LOCUS       NZ_OU016044             2947 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE53_P46 plasmid pl7, complete
            sequence.
ACCESSION   NZ_OU016044
VERSION     NZ_OU016044.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659977
            Assembly: GCF_907177285.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907177285.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 04:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,267
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 38 of 127
            Pseudo Genes (incomplete)         :: 84 of 127
            Pseudo Genes (internal stop)      :: 23 of 127
            Pseudo Genes (multiple problems)  :: 14 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU016044.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE53_P46"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl7"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2018"
     gene            221..1195
                     /locus_tag="KKQ98_RS16175"
     CDS             221..1195
                     /locus_tag="KKQ98_RS16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002337993.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVGT
                     LRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQE
                     GKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYKYRELL
                     KQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1192..1332
                     /locus_tag="KKQ98_RS16180"
     CDS             1192..1332
                     /locus_tag="KKQ98_RS16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_002322683.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAVV
                     N"
                     /name="DeoR family transcriptional regulator"
     gene            complement(1669..2085)
                     /locus_tag="KKQ98_RS16185"
     CDS             complement(1669..2085)
                     /locus_tag="KKQ98_RS16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322684.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKIEK
                     QLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2087..2368)
                     /locus_tag="KKQ98_RS16190"
     CDS             complement(2087..2368)
                     /locus_tag="KKQ98_RS16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347307.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDF
                     DHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
                     /name="hypothetical protein"
     Typing          246..1192
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.899"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aagaaaacga gaaaaaagag caaaataaaa gccgaacggt gttggaagca cccttcggct
       61 aattacggaa actactcacc tagttccgta actcaaattc attatacgaa tatttttagg
      121 tttcttcaag ctagagattt ttggatatgg atttttagag ttacgtgaac tctggaaatt
      181 ctttccaaaa atctcttttt tctttgagga ggaacaaaat ttgagtaaac gtggcacgat
      241 cataaatgag tgggaaaaac ttaaccgcat atctaacagg cggttaaatg cacaaactat
      301 gtgcaccccc ttaaaaatgc aatggtcaat tgaccgaatt accattgttg gtacattaag
      361 ggaaaatatt tattatcaca ctccccatga tgtgttgatt ttagattttg aacagcttat
      421 gcgtctaaac gaaggtaacg gatatttaaa agcggttgga aataatggct ggcaattact
      481 agaccaacat gaggaaaata tagcttatat tgagattctt aagtttcaag agggaaaagg
      541 gcggattgac tttaacccca ataaaattag ccaatttctg gcgggctcga tgaagaactt
      601 tatccatgat ttatttttag aaccacattt ctcacgtgca gatattgctt gtgacattat
      661 tgacgtgcca gatgatttca ttacccaata tcgggtggtt gatcctgttt ctttcaaacc
      721 aatttacggt cggtctggga aactggaaac agcatattgg ggaagtcgtg ctagtgaacg
      781 acagattcgg atgtataaca aaaaattgga acaagaaaga aaacggaaag ttgttccgaa
      841 agaaatcgat acttggtggc gactagaatt gcaattgcga agggggaaag caactgattg
      901 gcatgcaatg gttcatgaaa gtttagatag ctttgcaagt cctcactatt tgccagaaga
      961 tgtgaaagta actgacagaa tgatgattac tgctcttata acggaacata gttactgggg
     1021 gcaaattcat agaaattcaa aatataaata tcgtgaattg ttaaagcaag aaagtcaaaa
     1081 tgatgagttg acgaatcatt tgcgagaaac attttcggag tcggcagatg atttgaaacg
     1141 agaattagat acatggcttc aaggacttga tgtgacggaa gtaggtgctg aatgagtgat
     1201 tcgatagaaa aaatgaccct aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg
     1261 gtgcgcaatc aactggtcaa ttcgggattt gaccttgctc agtataaaga aaaaaatgca
     1321 gtggtcaatt gaccgaaatt tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg
     1381 ggcgattttc gccccctttg ctctatgtga aaaatttgtt atacttattt tatccttaaa
     1441 acataccaat ctttttctta tttagctgct cgtacgagca gcttttttta cgattatgaa
     1501 ttcaaatcat gtatttataa tttttttgtt tttatctttt tttctgttat ttcttctgaa
     1561 attcgttttt ttcattcaat tggttactat cttattgtaa atgttgctat taagatggtg
     1621 atcctaccca aaaaataaac caggaattta aatctcctgg tttatttttt atctttttag
     1681 aaaatttgtt gggtcgaaat ttttagaagt aaatgtttga ttggtttctt tttgacgatt
     1741 ttttggttga tttcgtttgg agggattaag ttgtttttca attttttctt tcgtttcttg
     1801 atacacttta gaatttgtgc caataagaat acctgtatct tttagatata aatcagcaaa
     1861 ttctgcaatg atttcggaca tgatggaaat ttcttttccc atagcattta gtttggcttg
     1921 gttgctggta gtcgtagtat tggtatcagg ttcataactc atacataaaa aacgaatggc
     1981 ttccgagtaa ttgttgatat ctggatttct ttcgataatg gaatctaaaa cgtccacgtg
     2041 actgggataa agggtaacgt gtttttgaac agaagttttt ctcatgttat cttcctccga
     2101 taagtgtttc aatgagttgg ttgtttgtac gaacatattc attcagcgtt tcagtatttt
     2161 tttcttgaat agcgataaat ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca
     2221 taagtgcgtg gtcaaaatcg ttttcgatta ggacttttaa gtagcgattt cgactccatt
     2281 ttttgccagt tttatttgtg agttcttggc aacgtttgtc tatttttgca ataacctttg
     2341 aatcgatctt ccgaactaaa atgtccatag aaaaactcct ttcgcatttg tgtataattt
     2401 atgtatatca tacgaatata gtatttgtat agctgttttt tgctaaaata ctatacataa
     2461 gctagccacg atggcgaaaa agtgatatca aatttttttc tgagaaacgt gataggcaca
     2521 gcctatctac ccactttttt tagaaagtgg gtacacgttt ccttatgctc tttataaaat
     2581 ggtgatgtgc gacttttttc tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa
     2641 actgaacgat tatgacgggc atgtaatcaa gatgaaatga ttttataagc gcgtattttg
     2701 cgttctaaga cgttttagcg ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt
     2761 aagagaagcg agaatggagc ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg
     2821 caagccgaga actgaatgaa atgaagagcg gaagcgtgaa ataatttcgg caggaggagg
     2881 ctcaaggggt tcggggaatg aaattccctg aggtcttttt atttttttaa gaggagtgct
     2941 ataatca
//
