LOCUS       NZ_OU015950             6503 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE5_P5 plasmid pl4, complete
            sequence.
ACCESSION   NZ_OU015950
VERSION     NZ_OU015950.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659929
            Assembly: GCF_907176135.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907176135.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 03:51:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,032
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 40 of 124
            Pseudo Genes (incomplete)         :: 77 of 124
            Pseudo Genes (internal stop)      :: 26 of 124
            Pseudo Genes (multiple problems)  :: 15 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU015950.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE5_P5"
                     /isolation_source="Superficial wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl4"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2015"
     gene            complement(32..127)
                     /locus_tag="KKQ94_RS15825"
     CDS             complement(32..127)
                     /locus_tag="KKQ94_RS15825"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106913803.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            780..1499
                     /locus_tag="KKQ94_RS15335"
     CDS             780..1499
                     /locus_tag="KKQ94_RS15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            complement(1636..2052)
                     /locus_tag="KKQ94_RS15340"
     CDS             complement(1636..2052)
                     /locus_tag="KKQ94_RS15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2055..2339)
                     /locus_tag="KKQ94_RS15345"
     CDS             complement(2055..2339)
                     /locus_tag="KKQ94_RS15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            2705..2929
                     /locus_tag="KKQ94_RS15350"
     CDS             2705..2929
                     /locus_tag="KKQ94_RS15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            3052..3231
                     /locus_tag="KKQ94_RS15355"
     CDS             3052..3231
                     /locus_tag="KKQ94_RS15355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806718.1"
                     /translation="MEKDIYKQLTQQIQKEKEKGLLDHLDELELKADEINKNRMSAKEK
                     GIKLKDSSLQSDKS"
                     /name="hypothetical protein"
     gene            3717..3857
                     /locus_tag="KKQ94_RS15360"
     CDS             3717..3857
                     /locus_tag="KKQ94_RS15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016373449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_060806720.1"
                     /translation="MSKKKRLTITLSEQVLEYLDEVAKKKGLSKSALITIALEEYKKGQ
                     K"
                     /name="CopG family transcriptional regulator"
     gene            3923..4663
                     /locus_tag="KKQ94_RS15365"
     CDS             3923..4663
                     /locus_tag="KKQ94_RS15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_060806721.1"
                     /translation="MTVRITKARHFSFLLYPDSIPNDWKEKLESLGISMAVSPLHDMDE
                     KKDKEKWTIDDVVRNGKHYKKPHYHVIYIARNPVTIESIRKKIKRKLGNNSVAHVEIID
