LOCUS       NZ_OU016040             7670 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE53_P46 plasmid pl3, complete
            sequence.
ACCESSION   NZ_OU016040
VERSION     NZ_OU016040.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659977
            Assembly: GCF_907177285.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907177285.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 04:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,267
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 38 of 127
            Pseudo Genes (incomplete)         :: 84 of 127
            Pseudo Genes (internal stop)      :: 23 of 127
            Pseudo Genes (multiple problems)  :: 14 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU016040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE53_P46"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl3"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2018"
     gene            complement(join(7651..7670,1..610))
                     /locus_tag="KKQ98_RS15995"
     CDS             complement(join(7651..7670,1..610))
                     /locus_tag="KKQ98_RS15995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="staphopain proregion domain-containing protein"
                     /protein_id="WP_250133723.1"
                     /translation="MKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPIEEQ
                     AINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNT
                     IKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLW
                     KSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAGELLTT"
                     /name="staphopain proregion domain-containing protein"
     gene            complement(1233..1649)
                     /locus_tag="KKQ98_RS16000"
     CDS             complement(1233..1649)
                     /locus_tag="KKQ98_RS16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368067.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1651..1932)
                     /locus_tag="KKQ98_RS16005"
     CDS             complement(1651..1932)
                     /locus_tag="KKQ98_RS16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368064.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIENDF
                     DQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(2228..2716)
                     /locus_tag="KKQ98_RS16010"
     CDS             complement(2228..2716)
                     /locus_tag="KKQ98_RS16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_060763418.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKEA
                     VTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQQK
                     LLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(2726..3694)
                     /locus_tag="KKQ98_RS16015"
     CDS             complement(2726..3694)
                     /locus_tag="KKQ98_RS16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002368293.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIANFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELLQQLAE
                     LERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
                     /name="replication initiation protein"
     gene            complement(<4382..4527)
                     /locus_tag="KKQ98_RS16395"
                     /pseudo=""
     CDS             complement(<4382..4527)
                     /locus_tag="KKQ98_RS16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(4828..5271)
                     /locus_tag="KKQ98_RS16020"
     CDS             complement(4828..5271)
