LOCUS       NZ_OU016034             2947 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE67_P60 plasmid pl7, complete
            sequence.
ACCESSION   NZ_OU016034
VERSION     NZ_OU016034.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659991
            Assembly: GCF_907176815.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907176815.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 03:57:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 40 of 130
            Pseudo Genes (incomplete)         :: 84 of 130
            Pseudo Genes (internal stop)      :: 25 of 130
            Pseudo Genes (multiple problems)  :: 15 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU016034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE67_P60"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl7"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2018"
     gene            167..1141
                     /locus_tag="KKQ96_RS15560"
     CDS             167..1141
                     /locus_tag="KKQ96_RS15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002337993.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVGT
                     LRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQE
                     GKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYKYRELL
                     KQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1138..1278
                     /locus_tag="KKQ96_RS15565"
     CDS             1138..1278
                     /locus_tag="KKQ96_RS15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_002322683.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAVV
                     N"
                     /name="DeoR family transcriptional regulator"
     gene            complement(1615..2031)
                     /locus_tag="KKQ96_RS15570"
     CDS             complement(1615..2031)
                     /locus_tag="KKQ96_RS15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322684.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKIEK
                     QLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2033..2314)
                     /locus_tag="KKQ96_RS15575"
     CDS             complement(2033..2314)
                     /locus_tag="KKQ96_RS15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347307.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDF
                     DHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
                     /name="hypothetical protein"
     Typing          192..1138
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.899"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcggctaatt acggaaacta ctcacctagt tccgtaactc aaattcatta tacgaatatt
       61 tttaggtttc ttcaagctag agatttttgg atatggattt ttagagttac gtgaactctg
      121 gaaattcttt ccaaaaatct cttttttctt tgaggaggaa caaaatttga gtaaacgtgg
      181 cacgatcata aatgagtggg aaaaacttaa ccgcatatct aacaggcggt taaatgcaca
      241 aactatgtgc acccccttaa aaatgcaatg gtcaattgac cgaattacca ttgttggtac
      301 attaagggaa aatatttatt atcacactcc ccatgatgtg ttgattttag attttgaaca
      361 gcttatgcgt ctaaacgaag gtaacggata tttaaaagcg gttggaaata atggctggca
      421 attactagac caacatgagg aaaatatagc ttatattgag attcttaagt ttcaagaggg
      481 aaaagggcgg attgacttta accccaataa aattagccaa tttctggcgg gctcgatgaa
      541 gaactttatc catgatttat ttttagaacc acatttctca cgtgcagata ttgcttgtga
      601 cattattgac gtgccagatg atttcattac ccaatatcgg gtggttgatc ctgtttcttt
      661 caaaccaatt tacggtcggt ctgggaaact ggaaacagca tattggggaa gtcgtgctag
      721 tgaacgacag attcggatgt ataacaaaaa attggaacaa gaaagaaaac ggaaagttgt
      781 tccgaaagaa atcgatactt ggtggcgact agaattgcaa ttgcgaaggg ggaaagcaac
      841 tgattggcat gcaatggttc atgaaagttt agatagcttt gcaagtcctc actatttgcc
      901 agaagatgtg aaagtaactg acagaatgat gattactgct cttataacgg aacatagtta
      961 ctgggggcaa attcatagaa attcaaaata taaatatcgt gaattgttaa agcaagaaag
     1021 tcaaaatgat gagttgacga atcatttgcg agaaacattt tcggagtcgg cagatgattt
     1081 gaaacgagaa ttagatacat ggcttcaagg acttgatgtg acggaagtag gtgctgaatg
     1141 agtgattcga tagaaaaaat gaccctaaaa aaacttgctg atgaattggg tgtatcgaaa
     1201 aatacggtgc gcaatcaact ggtcaattcg ggatttgacc ttgctcagta taaagaaaaa
     1261 aatgcagtgg tcaattgacc gaaatttatg tctttgggtt ttggggtttt aaaacgaaaa
     1321 aaagggggcg attttcgccc cctttgctct atgtgaaaaa tttgttatac ttattttatc
     1381 cttaaaacat accaatcttt ttcttattta gctgctcgta cgagcagctt tttttacgat
     1441 tatgaattca aatcatgtat ttataatttt tttgttttta tctttttttc tgttatttct
     1501 tctgaaattc gtttttttca ttcaattggt tactatctta ttgtaaatgt tgctattaag
     1561 atggtgatcc tacccaaaaa ataaaccagg aatttaaatc tcctggttta ttttttatct
     1621 ttttagaaaa tttgttgggt cgaaattttt agaagtaaat gtttgattgg tttctttttg
     1681 acgatttttt ggttgatttc gtttggaggg attaagttgt ttttcaattt tttctttcgt
     1741 ttcttgatac actttagaat ttgtgccaat aagaatacct gtatctttta gatataaatc
     1801 agcaaattct gcaatgattt cggacatgat ggaaatttct tttcccatag catttagttt
     1861 ggcttggttg ctggtagtcg tagtattggt atcaggttca taactcatac ataaaaaacg
     1921 aatggcttcc gagtaattgt tgatatctgg atttctttcg ataatggaat ctaaaacgtc
     1981 cacgtgactg ggataaaggg taacgtgttt ttgaacagaa gtttttctca tgttatcttc
     2041 ctccgataag tgtttcaatg agttggttgt ttgtacgaac atattcattc agcgtttcag
     2101 tatttttttc ttgaatagcg ataaattttt cgagtaataa atcgaattga tctttcttgt
     2161 aatgcataag tgcgtggtca aaatcgtttt cgattaggac ttttaagtag cgatttcgac
     2221 tccatttttt gccagtttta tttgtgagtt cttggcaacg tttgtctatt tttgcaataa
     2281 cctttgaatc gatcttccga actaaaatgt ccatagaaaa actcctttcg catttgtgta
     2341 taatttatgt atatcatacg aatatagtat ttgtatagct gttttttgct aaaatactat
     2401 acataagcta gccacgatgg cgaaaaagtg atatcaaatt tttttctgag aaacgtgata
     2461 ggcacagcct atctacccac tttttttaga aagtgggtac acgtttcctt atgctcttta
     2521 taaaatggtg atgtgcgact tttttctgtt cgtaaaaata aaaaaagttt acgaaaatga
     2581 aactaaactg aacgattatg acgggcatgt aatcaagatg aaatgatttt ataagcgcgt
     2641 attttgcgtt ctaagacgtt ttagcgttca tttcgtttag ttatcggcac aatcgttaaa
     2701 acacgtaaga gaagcgagaa tggagcctgt aaggcgactg gtagcgaaac ggcgtgttag
     2761 attgtgcaag ccgagaactg aatgaaatga agagcggaag cgtgaaataa tttcggcagg
     2821 aggaggctca aggggttcgg ggaatgaaat tccctgaggt ctttttattt ttttaagagg
     2881 agtgctataa tcaaagaaaa cgagaaaaaa gagcaaaata aaagccgaac ggtgttggaa
     2941 gcaccct
//