                     SIKGAYEYLTHESKDAKAKNKHIYDKKDILYINDFDIDRYITLDESQKRELRKTLTNII
                     DMYGVVNAKELRAFLNWRGTEFGVTNLGDVEDVLSANGSLFRFYFDGNYQNGFRPQYAK
                     RINSETGEIISGVDELISKTEEGE"
                     /name="replication protein"
     gene            4666..4884
                     /locus_tag="KKQ94_RS15370"
     CDS             4666..4884
                     /locus_tag="KKQ94_RS15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002410865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806722.1"
                     /translation="MTKNVNDLFEEYYDNHNLDESRYSHFSKKQLVIEAEYMHDALQNI
                     LKYLDDGGTDLNAIRGEVMDGIYESRI"
                     /name="hypothetical protein"
     gene            complement(4940..5356)
                     /locus_tag="KKQ94_RS15375"
     CDS             complement(4940..5356)
                     /locus_tag="KKQ94_RS15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060811589.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKGTNKTVTSSNFDPTKFLKR"
                     /name="hypothetical protein"
     gene            complement(5359..5643)
                     /locus_tag="KKQ94_RS15380"
     CDS             complement(5359..5643)
                     /locus_tag="KKQ94_RS15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            6009..6233
                     /locus_tag="KKQ94_RS15385"
     CDS             6009..6233
                     /locus_tag="KKQ94_RS15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     Typing          781..1499
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttgtggg ctgagtcgcc ttaattaagt tttacttctt gtttcggtta ttcaaccagt
       61 aactgaacaa agcgcttaaa agcccaacaa gaagggagca aataaagtta gtgaacacga
      121 aacacacctc cttccagtcg caagactgtc ggataggcga caatcaatta tacaataaaa
      181 aagcaaaaat aagcctaagt tttttattgc cttggggttg ttagggcttg ccctgaccgc
      241 ctgtaaggcg ctttagaacg aaggacatag tttttatcga atcttgtctt agaacggctt
      301 agacgagctc tcaggacgcc acaaaggagt ccctaatgaa ttatttttgg cgaaggagcc
      361 taaaggcgac tgctgcaagt aaaatgtgag agcgtagcga tcactccttc ttacagcgac
      421 catagggagc tagattggca gtttaatatc atgcgcgaac ctgtttattt taaaaagtgg
      481 gtgatttttt ggcgggaaat tttgctcgcg cgcgcgcgtt tattttattt attttttaaa
      541 gattttttag aacccttttt agggggcagt tgagccttac tctcccaacg gatacagaag
      601 ggtactaaca actttttatc tgagtactaa caacttttta tctgagtact aacaactttt
      661 tatctgagta ctaacaactt tttatctgag tactaatatc cttttgtata ataacaacca
      721 acctatttac gagttggttg ttaaaggtta ttatttattt gtaagggggg aagaaaggaa
      781 tggatgaaaa aacggaatta acagtcaaat atcaaaatga attgaattta gtaccgctga
      841 aaaatttcaa tgctaagcag atggatttat tttttgcatt atgcgctcgt atgaaagatc
      901 gtggtttgaa aaaagttaga ttttctttcg aggaattaaa agagttaagt gattataaga
      961 tgactgctac aaaagccttt gtcggtgatt tagaaaagct ctataaagat atgcttaatc
     1021 ttacttaccg tactgaagat gaggattcta ttcgctactt tgttttgttt actggttttg
     1081 aaatcaataa aacaaaaaag tttgttgaag tcagtgttaa tccggaccta gaatatatta
     1141 ttaatggttt aacagctgaa tttagtagat ttgaattatc tgctttcacg tcgttgcgtt
     1201 ctacgtatgc aaaaactcta ttccgactgc ttatgcagta ccgttctact ggttattatg
     1261 tagttcgtat tgaagagttt aggagcttaa tggatatccc agaatactat caaatgggta
