LOCUS       NZ_OR724980            33303 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Escherichia coli strain Vet2 plasmid pVet2, complete sequence.
ACCESSION   NZ_OR724980
VERSION     NZ_OR724980.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33303)
  AUTHORS   Nordhoff,K., Scharlach,M., Effelsberg,N., Knorr,C., Rocker,D.,
            Claussen,K., Egelkamp,R., Mellmann,A.C., Moss,A., Muller,I.,
            Roth,S.A., Werckenthin,C., Wohlke,A., Ehlers,J. and Kock,R.
  TITLE     Epidemiology and zoonotic transmission of mcr-positive and
            carbapenemase-producing Enterobacterales on German turkey farms
  JOURNAL   Front Microbiol 14, 1183984 (2023)
   PUBMED   37346748
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33303)
  AUTHORS   Effelsberg,N., Mellmann,A.C. and Kock,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2023) Next Generation Sequencing,
            Niedersaechsisches Landesgesundheitsamt, Roesebeckstrasse 4-6,
            Hannover, Lower Saxony 30449, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4; SMRT Link v. 8.0
            Assembly Name :: mcr1-encondig plasmids found in Escherichia coli
            Sequencing Technology             :: PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 10:46:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 40
            CDSs (total)                      :: 40
            Genes (coding)                    :: 38
            CDSs (with protein)               :: 38
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OR724980.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Vet2"
                     /serotype="H10"
                     /isolation_source="faecal boot swab"
                     /host="Meleagris gallopavo Linnaeus f. domestica"
                     /db_xref="taxon:562"
                     /plasmid="pVet2"
                     /geo_loc_name="Germany: Lower Saxony"
                     /collection_date="2018-03-21/2019-02-05"
                     /collected_by="Lower Saxony State Office for Consumer
                     Protection and Food Safety (LAVES)"
                     /note="mcr1-encoding plasmid"
     gene            744..1259
                     /locus_tag="TU527_RS00005"
                     /old_locus_tag="pVet2_000001"
     CDS             744..1259
                     /locus_tag="TU527_RS00005"
                     /old_locus_tag="pVet2_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            1364..1906
                     /locus_tag="TU527_RS00010"
                     /old_locus_tag="pVet2_000002"
     CDS             1364..1906
                     /locus_tag="TU527_RS00010"
                     /old_locus_tag="pVet2_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            1903..2154
                     /locus_tag="TU527_RS00015"
                     /old_locus_tag="pVet2_000003"
     CDS             1903..2154
                     /locus_tag="TU527_RS00015"
                     /old_locus_tag="pVet2_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            2141..2368
                     /locus_tag="TU527_RS00020"
                     /old_locus_tag="pVet2_000004"
     CDS             2141..2368
                     /locus_tag="TU527_RS00020"
                     /old_locus_tag="pVet2_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            2903..3121
                     /locus_tag="TU527_RS00025"
                     /old_locus_tag="pVet2_000006"
     CDS             2903..3121
                     /locus_tag="TU527_RS00025"
                     /old_locus_tag="pVet2_000006"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005058042.1"