                     /locus_tag="KKQ98_RS16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223917876.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            complement(5244..5738)
                     /locus_tag="KKQ98_RS16025"
     CDS             complement(5244..5738)
                     /locus_tag="KKQ98_RS16025"
                     /inference="COORDINATES: protein motif:HMM:NF017364.4"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_250133724.1"
                     /translation="MINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYP
                     NLNNSQIESKSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGN
                     YSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITN
                     W"
                     /name="C47 family peptidase"
     gene            5941..6345
                     /gene="tnpA"
                     /locus_tag="KKQ98_RS16030"
     CDS             5941..6345
                     /gene="tnpA"
                     /locus_tag="KKQ98_RS16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            6362..7510
                     /gene="tnpB"
                     /locus_tag="KKQ98_RS16035"
     CDS             6362..7510
                     /gene="tnpB"
                     /locus_tag="KKQ98_RS16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     Typing          complement(2727..3694)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgcaggaaa attttgtttt ttagaaaaat ttttaatttt attttttata tttacggata
       61 taagatcatt tttttctgta agggaagaaa caccattttt ttctgtactt aaaggtgatt
      121 tccaaagtag tttaatttgt ccattatcgg aataataaat gctattatct tgaaaaaata
      181 aggttgtatc tgttatatta ctatctacta gattttgaag ttcttttgct aaaaattgtg
      241 aaatacttcc tgaatattct ccatttgtat tttttttgac ggtataaata tatttaatcg
      301 tattatcagc gtctgaaaat actggaaaga aaatagaact atttgccgta ttttctccat
      361 cgagcatttt aaatggcgaa ccaagagtat agttaatatc agtttcggtg ttattttcca
      421 ttaaaagaat tgaagttatg gagtgttcaa aatcagtttt cgcaaaatta attgcttgtt
      481 cttcaattgg ttgggagttg atattgaaat tagaattttc tgaagcatag gtatttgtaa
      541 ttcctatact aaaaattcca atcatcaagg aaaagaggag acctagataa attttctttt
      601 tgggtttcaa aataatcacg cctttgtgtt aaatttaagt ggtatacacc tataattaaa
      661 atatacaata tgcgataaca aatttttgtt atagttcata aaatattgtt ttgggaacat
      721 aatcgttatt tatagcgtta gttaacataa tgtcgactat gccaagtagc ccctgtacca
      781 accgatccag ccgctcactt tgtgattttt cattggtcta gctaaaaggt acgttcggaa
      841 gcttgatttt ccgctattgt ctgtttacga ttctttgtga atcttaaccg attccttttt
      901 agaaacttgt tatttttcat aaaatatggt aaatttttgg cagcgcttca taagcgcgct
      961 gctttttcaa gaataaacgc tcattatttt ttattggctg ctcgttagag cagtcttttt
     1021 ttatttcaga gtacgttcta tttttttgag ttaccacgct attaaataac cgatatttgg
     1081 agattggggc agcgccccaa aatgcaaaaa gaaggattcg cacaagcgga tttttctttt
     1141 tttgtgcaag ttaccggtaa cttgcaaatc ttgctggaag actgccccct aaaaaagaaa
     1201 ctgagaaata gaaaaaattt tcggtttctt tttcatcttt ttagaaagtt tgttgggtca
     1261 aaatttctag aagtgaatgt ttgattggtt tctttttgac gatttttggg ttgatttcgt
     1321 cgggagggat tgagttgatt ttcaattttt tctttcactt cttgatatac tttagaattt
     1381 gtaccgataa gaacaccagt atcttttaga tataaatcag caaattctgc aatgatttcg
     1441 gacataatag aaatttcttt tcccatcgca tttagttttg cttggttgct ggtagttgtc
     1501 gtattagtat taggttcata actcatacat agaaaacgaa tggcttccga gtagttattg
     1561 atatctggat ttttttcgat gatggaatct aaaacgtcaa tgtgactggg ataaagggta
     1621 acgtgttttt gaacagaagt ttttctcatg ttatattcct ccgataagtg tttcaatgag
     1681 ttggttgttt gtatgaacat agtcgttcaa tgtttcagta ttttttgctt gtatatcgat
     1741 aaatttttcg agtaataaat cgaattgatc ttttttgtaa tgcctaagtg cctggtcaaa
     1801 atcgttttcg attagaactt ttaggtacgc attacggctc cattttttgc cagtttttgt
