LOCUS       NZ_OU016030             7670 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE67_P60 plasmid pl3, complete
            sequence.
ACCESSION   NZ_OU016030
VERSION     NZ_OU016030.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659991
            Assembly: GCF_907176815.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907176815.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 03:57:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 40 of 130
            Pseudo Genes (incomplete)         :: 84 of 130
            Pseudo Genes (internal stop)      :: 25 of 130
            Pseudo Genes (multiple problems)  :: 15 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU016030.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE67_P60"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl3"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2018"
     gene            complement(join(7253..7670,1..731))
                     /gene="tnpB"
                     /locus_tag="KKQ96_RS15375"
     CDS             complement(join(7253..7670,1..731))
                     /gene="tnpB"
                     /locus_tag="KKQ96_RS15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(748..1152)
                     /gene="tnpA"
                     /locus_tag="KKQ96_RS15380"
     CDS             complement(748..1152)
                     /gene="tnpA"
                     /locus_tag="KKQ96_RS15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            1355..1849
                     /locus_tag="KKQ96_RS15385"
     CDS             1355..1849
                     /locus_tag="KKQ96_RS15385"
                     /inference="COORDINATES: protein motif:HMM:NF017364.4"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_250133724.1"
                     /translation="MINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYP
                     NLNNSQIESKSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGN
                     YSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITN
                     W"
                     /name="C47 family peptidase"
     gene            1822..2265
                     /locus_tag="KKQ96_RS15390"
     CDS             1822..2265
                     /locus_tag="KKQ96_RS15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223917876.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            2566..>2711
                     /locus_tag="KKQ96_RS15765"
                     /pseudo=""
     CDS             2566..>2711
                     /locus_tag="KKQ96_RS15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            3399..4367
                     /locus_tag="KKQ96_RS15395"
     CDS             3399..4367
                     /locus_tag="KKQ96_RS15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002368293.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIANFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELLQQLAE
                     LERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
                     /name="replication initiation protein"
     gene            4377..4865
                     /locus_tag="KKQ96_RS15400"
     CDS             4377..4865
                     /locus_tag="KKQ96_RS15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_060763418.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKEA
                     VTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQQK
                     LLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            5161..5442
                     /locus_tag="KKQ96_RS15405"
     CDS             5161..5442