                     /translation="MHKDTGQQYVVIFTDNNKIRDYKAGIVPQFGELKQSDVDLVLFYR
                     DEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            3212..3562
                     /locus_tag="TU527_RS00030"
                     /old_locus_tag="pVet2_000007"
     CDS             3212..3562
                     /locus_tag="TU527_RS00030"
                     /old_locus_tag="pVet2_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            3867..4115
                     /locus_tag="TU527_RS00035"
                     /old_locus_tag="pVet2_000008"
     CDS             3867..4115
                     /locus_tag="TU527_RS00035"
                     /old_locus_tag="pVet2_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            4112..4459
                     /locus_tag="TU527_RS00040"
                     /old_locus_tag="pVet2_000009"
     CDS             4112..4459
                     /locus_tag="TU527_RS00040"
                     /old_locus_tag="pVet2_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            4820..5323
                     /gene="taxA"
                     /locus_tag="TU527_RS00045"
                     /old_locus_tag="pVet2_000011"
     CDS             4820..5323
                     /gene="taxA"
                     /locus_tag="TU527_RS00045"
                     /old_locus_tag="pVet2_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            5327..6556
                     /gene="taxC"
                     /locus_tag="TU527_RS00050"
                     /old_locus_tag="pVet2_000012"
     CDS             5327..6556
                     /gene="taxC"
                     /locus_tag="TU527_RS00050"
                     /old_locus_tag="pVet2_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            6633..6926
                     /locus_tag="TU527_RS00055"
                     /old_locus_tag="pVet2_000013"
     CDS             6633..6926
                     /locus_tag="TU527_RS00055"
                     /old_locus_tag="pVet2_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            6923..7138
                     /locus_tag="TU527_RS00060"
                     /old_locus_tag="pVet2_000014"
     CDS             6923..7138
                     /locus_tag="TU527_RS00060"
                     /old_locus_tag="pVet2_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            7494..8113
                     /locus_tag="TU527_RS00065"
                     /old_locus_tag="pVet2_000015"
                     /pseudo=""
     CDS             7494..8113
                     /locus_tag="TU527_RS00065"
                     /old_locus_tag="pVet2_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /name="lytic transglycosylase domain-containing protein"
     gene            8127..8453
                     /locus_tag="TU527_RS00070"
                     /old_locus_tag="pVet2_000016"
     CDS             8127..8453
                     /locus_tag="TU527_RS00070"
                     /old_locus_tag="pVet2_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            8472..11219
                     /locus_tag="TU527_RS00075"
                     /old_locus_tag="pVet2_000017"
     CDS             8472..11219
                     /locus_tag="TU527_RS00075"
                     /old_locus_tag="pVet2_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            11231..11947
                     /locus_tag="TU527_RS00080"
                     /old_locus_tag="pVet2_000018"
     CDS             11231..11947
                     /locus_tag="TU527_RS00080"
                     /old_locus_tag="pVet2_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832283.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            11959..13053
