LOCUS       NZ_OU015349            10069 bp    DNA     circular CON 14-SEP-2023
DEFINITION  Enterococcus faecium strain AUS2001 isolate AUS2001 plasmid P04,
            complete sequence.
ACCESSION   NZ_OU015349
VERSION     NZ_OU015349.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8520472
            Assembly: GCF_907163255.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Baines L,S., Ingle J,D., Sherry,N., Gorrie L,C. and Goncalves,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2021) THE UNIVERSITY OF MELBOURNE, 792 Elizabeth
            Street, Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/14/2023 04:27:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,073
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,870
            CDSs (with protein)               :: 2,870
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 41 of 109
            Pseudo Genes (incomplete)         :: 68 of 109
            Pseudo Genes (internal stop)      :: 22 of 109
            Pseudo Genes (multiple problems)  :: 18 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OU015349.1.
            Complete genome of vancomycin susceptible Enterococcus faecium
            AUS2001.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AUS2001"
                     /isolate="AUS2001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="P04"
                     /geo_loc_name="New Zealand"
                     /collection_date="2012"
     gene            1..933
                     /locus_tag="K3836_RS15160"
     CDS             1..933
                     /locus_tag="K3836_RS15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="K3836_RS15165"
     CDS             950..1420
                     /locus_tag="K3836_RS15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1821
                     /locus_tag="K3836_RS15170"
     CDS             1504..1821
                     /locus_tag="K3836_RS15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002302541.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRILL
                     F"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2111..2806
                     /locus_tag="K3836_RS15175"
     CDS             2111..2806
                     /locus_tag="K3836_RS15175"
                     /inference="COORDINATES: protein motif:HMM:NF013279.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_230079062.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLVNYSPLS"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(2943..4091)
                     /gene="tnpB"
                     /locus_tag="K3836_RS15180"
     CDS             complement(2943..4091)
                     /gene="tnpB"
                     /locus_tag="K3836_RS15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(4108..4512)
                     /gene="tnpA"
                     /locus_tag="K3836_RS15185"
     CDS             complement(4108..4512)
                     /gene="tnpA"
                     /locus_tag="K3836_RS15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            5195..6295
                     /locus_tag="K3836_RS15190"
     CDS             5195..6295
                     /locus_tag="K3836_RS15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_222717721.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFT
                     EKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            6268..6711
                     /locus_tag="K3836_RS15195"
     CDS             6268..6711
                     /locus_tag="K3836_RS15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            7013..7393
                     /locus_tag="K3836_RS15200"
     CDS             7013..7393
                     /locus_tag="K3836_RS15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002348702.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7375..8289
