LOCUS       NZ_OU015953             2947 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE5_P5 plasmid pl7, complete
            sequence.
ACCESSION   NZ_OU015953
VERSION     NZ_OU015953.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659929
            Assembly: GCF_907176135.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907176135.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 03:51:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,032
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 40 of 124
            Pseudo Genes (incomplete)         :: 77 of 124
            Pseudo Genes (internal stop)      :: 26 of 124
            Pseudo Genes (multiple problems)  :: 15 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU015953.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE5_P5"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl7"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2015"
     gene            complement(72..212)
                     /locus_tag="KKQ94_RS15465"
     CDS             complement(72..212)
                     /locus_tag="KKQ94_RS15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_002322683.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAVV
                     N"
                     /name="DeoR family transcriptional regulator"
     gene            complement(209..1183)
                     /locus_tag="KKQ94_RS15470"
     CDS             complement(209..1183)
                     /locus_tag="KKQ94_RS15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002337993.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVGT
                     LRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQE
                     GKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYKYRELL
                     KQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1983..2264
                     /locus_tag="KKQ94_RS15475"
     CDS             1983..2264
                     /locus_tag="KKQ94_RS15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347307.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDF
                     DHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
                     /name="hypothetical protein"
     gene            2266..2682
                     /locus_tag="KKQ94_RS15480"
     CDS             2266..2682
                     /locus_tag="KKQ94_RS15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322684.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKIEK
                     QLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     Typing          complement(213..1159)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.899"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gagcaaaggg ggcgaaaatc gccccctttt tttcgtttta aaaccccaaa acccaaagac
       61 ataaatttcg gtcaattgac cactgcattt ttttctttat actgagcaag gtcaaatccc
      121 gaattgacca gttgattgcg caccgtattt ttcgatacac ccaattcatc agcaagtttt
      181 tttagggtca ttttttctat cgaatcactc attcagcacc tacttccgtc acatcaagtc
      241 cttgaagcca tgtatctaat tctcgtttca aatcatctgc cgactccgaa aatgtttctc
      301 gcaaatgatt cgtcaactca tcattttgac tttcttgctt taacaattca cgatatttat
      361 attttgaatt tctatgaatt tgcccccagt aactatgttc cgttataaga gcagtaatca
      421 tcattctgtc agttactttc acatcttctg gcaaatagtg aggacttgca aagctatcta
      481 aactttcatg aaccattgca tgccaatcag ttgctttccc ccttcgcaat tgcaattcta
      541 gtcgccacca agtatcgatt tctttcggaa caactttccg ttttctttct tgttccaatt
      601 ttttgttata catccgaatc tgtcgttcac tagcacgact tccccaatat gctgtttcca
      661 gtttcccaga ccgaccgtaa attggtttga aagaaacagg atcaaccacc cgatattggg
      721 taatgaaatc atctggcacg tcaataatgt cacaagcaat atctgcacgt gagaaatgtg
      781 gttctaaaaa taaatcatgg ataaagttct tcatcgagcc cgccagaaat tggctaattt
      841 tattggggtt aaagtcaatc cgcccttttc cctcttgaaa cttaagaatc tcaatataag
      901 ctatattttc ctcatgttgg tctagtaatt gccagccatt atttccaacc gcttttaaat
      961 atccgttacc ttcgtttaga cgcataagct gttcaaaatc taaaatcaac acatcatggg
     1021 gagtgtgata ataaatattt tcccttaatg taccaacaat ggtaattcgg tcaattgacc
     1081 attgcatttt taagggggtg cacatagttt gtgcatttaa ccgcctgtta gatatgcggt
     1141 taagtttttc ccactcattt atgatcgtgc cacgtttact caaattttgt tcctcctcaa
     1201 agaaaaaaga gatttttgga aagaatttcc agagttcacg taactctaaa aatccatatc
     1261 caaaaatctc tagcttgaag aaacctaaaa atattcgtat aatgaatttg agttacggaa
     1321 ctaggtgagt agtttccgta attagccgaa gggtgcttcc aacaccgttc ggcttttatt
     1381 ttgctctttt ttctcgtttt ctttgattat agcactcctc ttaaaaaaat aaaaagacct
     1441 cagggaattt cattccccga accccttgag cctcctcctg ccgaaattat ttcacgcttc
     1501 cgctcttcat ttcattcagt tctcggcttg cacaatctaa cacgccgttt cgctaccagt
     1561 cgccttacag gctccattct cgcttctctt acgtgtttta acgattgtgc cgataactaa
     1621 acgaaatgaa cgctaaaacg tcttagaacg caaaatacgc gcttataaaa tcatttcatc
     1681 ttgattacat gcccgtcata atcgttcagt ttagtttcat tttcgtaaac tttttttatt
     1741 tttacgaaca gaaaaaagtc gcacatcacc attttataaa gagcataagg aaacgtgtac
     1801 ccactttcta aaaaaagtgg gtagataggc tgtgcctatc acgtttctca gaaaaaaatt
     1861 tgatatcact ttttcgccat cgtggctagc ttatgtatag tattttagca aaaaacagct
     1921 atacaaatac tatattcgta tgatatacat aaattataca caaatgcgaa aggagttttt
     1981 ctatggacat tttagttcgg aagatcgatt caaaggttat tgcaaaaata gacaaacgtt
     2041 gccaagaact cacaaataaa actggcaaaa aatggagtcg aaatcgctac ttaaaagtcc
     2101 taatcgaaaa cgattttgac cacgcactta tgcattacaa gaaagatcaa ttcgatttat
     2161 tactcgaaaa atttatcgct attcaagaaa aaaatactga aacgctgaat gaatatgttc
     2221 gtacaaacaa ccaactcatt gaaacactta tcggaggaag ataacatgag aaaaacttct
     2281 gttcaaaaac acgttaccct ttatcccagt cacgtggacg ttttagattc cattatcgaa
     2341 agaaatccag atatcaacaa ttactcggaa gccattcgtt ttttatgtat gagttatgaa
     2401 cctgatacca atactacgac taccagcaac caagccaaac taaatgctat gggaaaagaa
     2461 atttccatca tgtccgaaat cattgcagaa tttgctgatt tatatctaaa agatacaggt
     2521 attcttattg gcacaaattc taaagtgtat caagaaacga aagaaaaaat tgaaaaacaa
     2581 cttaatccct ccaaacgaaa tcaaccaaaa aatcgtcaaa aagaaaccaa tcaaacattt
     2641 acttctaaaa atttcgaccc aacaaatttt ctaaaaagat aaaaaataaa ccaggagatt
     2701 taaattcctg gtttattttt tgggtaggat caccatctta atagcaacat ttacaataag
     2761 atagtaacca attgaatgaa aaaaacgaat ttcagaagaa ataacagaaa aaaagataaa
     2821 aacaaaaaaa ttataaatac atgatttgaa ttcataatcg taaaaaaagc tgctcgtacg
     2881 agcagctaaa taagaaaaag attggtatgt tttaaggata aaataagtat aacaaatttt
     2941 tcacata
//
