LOCUS       NZ_OU015954             1928 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE5_P5 plasmid pl8, complete
            sequence.
ACCESSION   NZ_OU015954
VERSION     NZ_OU015954.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659929
            Assembly: GCF_907176135.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907176135.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 03:51:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,032
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 40 of 124
            Pseudo Genes (incomplete)         :: 77 of 124
            Pseudo Genes (internal stop)      :: 26 of 124
            Pseudo Genes (multiple problems)  :: 15 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU015954.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE5_P5"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl8"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2015"
     gene            complement(<577..759)
                     /locus_tag="KKQ94_RS15485"
                     /pseudo=""
     CDS             complement(<577..759)
                     /locus_tag="KKQ94_RS15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(959..1924)
                     /locus_tag="KKQ94_RS15490"
     CDS             complement(959..1924)
                     /locus_tag="KKQ94_RS15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_213313541.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVGK
                     LNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKFHP
                     DKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPV
                     SFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRG
                     KATDWHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLL
                     KEESQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(960..1726)
                     /name="rep_cluster_1968"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agctcctcct caacgaaaaa agagattctt ggaaagaatt tccagagttc acgtaactct
       61 aaaaatccac atccaaaaat ctctagcttg aagaaaccta aaaatattcg tataatgaac
      121 ttgagttacg gaactaggtg aagtagtttc cgtaaaggtc gagagtgcgt caacactgct
      181 cgactatttt tttactcttt tttctcattt tctttgatta tagcactcct ctagaaaaaa
      241 taaaaagacc tcagggaatt tcattccccg aaccccttga gcctcctcct gccgaaatag
      301 aatggcgctg ccagcattta tttcgttcag ctatcggcta cacaatctaa cacgaccgtt
      361 ccgctaccag tcgccttaca ggctccattt tcgctcctct aacgtgtttc accgattgtg
      421 ccgataacta aacgaaataa acgctaaaac cgctcagaac gcaaaatatg ccattttaga
      481 gctattgtag aaaatgtagt ttgaaatgca attttcttct ttcagctttt tatttcccac
      541 ttgtaaatct gctaattatt ttctaccaga caaactattg attttttggc tatcagttaa
      601 ctcacgatta ttatgagaaa tgcttgtatt ttttgttccc tttttgaacg aaatactaaa
      661 ccccaagtta aacccctcct tcgcgctgtc gctaataaaa agagtgtaaa cctttctgtt
      721 actctttgac aaagagtaac attttcctaa ttgtgacatg gctgtcacaa ttccaaatgc
      781 tctccgaggg tattcgctgt cacaagtgac aacgaaaaaa ggagccgaaa gcgtgtcacg
      841 ctctcaactc cttttacgct ttattttgct tcacgcaaaa taatcgctta tcgtaaactc
      901 acgtcattaa acattgacgt aaatttactc tcactcatct gacttttctt attcggtatc
      961 acttctctgt cacgtccaaa cccaaaagcc acgtgtctaa ttctattttc aagtcgtttg
     1021 cagattcctt aaacgtctct cgtaaatgat tcgtcaactc atcgttttgg ctttcttctt
     1081 tcaacaattt gcgcaatcta tatttcaaat ttctgctaat atacgcccac tctgaatgat
     1141 cttgattcag cccttttatc ataattctat caacaggttt tacatctcca ggcaagtaat
     1201 gaggacttgc aaagctatct aaactttccc gaaccatcgc atgccaatct gttgctttgc
     1261 cacgtcttag ctgcatttct atacgccacc atgtttcgat ttcttttggt acaatctgtt
     1321 tttttcgttc ctgttcaagc tttttgttgt acatccgcac ttgtcgctcg ctggcacgac
     1381 taccccagta cgctgtttct aatttgccag aacggccata aattggctta aaagacacag
     1441 ggtcaacgat tcgatactgg gtaataaaat catcaggaac accgagaata tcgcaagcaa
     1501 tatctgcacg tgagaaatgc gcatccagaa acaaatcatg aatgaattgt ttcatcgacc
     1561 cctttaaaaa ctcgccaatt ttatttggat taaaatcaat acgacctttg tcagggtgaa
     1621 attttaacat ttcgatatag gcgatatctt ctccatattg gtcaaataat tgccaaccat
     1681 ttgtaccaac cgacttcaaa tacccttgat tttcattcaa acgcattaat tgctcaaaat
     1741 cgacaaacac aactccattt ttcgtccaat aagctatggt atcattcagt tttccgacaa
     1801 ttgtaattcg atcgattgac caacttaatt ttagggggtg taccttatcg tcaacactta
     1861 accgcctgtt agaaatacgg ttaagttttt cccactcatt tacgattatg ccttttttac
     1921 tcaaaaat
//