     1321 acattgatca aaaagtatta aatcctgcat tacgagaact aaaaaaatac ttcaatgatc
     1381 ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat cgccaaatta gaatttactt
     1441 tttcaagcct aacgtcagat ttgccaaaga tttctctgcg taactggtta gaagaataaa
     1501 taaaaaagaa ctcgcccccc ttacattaag caagtagaca agtcccttac aaccgactaa
     1561 gattgctagt acattctagc atattgtatg gggcttctaa ataaaaaaca gataggaata
     1621 actcctatct gttttttatc gttttagaaa atttgttggg tcgaaatttc tagaagtgac
     1681 tgtttgactg gtttcttttt gatgattctt tggtcgattt cgatttgatg gatttagttt
     1741 agcttcgatt ttttctttca cttcttgata tactttagaa tttgtgccaa taagaacgcc
     1801 tgtatctttt agatataaat cagcaaattc tgcgatgatt tcagtcatga tggaaatttc
     1861 ttttcccata gcatttagtt ttgcctgatt gctggtagtg gtcgtgttgg tatcgggttc
     1921 ataactcata cataaaaagc gaatggcttc cgagtagtta ttgatatctg gatttttttc
     1981 gatgatggaa tctaaaacgt caacatgact agggtacaaa gtaacatgtt tttggacaga
     2041 agtttttctc atggttatat cattcctccg ataagcattt caatgatttg attatttgtg
     2101 tagacatact cgcttaatat ttgagtattt tctgtttgga tagcaataaa tttatcgagc
     2161 aataaatcga attggtcttt tttgtaagtc gtaagttgac ggtcaaaatc actttcgatt
     2221 aaaactttta gatatttatt tcgactccat ttttctcctg ttttattcgt gagttcttgg
     2281 caacgtttgt ctattgttgc aacaaacttt ggatcaatat tccgaactaa aatgtccata
     2341 aaaaatctcc ttttgcaatt atgtataatt tatgtatatc atacaaatac tatatttgta
     2401 tagctgtttt ttgccaaaat actatacata agctatccac gatggcgaaa aagtgatatc
     2461 aaattttttt ctgagaaacg tgataggcac agcctatcta gcacctttcg taagaaagtg
     2521 ataccacgtt tccttatgct ctttaaaatt cgggggggag tgctgcacgt gtaatcgata
     2581 accaaattta aaaatgcacg atcaaaaatt tatttagttt ttcaaacgac attttttttg
     2641 agagtataga cggcaaagaa gtgattttat ttctagaaaa attgtgagac tggagaaaga
     2701 aaaaatggaa aaatttcctt acgaatattt tcaaaactta gaaaaattag agcaagcaat
     2761 tcaagcaatc aagttcgatt taacagacga aaaattgttg gaatataaaa atatgacacc
     2821 cgaaagaaaa cgatcgttgg aaaaattaat gcaactagtg aacgaagacg gcggaatttc
     2881 agacgcagct tttgacgcat ggagcgaatt tcatggagaa tataattaag tgggtttaaa
     2941 tggctctaaa atcgcttcta agagtttttt agcttggacc aatgaaatac accaaaaact
     3001 aaataaaacg tcttaaatcg aataataata gcttttaagg aggtgatggg gatggaaaaa
     3061 gatatttata aacaattgac ccaacaaatt caaaaggaaa aagaaaaagg cttgctagat
     3121 catttagatg aattagaact aaaagctgat gaaatcaata aaaacagaat gtcagcaaaa
     3181 gaaaaaggca taaaacttaa agattcgtcg ttgcaaagcg ataaaagcta gataacgtgc
     3241 ggtcaacggt aaatcgaatt agagacgctt agagaggcta ataagccctc ttagcgtctc
     3301 tttgaggctt taaggagttg acggctataa acaaaaccca aaagctggaa acacaagaag
     3361 gtgaccatag ggaacgttct tgacgcctta acggcgagtg gaaagttttt ggggtgcctt
     3421 ttttcaaaaa ggtacggtct tgatttgccg agcggtagcg ctggaaaatt tttggaaaaa
     3481 atttggaatt tggaaaatct ggggggctac tacgaccccc catagtgtgc cgagtgccaa
     3541 tttttgtgcc aaaaagtgct ttatccttac tgtaccaatg gatagagcct tttttttcga
     3601 ccgccatttg accgccgttt cgaccgccgt ttttaaaaaa atacgctttt agttgcattt
     3661 aatgcgtaat atgtggtatt ctttttaaga agataaataa tgaaaaggag aatgacatga
     3721 gtaagaagaa acgtttgaca ataacgttgt cagaacaagt tttagaatat ctagacgaag
     3781 tagctaaaaa aaagggttta tccaaatctg cattaataac aatagcgtta gaagaataca
     3841 agaaagggca aaaatgagca taaaaaaaga gcgctcccga ctaaaagacc gctcactcat
