LOCUS       NZ_OU342907             5627 bp    DNA     circular CON 31-JUL-2023
DEFINITION  Escherichia coli isolate IN04 plasmid 1, complete sequence.
ACCESSION   NZ_OU342907
VERSION     NZ_OU342907.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7025512
            Sequence Read Archive: ERR4300392
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Wan,Y. and Wan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2021) IMPERIAL COLLEGE LONDON, Shiranee Lab,
            Department of Infectious Disease, 7N12 Commonwealth Building,
            Hammersmith Campus, Imperial College London, London W12 0NN England,
            United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/31/2023 02:32:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10
            CDSs (total)                      :: 10
            Genes (coding)                    :: 9
            CDSs (with protein)               :: 9
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU342907.1.
            Escherichia coli strain IN04 plasmids.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="IN04"
                     /isolation_source="Urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="1"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2018-09"
     gene            complement(<87..446)
                     /locus_tag="KW120_RS00055"
                     /pseudo=""
     CDS             complement(<87..446)
                     /locus_tag="KW120_RS00055"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(655..978)
                     /locus_tag="KW120_RS00010"
                     /old_locus_tag="NITRECIN04_24860"
     CDS             complement(655..978)
                     /locus_tag="KW120_RS00010"
                     /old_locus_tag="NITRECIN04_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1042..1233
                     /locus_tag="KW120_RS00015"
                     /old_locus_tag="NITRECIN04_24865"
     CDS             1042..1233
                     /locus_tag="KW120_RS00015"
                     /old_locus_tag="NITRECIN04_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2433..3308)
                     /locus_tag="KW120_RS00020"
                     /old_locus_tag="NITRECIN04_24870"
     CDS             complement(2433..3308)
                     /locus_tag="KW120_RS00020"
                     /old_locus_tag="NITRECIN04_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_000609294.1"
                     /translation="MINMIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSA
                     VILSWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLES
                     VFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLS
                     AAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGIL
                     QYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSV
                     YFLNRKYSEE"
                     /name="DMT family transporter"
     gene            complement(3301..4353)
                     /locus_tag="KW120_RS00025"
                     /old_locus_tag="NITRECIN04_24875"
     CDS             complement(3301..4353)
                     /locus_tag="KW120_RS00025"
                     /old_locus_tag="NITRECIN04_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(4834..5067)
                     /locus_tag="KW120_RS00030"
                     /old_locus_tag="NITRECIN04_24885"
     CDS             complement(4834..5067)
                     /locus_tag="KW120_RS00030"
                     /old_locus_tag="NITRECIN04_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5074..5604)
                     /locus_tag="KW120_RS00035"
                     /old_locus_tag="NITRECIN04_24890"
     CDS             complement(5074..5604)
                     /locus_tag="KW120_RS00035"
