LOCUS       NZ_OW848984             5252 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Escherichia coli isolate 23 plasmid P4, complete sequence.
ACCESSION   NZ_OW848984
VERSION     NZ_OW848984.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7853282
            Assembly: GCF_905219285.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:08:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,105
            CDSs (total)                      :: 4,985
            Genes (coding)                    :: 4,703
            CDSs (with protein)               :: 4,703
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 88 of 282
            Pseudo Genes (incomplete)         :: 196 of 282
            Pseudo Genes (internal stop)      :: 45 of 282
            Pseudo Genes (multiple problems)  :: 47 of 282
            Pseudo Genes (short protein)      :: 4 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW848984.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="23"
                     /isolation_source="Hospital Infanta Leonor (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P4"
                     /geo_loc_name="Spain"
                     /collection_date="2016"
     gene            218..331
                     /locus_tag="LQ157_RS25325"
     CDS             218..331
                     /locus_tag="LQ157_RS25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045907702.1"
                     /translation="MHEPPVQSDCCALSGNYQLESNPERHDKAPLAAATGN"
                     /name="hypothetical protein"
     gene            772..1446
                     /locus_tag="LQ157_RS25330"
                     /old_locus_tag="AI2715V1_4869"
     CDS             772..1446
                     /locus_tag="LQ157_RS25330"
                     /old_locus_tag="AI2715V1_4869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_230132639.1"
                     /translation="MKMQHNSSDIDLIELFLFLKTKIASIILFVIFSLILSFTFLFITK
                     DKVIIKYQLNMINNSPSMIINCGDDFYCKANAIQSIINNKSKSITSSIDEKAQKINLSW
                     SGDVRDKPVLIAEVNTIHRAIDDWYIQDYQNYKSIVNNQSNNYINGSEIYAKIALLTNS
                     NTAGENKFISISNETVSKSYKPALFMTLSLILSVLFSSAYHITKRSVLEYKNKFNRSFD
                     NL"
                     /name="hypothetical protein"
     gene            2148..2579
                     /locus_tag="LQ157_RS25335"
                     /old_locus_tag="AI2715V1_4871"
     CDS             2148..2579
                     /locus_tag="LQ157_RS25335"
                     /old_locus_tag="AI2715V1_4871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_032623639.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKNGRLSSSDDFPVSRSA
                     DNHELTHHIELMKKELKENQEYLDESFKRLSAELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(3108..3323)
                     /locus_tag="LQ157_RS25340"
     CDS             complement(3108..3323)
                     /locus_tag="LQ157_RS25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3330..3821)
                     /locus_tag="LQ157_RS25345"
     CDS             complement(3330..3821)
                     /locus_tag="LQ157_RS25345"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032072598.1"
                     /translation="MSNLFTASRDFEEKLKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAISAHEQNMSEAMEKNRRSVLLTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3850..4560)
                     /locus_tag="LQ157_RS25575"
                     /pseudo=""
     CDS             complement(<3850..4560)
                     /locus_tag="LQ157_RS25575"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4499..4822)
                     /locus_tag="LQ157_RS25355"
                     /old_locus_tag="AI2715V1_4874"