     3901 taattgataa ggtgattata tcatgactgt tagaattact aaagctagac attttagttt
     3961 tttgttatat cctgattcga ttccaaatga ttggaaggaa aaattagaga gtttaggtat
     4021 ttcaatggct gttagtcctt tacacgatat ggatgaaaaa aaggataaag aaaagtggac
     4081 aattgatgat gttgttagaa atggtaagca ctataaaaaa ccgcattatc atgttatata
     4141 tattgctaga aatccagtaa cgatagaaag tattcgaaaa aaaatcaaga gaaaattggg
     4201 aaataattcg gttgctcatg ttgaaataat tgattccatt aagggtgctt atgagtattt
     4261 gactcatgag tcaaaagacg cgaaagcgaa gaacaaacat atttacgata aaaaagatat
     4321 tttgtatatt aatgattttg atattgatcg ttatatcacc cttgatgaaa gccaaaaacg
     4381 agagttaaga aaaactttga caaatattat tgatatgtac ggtgtggtta atgcgaaaga
     4441 acttagagca tttcttaatt ggagaggtac agaatttggt gtaaccaact taggagatgt
     4501 agaagatgtt ttgagtgcaa atggtagtct gtttagattt tattttgatg ggaactatca
     4561 aaatgggttt agaccacaat atgcaaaaag aataaattct gaaactgggg aaattataag
     4621 tggagttgac gaattaataa gtaaaactga agaaggagaa taagaatgac aaaaaacgta
     4681 aacgatttgt ttgaggaata ttatgataat cataatttag atgaaagtcg gtatagtcat
     4741 tttagtaaaa agcaattggt gattgaagct gagtatatgc atgatgcttt gcaaaatatt
     4801 ttgaaatatc tcgacgacgg tggaacggat ttgaatgcga ttcgtggaga agtcatggac
     4861 ggcatatatg aaagtaggat atgaaaaaat agaatattgg gaaataaaaa acagatagga
     4921 ataactccta tctgtttttt tatcgtttta gaaatttagt tgggtcgaaa ttgctagaag
     4981 tgactgtttt gtttgttcct ttttgatgat tctttggtcg atttcgattt gatggattta
     5041 gtttagcttc gattttttct ttcacttctt gatatacttt agaatttgtg ccaataagaa
     5101 cgcctgtatc ttttagatat aaatcagcaa attctgcgat gatttcagtc atgatggaaa
     5161 tttcttttcc catagcattt agttttgcct gattgctggt agtggtcgtg ttggtatcgg
     5221 gttcataact catacataaa aagcgaatgg cttccgagta gttattgata tctggatttt
     5281 tttcgatgat ggaatctaaa acgtcaacat gactagggta caaagtaaca tgtttttgga
     5341 cagaagtttt tctcatggtt atatcattcc tccgataagc atttcaatga tttgattatt
     5401 tgtgtagaca tactcgctta atatttgagt attttctgtt tggatagcaa taaatttatc
     5461 gagcaataaa tcgaattggt cttttttgta agtcgtaagt tgacggtcaa aatcactttc
     5521 gattaaaact tttagatatt tatttcgact ccatttttct cctgttttat tcgtgagttc
     5581 ttggcaacgt ttgtctattg ttgcaacaaa ctttggatca atattccgaa ctaaaatgtc
     5641 cataaaaaat ctccttttgc aattatgtat aatttatgta tatcatacaa atactatatt
     5701 tgtatagctg ttttttgcca aaatactata cataagctat ccacgatggc gaaaaagtga
     5761 tatcaaattt ttttctgaga aacgtgatag gcacagccta tctagcacct ttcgtaagaa
     5821 agtgatacca cgtttcctta tgctctttaa aattcggggg ggagtgctgc acgtgtaatc
     5881 gataaccaaa tttaaaaatg cacgatcaaa aatttattta gtttttcaaa cgacattttt
     5941 tttgagagta tagacggcaa agaagtgatt ttatttctag aaaaattgtg agactggaga
     6001 aagaaaaaat ggaaaaattt ccttacgaat attttcaaaa cttagaaaaa ttagagcaag
     6061 caattcaagc aatcaagttc gatttaacag acgaaaaatt gttggaatat aaaaatatga
     6121 cacccgaaag aaaacgatcg ttggaaaaat taatgcaact agtgaacgaa gacggcggaa
     6181 tttcagacgc agcttttgac gcatggagcg aatttcatgg agaatataat taagtgggtt
     6241 taaatggctc taaaatcgct tctaagagtt ttttagcttg gaccaatgaa atacaccaaa
     6301 aactaaataa aacgtcttaa atcgaaaaat acgagctctc agagcgactt tatttgtaaa
     6361 tgttgcatat aagaaataat tacggatttt agacttttgt aggatatttt tgaaataaat
     6421 atttatttct gttataattt gattgcaaat aagtgaacac gaaacacacg aaagccccag
     6481 agaggtcgga ttctctgggg ctt
//
