgacattaa gcctgctccg atctgggcag gctgtgattt gatgtggtat
     1441 attcatcacg tacctgtgca ttgcgagcca gtacatgtca ccagagtttg ccgctgaggt
     1501 gacacattcg aaatgttggg gagtgttgac gcactcccca acgccgatta aaaacgcctc
     1561 aattctgctc cattttcttt gacctgtcaa aacccactca aagcctcgtc attactgggc
     1621 taaaccacta tttcatatcc gccatttatc ccttaagtat aaatacaagg atcacgcgcg
     1681 agaattgcgc aaaaaataac cacaatttta acccccccaa aacaagcacc cccgaacggc
     1741 ttaccccaac cttttccaca cacccgatca ccaaaacagg gaataaaaac aaaaccctgt
     1801 cttacaggtg gatggcctca cttcgtgaaa ccatccaccc cgcataagtt ttttaatcgg
     1861 tggtgtcgtc aatcactaaa tcggaacccc aaaggggacc cccacgattt agcatttgac
     1921 gagattaact ctcggagatg gcatcatgct tttaacggac tttaaaaaac ggtgaacgga
     1981 tgcggatttt aatccgtgtc tgtgaggcgt tgagcaacga gcaaagcgag gcggtagaaa
     2041 gagagccagc ggcaggctca ggacgataat ccggggaaaa tgtaccactc gcagagtgtt
     2101 aa
//