                     /locus_tag="TU527_RS00085"
                     /old_locus_tag="pVet2_000019"
     CDS             11959..13053
                     /locus_tag="TU527_RS00085"
                     /old_locus_tag="pVet2_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            13080..13229
                     /locus_tag="TU527_RS00090"
                     /old_locus_tag="pVet2_000020"
     CDS             13080..13229
                     /locus_tag="TU527_RS00090"
                     /old_locus_tag="pVet2_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227648764.1"
                     /translation="MSLNWILVFVDKHITSGWSRKRKLITQLVILITGITLFKVLGGIS
                     QSWL"
                     /name="hypothetical protein"
     gene            13568..14254
                     /locus_tag="TU527_RS00095"
                     /old_locus_tag="pVet2_000023"
     CDS             13568..14254
                     /locus_tag="TU527_RS00095"
                     /old_locus_tag="pVet2_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            14251..15159
                     /gene="virB9"
                     /locus_tag="TU527_RS00100"
                     /old_locus_tag="pVet2_000024"
     CDS             14251..15159
                     /gene="virB9"
                     /locus_tag="TU527_RS00100"
                     /old_locus_tag="pVet2_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            15152..16270
                     /gene="virB10"
                     /locus_tag="TU527_RS00105"
                     /old_locus_tag="pVet2_000025"
     CDS             15152..16270
                     /gene="virB10"
                     /locus_tag="TU527_RS00105"
                     /old_locus_tag="pVet2_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            16272..17300
                     /gene="virB11"
                     /locus_tag="TU527_RS00110"
                     /old_locus_tag="pVet2_000026"
     CDS             16272..17300
                     /gene="virB11"
                     /locus_tag="TU527_RS00110"
                     /old_locus_tag="pVet2_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            17287..19149
                     /gene="taxB"
                     /locus_tag="TU527_RS00115"
                     /old_locus_tag="pVet2_000027"
     CDS             17287..19149
                     /gene="taxB"
                     /locus_tag="TU527_RS00115"
                     /old_locus_tag="pVet2_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            19158..19580
                     /locus_tag="TU527_RS00120"
                     /old_locus_tag="pVet2_000028"
     CDS             19158..19580
                     /locus_tag="TU527_RS00120"
                     /old_locus_tag="pVet2_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            19564..19869
                     /locus_tag="TU527_RS00125"
                     /old_locus_tag="pVet2_000029"
     CDS             19564..19869
                     /locus_tag="TU527_RS00125"
                     /old_locus_tag="pVet2_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <19982..20176
                     /locus_tag="TU527_RS00130"
                     /pseudo=""
     CDS             <19982..20176
                     /locus_tag="TU527_RS00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            20180..20752
                     /locus_tag="TU527_RS00135"
                     /old_locus_tag="pVet2_000030"
     CDS             20180..20752
                     /locus_tag="TU527_RS00135"