                     /locus_tag="KKQ96_RS15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368064.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIENDF
                     DQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            5444..5860
                     /locus_tag="KKQ96_RS15410"
     CDS             5444..5860
                     /locus_tag="KKQ96_RS15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368067.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            6483..7112
                     /locus_tag="KKQ96_RS15415"
     CDS             6483..7112
                     /locus_tag="KKQ96_RS15415"
                     /inference="COORDINATES: protein motif:HMM:NF026081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphopain proregion domain-containing protein"
                     /protein_id="WP_250133723.1"
                     /translation="MKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPIEEQ
                     AINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNT
                     IKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLW
                     KSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAGELLTT"
                     /name="staphopain proregion domain-containing protein"
     Typing          3400..4367
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtttttgat aatttttagc ttcaaataga ttggtacctt tatttttagc caataaagca
       61 cgcctagaca atttgcgttg ttcacgcttt agtttctttt ccattttgga cgtgaattta
      121 ttattatcaa ttttttgtcc atcagaaaga atcgcaaagt ccataatacc taagtcaata
      181 ccaattgtgg aattagtttt aggtaattcg ataatttctt ctttacacaa caaagaaata
      241 tagtatttac cgctagaatg acgtgatatt gtagcagatt tgataatccc ttttggttgt
      301 ctatgaagct taattcttac taatgacttc aatttaggaa ctttgatgaa tttactatca
      361 atcaaagcaa ctgtaccatt ttgattattc gttgtataac tctgaacagg atttttctta
      421 cttttgaaac gtggaaatcc aacggattta tcccgaaaaa aattcttgta tgctttatct
      481 aaattaagtt gggcattggc taaagcaagg ctatcaattt ctttcaaaaa cggaaattct
      541 ttcttgtatt tagctggtgt cggaaaagtc atttttttag aagaatcatt tttaacttct
      601 tcatacgcct tttttcggtc atcaagcatt agattgtaga ccttacggac acaaccaaaa
      661 gatttggcaa agaagatttc ttgttcctct gttggataga ttctgaattt gtatgctttt
      721 agttgttcca taaagttcac ctactttcta tttatgattg tttccttgat tttggatata
      781 cttcttaatc acatcaattg gtgcgccacc agttgtcaga agacaaaaac ttttagacca
      841 gaacatttct ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga
      901 gctggcactt ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa
      961 cataatatga acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc
     1021 tgaaattctt tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct
     1081 acgatatttc gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt
     1141 atctaattcc atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa
     1201 ataaaaaggc aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc
     1261 gaacggcatt catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaacg
     1321 tgtaaaaagt gtctcctccg gagataactt tattatgatt aattggcgta tagcagaaac
     1381 ccaaacaagt tttccgtggt gcgctgctta cgtagaagct gctatcttat gtaacaaaac
     1441 agatgtacgt gctacttcag ctagagccat tatgcattat acctatccta atttaaacaa
     1501 cagtcaaatt gaatcaaaaa gtcttaatca agatcaaata ataaaatatg caaattactg
     1561 tggtgtttat cctaaaaaaa tagcacgaat actaaacctt tcagaggtcc aaacacaact
     1621 aagaaaagga aatgccattt atatgggaac tgctggttat ggaaattata gtaacagccg
     1681 tcatgcactt gcattatttg ggtgggtcaa tcaaggtggt tatcaaactt actatgtgtg
     1741 gaatccttgg tataactacc caactgtaat aagtgcaaat gcaggaccag ttacaattcc
     1801 tgtacctgga ggtggtttta cttgggacaa taccattaca aattggtaaa taaactcatt