     CDS             complement(4499..4822)
                     /locus_tag="LQ157_RS25355"
                     /old_locus_tag="AI2715V1_4874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4936..5109)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          38..538
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.2"
                     /coverage="100.0"
                     /evalue="4.76e-153"
     Typing          5076..5252
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.258"
                     /coverage="96.0"
                     /evalue="1.93e-67"
     Typing          complement(3017..4509)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.6"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4506..4822)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.48e-61"
ORIGIN
        1 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
       61 taaagatacc aggcgtttcc ccctggtggc tccctcgtgc gctctcctgt tcctgccttt
      121 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttat ctcattccac gcctgacact
      181 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
      241 cgactgctgc gccttatccg gtaactatca acttgagtcc aacccggaaa gacatgacaa
      301 agcaccactg gcagcagcca ctggtaactg atttagagga gttagtcttg aagttatgcg
      361 ccggttaagg ctaaactaaa cgaacagttt tggtgactgt gctcctccaa gccagttacc
      421 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa accaccctgg caggtggttt
      481 tttcgttttc agagcaagag attacggctg taacgcaagg atctcaagaa gatcttaaaa
      541 tatgtggaaa ggatgttggc tgacaacaat gccctccaca ggcacaaccg aacaaaaaaa
      601 cgacaaaact tgcattttag gacttttcct acaaacacat aataggataa aatatttttt
      661 tatcttattg gcctttcaaa tgaaatacac cacatcggca aaaattttca tcactgctct
      721 ttttatttca ttcctttatt taacttatgc tttaataaag actattggtg tatgaaaatg
      781 caacacaatt catcggatat tgatttaata gagctatttc tctttcttaa gacaaaaatt
      841 gcgagcatca ttttatttgt tattttctca ttaattttgt catttacctt cctgtttata
      901 acaaaagaca aagtaatcat aaaataccaa ttaaacatga tcaacaattc acccagcatg
      961 attattaatt gtggggatga tttttattgt aaagcgaacg ccatacaatc aataataaac
     1021 aacaaaagca agagcataac atcaagcata gatgaaaaag cacaaaaaat aaacctttca
     1081 tggtctggcg acgtccgtga caaacctgtt ttaatcgctg aagtaaacac tatccatcga
     1141 gctattgatg attggtacat ccaagattat caaaattata aaagcatagt taacaatcag
     1201 agtaacaact atataaatgg ttctgagata tatgcgaaga ttgcattgtt aacaaacagc
     1261 aacactgctg gagaaaataa attcatttca atcagcaatg aaacggtaag caaaagttac
     1321 aaaccagcct tgtttatgac tctatcttta attctttctg ttcttttttc atccgcatat
     1381 catataacca aaagatctgt gcttgaatat aaaaacaaat tcaaccggtc ttttgataac
     1441 ctataacggt taataaccat attcctcata acatccgata tacgccagat gcagaaaagc
     1501 ctactatcag tgggcttttc gataagatct taaatacctc cgggatttca gcgctaagcg
     1561 tttccccttc cgcagcctga cgaagctccc gtagtgctag gccagttacc accattgtta
     1621 aacccgcgaa gcgtccggct taactcctgt cggtttcctt tgttaataag cacacataac
     1681 gtcatagccc tgtctatggc agactcatgt agtagctcac ctttagcgtg gagttcaagt
     1741 acggttgaga tataagccag catttcagcc tcatctgtac tggttaagtt gaggctatat
     1801 ccggtttccg cttccttcag tgcttttgct agatccattt ttaatcctct gggtcgttca
     1861 atactgcaac caaataacat atccaaacga gtcacaatgc accgaaattt accataacat
     1921 ccgtattgcg ccctctggat ggagcaccat ttagagcttt atccacactc ttcaacgccc
     1981 gctattagcc gtatatacca gttaaccatc tcagactcat ccatcaaaaa aatcaatcaa
     2041 aacaatgaaa taacctagaa ttctcatttt gttacattta gtaaatttac taattagtaa
     2101 aacaatataa tctacaaatc aacaaataaa tgagagagac atagagaatg aaaatccccg
     2161 cccacctgaa acataagcca attattcaag tagagaatta tgatcgcatt gatggaccat
     2221 atgccgacga cactgatgca atggggttat ctgttggtat cgcacaatgg aacaccccgg
     2281 gatggacaga gctttcggca aaggtttggc gaaataccgg cgagaagtgg tcccgtcagt