                     /old_locus_tag="pVet2_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            20757..22910
                     /locus_tag="TU527_RS00140"
                     /old_locus_tag="pVet2_000031"
     CDS             20757..22910
                     /locus_tag="TU527_RS00140"
                     /old_locus_tag="pVet2_000031"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            22907..23113
                     /gene="hha"
                     /locus_tag="TU527_RS00145"
                     /old_locus_tag="pVet2_000032"
     CDS             22907..23113
                     /gene="hha"
                     /locus_tag="TU527_RS00145"
                     /old_locus_tag="pVet2_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            23131..23595
                     /locus_tag="TU527_RS00150"
                     /old_locus_tag="pVet2_000033"
     CDS             23131..23595
                     /locus_tag="TU527_RS00150"
                     /old_locus_tag="pVet2_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            23682..24386
                     /locus_tag="TU527_RS00155"
                     /old_locus_tag="pVet2_000034"
     CDS             23682..24386
                     /locus_tag="TU527_RS00155"
                     /old_locus_tag="pVet2_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            25169..25264
                     /gene="dqlB"
                     /locus_tag="TU527_RS00160"
     CDS             25169..25264
                     /gene="dqlB"
                     /locus_tag="TU527_RS00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            25927..26574
                     /locus_tag="TU527_RS00165"
                     /old_locus_tag="pVet2_000038"
     CDS             25927..26574
                     /locus_tag="TU527_RS00165"
                     /old_locus_tag="pVet2_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            26649..26918
                     /locus_tag="TU527_RS00170"
                     /old_locus_tag="pVet2_000039"
     CDS             26649..26918
                     /locus_tag="TU527_RS00170"
                     /old_locus_tag="pVet2_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            27079..27783
                     /locus_tag="TU527_RS00175"
                     /old_locus_tag="pVet2_000041"
     CDS             27079..27783
                     /locus_tag="TU527_RS00175"
                     /old_locus_tag="pVet2_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_225236207.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLQIINTLKCIFSMGLRND
                     CLIKFLKYFCIA"
                     /name="DUF2806 domain-containing protein"
     gene            27877..29502
                     /locus_tag="TU527_RS00180"
                     /old_locus_tag="pVet2_000042"
     CDS             27877..29502
                     /locus_tag="TU527_RS00180"
                     /old_locus_tag="pVet2_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCR-1 family phosphoethanolamine--lipid A
                     transferase"
                     /protein_id="WP_318838740.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MIQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="MCR-1 family phosphoethanolamine--lipid A
                     transferase"
     gene            29550..30296
                     /locus_tag="TU527_RS00185"
                     /old_locus_tag="pVet2_000043"
     CDS             29550..30296
                     /locus_tag="TU527_RS00185"
                     /old_locus_tag="pVet2_000043"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_077248209.1"
