LOCUS       NZ_OU016045             1928 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE53_P46 plasmid pl8, complete
            sequence.
ACCESSION   NZ_OU016045
VERSION     NZ_OU016045.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659977
            Assembly: GCF_907177285.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907177285.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 04:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,267
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 38 of 127
            Pseudo Genes (incomplete)         :: 84 of 127
            Pseudo Genes (internal stop)      :: 23 of 127
            Pseudo Genes (multiple problems)  :: 14 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU016045.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE53_P46"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl8"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2018"
     gene            complement(62..1027)
                     /locus_tag="KKQ98_RS16195"
     CDS             complement(62..1027)
                     /locus_tag="KKQ98_RS16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_213313541.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVGK
                     LNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKFHP
                     DKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPV
                     SFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRG
                     KATDWHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLL
                     KEESQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(<1608..1790)
                     /locus_tag="KKQ98_RS16200"
                     /pseudo=""
     CDS             complement(<1608..1790)
                     /locus_tag="KKQ98_RS16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          complement(63..829)
                     /name="rep_cluster_1968"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcacgtcat taaacattga cgtaaattta ctctcactca tctgactttt cttattcggt
       61 atcacttctc tgtcacgtcc aaacccaaaa gccacgtgtc taattctatt ttcaagtcgt
      121 ttgcagattc cttaaacgtc tctcgtaaat gattcgtcaa ctcatcgttt tggctttctt
      181 ctttcaacaa tttgcgcaat ctatatttca aatttctgct aatatacgcc cactctgaat
      241 gatcttgatt cagccctttt atcataattc tatcaacagg ttttacatct ccaggcaagt
      301 aatgaggact tgcaaagcta tctaaacttt cccgaaccat cgcatgccaa tctgttgctt
      361 tgccacgtct tagctgcatt tctatacgcc accatgtttc gatttctttt ggtacaatct
      421 gtttttttcg ttcctgttca agctttttgt tgtacatccg cacttgtcgc tcgctggcac
      481 gactacccca gtacgctgtt tctaatttgc cagaacggcc ataaattggc ttaaaagaca
      541 cagggtcaac gattcgatac tgggtaataa aatcatcagg aacaccgaga atatcgcaag
      601 caatatctgc acgtgagaaa tgcgcatcca gaaacaaatc atgaatgaat tgtttcatcg
      661 acccctttaa aaactcgcca attttatttg gattaaaatc aatacgacct ttgtcagggt
      721 gaaattttaa catttcgata taggcgatat cttctccata ttggtcaaat aattgccaac
      781 catttgtacc aaccgacttc aaataccctt gattttcatt caaacgcatt aattgctcaa
      841 aatcgacaaa cacaactcca tttttcgtcc aataagctat ggtatcattc agttttccga
      901 caattgtaat tcgatcgatt gaccaactta attttagggg gtgtacctta tcgtcaacac
      961 ttaaccgcct gttagaaata cggttaagtt tttcccactc atttacgatt atgccttttt
     1021 tactcaaaaa tagctcctcc tcaacgaaaa aagagattct tggaaagaat ttccagagtt
     1081 cacgtaactc taaaaatcca catccaaaaa tctctagctt gaagaaacct aaaaatattc
     1141 gtataatgaa cttgagttac ggaactaggt gaagtagttt ccgtaaaggt cgagagtgcg
     1201 tcaacactgc tcgactattt ttttactctt ttttctcatt ttctttgatt atagcactcc
     1261 tctagaaaaa ataaaaagac ctcagggaat ttcattcccc gaaccccttg agcctcctcc
     1321 tgccgaaata gaatggcgct gccagcattt atttcgttca gctatcggct acacaatcta
     1381 acacgaccgt tccgctacca gtcgccttac aggctccatt ttcgctcctc taacgtgttt
     1441 caccgattgt gccgataact aaacgaaata aacgctaaaa ccgctcagaa cgcaaaatat
     1501 gccattttag agctattgta gaaaatgtag tttgaaatgc aattttcttc tttcagcttt
     1561 ttatttccca cttgtaaatc tgctaattat tttctaccag acaaactatt gattttttgg
     1621 ctatcagtta actcacgatt attatgagaa atgcttgtat tttttgttcc ctttttgaac
     1681 gaaatactaa accccaagtt aaacccctcc ttcgcgctgt cgctaataaa aagagtgtaa
     1741 acctttctgt tactctttga caaagagtaa cattttccta attgtgacat ggctgtcaca
     1801 attccaaatg ctctccgagg gtattcgctg tcacaagtga caacgaaaaa aggagccgaa
     1861 agcgtgtcac gctctcaact ccttttacgc tttattttgc ttcacgcaaa ataatcgctt
     1921 atcgtaaa
//
