LOCUS       NZ_OU015952             4374 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE5_P5 plasmid pl6, complete
            sequence.
ACCESSION   NZ_OU015952
VERSION     NZ_OU015952.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659929
            Assembly: GCF_907176135.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907176135.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 03:51:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,032
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 40 of 124
            Pseudo Genes (incomplete)         :: 77 of 124
            Pseudo Genes (internal stop)      :: 26 of 124
            Pseudo Genes (multiple problems)  :: 15 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU015952.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE5_P5"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl6"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2015"
     gene            complement(399..1805)
                     /locus_tag="KKQ94_RS15435"
     CDS             complement(399..1805)
                     /locus_tag="KKQ94_RS15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_213313540.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEKRHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(2027..2389)
                     /locus_tag="KKQ94_RS15440"
     CDS             complement(2027..2389)
                     /locus_tag="KKQ94_RS15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(2393..2701)
                     /locus_tag="KKQ94_RS15445"
     CDS             complement(2393..2701)
                     /locus_tag="KKQ94_RS15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298419.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(2710..3129)
                     /locus_tag="KKQ94_RS15450"
     CDS             complement(2710..3129)
                     /locus_tag="KKQ94_RS15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(3122..3355)
                     /locus_tag="KKQ94_RS15455"
     CDS             complement(3122..3355)
                     /locus_tag="KKQ94_RS15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(3358..4326)
                     /locus_tag="KKQ94_RS15460"
     CDS             complement(3358..4326)
                     /locus_tag="KKQ94_RS15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002298422.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRG
                     KATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDIL
                     KRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(3366..4243)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.973"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(403..1805)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.88"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagttcacgt aactcttaaa aattcattac caaaaaatct ctagtcttga agaaatctaa
       61 aaatattcgt ataatgatta agaagttacg gagctagtgc gattagtttc cgtaaaggtc
      121 gagaacgttg gtagcgttgc tcgactattt ttttactctt ttttcagttc atttgtttgc
      181 taactcaata atagcacttc tctttagcta tgcaaatatc caaaaaataa aaccgcaccc
      241 tttgaaaagg gcgcccccaa acttcgctaa cgctaggttg gccaaaaaat ggactggcca
      301 ttttttagca aatgtcagct taataggcta gtggtttagc cgtcaactcc ttatcctcgg
      361 cacaaaccgc ctccggtacc gttgactgca catcgtaact accactcgat ctcatcttca
      421 gaacgctcta atttacgctg tgagacgttt tgacgataaa ataaggtttt ctcctgctct
      481 aacacttcta attcctcaga aagccccata tccttttctg caagaccaaa acggatagac
      541 aaacgagtga tcgatttttg aaatgcgttt tttaatcgct ctaaacgctg gcgtaaagag
      601 cgattttctt ctttcagctt tttattttcc acttgtaaat ctgctaattg ttttctacca
      661 gacaaatgac gcttttcttc caatgtagct acatactcat ttaattctgt attttgctca
      721 agcatctgta aatattgctc actcatgtta tcagcatttc tcttggcatt tgttgctctc
      781 gtagctcgtt gatacaaatc aacaaaatca ttcccagata aatgatacga accatcagga
      841 agcctcttga aactttctaa aggaaccata tctttttgga aaatgaccgc catctctttt
      901 tttacattca gcatattttt cttctcttct tcaatcttcg ctctctcaga agccatcaga
      961 gccttatcac gctcgttttc ttctctttgc ctataaatat acctcaactc atcagaaacg
     1021 ctctcacgct cttttacagc cgtttggcgc aattcagaaa tttcactcat gatttctttg
