LOCUS       NZ_OW848985             2317 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Escherichia coli isolate 23 plasmid P5, complete sequence.
ACCESSION   NZ_OW848985
VERSION     NZ_OW848985.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7853282
            Assembly: GCF_905219285.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:08:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,105
            CDSs (total)                      :: 4,985
            Genes (coding)                    :: 4,703
            CDSs (with protein)               :: 4,703
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 88 of 282
            Pseudo Genes (incomplete)         :: 196 of 282
            Pseudo Genes (internal stop)      :: 45 of 282
            Pseudo Genes (multiple problems)  :: 47 of 282
            Pseudo Genes (short protein)      :: 4 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW848985.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="23"
                     /isolation_source="Hospital Infanta Leonor (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P5"
                     /geo_loc_name="Spain"
                     /collection_date="2016"
     gene            68..181
                     /locus_tag="LQ157_RS25580"
     CDS             68..181
                     /locus_tag="LQ157_RS25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(562..1098)
                     /locus_tag="LQ157_RS25360"
                     /old_locus_tag="AI2715V1_4875"
     CDS             complement(562..1098)
                     /locus_tag="LQ157_RS25360"
                     /old_locus_tag="AI2715V1_4875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150440.1"
                     /translation="MKKVYTAIILIVLLCGGVLSANYIFLQRHMNEVLKEDPRNDGISV
                     WVYYKWFVNSSEINYDLRSVSAENSSLDVSRVMLQFAEKVKDYDFSKVYLSYRGKDKFY
                     LKGEYFKTLGQEYGIQNPVYTLRTIPENVYMLNGERAYSVWEGGLLGVMGKQMEDLSDF
                     SKAWYLDDFIKSMSD"
                     /name="hypothetical protein"
     gene            complement(1387..1623)
                     /locus_tag="LQ157_RS25365"
                     /old_locus_tag="AI2715V1_4876"
     CDS             complement(1387..1623)
                     /locus_tag="LQ157_RS25365"
                     /old_locus_tag="AI2715V1_4876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(1854..2032)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.066"
                     /coverage="83.0"
                     /evalue="3.53e-41"
     Typing          2..383
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.482"
                     /coverage="99.0"
                     /evalue="1.88e-87"
     Typing          2142..2317
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.413"
                     /coverage="87.0"
                     /evalue="2.45e-70"
ORIGIN
        1 ccgcgtttat ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
       61 ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gccttatccg gtaactatcg
      121 tcttgagtcc aacccggtaa gacatgcaaa agcgccactg gcagcagcca ctggtaactg
      181 atttagagga gatagtcttg aagtcatgcg ccagttaagg ctaaactaaa cgaacagttt
      241 tggtgactgc gctcctctaa ctagccagtt acctcggttc aaagagttgc tgctctgaga
      301 accttcgaga aaaccgcctt gctgggcggt tttttcgttt acagagcaag agattacgac
      361 gcaacagaaa ggatctcaag aagttccttg ctgtattgct actatcctag agatctgacc
      421 cattaggggc attatcagat gacggccttg taatccacgg ggagcaggtc acacatctaa
      481 agcatctacg agtaaaaaca ccccagtgac caatgagcga tttgcgtgag ctaatcgtca
      541 atgtatactg atcgcttcaa attaatcgct catgctttta atgaaatcat ctaagtacca
      601 agccttagag aaatcactaa gatcttccat ctgttttccc attacaccca gaagcccacc
      661 ctcccaaaca ctataagctc tctctccatt taacatatac acgttttccg gaattgttct
      721 taatgtatat acggggtttt gaataccata ttcctgccca agagttttaa aatactctcc
      781 ttttaagtaa aacttatctt tccctctgta actaagataa accttactaa aatcataatc
      841 tttcactttt tcagcaaatt gcagcatcac cctactaaca tccagtgaac tattttctgc
      901 agaaacagaa cgaaggtcat aattaatctc ggaagagttt acaaaccact tataataaac
      961 ccaaacgcta ataccatcat tccttggatc ttctttcaga acctcattca tgtgcctttg
     1021 aagaaaaatg tagttggcag ataaaacacc accacataag agtactatta atatgattgc
     1081 tgtatatact tttttcattt ctataatcca tctcaactgc tctaaagttt accctgagat
     1141 ttttaagcta aggtgccata agttagcaag gaattaagca tcgatttacc ataacgtctt
     1201 cttacgcgca ccgcgaaaac ggcctcaggg cgtttctgcg ccttcagcca caaaagagca
     1261 cgtcttaccc ccagaaaacc gcccggaatc gctctggtga gttttacggt ggcgaatgac
     1321 tatgcaccgg agtgaatacc acgctgtttc gccctgaact ccccacaaaa ccgacgaaaa
     1381 tggcaatcag cgacgcggcg cccgcggacg cgaccaggta aattccgcct gctgccgctc
     1441 ctcctcacgc gcagatcgct gccgttctgc ctggcgctgc gattcgatct gtgcacgagc
     1501 cctgttaagg acaatgtgcc tgttgggctg attcagcttc ttgcctgacg ctctctcagc
     1561 ggcggcaaca gcatcgtcga gaaatctttt ctcggcaggc gtcaggttta tcagtcgttt
     1621 catggcgttc tccggtcaga ataactctgc acagtctgcc agacacggcg aacctcatga
     1681 agaacctgca gccgggaacg gccctgagaa acggtgaacg ggaacggatt ttaatccggt
     1741 gccgtgaagc gttggcgggg tatcggggcg cagccctgag atatcgtttt ggtgccggca
     1801 tcatgatggc ggtactccaa aaacacatct tgtcccgctt tcagtttctg aatttgcggg
     1861 ttccgggcgc agcgccggac cagtcacgta gcgctagcgg agtgtatact ggcttaatat
     1921 gttagttggg cgtggtggct cagggaagtg cttcatgtgg caggggaaaa atgcggctgc
     1981 ggtgcgtaag tgcaaaatgc tgatgatggt gaaatgacgc ctcctcgctc actcggtcgc
     2041 tacgctcctg ccgtgagact gcggcgggcg ttacctgctc acgaatgacg aggtaacgaa
     2101 atgaagctga ggtcaagaag ggcccgaaaa aggatgcggc gctgccgttt ttccataggc
     2161 tccgcccccc tgacaagcat cacaaaatct gacgctcaaa tcagtggcgg cgaaacccga
     2221 caggacttaa agatcccagg cgtttccccc tggtggctcc ctcgtgcgct ctcctgttcc
     2281 tgcctttcgg tttaccggtg tcattccgct gttatgg
//
