LOCUS       NZ_OU342908             4082 bp    DNA     circular CON 31-JUL-2023
DEFINITION  Escherichia coli isolate IN04 plasmid 2, complete sequence.
ACCESSION   NZ_OU342908
VERSION     NZ_OU342908.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7025512
            Sequence Read Archive: ERR4300392
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Wan,Y. and Wan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2021) IMPERIAL COLLEGE LONDON, Shiranee Lab,
            Department of Infectious Disease, 7N12 Commonwealth Building,
            Hammersmith Campus, Imperial College London, London W12 0NN England,
            United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/31/2023 02:32:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10
            CDSs (total)                      :: 10
            Genes (coding)                    :: 9
            CDSs (with protein)               :: 9
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU342908.1.
            Escherichia coli strain IN04 plasmids.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="IN04"
                     /isolation_source="Urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2018-09"
     gene            join(3208..4082,1..1285)
                     /gene="mobQ"
                     /locus_tag="KW120_RS00040"
     CDS             join(3208..4082,1..1285)
                     /gene="mobQ"
                     /locus_tag="KW120_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1541..2242)
                     /locus_tag="KW120_RS00045"
                     /old_locus_tag="NITRECIN04_24900"
     CDS             complement(1541..2242)
                     /locus_tag="KW120_RS00045"
                     /old_locus_tag="NITRECIN04_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2775..3029)
                     /gene="mobC"
                     /locus_tag="KW120_RS00050"
                     /old_locus_tag="NITRECIN04_24905"
     CDS             complement(2775..3029)
                     /gene="mobC"
                     /locus_tag="KW120_RS00050"
                     /old_locus_tag="NITRECIN04_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(3031..3207)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3208..3566
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1542..2242)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1282
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3122..4080
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.688"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gatgatggca gatatgcagg cgacgcgcag acgctgggaa gagctgaaca ccgaacgaaa
       61 ggaagcgatg agccatgctg ccagcctgaa aaaatcatcg ccgttttcgt ccggtttttc
      121 ccgtattccc ctgatgcaat gggcggcacc ggaataccgt agcgaacagg gaaaaatcca
      181 gtcgctgagc cagcaaatgg aaaaattgcg acagcattac cggaaggtgg agaaacagga
      241 cattcccgcg agtcagacgg catttgaaaa acagtggcag cagtgggggg catccggcct
      301 tgcagctctc agagtccagt tgcttaaacg tgaagaggaa ttgcgccgga aggagcagga
      361 ggatacagaa cgtagtcgcg cggagcgacg agcggaacag ctcaggaaaa atgacaatgc
      421 cgttatggat tcaggggtac tgagcgcagt cgttacgggg tatggcgagg cccccatgcc
      481 tggtaacggg gaaatgacct gttaccttct gcttcacaac cggaacggag aatacacgct
      541 ctgggggaac gagctggaga aataccgcac cagaatcttt gagcgtgttg atttaatgcg
      601 tgacaggagt ggttatatct gtgaccgaag cgaaatcggt caacatccac cactgcaacg
      661 tgtttattcc tctgcgacct ttgagcaact tctgacgcag gtttgccaga catggccgca
      721 atatacccgc aatctccgcc agccgaagac atggccggag tctttctgtc ttggagaaga
      781 ccgccagcca gccatgccat cactggcagc aaggaaggtg gattttacgc agggacgtct
      841 gctaccgacg ctgatgccgg taatgagttc tgtcgaccgg gaaacccgcc agcttcagct
      901 tttgctggtc atgggcgtgg atgactctct gggcggtgtt gtccggctta atggtacgct
      961 gtatcccgcg tttgccgttc cctcagccga taacagccag ctggtcatca gtgcactgac
