LOCUS       NZ_OU016035             1928 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE67_P60 plasmid pl8, complete
            sequence.
ACCESSION   NZ_OU016035
VERSION     NZ_OU016035.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659991
            Assembly: GCF_907176815.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907176815.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 03:57:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 40 of 130
            Pseudo Genes (incomplete)         :: 84 of 130
            Pseudo Genes (internal stop)      :: 25 of 130
            Pseudo Genes (multiple problems)  :: 15 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU016035.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE67_P60"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl8"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2018"
     gene            complement(<12..194)
                     /locus_tag="KKQ96_RS15580"
                     /pseudo=""
     CDS             complement(<12..194)
                     /locus_tag="KKQ96_RS15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(394..1359)
                     /locus_tag="KKQ96_RS15585"
     CDS             complement(394..1359)
                     /locus_tag="KKQ96_RS15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_213313541.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVGK
                     LNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKFHP
                     DKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPV
                     SFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRG
                     KATDWHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLL
                     KEESQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(395..1161)
                     /name="rep_cluster_1968"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccagacaaac tattgatttt ttggctatca gttaactcac gattattatg agaaatgctt
       61 gtattttttg ttcccttttt gaacgaaata ctaaacccca agttaaaccc ctccttcgcg
      121 ctgtcgctaa taaaaagagt gtaaaccttt ctgttactct ttgacaaaga gtaacatttt
      181 cctaattgtg acatggctgt cacaattcca aatgctctcc gagggtattc gctgtcacaa
      241 gtgacaacga aaaaaggagc cgaaagcgtg tcacgctctc aactcctttt acgctttatt
      301 ttgcttcacg caaaataatc gcttatcgta aactcacgtc attaaacatt gacgtaaatt
      361 tactctcact catctgactt ttcttattcg gtatcacttc tctgtcacgt ccaaacccaa
      421 aagccacgtg tctaattcta ttttcaagtc gtttgcagat tccttaaacg tctctcgtaa
      481 atgattcgtc aactcatcgt tttggctttc ttctttcaac aatttgcgca atctatattt
      541 caaatttctg ctaatatacg cccactctga atgatcttga ttcagccctt ttatcataat
      601 tctatcaaca ggttttacat ctccaggcaa gtaatgagga cttgcaaagc tatctaaact
      661 ttcccgaacc atcgcatgcc aatctgttgc tttgccacgt cttagctgca tttctatacg
      721 ccaccatgtt tcgatttctt ttggtacaat ctgttttttt cgttcctgtt caagcttttt
      781 gttgtacatc cgcacttgtc gctcgctggc acgactaccc cagtacgctg tttctaattt
      841 gccagaacgg ccataaattg gcttaaaaga cacagggtca acgattcgat actgggtaat
      901 aaaatcatca ggaacaccga gaatatcgca agcaatatct gcacgtgaga aatgcgcatc
      961 cagaaacaaa tcatgaatga attgtttcat cgaccccttt aaaaactcgc caattttatt
     1021 tggattaaaa tcaatacgac ctttgtcagg gtgaaatttt aacatttcga tataggcgat
     1081 atcttctcca tattggtcaa ataattgcca accatttgta ccaaccgact tcaaataccc
     1141 ttgattttca ttcaaacgca ttaattgctc aaaatcgaca aacacaactc catttttcgt
     1201 ccaataagct atggtatcat tcagttttcc gacaattgta attcgatcga ttgaccaact
     1261 taattttagg gggtgtacct tatcgtcaac acttaaccgc ctgttagaaa tacggttaag
     1321 tttttcccac tcatttacga ttatgccttt tttactcaaa aatagctcct cctcaacgaa
     1381 aaaagagatt cttggaaaga atttccagag ttcacgtaac tctaaaaatc cacatccaaa
     1441 aatctctagc ttgaagaaac ctaaaaatat tcgtataatg aacttgagtt acggaactag
     1501 gtgaagtagt ttccgtaaag gtcgagagtg cgtcaacact gctcgactat ttttttactc
     1561 ttttttctca ttttctttga ttatagcact cctctagaaa aaataaaaag acctcaggga
     1621 atttcattcc ccgaacccct tgagcctcct cctgccgaaa tagaatggcg ctgccagcat
     1681 ttatttcgtt cagctatcgg ctacacaatc taacacgacc gttccgctac cagtcgcctt
     1741 acaggctcca ttttcgctcc tctaacgtgt ttcaccgatt gtgccgataa ctaaacgaaa
     1801 taaacgctaa aaccgctcag aacgcaaaat atgccatttt agagctattg tagaaaatgt
     1861 agtttgaaat gcaattttct tctttcagct ttttatttcc cacttgtaaa tctgctaatt
     1921 attttcta
//