     1861 tgtgagttct tggcaacgtc tatctatttc tgaaataact tttggatcaa tattccgaac
     1921 taaaatgtcc atagaagaat tcctttcgca tttgtgtata atttatgtat atcatacaaa
     1981 tatagtattt gtatagctgt tttttgccga aacactatac ataagctatc cacaatgacg
     2041 aaaaagtgat agcaaatttt tttcttagaa acgtggtagg cacagcctac ctagcacctt
     2101 tcgtaagaaa gtgataccgc gtttccttat gctctttaaa aaatagcgac gtgagatcat
     2161 gtcgctatgc ttgtaaaagc aaaaaaagaa agtaacccaa caatttttat gaataaaaat
     2221 tgttgggtta ttgccagaat tgccaccatt ttttctcctt gttttgtcct ttttgagttt
     2281 cttcagtaac agaaaggggt tctttttctg aagattcttc tgcataggtt agctggagtt
     2341 gttcgtgaag ttttttgatt tgttgattgg cttgtagggt gagttgttgt tgctggtcga
     2401 gtaatttttg ctgcgtttct aaagctattt gaagtttttc aatttgccta tctttttctt
     2461 caatatgatt attcttagtt tcaatctcct tgagtaggta atcggtaggt aatgaatcgg
     2521 gtaattcact aggtaagtta cccgattcat tacctgataa aacctttgta attgttgtta
     2581 ctgcttcttt tttcaggtag gtaatatttc cttttttgta ggtaaaatta ctaggtaatt
     2641 tcctcagttg atacttcact ttatcttttg aaacacctag aatatctgct aattctttga
     2701 tagttttatc tgtcataaaa accccttatt ctttaaataa gttttggaga ttatctagta
     2761 agttttcttt aaattctggg gtaatatcgg aattgttatc tgctatttgt ttgttaattt
     2821 tcttacttgg ggtattttgt tttctttcta attctgctaa ctgttgtaaa agttctaatt
     2881 ttcgtttttg ttctttgatt ttttcttgta atgaagtagg ttctttttca gattgaacct
     2941 gttgattagc tagttccaaa gctttatttt gttcttcttt ggttggagca tgtttgttaa
     3001 agtcgtctga tttttttatc tcaggtttaa atgtaaattg atagccaatg acaggctttc
     3061 cccgtccttt tccgtatctc ttttggattg tcaaaccttt gaagattgcg gttagttctt
     3121 ggcgaatagg ctttataatt tttttattta tatcactttc tctataactt ttagggatat
     3181 caagtaattc atgaaagtct tcttttgaaa aattggcaat tcctttggtt ctgtattgtt
     3241 ttaataatct aaacatggtt ttggaataac tgctttctaa ttcattgaat tgttggagag
     3301 aatatcgaac ccattcttca agattattaa gtaagggtaa ggctttctca tggacttgta
     3361 tttcagcaaa aggaatatca gcttttccat cgattttaaa ttgattaaat aaaacaaatc
     3421 gttcacgttg caaaccgtca gcacttcttc taccaaaacg taaatccatt aatttgtcat
     3481 acgttgtttc taaatcatct atgaatcgaa catttgcagt tgctttataa ttgcttaaat
     3541 cttttaattg ttcgaatgta aattgtactt ttttatcgcc tacatcacgc attctagata
     3601 ttatagagaa gaataaattc atttctactg acgtaaattt gcgtaaagga atcgtattta
     3661 attcatggtg gtatttcaca atttcatttg ccataaaatc ctccttcaag attaactact
     3721 taacaaaatt ataaagagct ttctaatggg ggtcaacccc ctaattaaag acccctaaaa
     3781 acagacaaaa agacccctaa aaacagacaa aaagacccct aaaaacagac aaaaagaccc
     3841 ctaaaaacag acaaaaagac ccctaaaaga ttctgcacac cttgggagag taaggataca
     3901 gccgacggta aagaggttta aaaagaggtt ataaaagaga ttataaaaga ggggggcaaa
     3961 atttcccgcc taaaattttc ctactttttt tctccaaaca ggttcgcgaa atttcctaaa
     4021 ctgggaatct gctccctacg gtcgctttaa aggtgtgatc gctacgctct cacattaaac
     4081 tgcgcagtcg cctaaaggct cctttgctta aaaacagttc gtaaggagtc cttttagaag
     4141 ctctgagcgc tcatctacgg cgttttaaga caagtctaga taaaatccct tatcttgttt
     4201 ttaaagcgcc ttacaggcgg tcagggcaag ccctaacaac cctaaaacgt tctacagaac
     4261 ctctaaaatt cgttttaagg cgttttaaag tcgctttggt ataattacat accagaacgt
     4321 ttaaaacgcc ttaaaacgca aattagagcc ctatgtatat atcgactaat gttacctaga
     4381 cttttacgga agccggcaca ctcatttgca aagcttcaga gttgcaaatt tggattcgct
     4441 cactcgaaaa ctgatttatt ttgactctga tcaataggtg tattttttag aaggattgct
     4501 agccaaattt tgctttaatt tgctcataag tcccaccctt ttgatacgtc ctatggacgt
     4561 atcgtttgta atgtgaaagt gactataagg tccctttcta caaaacacac taaagggctt
     4621 ggcaagctct atatttgcta gccaaatacc tacgtatttg gtgcaaataa ccgcttgtca
     4681 aaggggcgca agctccccct tgaatccccc tcaaaaccca cgctccacaa tacaaaaaag
