LOCUS       NZ_OU342909             3173 bp    DNA     circular CON 31-JUL-2023
DEFINITION  Escherichia coli isolate IN03 plasmid 1, complete sequence.
ACCESSION   NZ_OU342909
VERSION     NZ_OU342909.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7025511
            Sequence Read Archive: ERR4300391
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Wan,Y. and Wan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2021) IMPERIAL COLLEGE LONDON, Shiranee Lab,
            Department of Infectious Disease, 7N12 Commonwealth Building,
            Hammersmith Campus, Imperial College London, London W12 0NN England,
            United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/31/2023 02:27:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 5
            CDSs (total)                 :: 5
            Genes (coding)               :: 5
            CDSs (with protein)          :: 5
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU342909.1.
            Escherichia coli strain IN03 plasmids.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="IN03"
                     /isolation_source="Urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="1"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2018-07"
     gene            join(3044..3173,1..194)
                     /gene="mobA"
                     /locus_tag="KW122_RS00005"
     CDS             join(3044..3173,1..194)
                     /gene="mobA"
                     /locus_tag="KW122_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(583..1011)
                     /locus_tag="KW122_RS00010"
                     /old_locus_tag="NITRECIN03_24355"
     CDS             complement(583..1011)
                     /locus_tag="KW122_RS00010"
                     /old_locus_tag="NITRECIN03_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            1303..1731
                     /locus_tag="KW122_RS00015"
                     /old_locus_tag="NITRECIN03_24365"
     CDS             1303..1731
                     /locus_tag="KW122_RS00015"
                     /old_locus_tag="NITRECIN03_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(2566..2760)
                     /locus_tag="KW122_RS00020"
                     /old_locus_tag="NITRECIN03_24370"
     CDS             complement(2566..2760)
                     /locus_tag="KW122_RS00020"
                     /old_locus_tag="NITRECIN03_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041622.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2799..3035
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          complement(2007..2531)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.085"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          4..191
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.413"
                     /coverage="99.0"
                     /evalue="1.86e-31"
     Typing          3045..3172
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.1e-19"
ORIGIN
        1 gggcggcagc tctcggtaag aaagttaact cactgactga tgatcgggta ctgaaagaag
       61 ttatgagact gggggcgtta cagaaaaaac tctttatcga cggcaagcgt gtcggggaca
      121 gggagtatgc ggaggtgctg atcgctatta cggagtatca ccgtgccctg ttatccaggc
      181 ttatggcaga ttagcttccc ggagagaaaa ctgtcgaaaa ctgacggtat gaacaccgta
      241 agctcccaaa gtgatcacca ttcgctttca tgcatagcta tgcagcgagc tgaaaacgat
      301 cctgacgcat ccttcctgtt tttccggggt aaaacatctc tttttgcggt gtctgcgtca
      361 gaatcgcgtt cagcgcgttt cagtggtgcg tacaattaag ggattatggt aaatatatga
      421 gctatgcgat aactttaact gtgaagcgat gaacccatta caggcaaagc caattactcc
      481 tgacagtggt ttagccagaa gcagggctac caagaccaat gcaataagta atatatcgtt
      541 ttgctatcgt gcatcgctcg cgctcagttc cattgtgctt ttttaagctg tcgtttttct
      601 tacggtatat accggttttt tatggcgtgg tttcttaact tgttcagcta ctgatggacc
      661 cattgtatct aggtagtcaa ctagctttgt tagatcataa atattgcgac caccatatcg
      721 gcgatcactc ttcgatgttg gtttcttgtc caagagatta gctttttcaa gatcattgat
      781 agctctctga acagtccgta cagaaacccc catacgtatg gctagacttt ccattgacgg
      841 atgcggccac tcttgcaaac tccaccagtg aacgatcagg ttaagtagtg tgttaaaggc
      901 cactgaagtt agctttttct cgttttgtat aaaaaacaat acggtaggca ctgctgtcca
      961 gccaagagac aaaccgccag ctttccattt attcttaacg gagtaagtca ttgattttcc
     1021 taagccccaa aatatttaaa gtatatatta tatggtatat tcatatgaat agggtgacac
     1081 tggcgccatt attgtgcaac caaaaaagac tactctgaaa acgaggaaaa gattttttcc
     1141 tgcctgaatt agatacggag ttagcgatat gaaaaccgaa caacgtcatg atcttgttaa
     1201 agatattgag gtttttggcg tatccttgtc tctgttgatt tccagagcga atgagaagtc
     1261 tgttacaatg ccatctggtc taagtcggga gcagagaaga gcatgggcag cggagcaggc
     1321 gcgcaaaatc cacaattgaa tattgtctca ttctctgaga ccttcaacct ttattacaca
     1381 tccagatatt ctgcaaaaac actcgataaa atcgatgatt tcattgagca ttttgaaaaa
     1441 tacaatctct ttggcgatcc tttaaaagga tatccagctt ggactggcaa agtatcgcca
     1501 tcgtggaaag tgcctgatca ttacgaaaac aaagaagcta ttgagaagta tgctagagct
     1561 aacaaattat ggcatgctca tttaggcgat ccggttttta aagatacgtt tcatgggaaa
     1621 tacaaggttt ctgactgggt tattcatttc cagcggctga caccgaacca tataaagctt
     1681 ttagagcttg gataccatga cccaatgaag ctacctcaaa actttgaatg atcgagcggc
     1741 aggctaaatg aaatcttgag attcattcag tctcgtcgta atctcactat tgtaaaaacg
     1801 aaaaaaccac cctggcaggt ggtttttcga aggttagtta atcctggcag attctctaac
     1861 cgtggtaaca gtcttgtgcg agacatgtca ccaaatactg tcctttcagt gtagcctcag
     1921 ttaggccgcc acttcaagaa ctctcgttac atctctcgca catcctgctt accagtggcc
     1981 gttgccagtg gcgttaagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2041 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2101 acctacaccg aaccgagata ccaacagcgt gagctatgag aaagcgccac gcttcccgaa
     2161 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2221 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2281 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgcctcc
     2341 cgcgcgccct tcttctgggt ctttgtcttt tgctcacatg ttctttccgg ttttatcccc
     2401 cgattctgtg gataaccgta ttaccgcctt tgagtgagct gacaccgctc gccgcagtcg
     2461 aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa gagcgcctta tgtgacattt
     2521 tctccttacg ctcctctatg ccgttctgca tcctgtccgg atgcgttata tcccggtaag
     2581 attttccgct tcaaagcgtg tctgtatgct gttctggagt tcttctgcga gttcgtgcag
     2641 tttctcacac atggcggcct gttcgtcggc attgagtgcg tccagttttt cgagcagcgt
     2701 caggctctga ctttttatga atcccgccat gttgagtacg gcttgctgct gcttattcat
     2761 cttttcgttt tctccgttct gtctgtcatc tgcgttgtgt gattatatcg cgtaccactt
     2821 ttcgactgtt ttgctgccgc tattctgccg cttggctttt tgacgggcat ttctgtcaga
     2881 caacactgtc actgccaaaa aactgccgtg cctttgtcgg taattcgagc ttgctgacag
     2941 gacaggatgt gcaattgtta taccgcgcat acatgcacgc tattacaatt gccctggtca
     3001 gggctttgcc ccgacaccca tgtcagatac ggagccatgt tttatgacaa aacgaagtgg
     3061 aagtaatacg cgcaggcggg ctatcagtcg ccctgttcgt ctgacggcag aagaagacca
     3121 ggaaatcaga aaaagggctg ctgaatgcgg caagaccgtt tccggttttt tac
//