                     /old_locus_tag="NITRECIN04_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1319..1406)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1863..2050)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(4..665)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.548"
                     /coverage="99.0"
                     /evalue="3.21e-143"
     Typing          complement(4731..5626)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="4.15e-174"
ORIGIN
        1 ttcttcccgc cgttcggatc tgcaatgctg atactgcttc gcgtcaccct gaccacttcc
       61 agccccgcct cagtgagcgc ctgaatcaca tcctgacggc cttttatctc tccggcatgg
      121 taaagtgcat ctatacctcg cgtgacgccc tcagcaagcg cctgtttcgt ttcaggcagg
      181 ttatcaggga gtgtcagcgt cctgcggttc tccggggcgt tcgggtcatg cagcccgtaa
      241 tggtgattca ccagcgtctg ccaggcatca attctaggcc tgtctgcgcg gtcgtagtac
      301 ggctggaggc gttttccggt ctgtagctcc atgttcggaa tgacaaaatt cagctcaagc
      361 cgtcccttgt cctggtgctc cacccacagg atgctgtact gatttttttc gagaccgggc
      421 atcagtacac gctcaaagct cgccatcact ttttcacgtc ctcccggcgg cagctccttc
      481 tccgcgaacg acagaacacc ggacgtgtat ttcttcgcaa atggcgtggc atcgatgagt
      541 tcccggactt cttccggatt accctgaagc accgttgcgc cttcgcggtt acgctccctc
      601 cccagcaggt aatcaaccgg accactgcca ccaccttttc ccctggcatg aaatttaact
      661 atcatcccgc gccccctgtt ccctgacagc cagacgcagc cggcgcagct catccccgat
      721 ggccatcagt gcggccacca cctgaacccg gtcaccggaa gaccactgcc cgctgttcac
      781 cttcctcgct gtctggttaa ggttattccc gatggcggcc agctgacgca gtaacggcgg
      841 tgccagtgtc ggcagcttcc cggaacgggc aaccggctca cccaggcaga cccgccgcat
      901 ccataccgcc agttgtttac cctcacagcg ttccagtaac cgggcatgtt catcatcagt
      961 aacccgtatc gtgagcatcc tctcgcgttt catcggtatc attaccccat gaacagaaat
     1021 cccccttaca cggaggcatc agtgactaaa caggaaaaaa ccgcccttaa catggcccgc
     1081 tttatcagaa gccagacatt aacgctgctg gagaagctca acgaactgga cgcagatgaa
     1141 caggccgata tttgtgaatc gcttcacgac cacgccgatg agctttaccg cagctgtctc
     1201 gcacgtttcg gggatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     1261 gcttgtctgt gagcggatgc cgggagctga caagcccgtc agggcgcgtc agcaggtttt
     1321 agcgggtgtc ggggcgcagc cctgacccag tcacgtagcg atagcggagt gtatactggc
     1381 ttaaccatgc ggcatcagtg cggattgtat gaaaagtgcg ccatgtccgg tgtgaaatgc
     1441 cgcacagatg cgtaaggaga aaatgccagt tcaggcgctc ttccgcttcc tcgctcactg
     1501 actcgctacg ctcggtcgtt cggctgcggc gagcggactc tcctttctca aaaacgctaa
     1561 ccctgttatc cacagaaccc ggggataaac caggaaagaa catgtgcgca aaaaacgaaa
     1621 ccccgggaaa ggccgcgccg gaggcgcttt ttccataggc tccgcccccc tgacggactt
     1681 cacaaaacag acactttttc ggtgcatctg tgggagcctt gcggctcaac catgaaatcc
     1741 gccagcggtg gcgaaacccg acgtggctta aagatcatca ccgtttccgg tgaatcgctc
     1801 cctcctgcgc ccccacgttc ccgccatatc gattaccgga taccttttcc gcctttctcc
     1861 cttacgggac acgtggcgct ttctcatagc tcacgtcttc tggtatctcg gggcgactta
     1921 ggcagttcgc tccgggctgg gctgtatgca ggaacccccc gttcagcccg actgctgcgc
     1981 cttctccggt aactgttctc ttgggtccaa cccggaaaag acaccacaaa gcgccactgg
     2041 cagcagccat tggtaaaagt catgtgcgag atatttagag agtacttgaa gtggctgccg
     2101 gagtgcggct acactagaag aacaattttg gtgactcgtc tcgcataaga cagttacctg
     2161 gttaagcagt ctgcaagctg acttaacctt cgatcaaacc gccctgcaag gcggtttttt
     2221 cgtttacagg cagcagatta cgcgcagaaa aaaaggatct caagaggatc ctttgatctt
     2281 tttgttacaa acgtctctat ttgactcccc tgagagcgtt ttagcgcgcc tctgaccccc
     2341 ttggttacat tccgacacca gaaacgctcc cgttttttac gtgcatttgg cacggtatcg
     2401 atggaggccg gggggcaatc ctgaacagaa tgttactcct ctgagtattt tctgttaaga
     2461 aaatatacac ttagcacagt tacaataaat cctaccccct gtaagaatgt gaattgttca
     2521 tccaaaaaca accatgacaa aaataatgca actagcggaa taaagtacaa cattatccct
     2581 gccaaatgaa ctgggagcca acgcaaggag ttcaagtaaa aacaaaaagc taaaaaatat