                     /translation="MGGGVGFVMPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVA
                     DWFYLGMGHWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKW
                     LVPVTKFRVRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRN
                     TIPDKCFPAAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSH
                     TVVSMMLAWAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(30687..31214)
                     /locus_tag="TU527_RS00190"
                     /old_locus_tag="pVet2_000045"
     CDS             complement(30687..31214)
                     /locus_tag="TU527_RS00190"
                     /old_locus_tag="pVet2_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(31349..31753)
                     /locus_tag="TU527_RS00195"
                     /old_locus_tag="pVet2_000046"
     CDS             complement(31349..31753)
                     /locus_tag="TU527_RS00195"
                     /old_locus_tag="pVet2_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            complement(31766..32632)
                     /locus_tag="TU527_RS00200"
                     /old_locus_tag="pVet2_000047"
     CDS             complement(31766..32632)
                     /locus_tag="TU527_RS00200"
                     /old_locus_tag="pVet2_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     ARG             27878..29499
                     /name="mcr-1"
                     /gene="mcr-1"
                     /gene_name="MCR-1 family phosphoethanolamine--lipid A
                     transferase"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="99.82"
                     /coverage="100.0"
     ARG             27878..29502
                     /name="MCR-1.1"
                     /gene="MCR-1.1"
                     /gene_name="MCR phosphoethanolamine transferase"
                     /tool="rgi"
                     /drug_class="peptide antibiotic"
                     /identity="99.82"
                     /coverage="100.0"
     Typing          complement(31767..32638)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5328..6553
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11960..13026
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
     Typing          13569..14251
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          14252..15156
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          17288..19146
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttaacttta acttattgaa ataaatataa tattcgtgcg tactgatcct aatgtcacat
       61 cgcaaaaatt ccgcttcctc gctcactcag tcgctacgct cctgccgttc ggctgcggcg
      121 agcggtatct cctttctcaa aaccagagag attcagatca tatgatccgt tatttgatcc
      181 tttttggatc ttccactaag aagccctgaa tcgcgctctg ccgcttcgtt cgatacaacc
      241 atgaggaaaa ccatgcgaaa tgaaaaaaat cgcttaaaat gcgattcaga gcgttttagg
      301 gaggttctgt tagttctcct gcattgcgcc cgtagttcgc taacgctcac acgcgcagta
      361 cagcgccccg ccatgctgca atgcaccagg gcggggtaaa ctcgcttgcg ctcgcgctaa
      421 ctgcggcatt ctgcggcgca aatcagcata atcatggcct gctggtcagt tgggctttgc
      481 agggtgaagg gatggcctgt gcaccctggt acgttcgtca gcggctgcgc cgcgcttcgg
      541 ggcgatgtgg ctcctgtgct gccctggtat tttcgttcgc cccttccatc cgcttacgcg
      601 gattaaaaaa cgtgataaca atcacttatc gagtaaaaaa acgggagaaa acttgcaaat
      661 acgacttaaa ggtcgtatta ttgtttcatc gaaaggggaa tggcccctct cacaatagcc
      721 cagcagggca ggagattaaa aaaatgaata tccaggaagc attaaacgtt tttggattat
      781 ccggcgaatt aactgaaaaa gatatcaaag cagcatacag aaaagccgct ttaaaatatc
      841 atccagatcg taacccgtta ggggctgaac tgatgaaagc ggtaaatgca gcttttgatg
      901 tattgatggc aaatattgat aaaataaatc agttccagag cactgatgaa catgcacgat