     1861 gctttagtta tgggtctagt gttagttctt ttcttaacta gttgttcaaa aaaacaagaa
     1921 atacaaaaga ctgatctgat tccttttcta ggaacttggc aaactaaaga tcattcagaa
     1981 cgtctagtaa tcactaattt aaatacttca aaaaatatag aacaatatac catcttagta
     2041 aaagagaaca atcataaaat tcaaatcatt gataaaaatt taaatataaa tgaacaagtt
     2101 ttcatttaca aagcaacaaa taatttacta acactgcgct cgaataaaaa acaattagat
     2161 tacactttta gtttggattc tccaaaaaag ctcgtattta ttttgacaac gaatgatggg
     2221 acagaaggga cagctaaacc aattgaattt tttaaagctg attgatgaat agttgtttga
     2281 tattacaaaa caataatttc accgaaggtg aaacaagcga aactttacaa aaagtggcta
     2341 gcatttgcta gccttttttg tattgtggag cgtgggtttt gagggggatt caagggggag
     2401 cttgcgcccc tttgacaagc ggttatttgc accaaatacg taggtatttg gctagcaaat
     2461 atagagcttg ccaagccctt tagtgtgttt tgtagaaagg gaccttatag tcactttcac
     2521 attacaaacg atacgtccat aggacgtatc aaaagggtgg gacttatgag caaattaaag
     2581 caaaatttgg ctagcaatcc ttctaaaaaa tacacctatt gatcagagtc aaaataaatc
     2641 agttttcgag tgagcgaatc caaatttgca actctgaagc tttgcaaatg agtgtgccgg
     2701 cttccgtaaa agtctaggta acattagtcg atatatacat agggctctaa tttgcgtttt
     2761 aaggcgtttt aaacgttctg gtatgtaatt ataccaaagc gactttaaaa cgccttaaaa
     2821 cgaattttag aggttctgta gaacgtttta gggttgttag ggcttgccct gaccgcctgt
     2881 aaggcgcttt aaaaacaaga taagggattt tatctagact tgtcttaaaa cgccgtagat
     2941 gagcgctcag agcttctaaa aggactcctt acgaactgtt tttaagcaaa ggagccttta
     3001 ggcgactgcg cagtttaatg tgagagcgta gcgatcacac ctttaaagcg accgtaggga
     3061 gcagattccc agtttaggaa atttcgcgaa cctgtttgga gaaaaaaagt aggaaaattt
     3121 taggcgggaa attttgcccc cctcttttat aatctctttt ataacctctt tttaaacctc
     3181 tttaccgtcg gctgtatcct tactctccca aggtgtgcag aatcttttag gggtcttttt
     3241 gtctgttttt aggggtcttt ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt
     3301 ctttttgtct gtttttaggg gtctttaatt agggggttga cccccattag aaagctcttt
     3361 ataattttgt taagtagtta atcttgaagg aggattttat ggcaaatgaa attgtgaaat
     3421 accaccatga attaaatacg attcctttac gcaaatttac gtcagtagaa atgaatttat
     3481 tcttctctat aatatctaga atgcgtgatg taggcgataa aaaagtacaa tttacattcg
     3541 aacaattaaa agatttaagc aattataaag caactgcaaa tgttcgattc atagatgatt
     3601 tagaaacaac gtatgacaaa ttaatggatt tacgttttgg tagaagaagt gctgacggtt
     3661 tgcaacgtga acgatttgtt ttatttaatc aatttaaaat cgatggaaaa gctgatattc
     3721 cttttgctga aatacaagtc catgagaaag ccttaccctt acttaataat cttgaagaat
     3781 gggttcgata ttctctccaa caattcaatg aattagaaag cagttattcc aaaaccatgt
     3841 ttagattatt aaaacaatac agaaccaaag gaattgccaa tttttcaaaa gaagactttc
     3901 atgaattact tgatatccct aaaagttata gagaaagtga tataaataaa aaaattataa
     3961 agcctattcg ccaagaacta accgcaatct tcaaaggttt gacaatccaa aagagatacg
     4021 gaaaaggacg gggaaagcct gtcattggct atcaatttac atttaaacct gagataaaaa
     4081 aatcagacga ctttaacaaa catgctccaa ccaaagaaga acaaaataaa gctttggaac
     4141 tagctaatca acaggttcaa tctgaaaaag aacctacttc attacaagaa aaaatcaaag
     4201 aacaaaaacg aaaattagaa cttttacaac agttagcaga attagaaaga aaacaaaata
     4261 ccccaagtaa gaaaattaac aaacaaatag cagataacaa ttccgatatt accccagaat
     4321 ttaaagaaaa cttactagat aatctccaaa acttatttaa agaataaggg gtttttatga
     4381 cagataaaac tatcaaagaa ttagcagata ttctaggtgt ttcaaaagat aaagtgaagt
     4441 atcaactgag gaaattacct agtaatttta cctacaaaaa aggaaatatt acctacctga
     4501 aaaaagaagc agtaacaaca attacaaagg ttttatcagg taatgaatcg ggtaacttac
     4561 ctagtgaatt acccgattca ttacctaccg attacctact caaggagatt gaaactaaga
     4621 ataatcatat tgaagaaaaa gataggcaaa ttgaaaaact tcaaatagct ttagaaacgc
     4681 agcaaaaatt actcgaccag caacaacaac tcaccctaca agccaatcaa caaatcaaaa