                     /locus_tag="K3836_RS15205"
     CDS             7375..8289
                     /locus_tag="K3836_RS15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002348701.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8249..8527
                     /locus_tag="K3836_RS15535"
     CDS             8249..8527
                     /locus_tag="K3836_RS15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            8529..9218
                     /locus_tag="K3836_RS15210"
     CDS             8529..9218
                     /locus_tag="K3836_RS15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347611.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7376..8286
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatt atggtaaaaa atcatacaaa
     1741 aacactaata ctattatttc attttgtaat ttttttaagt ttagttttca ccttaattca
     1801 gtccggattc ttttatttta gccattcaat tgccacttgg ttattaatat tttttctaag
     1861 tttactatta ataattaata cattaataat taatacttta attattatct ttaataaaca
     1921 tagaaactta tttcttcaca gcatatttcc aattgtttta ttaatttttc tatactttat
     1981 ataaaaagat agaactattt agtaagataa aaaagtagct aattgtaatt ttcgtctaaa
     2041 actcaaaact agtaaaaata actttattgt gttattttta tttataaatt atgaaaaagg
     2101 acctttcgaa atgaaaaata atagaatcta caaaaatcag ttgaattatg ggcttttatt
     2161 gccagttttt ctgctatgta tcattggatt actttcttta tatgttgctt tatatcacga
     2221 ctcttacact aattcaaaaa tcacaaaaga attaattaaa caagtattgt ggtatagtat
     2281 tgggttcact tcagcaataa ctatttattt ttttaatcct aaaattttgt ggaaattaac
     2341 tcctatattt tacggattat gcatcataat gttattactt ctcttgaaat tctataatcc
     2401 acaattagca gtcttaactg gagcaaaaaa ttggttttat attgctggtt tttcgtttca
     2461 acctactgag ttagtcaaaa taggatacac ccttatgcta gctaaaataa ccgtcaatta
     2521 tcagcaaaaa tctattacta gtaatacgaa agatgattgg atttatttac ttaaaacttt
     2581 tattgctact cttccaatag gaatattatt attttttcag aacgatctag gaacaatgtt
     2641 agtcttcatt gttatttata tttttatttt gtttacgtct aacataaata taaaaataat
     2701 tgccccaata atcgccattg gtgttgtttt atccttaata tttttatatt tggttatatc
     2761 agatacagga agagatttct tagtgaacta ctcaccactt agctaaacct gacggttctt
     2821 aacgcttgaa gtgggagctt cttgggaata gagcatactt gatagcgtat ttctttacta
     2881 acagcggtgt ctcttattta ccaagctatc cccgtagttc ctacggttct tgatgattgg
     2941 atctaagcca atcccataga atgtagtctt agaccttcgg tcagaatatt gatactagca
     3001 ttgatatcac gatcatgatg agtatgacaa acaggacaag tccattctcg aatttcaaga
     3061 gatttcttgc catctttatg tccgcactct gaacaaatct gactagacgg aaaccattta
     3121 tctactttga tgatttttcg tccataccag tcagccttgt attgtaattt agtcacaaac
     3181 tttgaccaag aaacatcaga aatactttta gctaatttat gatttcgcaa catacctttt
     3241 gtgtttaagt cttcaataca gataatatcg tgatttttga tcatttctgt actcaactta
     3301 ttcagaaaat cagtacgttg attcattact ttttcatgca atctagctac tttgcgtttt
     3361 tgtttttgat aatttttagc ttcaaataga ttggtacctt tatttttagc caataaagca
     3421 cgcctagaca atttgcgttg ttcacgcttt agtttctttt ccattttgga cgtgaattta
     3481 ttattatcaa ttttttgtcc atcagaaaga atcgcaaagt ccataatacc taagtcaata
     3541 ccaattgtgg aattagtttt aggtaattcg ataatttctt ctttacacaa caaagaaata
     3601 tagtatttac cgctagaatg acgtgatatt gtagcggatt tgataatccc ttttggttgt
     3661 ctatgaagct taattcttac taatgacttc aatttaggaa ctttgatgaa tttactatca
     3721 atcaaagcaa ctgtaccatt ttgattattc gttgtataac tctgaacagg atttttctta
     3781 cttttgaaac gtggaaatcc aacggattta tcccgaaaaa aattcttgta tgctttatct
     3841 aaattaagtt gggcattggc taaagcaagg ctatcaattt ctttcaaaaa cggaaattct
     3901 ttcttgtatt tagctggtgt cggaaaagtc atttttttag aagaatcatt tttaacttct
     3961 tcatacgcct tttttcggtc atcaagcatt agattgtaga ccttacggac acaaccaaaa
     4021 gatttggcaa agaagatttc ttgttcctct gttggataga ttctgaattt gtatgctttt