     1081 tattcgtgaa tatctttaat tttgttggta ccgacaatat cccttttcca accgtaatca
     1141 ttcattaatc gttctataga gtcaacttct tgatttctaa aattattgaa aagcactcga
     1201 ccattatctt tatcagcgtg tatcccttgt tgaaccagtg ctttattaaa gctcggctgc
     1261 ttttgaacac cccgtttata accttcagca accggcacaa tattcaagtg caggtgcgga
     1321 cttatttcgt cattatgaat aacagcatta taaacagcca aattagggtt tctctcttga
     1381 aactccttgt aatactgaac caaaatttta ttggcaatca ctcgattttg ttcagcttgt
     1441 tgtttcgata tgccagtaga agaaccatct gctcgatttg tctggaaatc atctaaaatg
     1501 cctatttgca caataaattc tcgttgaggt tccaactttt tatccttgcg cactttcgct
     1561 aaatagtcct cgatttttct atcggctctt ttttgtttcg cattatattt ttctaccact
     1621 tcgccaaaca attcctcata tttatctgct agattcgtct gctctaaata aatattttca
     1681 tccgatttcg tgaagtcaat atgcttatgc caatcattgg ttttctggcg atcagttaac
     1741 tcacgattat tatgagaaat acttgtattc ttcgttcctt ttttaaatga aatactaaac
     1801 cccaagttaa aaccctcctt cgcgctgtcg ctaataaaaa gattgtaaac ctttctgtta
     1861 ctctttgtca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat tccaaatgct
     1921 ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag cgtgtcacgc
     1981 tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat cgtaaattca
     2041 cgtcattaaa tattgacgta aatttactct cactcatctg acttttctta ttcggtatca
     2101 ctccgaataa gccgtcagat tcatcgggat aatctcgttc gcttggcgct gatttttcat
     2161 cagcaagcca ctcattatcc actgattttt cgtgttgctg atcgtcagaa atcgacgata
     2221 gccaccgctc aaaatcagca aaagtttcaa acccaaaacg cttgcgaaga gccttgccca
     2281 ttgcagcatt ttctttctca atcaagcgtt ttttctgttg gcgaagtttc gcttgttctt
     2341 tttttaattt ttcttcttgc gctttaatat ctgctaaagt cattcccata aactacatct
     2401 ccttttcttt ggaattaata acggaaattt ggtttaactg cataacatga ctcattaatt
     2461 tcaaccaaaa ttctttttct gattcatcag ttgtttgcgc taaatgctta tgaacaaatt
     2521 cattagtcaa aactgaagat tttgctagat cagcattgat aaccgagttg agaacatcca
     2581 tcattcgttc aagcatttca ctataattat cttcatccgg caaattcaaa taatcagcta
     2641 accgttcata catcaattca attctatcga ctgtttcttc tctcaaatca attttatcca
     2701 tgtcacacct cacttccaga attgccacca ttttcgattt tttaattctt gattttctag
     2761 taacaactga tcgttttttt gttgctgaag caaagtcaat ttttgttggt cacgaatcat
     2821 ttccgttaat tcagaaatat gcctatcttt ttccgctaat tgttcacgca aaaattcatt
     2881 ttctttttct aacgggaaat cattgcctgc atcattgacc ggtaaatcac cagtcaattg
     2941 accacctttt gaccgataaa ttgaccgcac aacaccaatc aattccttgt ctaacatgat
     3001 aaccccatta tctttatacc gagcaaggtc ataacctgca ttgaccagtt gattgcgcac
     3061 tgtattttta gatataccca actcatcagc aaggtttttt atggtcattt tttctggcaa
     3121 atcagtcatt atcttctccg ttactttcag ctaccccata aacatattta acagcatgct
     3181 tagggataat gattggggaa tcaattttat tatttttcaa aatcaactca tactgcgtta
     3241 catcaagtaa cttaccggac atttttttgc tatccaaaaa aacaacgaca acgttatcac
     3301 ctgttaaatt caccaaccaa tttttcttac caaaatgctt ttgtttttct gccattctta
     3361 ttcctcctcc gttacatcta acccttgaag ccaagtgtct aattcttttt tcaaaacatc
     3421 tgctgattcc gaaaatgttt ctcgtaaatg attcgtcaac tcatcatttt gactttctct
     3481 tttcaaaata tctctcaatt tgtatttcat tctacgctct aaaaaccccc acatttcagg
     3541 gttagcaatc agtccaaaaa tcatcacctt atcatttgtt tttacatcaa gtggtagaaa
     3601 atgagggcta gcaaaactat ttaaactttc atgaaccatt gcatgccaat ctgttgcttt
     3661 cccacgcctt aactgcaatt ccagtcgcca ccaagtttct atttctggtg gcacaatttt
     3721 acgcttggtt tcctgttcca attttttgtt gtatagccga atctggcgtt cgctggcacg
     3781 actgccccaa taagctgttt ccagcttccc agaccgccca taaattggct tgaaagaaac
     3841 aggatcaacc actcgatatt gtgtaataaa ctcatcaggc acatcgataa tatcgcaagc
     3901 aatatccgca cgtgagaaat gtggttctag aaataaatca tgaatgaaat ttttcattga
     3961 actagctaaa aaatgaccaa tcttattcgg gttaaaatca atccgccctt tgccatcttg
     4021 ccatttcaaa atctcaatat aagcaatatt ttcttcgtat tggtctaata attgccagcc
     4081 gttattccca accgctttta aatagccatt gccttcattc acccgcatta attgctcaaa
     4141 atctaaaatc aacacatcat tttgagtgtg ataataaata ttttccttca gttttccaac
     4201 gatagtaatt cggtcaattg accactgcat ttttaagggg gtgtacatat tttgtacatt
     4261 taaccgcctg ttagataggg ctgttaaatt ttcccactca tttacgatta tgccattttt
     4321 actcaaattt tgttcctcct caaagaaaaa gagacttctg gaaagaattt tcaa
//
