LOCUS       NZ_OW848787             4082 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Escherichia coli isolate 131 plasmid P2, complete sequence.
ACCESSION   NZ_OW848787
VERSION     NZ_OW848787.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7853234
            Assembly: GCF_905218875.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:09:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,078
            CDSs (total)                      :: 4,965
            Genes (coding)                    :: 4,752
            CDSs (with protein)               :: 4,752
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 79 of 213
            Pseudo Genes (incomplete)         :: 147 of 213
            Pseudo Genes (internal stop)      :: 45 of 213
            Pseudo Genes (multiple problems)  :: 50 of 213
            Pseudo Genes (short protein)      :: 3 of 213
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW848787.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="131"
                     /isolation_source="Hospital Universitario Gregorio Maranon
                     (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P2"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(223..2382)
                     /gene="mobQ"
                     /locus_tag="LQ117_RS25305"
                     /old_locus_tag="AI2598V1_4838"
     CDS             complement(223..2382)
                     /gene="mobQ"
                     /locus_tag="LQ117_RS25305"
                     /old_locus_tag="AI2598V1_4838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            2561..2815
                     /gene="mobC"
                     /locus_tag="LQ117_RS25310"
                     /old_locus_tag="AI2598V1_4839"
     CDS             2561..2815
                     /gene="mobC"
                     /locus_tag="LQ117_RS25310"
                     /old_locus_tag="AI2598V1_4839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            3348..4049
                     /locus_tag="LQ117_RS25315"
                     /old_locus_tag="AI2598V1_4840"
     CDS             3348..4049
                     /locus_tag="LQ117_RS25315"
                     /old_locus_tag="AI2598V1_4840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(2025..2383)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2384..2560
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3349..4049
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(227..2469)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcgtttttt ttgtgttagt agtgcgaagg tactattgaa gtgaaaaatt gcgtcaaaag
       61 cgctctaatg cagcgaaaaa agaaccccca ttttttgggg gcggaaaaac aaaactatgc
      121 ttgcattatg tgtgtgcttc ttgcattcat ccctgtcgat gaacatcgaa gaacatcgac
      181 gaacataaca cgatgttaaa caaaacaaaa tattgttcac attcactgtg accaaccgcg
      241 atcatgtctc ctctcggtgg cgtgtgttcg tgcatcttcc tcgcgccgcc attcgtccca
      301 ggtacgatta aaacccagcg agcggaacag atgatccggc tgtttttctg gtgtgttgac
      361 ggcagcaaaa agcggtgcct cacgggtttt cagatgaaac tccataagtt ccggcgcagg
      421 tgatatgtgg ctgtctgcat cgtgatttac ggcaacacca taccctgcat accgtaaccc
      481 cttatcggtc agtgcactga tgaccagctg gctgttatcg gctgagggaa cggcaaacgc
      541 gggatacagc gtaccattaa gccggacaac accgcccaga gagtcatcca cgcccatgac
      601 cagcaaaagc tgaagctggc gggtttcccg gtcgacagaa ctcattaccg gcatcagcgt
      661 cggtagcaga cgtccctgcg taaaatccac cttccttgct gccagtgatg gcatggctgg
      721 ctggcggtct tctccaagac agaaagactc cggccatgtc ttcggctggc ggagattgcg
      781 ggtatattgc ggccatgtct ggcaaacctg cgtcagaagt tgctcaaagg tcgcagagga
      841 ataaacacgt tgcagtggtg gatgttgacc gatttcgctt cggtcacaga tataaccact
      901 cctgtcacgc attaaatcaa cacgctcaaa gattctggtg cggtatttct ccagctcgtt
      961 cccccagagc gtgtattctc cgttccggtt gtgaagcaga aggtaacagg tcatttcccc
     1021 gttaccaggc atgggggcct cgccataccc cgtaacgact gcgctcagta cccctgaatc