     4081 agttgttcca taaagttcac ctactttcta tttatgattg tttccttgat tttggatata
     4141 cttcttaatc acatcaattg gtgcgccacc agttgtcaga agacaaaaac ttttagacca
     4201 gaacatttct ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga
     4261 gctggcactt ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa
     4321 cataatatga acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc
     4381 tgaaattctt tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct
     4441 acgatatttc gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt
     4501 atctaattcc atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa
     4561 ataaaaaggc aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc
     4621 gaacggcatt catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatt
     4681 caaattatta caacagtcct aacgaagaat tgtttgtaaa acaaacggat aaataataaa
     4741 aaagactgct ctttcgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca
     4801 acgcgcttat gaagcgctac caaaaattta tcatatttta tgaaaaataa caccccagta
     4861 aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caactgcaga aaatcaagcc
     4921 ttaaaaagag cttttgacct agaccaatga aaaatcacaa agtgagcccc tgtatccctt
     4981 ggtagagagg ctacttggca tagtcgacat tatgttaact aacgctataa ataacgatta
     5041 tgttcccaaa acaatatttt atgaactata acaaaaattt gttatcgcat attgtatatt
     5101 ttaattatag gtgtatacca cttaaattta acacaaaggc gtgattattt tgaaacccaa
     5161 aaagaaaatt tatctaggtc tcctcttttc cttgatgatt ggaattttta gtataggaat
     5221 tacaaatacc tatgcttcag aaaattctaa tttcaatatc aactcccaac caattgaaga
     5281 acaagcaatt aattttgcga aaactgattt tgaacactcc ataacttcaa ttcttttaat
     5341 ggaaaataac accgaaactg atattaacta tactcttggt tcgccattta aaatgctcga
     5401 tggagaaaat acggcaaata gttctatttt ctttccagta ttttcagacg ctgataatac
     5461 gattaaatat atttataccg tcaaaaaaaa tacaaatgga gaatattcag gaagtatttc
     5521 acaattttta gcaaaagaac ttcaaaatct agtagatagt aatataacag atacaacctt
     5581 attttttcaa gataatagca tttattattc cgataatgga caaattaaac tactttggaa
     5641 atcaccttta agtacagaaa aaaatggtgt ttcttccttt acagaaaaaa atgatcttat
     5701 atccgtaaat ataaaaaata aaattaaaaa tttctctaaa aaacaaaatt ttcctgcggt
     5761 taaacgtgta aaaagtgtct cctccggaga taactttatt atgattaatt ggcgtatagc
     5821 agaaacccaa acaagttttc cgtggtgtgc tgcttacgta gaagctgcta tcttatgtaa
     5881 caaaacagat gtacatgcta cttcagctag agccattatg cattatacct atcctaattt
     5941 aaacaacagt caaattgaat caaaaagtct taatcaagat caaataataa aatatgcaaa
     6001 ttactgtggt gtttatccta aaaaagtagc acgaatacta aacctttcag aggtccaaac
     6061 acaactaaga aaaggaaatg ccatttatat gggaactgct ggttatggaa attatagtaa
     6121 cagccgtcat gcacttgcat tatttgggtg ggtcaatcaa ggtggttatc aaacttacta
     6181 tgtgtggaat ccttggtata actacccaac tgtaataagt gcaaatgcag gaccagttac
     6241 aattcctgta cctggaggtg gttttacttg ggacaatacc attacaaatt ggtaaataaa
     6301 cccattgctt tagttatggg tctagtgtta gttcttttct taactagttg ttcaaaaaaa
     6361 caagaaatac aaaagactga tctgattcct tttctaggaa cttggcaaac taaagatcat
     6421 tcagaacgtc tagtaatcac taatttaaat acttcaaaaa atatagaaca atataccatc
     6481 ttagtaaaag agaacaatca taaaattcaa atcattgata aaaatttaaa tataaatgaa
     6541 caagttttca tttacaaagc aacaaataat ttactaacac tgcgctctaa taaaaaacaa
     6601 ttagattaca cttttagttt ggattctcca aaaaagctcg tatttatttt gacaacgaat
     6661 gatgggacag aagggacagc taaaccaatt gaatttttta aagctgattg atgaatagtt
     6721 gtttgatatt acaaaacaat aatttcaccg aaggtgaaac aagcgaaact ttacaaaaag
     6781 tggctagcat ttgctagcct tttttgtatt gtggagcgtg ggttttgagg gggattcaag
     6841 ggggagcttg cgcccctttg aaaagcggtt atttgcacca aatacgtagg tatttggcta
     6901 gcaaatatag agcttgccaa gcccttttgg tgagctttac tcattgtgcc tttttaggta
     6961 caatggtatt gcgaacgata cgtccgtagg acgtactgaa agggtgggac ttatgagcga
     7021 acaaaatcaa aatttggcta gcgatcattc taaaaaatat acctatcgat ccaagccaaa