     4741 aacttcacga acaactccag ctaacctatg cagaagaatc ttcagaaaaa gaaccccttt
     4801 ctgttactga agaaactcaa aaaggacaaa acaaggagaa aaaatggtgg caattctggc
     4861 aataacccaa caatttttat tcataaaaat tgttgggtta ctttcttttt ttgcttttac
     4921 aagcatagcg acatgatctc acgtcgctat tttttaaaga gcataaggaa acgcggtatc
     4981 actttcttac gaaaggtgct aggtaggctg tgcctaccac gtttctaaga aaaaaatttg
     5041 ctatcacttt ttcgtcattg tggatagctt atgtatagtg tttcggcaaa aaacagctat
     5101 acaaatacta tatttgtatg atatacataa attatacaca aatgcgaaag gaattcttct
     5161 atggacattt tagttcggaa tattgatcca aaagttattt cagaaataga tagacgttgc
     5221 caagaactca caacaaaaac tggcaaaaaa tggagccgta atgcgtacct aaaagttcta
     5281 atcgaaaacg attttgacca ggcacttagg cattacaaaa aagatcaatt cgatttatta
     5341 ctcgaaaaat ttatcgatat acaagcaaaa aatactgaaa cattgaacga ctatgttcat
     5401 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
     5461 tcaaaaacac gttacccttt atcccagtca cattgacgtt ttagattcca tcatcgaaaa
     5521 aaatccagat atcaataact actcggaagc cattcgtttt ctatgtatga gttatgaacc
     5581 taatactaat acgacaacta ccagcaacca agcaaaacta aatgcgatgg gaaaagaaat
     5641 ttctattatg tccgaaatca ttgcagaatt tgctgattta tatctaaaag atactggtgt
     5701 tcttatcggt acaaattcta aagtatatca agaagtgaaa gaaaaaattg aaaatcaact
     5761 caatccctcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacattcac
     5821 ttctagaaat tttgacccaa caaactttct aaaaagatga aaaagaaacc gaaaattttt
     5881 tctatttctc agtttctttt ttagggggca gtcttccagc aagatttgca agttaccggt
     5941 aacttgcaca aaaaaagaaa aatccgcttg tgcgaatcct tctttttgca ttttggggcg
     6001 ctgccccaat ctccaaatat cggttattta atagcgtggt aactcaaaaa aatagaacgt
     6061 actctgaaat aaaaaaagac tgctctaacg agcagccaat aaaaaataat gagcgtttat
     6121 tcttgaaaaa gcagcgcgct tatgaagcgc tgccaaaaat ttaccatatt ttatgaaaaa
     6181 taacaagttt ctaaaaagga atcggttaag attcacaaag aatcgtaaac agacaatagc
     6241 ggaaaatcaa gcttccgaac gtacctttta gctagaccaa tgaaaaatca caaagtgagc
     6301 ggctggatcg gttggtacag gggctacttg gcatagtcga cattatgtta actaacgcta
     6361 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
     6421 catattgtat attttaatta taggtgtata ccacttaaat ttaacacaaa ggcgtgatta
     6481 ttttgaaacc caaaaagaaa atttatctag gtctcctctt ttccttgatg attggaattt
     6541 ttagtatagg aattacaaat acctatgctt cagaaaattc taatttcaat atcaactccc
     6601 aaccaattga agaacaagca attaattttg cgaaaactga ttttgaacac tccataactt
     6661 caattctttt aatggaaaat aacaccgaaa ctgatattaa ctatactctt ggttcgccat
     6721 ttaaaatgct cgatggagaa aatacggcaa atagttctat tttctttcca gtattttcag
     6781 acgctgataa tacgattaaa tatatttata ccgtcaaaaa aaatacaaat ggagaatatt
     6841 caggaagtat ttcacaattt ttagcaaaag aacttcaaaa tctagtagat agtaatataa
     6901 cagatacaac cttatttttt caagataata gcatttatta ttccgataat ggacaaatta
     6961 aactactttg gaaatcacct ttaagtacag aaaaaaatgg tgtttcttcc cttacagaaa
     7021 aaaatgatct tatatccgta aatataaaaa ataaaattaa aaatttttct aaaaaacaaa
     7081 attttcctgc gggtgaacta ctcaccactt agctaaacct gacggttctt aacgcttgaa
     7141 gtgggagctt cttgggaata gagcatactt gatagcgtat ttctttacta acagcggtgt
     7201 ctcttattta ccaagctatc cccgtagttc ctacggttct tgatgattgg atctaagcca
     7261 atcccataga atgtagtctt agaccttcgg tcagaatatt gatactagca ttgatatcac
     7321 gatcatgatg agtatgacaa acaggacaag tccattctcg aatttcaaga gatttcttgc
     7381 catctttatg tccgcactct gaacaaatct gactagacgg aaaccattta tctactttga
     7441 tgatttttcg tccataccag tcagccttgt attgtaattt agtcacaaac tttgaccaag
     7501 aaacatcaga aatactttta gctaatttat gatttcgcaa catacctttt gtgtttaagt
     7561 cttcaataca gataatatcg tgatttttga tcatttctgt actcaactta ttcagaaaat
     7621 cagtacgttg attcattact ttttcatgca atctagctac tttgcgtttt
//