      961 ataattacgg tgatgacctg gaaaaagtat taaacgttct ttctggttta tctggtctgg
     1021 tatttgaagt gataggtaac tgggtctgga ttagtggaga aaccattaca cataaggaaa
     1081 ctttaaaaga aatcgggtgt aaatgggcgg caaagaaaaa acaatggttt tatcgtccag
     1141 acgaacataa aagttactgg aatcgtgaag aacacacgat agaagaaatc cgcgcaaaat
     1201 acggtacaac cggacagcgc agggcgacag ggtggcaacg cgtggaaacc agagcgtaac
     1261 cagaacgggg gcgagaagcc cccgatatta tagccagccc gtcttttggg gcatcgaacc
     1321 caaaaagacg ggaacagcag ccacgtagca aaaagggaaa cagatgaact ctttttttga
     1381 acaatatcac cctgtctttg aagttgtctg ccgtatcctg gggaacggct ggcgcgtgaa
     1441 caaacttgat gattgttcgt ctcgtataaa actgacgtca ccgcagttta aaaattactc
     1501 tgtgcatatt cggatggaaa aagaccgctt ttctgtggtg gggagtgtgg atagtcgttc
     1561 ctggcgtagt ccgcatcatg tttgcacgtt atccagaaaa cggaatcccg ttgatattgc
     1621 agccgatatc gagcgaaaaa ttctggtaaa tgcttcgcag gaggtgttac aggcgattga
     1681 gtatgagaaa caccaggtgg aaaaaaagga cgaaattctg atcctgaaag gtatgttatc
     1741 gcaacttgtc cagcttgaaa gctggtatgg ggcattgaca ggctttaaag ctgaaaacgg
     1801 attgaacggt aaagtcaccg aacagggcga cagttatgat ttacagattc ggggcttgag
     1861 tatagatcaa cttgttaaaa ttacagggta tttgaaacag ttatgagaaa aaataaaggt
     1921 gatgtgactt attttcttga aaaagaaggt gacaattaca ggcttacaaa aaggataaaa
     1981 gccagaacaa atgttaaaat cgggaacaaa actacgaaaa ttacattgta tgatgctgta
     2041 ctgaatgaga atgaattaca acatattgat tttacgtgtg ccggattaag ggaagacgat
     2101 gagacgccag ttaagaattt aattaaggag tttatgttaa atgaaacccg ataatacacc
     2161 tgaaaatgta aaaaaattac gtttaaaagc aggattaaca caaaaggaat gcagtcatat
     2221 ttatggtgtt gggctaagaa cctggcagaa aaaagaggaa gttaatactc aaaacagtca
     2281 aagtctgtca ctggttgagt ttgagtttct tttgttactt gcaggagaac atcctgaata
     2341 cgtgctctgc aaaagggagt caaaataaaa tgacctgtga tggttttata ttagtacctg
     2401 gagttgcgct cgtagctcgc tgacgctcac acgcgcagta cagcgccccg ccatgctgca
     2461 atgcaccagg gcggggtaaa ctcgcttgcg ctcgcgctaa ctgcggcatt ctgcggcgca
     2521 aatcagcata atcatggcct gctggtcagt tgggctttgc agggtgaagg gatggcctgt
     2581 gcaccctggt acgttcgtca gcggctgcgc cgcgcttcgg ggcgatgtgg ctcctgtgct
     2641 gccctggtat tttcgttcgc cccttccatc cgcttacgcg gattaaaaaa cgtgataaca
     2701 atcacttatc gagtaaaaaa acgggagaaa acttgcaaat acgacttaaa ggtcgtatta
     2761 ttgtttcatc gaaaggggaa tggcccctct cacaatagcc cagcagggca ggagatttaa
     2821 aatgaaatgc tcatctgttt ttacttcaac cactaatcat gttttcacat tcgaacgagt
     2881 aacgctttgc actattatcc ttatgcataa ggatactgga caacagtatg ttgtcatatt
     2941 tactgataac aataaaattc gtgactataa agccggaatt gttcctcagt ttggcgaact
     3001 taagcagagt gatgttgatt tagtcctttt ctacagggac gaatatgaaa aatattttga
     3061 ttcattaaaa gatggcgatg aatgcttgag ttttaaagat tttattgagt gtctttgtta
     3121 agcattatga atgcaggggc atccctgcat tttctggaaa tttcaatatt gattctgaaa
     3181 ttgtctatta acatcaatgc aagggaaatt catgaaaacg ttaaaacaag ccgcaatgca
     3241 gtttgcatct gatctgcgta ataatcgctg ttttaaagct gcttataatg atgctctgtt
     3301 agagcttgat cgggaagatt taaacgccat tgtacaatgc acatttcttt ttgagccaaa
     3361 acgaacatta gccgaacttg ataaattaat taaaaagata gaagtagaag aagattatga
     3421 gttttgtgta tcccttgata aaggtaaagg attacacgaa gtcagaacat ttaaaagtga
     3481 gcgtgaggcg ttggatgctt atcgcatttt gactctttac ggatacacag taactataga
     3541 aaagaaggag ggtaatgact gactattcaa ggtgtgatca ggttgttcca ccaacctgat
     3601 cacgttatcg acaatcaaaa ctgaatgagg tttttaaatg tcaacgcggc agatcgtaca
     3661 gcttgctgat tcccttgtca aaggctggga aaccagaatt ccggcaatga aatttagcga
     3721 atgggatcaa ttccggtggt ggttaaacta cttgcaaggg tatgaaatgt tttaaataaa
     3781 aaaaggggct ttctgcccct ttgtattttt tgacaaaaat gccatgtgta actataatta
     3841 gttgcatatc tataggggat ggatttatgg ggaaaacaga taagctactg gcaaagtttt