     7081 acaaatcagt tttcgagtga gcgaatctga atttgcaaag ctgaagcagt cagctgaagc
     7141 tttgcaaatg agtgtgccgg ctttcgtgaa agccaaggca caaaacgcgc gtctggtgac
     7201 accgaaagtg gcgccagaca ttgcccaggc aatcgcgcgc gatttagcca aagctggagg
     7261 gaatatcaat caaattgcca agtggtgcaa tacacaccaa catgacgttg ctcctggaga
     7321 cgcccaacgt ttatccgaaa acctaaaaat catgcaaaag gagctacaaa agatatggca
     7381 acagttaaag taagtcgaac cacttcctgt agccgagcaa tcaattacgc agaaccacga
     7441 gcaaccatta aaacaggaat aaactgtgac gtcgattatg ccaaaagtga aatgaaacaa
     7501 attcgcatgc tgtatggaaa agacgaccgt gtacaggcac acttattgat tcaatctttt
     7561 cgaccaggag aaattaccgc tgaaaaagct aatcaactag gggcagagta tgcaaaaaaa
     7621 atagcccccg atcaccaaac agctatttac acacatacag acaaagacca tattcataat
     7681 cacattgtca ttaactccgt taatctggag actggaaaaa aatttcaagc tcatggtcaa
     7741 gcatttctaa acaaatgtta tgacctaaat gacgagattt gtcttgcaca taacttacca
     7801 atcactgagc gaggaaagaa accagaaaaa cgaaccatgt ctgaaataaa attaaaagaa
     7861 aaaaatgaac ctgtttggaa agacgaaatt cggattgcga tcgatcaaac aatgaaaaac
     7921 aaaaaaacca gaacctatga ccaattttgt aattccctca aaacattggg gattcattgc
     7981 ttcaatcgcg ggaaaaattt cacttacgaa ttgatccaca aaaagaaaaa agtacgatcc
     8041 aacagactag gaaaggacta cgaaaaggag acgattttac atgagctgga cagacgagaa
     8101 cgaacctata accaacagcg aaccaacgag ctacgaagag ctcgagctag cattcgcgaa
     8161 actgcagaag aagtacgaag agaacaacga actcttacgc ctgaacaacc ggaaattaga
     8221 ccggctcgaa gaaaccatga acaacacgtt gaaagaggaa cagaccacca acaagaatta
     8281 tcgctctaaa acgttaaaag acatagaaaa cgaaataaag aaacaacgtg cttttctcgt
     8341 tccttatacg caagaaagga tacaaactac gattgattta caagttaaaa aactccatca
     8401 agagctatta gaccagcgaa ataaggaaca gaaaaaggtg cttattcaac gatcattgga
     8461 ttttcttgcc cccttatgtt gcttagtcct gacactgttc gcattttggt tagtatgggg
     8521 aggctaaaat ggcattttat ttaccctatc tactcatttt tgttagtatt tcaggaagta
     8581 tttggctcat ctataaaatt tttcaaacaa gatattcatt aaaaggctct aaaattcgat
     8641 ttaagcgatt tttctttctt ggctgtatat tttccttgat aatcgtttca agcggcttat
     8701 tcggcgtttt agagggaaat aagcgcgttt ctagaagtat tttattagga aatgtcactc
     8761 aaaagtatga atcagcgcgt gataaaaaga aaaaagagca agcattagca caaaaaatgg
     8821 aagagtttac cacttgttat gaggaaatga acgatatttt tgtgaatcaa gaaaaacgtt
     8881 tgacggataa aaacatggaa aaactcactc gtctttatca aaacttgcca gaaaaacagc
     8941 aaaaagaggt tcaagacaac tatgagcgaa ccaaaaaaga cgtgcaatat gtgaaagata
     9001 cgaaagtgga agaagcttgt agtgatttat ttggagatac aattcctttt tcaactagcg
     9061 aacaagaacg aaaagaacga caacaatcag tcacatatga acgctatgaa atcttgcttc
     9121 aacaagcaat taacatacaa aatctgacta aaaaagaaac agctttaaat tatttaaaaa
     9181 gcgtcaagga atggttagat cagcaacagc aaaattgaat ttgattgaaa ttcatgttaa
     9241 actaaacatg caaatgttca aacattagat acacgaaagc cccagagagg tcggattctc
     9301 tggggctttt tttaggttga tgtcgccttt actgatttta cttcttgttt cggttattca
     9361 accaataact gaatacagag atacaaactc ctacaagaag ggggcaaata aacagttcaa
     9421 acatcagata cacctccttc cagtcgcaag actgtcggat aggcgacaag ttattatacc
     9481 ctacaaaatt aaatctgtgt ggcaatttta agtatctctt atttccgggt tgttagggct
     9541 tgccctgacc gcctgtaggg cgctttaaat tccagataaa gttttttata ctaagactgt
     9601 cttaaaacgg cttagacaag ctcttagagc ctcaaccctc ctttttgaac aatggtttta
     9661 gcaaaggagc tggaggcgac tgcgcaggta taatgtgaga gcgtagcgat cacacctttt
     9721 tggcgaccgt agggagcgag gttttgaatt agcagtttag aaaagttcgc gaacctgttt
     9781 aaagaaaaaa taattgtttt tttaggtggg aaattttgtc tccctctttt ataatctttt
     9841 tttaaacctc tttttaaacc tctttaacgt cggctgtgtc cttactcccc caaggtatac
     9901 aaaattttat aggcatgaaa ttatctgttt ataggcatga aattatctgt ttataggcat
     9961 gaaattatct gtttataggc atgaaattat ctgttttagg tattgttttt agacataata
    10021 tgtcctaaaa tgatgtcaat aaaatcattt agactggagt aacaactaa
//