     2341 ctgaggagct tccacttcac cgagtaatag accttgccac tctgatctgc atagcgatgg
     2401 attacagcaa aaatgggcgg ctatcatcca gtgacgactt cccggtcagt cgttctgcag
     2461 acaaccatga actcactcat cacatcgaac taatgaaaaa agagttaaaa gaaaatcagg
     2521 aatatctcga cgagagcttt aaacgccttt cggctgaatt gaaacgtctt ggctactaaa
     2581 aaaagccgcc cagatgggcg gcttttttgt gtctgcagca ccgccgtatt taccataaca
     2641 tctgttgccc gcaccactga aacggggttg gctgcgtttc cggtgccaga cgcgagaaag
     2701 tggcgacaga cataacgaaa aaacaccaga aacgcagccg ctgaatatta cactggtgca
     2761 tagtcatgca gccagatgca tggatgttaa agtcacacta aaatccgcgc caatgcctta
     2821 aaaaccgaca aatgcttctc cagccagtcc cacagctccg ccgccaggta caggcagtta
     2881 agcaatgatt caataagatt taacagcttc atttcccgct cctgaataag tcgtgacctt
     2941 tttgatcacg aacctattta gcgggagaaa taagagcccc tcttcgtgcg ggttgtcagc
     3001 tgcctggatg gtttaaagtg tcggcccgcg gtaggttttc tgcggctggc gctccagctc
     3061 cagtgttttc tgatactcat tctccctctg ccggagctcg atcgcattca gttcccggac
     3121 aagcggcgca gctgctcgct taaactcagc acctgcgtgc tcaagtccga cacgcgctcg
     3181 ttcagcatcg cgttctcccg ttgcataaga ccacacatgc tccgccattc cgcgaaggcg
     3241 ctctcccatt cgtccagcct ttgcgagtag tcctgctgta gctgctctaa tgcgctcagc
     3301 aactgctttt ccagctctgt cattttccgc tactcctgtt tcaggcggat ccatgtcgtc
     3361 ccgtcgtaag ggatggcttc ggtttgcatc cccggcggca ggataaggaa tctccggccg
     3421 tcgctgctct cctgatagcg cactccccac gtccgggcgg tcattttcgc cagggtatct
     3481 tcctgctgac gaagatgcgt gtaattgtcg gttatctgct gcccctgcca ccacagaatg
     3541 ctcccgctcg tgccgatcag cagcgctgac accatcagga tggtgagcca tgtcctcccg
     3601 gccgtcagca gcacgctccg ccggtttttc tccatcgcct cgctcatatt ctgctcgtgg
     3661 gcgctgatgg cgtcgctgat tctcctcgcg cttgcgctca gttccgctct gacagactgc
     3721 tcgtgctcgc tgaatgcggc tttcagcatc tcgccggtac tctgctgctg cgctttcaat
     3781 ttctcctcga agtccctcga ggccgtaaaa agattgctca tagatagctc ccttcagtcg
     3841 gatgtttcgc cccccgtccg ggtcggcaat gctgatactg cttttcgtgg tgcgcaccac
     3901 ctcaaaacct gcaccttcaa gagtgtcagt gacatcctga cggcttttca gctccccgga
     3961 cgaggctaag ctcagtagtc cccgggtaat ggcttctgcg gcctcctgct tcgttttcgg
     4021 cagcccggac ggcgtgacca gcgcccggcg gttttccggc gcgttcgggt cgtgcagccc
     4081 cagtctgccg ttcacgatag tctgccaggc atcgatgcgc ggccggtcgg cgcggtcgta
     4141 gtaaggctgg agccgcctgc cggtcagcag ctcggtattg gggatcagaa agttcagctc
     4201 cagacgcccc ttgtccgcgt gctccaccca cagaatgctg tactgatctt tatcgagtcc
     4261 gggcatcaga actcgctcga agctggccat cagtttttcc cgctggccgg gagggagatc
     4321 ggcctccgca aacgacagga cgccggaggt gtatttctta acgtaaggcg acgcgtcaat
     4381 cagctcccgg acttcttccg gcttcccctg caggacggtg gcgccttcac gctgccggtc
     4441 tttacccagc agatagtcca ccggcccggc gccaccgccg cgaccgcgtg gatgaaattt
     4501 aacgatcatc gtccgttcct ttctcaagaa cggcgtgccg cagccgctca agtccggcat
     4561 cgatggccat cagcgccgca acaatctgca ccctgtcatg gccggtaccc ccgccggcgt
     4621 taacacggcg ggctatctgg ttcaggttat tgcccatgcc ggcaagctgg cgcagcagcg
     4681 ccggcgagat tgagggaagc tttccggaac gtgccggctt ttcgtccagg caggtctgcc
     4741 gcatccacgc tgccagctgc ctgccgtcgc agcgctcaag cagccgccgg tgctcgtcct
     4801 ccgtcaccca catggtgagc atcttgttcc gtttctctga caaaccggcc tcctgataac
     4861 gccgggacgg gcgggaaagc ctttcccggt cggcgcgggg ttggacaagc cgcaggcgcg
     4921 tcaggctttt ggcgggtttc ggggcgcagc tctgaaccag tcacgtagcg ctagcggagt
     4981 gtatactggc ttactatgtt ggcacggatg cggaaatcag tgaagtgctt catgtggaag
     5041 gagaaaaaag gcggcaccgg cgcgtaagca gaatatgcga tacaggatat attccgcttc
     5101 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtac cggcttactg
     5161 gcggggcgga aatttcctag gagatgccaa gaagagactt aacagggaag cgataaggcc
     5221 gcggcggagc cgtttttcta taggctccgc cc
//