     3901 taaacagtaa aaaaacgttt gaatgggatg aactggtcgt tttgttttcc tctttgggat
     3961 atgtcaaaaa ggaaatgcag gggtcaagag tgcggttttt caatgctgaa atcaatcaca
     4021 ccatattaat gcatcgtcca catccggaaa gttatatcaa aggtggtacg ctgaaagcaa
     4081 ttaaacagaa tctgaaagag gctggattat tatgaaacat ttaaaatata aaggatattt
     4141 aggcacggtt gaacccgatt ttgaaaataa tgtcctgtat ggaaaactgg cgtttattcg
     4201 ggacctggta acttatgagg cttctacatt agctgaactg gaacaggagt ttaaaacatc
     4261 tgttgatctg tatttacagt cttgtgtgga ggatggaaag gaacctgata ccccgtttaa
     4321 aggcgtgttt aacgtcaggc ttgatccaga actgcatcgt cgggtagcag aaatggcgat
     4381 ggaagaagat ttatcgttga atgcctttgt taataaggct ctggaaaaag aagtcagcaa
     4441 tcatcgcgca ggggcttaat tgcccttgtt tttatgccct gtaagggcat gggaggcgct
     4501 ttgcgccgga agccccgcag cgcagcgagg acttaaaaag cgttacggct tcagccgtaa
     4561 caattcccag ccctgccagg cctgtttttt ctttcacaaa tctttcagct atacgtttgc
     4621 ctgatggtcg aagacgaaag aatctttcag caacgaaatc tggacatatg cagcgacgat
     4681 tttatagcca ggcacagtgc cacatatggt acaatcaatg caggatagac aatccgatgt
     4741 caaatgttaa catttgaatc agattgtcca aaaacgatgt ttttgcctgt gcctgccgca
     4801 ggctctgttg gggtgactta tgaagatgat agcgttcagg ctaagtgatg atgagttaaa
     4861 atttgcagaa cataacgcta ttttaagtgg atttaccagt atcaatgctt ttgctaagca
     4921 taacgtgctt aatattgaaa ccaagcctgt taacatccca gttaataatg aaccagcaaa
     4981 gctggtatca gtgcgtcttt accctcatga aatagaactt gttaaacgta atgctgctct
     5041 tcacggcatg agcatgagca gagaaattgc tattcgtgtg cgtcagtctt tgttaaagag
     5101 tgaagtctgt ctttaccctg atgaagttaa ggaacttaaa aaactatcaa ctgctgtaga
     5161 tcgtgttggt cgcaacattc attttatcat aaaaggtgaa agattctgta cggttaacga
     5221 tccagatttc agaaaagacg ttattgaagt gattgaactg tgtaagcaaa ttgattcaaa
     5281 acttgaaacg ttaaccaaga gtgttgttaa cagatttggg tgatttatgg gtgtgtatgt
     5341 agaacaggaa taccgtatta aacgcgctaa agggactggg cgtgatccga aatctcctaa
     5401 gttatcggga agacatatac atgccagtaa atcggctttt aatcataaag tcagacatgg
     5461 tgcggacaag aaagcatata cctggacacc tacaaaagaa gtcactttta aaatcactgg
     5521 ctcaggtaaa acagccgcag gcatcaaaaa tggtattgat tacattaccc gtaatggtga
     5581 acttgaggct tactgttatg atggaaaagg ttcagagcaa acagggaaag gagaagactt
     5641 taatagggag tttacttcaa ctttatctaa aggaaatgat tactccagga catatcgtgg
     5701 agaaaacatc gatcatgtga aaaacatggt cttttcacca ccaccagaag ctggagtaag
     5761 cagggaagac gctctgaaag ctacggtaga atttcttaaa gagacttatc caaaccatgc
     5821 tttcgttgcc gtataccatg atgataaaga agatcatcct catgtacatg ttaatattaa
     5881 acttcgtgat gaagaaacag gaagaagatt aagacttact aaatctgaaa ccaggaagtt
     5941 cagaaacggt tttcatagaa aactgaaagg tatgggatat gatgttaccg ctacctggaa
     6001 aaaagatccc gaacgtaaac gggagattga gcgattacag gctgaaaatc cgaaaagact
     6061 ccggaatgtt tacaaggtgg tggattttgg ggagacttca tatcagaata aggcagggga
     6121 aaaacgaaca cctttcctca cttatgaaac tttaaaaggt ggaaaacagg ttacgatttg
     6181 gggtaaagac ctgaaaaatc atttcgagtc tgaaaagctc cagccaggaa cgcttatcaa
     6241 agtaaaaaaa ctggctccta cgcttgtacg tagtccaatg tttaatgatg atggaactgt
     6301 tgcaggctac agggaaacgc atcgcaataa ctggcagatt gaaaatattg cccttgagcg
     6361 gaacagagaa cgtcaggttc atgagcgtga aaccaggcag ccggaagaga gcgacgttaa
     6421 aaagcagcta tccagaaaac atgagcaagg tcacaatatc gggtttgctc ttgaacacgg
     6481 gtttacaaag gattcagaag aacataaaaa actaagaata cagcaagaga ggaactggaa
     6541 aggcctggga ttttaatttg attttggcgc aggtgaacaa agcggctact ttgctcacct
     6601 gctgatcaca acaacctacg agggaggtta tcatgactga gcatgatgct atctgcattt
     6661 ctggacttca tcaaattttt agtgatgaag aacatttgag tgagcaacaa aaagacatta
     6721 ttctgatgta cgcttatggc tataccctga atgaaattgc tgatttcaaa ggactaaagc
