LOCUS       NZ_OU015715             3221 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Enterococcus faecium isolate USZ_VRE32_P32 plasmid pl6, complete
            sequence.
ACCESSION   NZ_OU015715
VERSION     NZ_OU015715.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8659956
            Assembly: GCF_907165365.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Boumasmoud,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2021) UZH, Raemistrasse 100, Switzerland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_907165365.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 03:49:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,079
            CDSs (with protein)               :: 3,079
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 37 of 107
            Pseudo Genes (incomplete)         :: 69 of 107
            Pseudo Genes (internal stop)      :: 21 of 107
            Pseudo Genes (multiple problems)  :: 16 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU015715.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="USZ_VRE32_P32"
                     /isolation_source="Inguinal/Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pl6"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.4 N 8.5 E"
                     /collection_date="2017"
     gene            complement(join(3163..3221,1..358))
                     /locus_tag="KKQ93_RS16230"
     CDS             complement(join(3163..3221,1..358))
                     /locus_tag="KKQ93_RS16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060807318.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDINTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(361..645)
                     /locus_tag="KKQ93_RS16235"
     CDS             complement(361..645)
                     /locus_tag="KKQ93_RS16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            1011..1235
                     /locus_tag="KKQ93_RS16240"
     CDS             1011..1235
                     /locus_tag="KKQ93_RS16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            complement(1537..1632)
                     /locus_tag="KKQ93_RS16610"
     CDS             complement(1537..1632)
                     /locus_tag="KKQ93_RS16610"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106913803.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            2307..3026
                     /locus_tag="KKQ93_RS16250"
     CDS             2307..3026
                     /locus_tag="KKQ93_RS16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     Typing          2308..3026
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctttttgatg attctttggt cgatttcgat ttgatggatt tagtttagct tcgatttttt
       61 ctttcacttc ttgatatact ttagaatttg tgccaataag aacgcctgta tcttttagat
      121 ataaatcagc gaattctgcg atgatttcag tcatgatgga aatttctttt cccatagcat
      181 ttagttttgc ctgattgctg gtagtggtcg tgttgatatc gggttcataa ctcatacata
      241 aaaagcgaat ggcttccgag tagttattga tatctggatt tttttcgatg atggaatcta
      301 aaacgtcaac atgactaggg tacaaagtaa catgtttttg gacagaagtt tttctcatgg
      361 ttatatcatt cctccgataa gcatttcaat gatttgatta tttgtgtaga catactcgct
      421 taatatttga gtattttctg tttggatagc aataaattta tcgagcaata aatcgaattg
      481 gtcttttttg taagtcgtaa gttgacggtc aaaatcactt tcgattaaaa cttttagata
      541 tttatttcga ctccattttt ctcctgtttt attcgtgagt tcttggcaac gtttgtctat
      601 tgttgcaaca aactttggat caatattccg aactaaaatg tccataaaaa atctcctttt
      661 gcaattatgt ataatttatg tataccatac aaatactata tttgtatagc tgttttttgc
      721 caaaatgcta tacataagct acccacgatg gcgaaaaagt gatatcaaat ttttttctga
      781 gaaacgtgat aggcacagcc tatctaccca ctttttttag aaagtgggta cacgtttcct
      841 tatgctcttt aaaattcggg ggggagtgct gcacgtgtaa tcgataacca aatttaaaaa
      901 tgcacgatca aaaatttatt tagtttttca aacgacattt tttttgagag tatagacggc
      961 aaagaagtga ttttatttct agaaaaattg tgagactgga gaaagaaaaa atggaaaaat
     1021 ttccttacga atattttcaa aacttagaaa aattagagca agcaattcaa gcaatcaagt
     1081 tcgatttaac agacgaaaaa ttgttggaat ataaaaatat gacacccgaa agaaaacgat
     1141 cgttggaaaa attaatgcaa ctagtgaacg aagacggcgg aatttcagac gcagcttttg
     1201 acgcatggag cgaatttcat ggagaatata attaagtggg tttaaatggc tctaaaatcg
     1261 cttctaagag ttttttagct tggaccaatg aaatacacca aaaactaaat aaaacgtctt
     1321 aaatcgaaaa atacgagctc tcagagcgac tttatttgta aatgttgcat ataagaaata
     1381 attacggatt ttagactttt gtaggatatt tttgaaataa atatttattt ctgttataat
     1441 ttgattgcaa ataagtgaac acgaaacaca cgaaagcccc agagaggtcg gattctctgg
     1501 ggcttttttt gtgggctgag tcgccttaat taagttttac ttcttgtttc ggttattcaa
     1561 ccagtaactg aacaaagcgc ttaaaagccc aacaagaagg gagcaaataa agttagtgaa
     1621 cacgaaacac acctccttcc agtcgcaaga ctgtcggata ggcgacaatc aattatacaa
     1681 taaaaaagca aaaataagcc taagtttttt attgccttgg ggttgttagg gcttgccctg
     1741 accgcctgta aggcgcttta gaacgaagga catagttttt atcgaatctt gtcttagaac
     1801 ggcttagacg agctctcagg acgccacaaa ggagtcccta atgaattatt tttggcgaag
     1861 gagcctaaag gcgactgctg caagtaaaat gtgagagcgt agcgatcact ccttcttaca
     1921 gcgaccatag ggagctagat tggcagttta atatcatgcg cgaacctgtt tattttaaaa
     1981 agtgggtgat tttttggcgg gaaattttgc tcgcgcgcgc gcgtttattt tatttatttt
     2041 ttaaagattt tttagaaccc tttttagggg gcagttgagc cttactctcc caacggatac
     2101 agaagggtac taacaacttt ttatctgagt actaacaact ttttatctga gtactaacaa
     2161 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2221 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2281 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2341 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2401 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2461 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2521 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2581 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2641 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2701 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2761 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2821 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     2881 gagaactaaa aaaatacttc aatgatctta aagtcacaaa aataaaagca aaaaagggca
     2941 ataaaatcgc caaattagaa tttacttttt caagcctaac gtcagatttg ccaaagattt
     3001 ctctgcgtaa ctggttagaa gaataaataa aaaagaactc gcccccctta cattaagcaa
     3061 gtagacaagt cccttacaac cgactaagat tgctagtaca ttctagcata ttgtatgggg
     3121 cttctaaata aaaaacagat aggaataact cctatctgtt ttttatcgtt ttagaaaatt
     3181 tgttgggtcg aaatttctag aagtgactgt ttgactggtt t
//