     1081 cataacggca ttgtcatttt tcctgagctg ttccgctcgt cgctccgcgc gactacgttc
     1141 tgtatcctcc tgctccttcc ggcgcaattc ctcttcacgt ttaagcaact ggactctgag
     1201 agctgcaagg ccggatgccc cccactgctg ccactgtttt tcaaatgccg tctgactcgc
     1261 gggaatgtcc tgtttctcca ccttccggta atgctgtcgc aatttttcca tttgctggct
     1321 cagcgactgg atttttccct gttcgctacg gtattccggt gccgcccatt gcatcagggg
     1381 aatacgggaa aaaccggacg aaaacggcga tgattttttc aggctggcag catggctcat
     1441 cgcttccttt cgttcggtgt tcagctcttc ccagcgtctg cgcgtcgcct gcatatctgc
     1501 catcatcgac atggcattac tggtgagttt ccatgccgca ttactgtcct tcggcaacgc
     1561 ttcgatgatg tcacgtagtg tgtacgaagt tgcgttgtca ggaagcacga tattcagaag
     1621 gtcagccaca cggcgggcag gctctgtcag cccgctgttt ctgagttcgg tacgggaaat
     1681 ctgtatttcc aggttaagtc tgttaatcag acgattctgt tcgccgcgtt ctgtttcgat
     1741 accgcgtgtt tccatcgcac aggcagactt tccgaggtga ataccaggcg ttttctcaag
     1801 cccctgtcgc tcgtatgaac gatggtctat ctcttcctga taaccggcgt tcgccagtgc
     1861 cctgttcgca tggtcagccc atgatgcacg ccacgtctcg gcatgtgtcc ggtcgttcca
     1921 gtccctgacc tttccaccga atccagcagg tccgacagct ctcgtggtca gcatgatgtg
     1981 cgcatgggga tttgttttat ccatgtgatg gaacgccaca tcagcaatca tgccctgact
     2041 gacaaagttt tctctgacaa aggcgagaac ggtttcccgt gcggcatcct gcggtagttc
     2101 gcgggggatg gcgagttcaa tttcccgcgc aagttgtgag ttcttccggc gttcggcctt
     2161 ctcaacggca ttccagagtt cggcacggtt cgtacaccag gcaggggctg tcgcgggagt
     2221 cagaatcaca gcttcagcca caccgctttt gcgggtgtag tcgtgagtca gtccggtacg
     2281 ctcatcaagc atcagtgaac cggaacgata ggcagcagaa gcaacggcgc tgtagccgct
     2341 gtttctggag atgattttca tgctgagatg ataaatcgcc atttctgccg taatgccttt
     2401 ttgtggtttt ctttcagccc cgcagggcgc acacaatcga aacggagtgg agattgtata
     2461 agtgcgcact tcgtgatttt ccgtgcatta tgccagctca gtttttaaac taccagtgac
     2521 gcgctaaaat gcgatttttc atcgcgcacg gagttacatc atgagcagaa cactcgaaca
     2581 gaagattgct gatgctgagg ccagattaca gcgcctgaag gcgaagagca ggagtctgga
     2641 cacagcgcag aaggtcattg tgggtgcagc attgctggca aaggtcagaa agccggagga
     2701 ggtgcagttg cgtgcctggt tactccagtt cctgaaggca gaggtgacac gacaggctga
     2761 tgtgacgcgc atactgccgc tgattaacga gctggaagcg ctgccaggac agtgattgta
     2821 tcgtccgctc gccgcagtcc cagcgaccga gcgttagcga gcgagtgggc gaggaagcgg
     2881 aagtgaatcc gtcgcctgat tgagcacata cgcacggttt ttcgggaggt gatatgcgct
     2941 gaacccgcat gaatactgga tttccacagg gttggagggg gtgtattcgg aacttgcaat
     3001 tctgcttttt tgcaacgcat tgtttttatt ggttttttgt tttcagcact ccaagttatc
     3061 cacaggctcg tcgttaagtt tttagatctt gtttttaaga tcttgttttt aatacttgtt
     3121 ttagcgccaa aattccttat agaaaacaaa atgttacaat gctataggta atgtgattta
     3181 aggtaaaggt aatatgattt aaggtgttta ggtgggggaa tttaaggtaa aggcaatatg
     3241 atttaaggta atattcacag aaaaagggat attaaaataa aaaggcattg cctttcgttg
     3301 agggtatgtt tatcttttat tcattacctt ttggggaggg atgctctatg tcggatctaa
     3361 ttgcctataa atccaatgcg ctggttgaag ccagttataa attgacccta caggaacagc
     3421 gatttttgtt actttgcatc ggtcgattga agtctggggc tgatgcagaa tctccaaaat
     3481 tgcaaaagac aatgaccatt acagctgcgg aatatttcga ttcattccct gacatggggc
     3541 ggaaaaatgc cgaggttcag ttacaggaag caattgatcg tttgtgggac aggtcaatca
     3601 ttctcaagga tgacgagaaa cgtgaggagt tccgctggat ccagtatcgg gcacagtacg
     3661 ctaaaggcga ggccagagca caaataacat tttctgacgc agtaatgcct tatttgacac
     3721 aactacaagg gcaatttact agagttgtaa tcaagaatat atctagatta tcaagaagtt
     3781 acagtataag gatatacgaa ctcctccagc agttccgcag tactggcgaa cgtattatcg
     3841 ctctcgatga ttttcgctca atgcttgata tagagcataa atatcaaaca tataaatcac
     3901 ttaaccagca attattaaga ccctgcattg acgaactgaa caaaaaaagc gacctggctg
     3961 taacggttga gacgatcaaa aaggggcgca cggttgtagc cctgcatttt cgcttcaagg
     4021 aagacaaaca aatcaagatg acgatctaaa atcgatttta aggcacctaa aacgctccct
     4081 gc
//
