LOCUS       NZ_OU016041             6501 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE53_P46 plasmid pl4, complete
            sequence.
ACCESSION   NZ_OU016041
VERSION     NZ_OU016041.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659977
            Assembly: GCF_907177285.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907177285.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 04:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,267
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 38 of 127
            Pseudo Genes (incomplete)         :: 84 of 127
            Pseudo Genes (internal stop)      :: 23 of 127
            Pseudo Genes (multiple problems)  :: 14 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU016041.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE53_P46"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl4"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2018"
     gene            join(6144..6501,1..59)
                     /locus_tag="KKQ98_RS16040"
     CDS             join(6144..6501,1..59)
                     /locus_tag="KKQ98_RS16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806723.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDINTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKGTNKTVTSSNFDPTKFLKR"
                     /name="hypothetical protein"
     gene            complement(115..333)
                     /locus_tag="KKQ98_RS16045"
     CDS             complement(115..333)
                     /locus_tag="KKQ98_RS16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002410865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806722.1"
                     /translation="MTKNVNDLFEEYYDNHNLDESRYSHFSKKQLVIEAEYMHDALQNI
                     LKYLDDGGTDLNAIRGEVMDGIYESRI"
                     /name="hypothetical protein"
     gene            complement(336..1076)
                     /locus_tag="KKQ98_RS16050"
     CDS             complement(336..1076)
                     /locus_tag="KKQ98_RS16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_060806721.1"
                     /translation="MTVRITKARHFSFLLYPDSIPNDWKEKLESLGISMAVSPLHDMDE
                     KKDKEKWTIDDVVRNGKHYKKPHYHVIYIARNPVTIESIRKKIKRKLGNNSVAHVEIID
                     SIKGAYEYLTHESKDAKAKNKHIYDKKDILYINDFDIDRYITLDESQKRELRKTLTNII
                     DMYGVVNAKELRAFLNWRGTEFGVTNLGDVEDVLSANGSLFRFYFDGNYQNGFRPQYAK
                     RINSETGEIISGVDELISKTEEGE"
                     /name="replication protein"
     gene            complement(1142..1282)
                     /locus_tag="KKQ98_RS16055"
     CDS             complement(1142..1282)
                     /locus_tag="KKQ98_RS16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016373449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_060806720.1"
                     /translation="MSKKKRLTITLSEQVLEYLDEVAKKKGLSKSALITIALEEYKKGQ
                     K"
                     /name="CopG family transcriptional regulator"
     gene            complement(1768..1947)
                     /locus_tag="KKQ98_RS16060"
     CDS             complement(1768..1947)
                     /locus_tag="KKQ98_RS16060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806718.1"
                     /translation="MEKDIYKQLTQQIQKEKEKGLLDHLDELELKADEINKNRMSAKEK
                     GIKLKDSSLQSDKS"
                     /name="hypothetical protein"
     gene            complement(2070..2294)
                     /locus_tag="KKQ98_RS16065"
     CDS             complement(2070..2294)
                     /locus_tag="KKQ98_RS16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            2659..2943
                     /locus_tag="KKQ98_RS16070"
     CDS             2659..2943
                     /locus_tag="KKQ98_RS16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            2946..3362
                     /locus_tag="KKQ98_RS16075"
     CDS             2946..3362
                     /locus_tag="KKQ98_RS16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060807318.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDINTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(3499..4218)
                     /locus_tag="KKQ98_RS16080"
     CDS             complement(3499..4218)
                     /locus_tag="KKQ98_RS16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            4880..4966
                     /locus_tag="KKQ98_RS16085"