     4741 gctagcaaat gctagccact ttttgtaaag tttcgcttgt ttcaccttcg gtgaaattat
     4801 tgttttgtaa tatcaaacaa ctattcatca atcagcttta aaaaattcaa ttggtttagc
     4861 tgtcccttct gtcccatcat tcgttgtcaa aataaatacg agcttttttg gagaatccaa
     4921 actaaaagtg taatctaatt gttttttatt cgagcgcagt gttagtaaat tatttgttgc
     4981 tttgtaaatg aaaacttgtt catttatatt taaattttta tcaatgattt gaattttatg
     5041 attgttctct tttactaaga tggtatattg ttctatattt tttgaagtat ttaaattagt
     5101 gattactaga cgttctgaat gatctttagt ttgccaagtt cctagaaaag gaatcagatc
     5161 agtcttttgt atttcttgtt tttttgaaca actagttaag aaaagaacta acactagacc
     5221 cataactaaa gcaatgagtt tatttaccaa tttgtaatgg tattgtccca agtaaaacca
     5281 cctccaggta caggaattgt aactggtcct gcatttgcac ttattacagt tgggtagtta
     5341 taccaaggat tccacacata gtaagtttga taaccacctt gattgaccca cccaaataat
     5401 gcaagtgcat gacggctgtt actataattt ccataaccag cagttcccat ataaatggca
     5461 tttccttttc ttagttgtgt ttggacctct gaaaggttta gtattcgtgc tattttttta
     5521 ggataaacac cacagtaatt tgcatatttt attatttgat cttgattaag actttttgat
     5581 tcaatttgac tgttgtttaa attaggatag gtataatgca taatggctct agctgaagta
     5641 gcacgtacat ctgttttgtt acataagata gcagcttcta cgtaagcagc gcaccacgga
     5701 aaacttgttt gggtttctgc tatacgccaa ttaatcataa taaagttatc tccggaggag
     5761 acacttttta cacgtttaac agaattaggc agaagtcgcc catcctctat aggtgggtga
     5821 tgaatgccgt tcggtatgag ggttaccgtt ggtaactcga agaccgacaa ctaaggcgta
     5881 ttgccttttt attttgttcc atgttacaat cagtctatac aagaaatgag ttgatataac
     5941 atggaattag atacaaataa tcattcagta tttcttcttt attaccacct tgttttagta
     6001 acgaaatatc gtagacaagt gattgatgat gaaatatctg attatgccaa aactactttt
     6061 gaaagaattt cagaatcgta tcacattact ttagtggagt ggaatcatga taaagaccac
     6121 gttcatatta tgttcaaagc tcaacctaaa acagaattga caaaattcat caatgcctat
     6181 aaaagtgcca gctcaagatt gataaaacgt gactttccaa gagtcaaaca atttctttgg
     6241 aaagaaatgt tctggtctaa aagtttttgt cttctgacaa ctggtggcgc accaattgat
     6301 gtgattaaga agtatatcca aaatcaagga aacaatcata aatagaaagt aggtgaactt
     6361 tatggaacaa ctaaaagcat acaaattcag aatctatcca acagaggaac aagaaatctt
     6421 ctttgccaaa tcttttggtt gtgtccgtaa ggtctacaat ctaatgcttg atgaccgaaa
     6481 aaaggcgtat gaagaagtta aaaatgattc ttctaaaaaa atgacttttc cgacaccagc
     6541 taaatacaag aaagaatttc cgtttttgaa agaaattgat agccttgctt tagccaatgc
     6601 ccaacttaat ttagataaag catacaagaa tttttttcgg gataaatccg ttggatttcc
     6661 acgtttcaaa agtaagaaaa atcctgttca gagttataca acgaataatc aaaatggtac
     6721 agttgctttg attgatagta aattcatcaa agttcctaaa ttgaagtcat tagtaagaat
     6781 taagcttcat agacaaccaa aagggattat caaatctgct acaatatcac gtcattctag
     6841 cggtaaatac tatatttctt tgttgtgtaa agaagaaatt atcgaattac ctaaaactaa
     6901 ttccacaatt ggtattgact taggtattat ggactttgcg attctttctg atggacaaaa
     6961 aattgataat aataaattca cgtccaaaat ggaaaagaaa ctaaagcgtg aacaacgcaa
     7021 attgtctagg cgtgctttat tggctaaaaa taaaggtacc aatctatttg aagctaaaaa
     7081 ttatcaaaaa caaaaacgca aagtagctag attgcatgaa aaagtaatga atcaacgtac
     7141 tgattttctg aataagttga gtacagaaat gatcaaaaat cacgatatta tctgtattga
     7201 agacttaaac acaaaaggta tgttgcgaaa tcataaatta gctaaaagta tttctgatgt
     7261 ttcttggtca aagtttgtga ctaaattaca atacaaggct gactggtatg gacgaaaaat
     7321 catcaaagta gataaatggt ttccgtctag tcagatttgt tcagagtgcg gacataaaga
     7381 tggcaagaaa tctcttgaaa ttcgagaatg gacttgtcct gtttgtcata ctcatcatga
     7441 tcgtgatatc aatgctagta tcaatattct gaccgaaggt ctaagactac attctatggg
     7501 attggcttag atccaatcat caagaaccgt aggaactacg gggatagctt ggtaaataag
     7561 agacaccgct gttagtaaag aaatacgcta tcaagtatgc tctattccca agaagctccc
     7621 acttcaagcg ttaagaaccg tcaggtttag ctaagtggtg agtagttcac
//