     2641 tgtaatattc cagatgaaaa aacaataatc acggattcca acggaagata aattgatgat
     2701 ttagatataa aaataaagag cgatgacagt ataagggaga atgtcaactg tccgctcaat
     2761 aatgtgatag gctcaaacga tttaataagg cgagacgata taacaacata aaatgctgcc
     2821 gataacacac cggcaacaac aagacaatat ccaacaatat tattcgatcc attactgatg
     2881 tctggcaagg ctaccaaaat actgccaaca attgcaccta taaggaacaa tataaaatat
     2941 tgcggggaat taattttaac tgagcaaata agaacagaca acaccaatat aaacactgat
     3001 tcaagtgaaa aaataattga tgcgcttcct gctgaaataa aataaagtcc gtagagtgta
     3061 aaagtatacg ctaagaatgg ttcaaaaacg cctgtcgcat aagcaagtat cgcttttgag
     3121 tttaatggga gtttttttct ttttataaaa agaactatcc aagatagtat tactgcacta
     3181 caaatctgcc agaaaaacac ccctccggca tctagttctt caactccttt tttgctcatt
     3241 acgatagctg ccccccatga gaacgctgct aaaaaagcaa aaagcattcc tttaatcata
     3301 ttaatcatcc tctttctcat aaaggggttt gttttcaaat ttccttggtt gaaaggagtt
     3361 aaaaaacata gatgcatcat ctacaggaat accaataaac tctccataat aaggatcatc
     3421 tataggaaac tcttctaatg aaacataaaa caatacattc cagttgaaga tcattggatc
     3481 actattttct atttcatgga gcaatctttc atccttagtt aattctcctg gaattaatac
     3541 atcagtatga taaaaaccgc tgtaataaga actattaccg gtgtgcactg ggcatccaaa
     3601 acgatccaaa ttagattctg aatgccatgt tgtagaaatc ccttccggat gtgaataccc
     3661 acccttacca acagactgaa tagtcatgtt acgaatatcc gctaacgctt gttcttgatc
     3721 tttatagaaa cctttttcta tcgccagcct cataaaatgc cccggggcat ttacagaaat
     3781 aagtattccc cctggtattc ttctatcatg atttcttgtt ctatgagcag aagagtaaaa
     3841 aacattaaca ccagccttat atatttttag cccatcttta tcctgaaatg gtacgctttc
     3901 agtataaata acatcctctt ttattggata gcactctggc atatgcagcg aacaaatata
     3961 tttttgtatg tccactaact cttctgatgg acgagcaaag gcaagttgtg gatgaataaa
     4021 ataaatgaga tgcgcactga cctctcctct tcttgcgcgt tctttaaaac gaactctttc
     4081 attacgcaaa aaatcaaaca aatgaccatc atcattatat agttgggggc tatttacaat
     4141 agataaatgg aaatcaagcc ctttaatttt cttgcttgcc aatttaccaa atacacaggg
     4201 ctgattgcct gacacccatt ttcttagtaa atcttcaatt ttatctttag aagtttcgtt
     4261 aaagataaat gaatgaatat tatcaatatc ttttccaaat gaaaaactgg atgataattg
     4321 tggtagttta tttatacgtt gttgaagatt catgaaagct cccacaagac acctcatata
     4381 tttttttata tactgaaact acattatatt tattaccggc gtaaaacgaa ataatttatt
     4441 atcgttaccg atattttaaa aaaatcatta tttgcacagt gttcgagttg ttagtatcac
     4501 tccttcctga aaatccgctc catttaccat aacgtcgcat tgtacgcacc gccaaaacgc
     4561 gttcggtgcg atcacggcag cagacaggta aaaatggcaa caaaccaccc gaaaaactgc
     4621 cgcgatcgca cctgataaat tttaaccgca tgaataccta tgcaaccaga tgctacaggc
     4681 cacattagcc cgccttaatc cactgaacac gtcatttttc atggtttcac catcccagcg
     4741 aagggccatc cagtgtccgc tcctggattt ccggctgacg ctcccgctcc agagataact
     4801 catgttcgcg ttcctgcatc agccgttcat cccttatctc ctgttctcgc tgtataactg
     4861 gctcaagcgt tctgtccgct cgctcaagtg ctgcacctgc tgactcaact gcatgactcg
     4921 cccgttcagc atcgcgttgt cccgttgcgt aagcgaaaac attttctgca attccacgaa
     4981 ggcgttctcc cattcgttca gccgctgcat atagtcctgt tgcagttgct ctaatgcgtt
     5041 cagcaaatgt ctttccagct ccgtcatgct ctgtcactcc tgcaccagtc tcactgcgtt
     5101 cagtttgccg ttgtccttcg tccagttcgt ttcggctttc atccccttcg gcaacaccag
     5161 aaaacgtccg ttgctgtcct cgtgataacg cacgccccat gtcttcgcgt tcagcttctc
     5221 cagactctct ttctgctcgc ggagaatatt catattcgcg tttatcttca tgccctggta
     5281 ccacagcaga ctcccggtcg tggcaaacat cagtactgag acgataatca gccacagcca
     5341 gcgtttcacg ctcattttca gtacggtttt ccagtgcgcg tccatcgtat cgacggtcgt
     5401 tttctgatac ctctccagca gttctttcag ggctgttgta tggtcgttga tagcgttcct
     5461 gattaaagat tcgtttgagg tcagttccct gtttacagat tcttcgagct tcctgaactc
     5521 gtcgttcagc attttctctg tagattcggc tctctctttc agctttttct cgaactccgc
     5581 gcccgtctgt aaaagattgc tcataaaatg ctcctttcag cctgata
//