     CDS             4880..4966
                     /locus_tag="KKQ98_RS16085"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_125669474.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5268..5492)
                     /locus_tag="KKQ98_RS16090"
     CDS             complement(5268..5492)
                     /locus_tag="KKQ98_RS16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            5857..6141
                     /locus_tag="KKQ98_RS16095"
     CDS             5857..6141
                     /locus_tag="KKQ98_RS16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     Typing          complement(3500..4218)
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaacaaacaa aacagtcact tctagcaatt tcgacccaac taaatttcta aaacgataaa
       61 aaaacagata ggagttattc ctatctgttt tttatttccc aatattctat tttttcatat
      121 cctactttca tatatgccgt ccatgacttc tccacgaatc gcattcaaat ccgttccacc
      181 gtcgtcgaga tatttcaaaa tattttgcaa agcatcatgc atatactcag cttcaatcac
      241 caattgcttt ttactaaaat gactataccg actttcatct aaattatgat tatcataata
      301 ttcctcaaac aaatcgttta cgttttttgt cattcttatt ctccttcttc agttttactt
      361 attaattcgt caactccact tataatttcc ccagtttcag aatttattct ttttgcatat
      421 tgtggtctaa acccattttg atagttccca tcaaaataaa atctaaacag actaccattt
      481 gcactcaaaa catcttctac atctcctaag ttggttacac caaattctgt acctctccaa
      541 ttaagaaatg ctctaagttc tttcgcatta accacaccgt acatatcaat aatatttgtc
      601 aaagtttttc ttaactctcg tttttggctt tcatcaaggg tgatataacg atcaatatca
      661 aaatcattaa tatacaaaat atctttttta tcgtaaatat gtttgttctt cgctttcgcg
      721 tcttttgact catgagtcaa atactcataa gcacccttaa tggaatcaat tatttcaaca
      781 tgagcaaccg aattatttcc caattttctc ttgatttttt ttcgaatact ttctatcgtt
      841 actggatttc tagcaatata tataacatga taatgcggtt ttttatagtg cttaccattt
      901 ctaacaacat catcaattgt ccacttttct ttatcctttt tttcatccat atcgtgtaaa
      961 ggactaacag ccattgaaat acctaaactc tctaattttt ccttccaatc atttggaatc
     1021 gaatcaggat ataacaaaaa actaaaatgt ctagctttag taattctaac agtcatgata
     1081 taatcacctt atcaattaat gagtgagcgg tcttttagtc gggagcgctc tttttttatg
     1141 ctcatttttg ccctttcttg tattcttcta acgctattgt tattaatgca gatttggata
     1201 aacccttttt tttagctact tcgtctagat attctaaaac ttgttctgac aacgttattg
     1261 tcaaacgttt cttcttactc atgtcattct ccttttcatt atttatcttc ttaaaaagaa
     1321 taccacatat tacgcattaa atgcaactaa aagcgtattt ttttaaaaac ggcggtcgaa
     1381 acggcggtca aatggcggtc gaaaaaaaag gctctatcca ttggtacagt aaggataaag
     1441 cactttttgg cacaaaaatt ggcactcggc acactatggg gggtcgtagt agccccccag
     1501 attttccaaa ttccaaattt tttccaaaaa ttttccagcg ctaccgctcg gcaaatcaag
     1561 accgtacctt tttgaaaaaa ggcaccccaa aaactttcca ctcgccgtta aggcgtcaag
     1621 aacgttccct atggtcacct tcttgtgttt ccagcttttg ggttttgttt atagccgtca
     1681 actccttaaa gcctcaaaga gacgctaaga gggcttatta gcctctctaa gcgtctctaa
     1741 ttcgatttac cgttgaccgc acgttatcta gcttttatcg ctttgcaacg acgaatcttt
     1801 aagttttatg cctttttctt ttgctgacat tctgttttta ttgatttcat cagcttttag
     1861 ttctaattca tctaaatgat ctagcaagcc tttttctttt tccttttgaa tttgttgggt
     1921 caattgttta taaatatctt tttccatccc catcacctcc ttaaaagcta ttattattcg
     1981 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2041 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2101 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     2161 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     2221 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     2281 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     2341 ctttgccgtc tatactctca aaaaaaatgt cgtttgaaaa actaaataaa tttttgatcg
     2401 tgcattttta aatttggtta tcgattacac gtgcagcact ccccccgaat tttaaagagc
     2461 ataaggaaac gtgtacccac tttctaaaaa aagtgggtag ataggctgtg cctatcacgt
     2521 ttctcagaaa aaaatttgat atcacttttt cgccatcgtg ggtagcttat gtatagcatt
     2581 ttggcaaaaa acagctatac aaatatagta tttgtatggt atacataaat tatacataat
     2641 tgcaaaagga gattttttat ggacatttta gttcggaata ttgatccaaa gtttgttgca
     2701 acaatagaca aacgttgcca agaactcacg aataaaacag gagaaaaatg gagtcgaaat
     2761 aaatatctaa aagttttaat cgaaagtgat tttgaccgtc aacttacgac ttacaaaaaa
     2821 gaccaattcg atttattgct cgataaattt attgctatcc aaacagaaaa tactcaaata
     2881 ttaagcgagt atgtctacac aaataatcaa atcattgaaa tgcttatcgg aggaatgata
     2941 taaccatgag aaaaacttct gtccaaaaac atgttacttt gtaccctagt catgttgacg
     3001 ttttagattc catcatcgaa aaaaatccag atatcaataa ctactcggaa gccattcgct