     1021 cgataagggg ttacggtatg cagggtatgg tgttgccgta aatcacgatg cagacagcca
     1081 catatcacct gcgccggaac ttatggagtt tcatctgaaa acccgtgagg caccgctttt
     1141 tgctgccgtc aacacaccag aaaaacagcc ggatcatctg ttccgctcgc tgggttttaa
     1201 tcgtacctgg gacgaatggc ggcgcgagga agatgcacga acacacgcca ccgagaggag
     1261 acatgatcgc ggttggtcac agtgaatgtg aacaatattt tgttttgttt aacatcgtgt
     1321 tatgttcgtc gatgttcttc gatgttcatc gacagggatg aatgcaagaa gcacacacat
     1381 aatgcaagca tagttttgtt tttccgcccc caaaaaatgg gggttctttt ttcgctgcat
     1441 tagagcgctt ttgacgcaat ttttcacttc aatagtacct tcgcactact aacacaaaaa
     1501 aaacgcagca gggagcgttt taggtgcctt aaaatcgatt ttagatcgtc atcttgattt
     1561 gtttgtcttc cttgaagcga aaatgcaggg ctacaaccgt gcgccccttt ttgatcgtct
     1621 caaccgttac agccaggtcg ctttttttgt tcagttcgtc aatgcagggt cttaataatt
     1681 gctggttaag tgatttatat gtttgatatt tatgctctat atcaagcatt gagcgaaaat
     1741 catcgagagc gataatacgt tcgccagtac tgcggaactg ctggaggagt tcgtatatcc
     1801 ttatactgta acttcttgat aatctagata tattcttgat tacaactcta gtaaattgcc
     1861 cttgtagttg tgtcaaataa ggcattactg cgtcagaaaa tgttatttgt gctctggcct
     1921 cgcctttagc gtactgtgcc cgatactgga tccagcggaa ctcctcacgt ttctcgtcat
     1981 ccttgagaat gattgacctg tcccacaaac gatcaattgc ttcctgtaac tgaacctcgg
     2041 catttttccg ccccatgtca gggaatgaat cgaaatattc cgcagctgta atggtcattg
     2101 tcttttgcaa ttttggagat tctgcatcag ccccagactt caatcgaccg atgcaaagta
     2161 acaaaaatcg ctgttcctgt agggtcaatt tataactggc ttcaaccagc gcattggatt
     2221 tataggcaat tagatccgac atagagcatc cctccccaaa aggtaatgaa taaaagataa
     2281 acataccctc aacgaaaggc aatgcctttt tattttaata tccctttttc tgtgaatatt
     2341 accttaaatc atattgcctt taccttaaat tcccccacct aaacacctta aatcatatta
     2401 cctttacctt aaatcacatt acctatagca ttgtaacatt ttgttttcta taaggaattt
     2461 tggcgctaaa acaagtatta aaaacaagat cttaaaaaca agatctaaaa acttaacgac
     2521 gagcctgtgg ataacttgga gtgctgaaaa caaaaaacca ataaaaacaa tgcgttgcaa
     2581 aaaagcagaa ttgcaagttc cgaatacacc ccctccaacc ctgtggaaat ccagtattca
     2641 tgcgggttca gcgcatatca cctcccgaaa aaccgtgcgt atgtgctcaa tcaggcgacg
     2701 gattcacttc cgcttcctcg cccactcgct cgctaacgct cggtcgctgg gactgcggcg
     2761 agcggacgat acaatcactg tcctggcagc gcttccagct cgttaatcag cggcagtatg
     2821 cgcgtcacat cagcctgtcg tgtcacctct gccttcagga actggagtaa ccaggcacgc
     2881 aactgcacct cctccggctt tctgaccttt gccagcaatg ctgcacccac aatgaccttc
     2941 tgcgctgtgt ccagactcct gctcttcgcc ttcaggcgct gtaatctggc ctcagcatca
     3001 gcaatcttct gttcgagtgt tctgctcatg atgtaactcc gtgcgcgatg aaaaatcgca
     3061 ttttagcgcg tcactggtag tttaaaaact gagctggcat aatgcacgga aaatcacgaa
     3121 gtgcgcactt atacaatctc cactccgttt cgattgtgtg cgccctgcgg ggctgaaaga
     3181 aaaccacaaa aaggcattac ggcagaaatg gcgatttatc atctcagcat gaaaatcatc
     3241 tccagaaaca gcggctacag cgccgttgct tctgctgcct atcgttccgg ttcactgatg
     3301 cttgatgagc gtaccggact gactcacgac tacacccgca aaagcggtgt ggctgaagct
     3361 gtgattctga ctcccgcgac agcccctgcc tggtgtacga accgtgccga actctggaat
     3421 gccgttgaga aggccgaacg ccggaagaac tcacaacttg cgcgggaaat tgaactcgcc
     3481 atcccccgcg aactaccgca ggatgccgca cgggaaaccg ttctcgcctt tgtcagagaa
     3541 aactttgtca gtcagggcat gattgctgat gtggcgttcc atcacatgga taaaacaaat
     3601 ccccatgcgc acatcatgct gaccacgaga gctgtcggac ctgctggatt cggtggaaag
     3661 gtcagggact ggaacgaccg gacacatgcc gagacgtggc gtgcatcatg ggctgaccat
     3721 gcgaacaggg cactggcgaa cgccggttat caggaagaga tagaccatcg ttcatacgag
     3781 cgacaggggc ttgagaaaac gcctggtatt cacctcggaa agtctgcctg tgcgatggaa
     3841 acacgcggta tcgaaacaga acgcggcgaa cagaatcgtc tgattaacag acttaacctg
     3901 gaaatacaga tttcccgtac cgaactcaga aacagcgggc tgacagagcc tgcccgccgt
     3961 gtggctgacc ttctgaatat cgtgcttcct gacaacgcaa cttcgtacac actacgtgac
     4021 atcatcgaag cgttgccgaa ggacagtaat gcggcatgga aactcaccag taatgccatg
     4081 tc
//