     3061 ttttatgtat gagttatgaa cccgatatca acacgaccac taccagcaat caggcaaaac
     3121 taaatgctat gggaaaagaa atttccatca tgactgaaat catcgcagaa ttcgctgatt
     3181 tatatctaaa agatacaggc gttcttattg gcacaaattc taaagtatat caagaagtga
     3241 aagaaaaaat cgaagctaaa ctaaatccat caaatcgaaa tcgaccaaag aatcatcaaa
     3301 aagaaaccag tcaaacagtc acttctagaa atttcgaccc aacaaatttt ctaaaacgat
     3361 aaaaaacaga taggagttat tcctatctgt tttttattta gaagccccat acaatatgct
     3421 agaatgtact agcaatctta gtcggttgta agggacttgt ctacttgctt aatgtaaggg
     3481 gggcgagttc ttttttattt attcttctaa ccagttacgc agagaaatct ttggcaaatc
     3541 tgacgttagg cttgaaaaag taaattctaa tttggcgatt ttattgccct tttttgcttt
     3601 tatttttgtg actttaagat cattgaagta tttttttagt tctcgtaatg caggatttaa
     3661 tactttttga tcaatgttac ccatttgata gtattctggg atatccatta agctcctaaa
     3721 ctcttcaata cgaactacat aataaccagt agaacggtac tgcataagca gtcggaatag
     3781 agtttttgca tacgtagaac gcaacgacgt gaaagcagat aattcaaatc tactaaattc
     3841 agctgttaaa ccattaataa tatattctag gtccggatta acactgactt caacaaactt
     3901 ttttgtttta ttgatttcaa aaccagtaaa caaaacaaag tagcgaatag aatcctcatc
     3961 ttcagtacgg taagtaagat taagcatatc tttatagagc ttttctaaat caccgacaaa
     4021 ggcttttgta gcagtcatct tataatcact taactctttt aattcctcga aagaaaatct
     4081 aacttttttc aaaccacgat ctttcatacg agcgcataat gcaaaaaata aatccatctg
     4141 cttagcattg aaatttttca gcggtactaa attcaattca ttttgatatt tgactgttaa
     4201 ttccgttttt tcatccattc ctttcttccc cccttacaaa taaataataa cctttaacaa
     4261 ccaactcgta aataggttgg ttgttattat acaaaaggat attagtactc agataaaaag
     4321 ttgttagtac tcagataaaa agttgttagt actcagataa aaagttgtta gtactcagat
     4381 aaaaagttgt tagtaccctt ctgtatccgt tgggagagta aggctcaact gccccctaaa
     4441 aagggttcta aaaaatcttt aaaaaataaa taaaataaac gcgcgcgcgc gagcaaaatt
     4501 tcccgccaaa aaatcaccca ctttttaaaa taaacaggtt cgcgcatgat attaaactgc
     4561 caatctagct ccctatggtc gctgtaagaa ggagtgatcg ctacgctctc acattttact
     4621 tgcagcagtc gcctttaggc tccttcgcca aaaataattc attagggact cctttgtggc
     4681 gtcctgagag ctcgtctaag ccgttctaag acaagattcg ataaaaacta tgtccttcgt
     4741 tctaaagcgc cttacaggcg gtcagggcaa gccctaacaa ccccaaggca ataaaaaact
     4801 taggcttatt tttgcttttt tattgtataa ttgattgtcg cctatccgac agtcttgcga
     4861 ctggaaggag gtgtgtttcg tgttcactaa ctttatttgc tcccttcttg ttgggctttt
     4921 aagcgctttg ttcagttact ggttgaataa ccgaaacaag aagtaaaact taattaaggc
     4981 gactcagccc acaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtgtttc
     5041 gtgttcactt atttgcaatc aaattataac agaaataaat atttatttca aaaatatcct
     5101 acaaaagtct aaaatccgta attatttctt atatgcaaca tttacaaata aagtcgctct
     5161 gagagctcgt atttttcgat ttaagacgtt ttatttagtt tttggtgtat ttcattggtc
     5221 caagctaaaa aactcttaga agcgatttta gagccattta aacccactta attatattct
     5281 ccatgaaatt cgctccatgc gtcaaaagct gcgtctgaaa ttccgccgtc ttcgttcact
     5341 agttgcatta atttttccaa cgatcgtttt ctttcgggtg tcatattttt atattccaac
     5401 aatttttcgt ctgttaaatc gaacttgatt gcttgaattg cttgctctaa tttttctaag
     5461 ttttgaaaat attcgtaagg aaatttttcc attttttctt tctccagtct cacaattttt
     5521 ctagaaataa aatcacttct ttgccgtcta tactctcaaa aaaaatgtcg tttgaaaaac
     5581 taaataaatt tttgatcgtg catttttaaa tttggttatc gattacacgt gcagcactcc
     5641 ccccgaattt taaagagcat aaggaaacgt gtacccactt tctaaaaaaa gtgggtagat
     5701 aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat cactttttcg ccatcgtggg
     5761 tagcttatgt atagcatttt ggcaaaaaac agctatacaa atatagtatt tgtatggtat
     5821 acataaatta tacataattg caaaaggaga ttttttatgg acattttagt tcggaatatt
     5881 gatccaaagt ttgttgcaac aatagacaaa cgttgccaag aactcacgaa taaaacagga
     5941 gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg aaagtgattt tgaccgtcaa
     6001 cttacgactt acaaaaaaga ccaattcgat ttattgctcg ataaatttat tgctatccaa
     6061 acagaaaata ctcaaatatt aagcgagtat gtctacacaa ataatcaaat cattgaaatg
     6121 cttatcggag gaatgatata accatgagaa aaacttctgt ccaaaaacat gttactttgt
     6181 accctagtca tgttgacgtt ttagattcca tcatcgaaaa aaatccagat atcaataact
     6241 actcggaagc cattcgcttt ttatgtatga gttatgaacc cgatatcaac acgaccacta
     6301 ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat ttccatcatg actgaaatca
     6361 tcgcagaatt cgctgattta tatctaaaag atacaggcgt tcttattggc acaaattcta
     6421 aagtatatca agaagtgaaa gaaaaaatcg aagctaaact aaatccatca aatcgaaatc
     6481 gaccaaagaa tcatcaaaaa g
//