     6781 cttcaactgt cagaaaatat ctcgattctg taagagctga actgggaggt gtctcgttgg
     6841 cagggatcag aacactggta ctcattcgga ctaatgccct tctcgtgtca agtctttcca
     6901 ggatcagtga gagggggaat ttatgaaaaa atatctgctt tttgctttgc cgttcttcgt
     6961 tgttggttgt agtgaagaag taaaaagtgt ggattggtgg gggcagcatc ttacggaagc
     7021 taaacaaaag caggcagagt gtgagaagtc tggctctgat tctcagaact gtaagaatgt
     7081 taaacaagca ttgtttattc agtctcagaa ggatgctcca gtccctacat tcgattaact
     7141 aatgtttcaa tcatactaga gcgcccatat gggcgctttt ttttgcatga tcacacgaat
     7201 cataatatga ccattttgtc aatgttattt gatcgtattg ttgagcattg ttattttgcg
     7261 aatcatgttc gcatctaaaa caaatccaaa acacacttat agtggattga ttaaatgaag
     7321 gagaaatcag ataagtcatc ggaaaaaggg gtttatgtca cgataaaact cgatcccagg
     7381 ttgaatgcat ttatcgaaga gaaaacgaaa acatctttgc ttagtaagag gaagcaaatt
     7441 atttacttat taatgcagag tatgcgtatt gatggatata tggagcgtta gctatggcgt
     7501 ctttattccc agccttgttg ctgtgtgcgg cgtcagttca tccggatacg ataaatgata
     7561 ttgcacgggt cgagagtggc ttcaatcctt atgctgtggc tgaaattatt cctgaaaggg
     7621 aacgcggtag ctatggcaaa agtgttatca gtcatatgcc caaaagtaag gaggaagctc
     7681 taacagttat cagagaaatt gaaaacagaa aaagacgtta ttctgtcgga ttgatgcaga
     7741 ttaccagcag taatttttct ttttattcca cgtccgcaga aaaactcctt gattcgtgtg
     7801 aaaacttgtc tgttttcgaa aagattatcg tggattgtta taaacgaggg aggagcctgg
     7861 agaatgcgct tagttgttac tatacaggaa attttagtaa cggtaagcga aaagaaaaag
     7921 aatttaataa tacaagttat gttgagcgaa ttggttatac agggaacgaa aaaaatatgt
     7981 tgtaccaggt acaagaagca acgggggaga acagcgaaaa aatcgttcac acaatgcgtc
     8041 agttatctgg cccgaaacca ttctgaaatc tgcttttgtt gataactcac atccaacaaa
     8101 agttattaat taacaggact tataaaatgt catttaagaa atcagtatgt aatcgtatca
     8161 gttcatctgt cactgctgta atgcagcgta aaaataaggt agttatggca gtcgccgggg
     8221 caacagttgc atcaccggcc tttgcggatg gattttcaaa agcggaaaca ctgctggaaa
     8281 aggttaaaac agggcttagt ggcctttcac tggtcacggt gactattgcc tgtctgtggg
     8341 tcggctacaa ggttctgttt ggtggaagca cgattcgtga atgctcacct atcatcatcg
     8401 gggcaattgt tatcgccagt gcggctgaag ttgcatcaat gatggttaac taataaagcg
     8461 gggacgatag tatgtctaca ctttataaag cgatgacacg ccctgctatg tatgtgggtg
     8521 ttcctgtcgt ccctcttact gtggttgcag gggcgttatt tctggcgggg gtttatatca
     8581 gcaagctgat ctggctggct attcctgtcg cggtttttgt tctcagaatg atcactaaac
     8641 aggacgacca catttttaac ctttactttc ttaagctgaa aatgctcgga aactcagtgt
     8701 gtaatcgttt ttttggcgca cgagcgttcc tctccggaca atatgaagct gttgaaattg
     8761 atgagtttgt taatgccatg aaactgaatg agcgaatcac tactggtaaa tatataccct
     8821 attcaagcca tgttgataaa aatattgtca aaacaaagaa cggtgattat gtagcgacat
     8881 ggcaactgat ggggattaac tttgagtcaa tctctgcgga gatgttagaa actattgact
     8941 cacaggttgc cactcttgtt cgttcatttt ccggactgcc cgtttcattt tataaccatt
     9001 catgcagggc gagtttttat gatgccttta ccacaaaatc aggtaataaa tatgctgata
     9061 taatatcaga ttgttactat ggttcgatga agaaaaataa attcaagggg aacactcttt
     9121 actttacgct gatctacaga ccagatgggc gtgtagaaaa actggaaaaa cgcaaaaaaa
     9181 gtattaaaga aaagaaagat gatattaaca ttcatgtgaa gagaatgaat gaaatgatta
     9241 atacattctc tggcgctctt gataaattta cctgtaaact tcttggtatg tatgaggaga
     9301 acggaaaggt attttcttct caactgtcat tctataatta tcttctgaca gggaagttgc
     9361 aaaaaatcag agtcactgat tctccggtgt ataacgttct tggtggagtg gatgtatttt
     9421 ttaatcatga taccggacag atatgccgca ttgatggaaa taaattcttt cgttctattg
     9481 aaattaaaga tttctgtagt gagactgcat ccggtgtttt tgatgtgctc cagtattctg
     9541 atgctgacta tattattacg cattcatata catccatgag caaatcagaa gcattgagta
     9601 ccattaaacg tgcggaaaaa cagttaaaga gtacagaaga tgatgcggtt actcaattgc
     9661 aggaactgga aaaggcaaaa aatgatattg tgtccggtga tatatccttt ggttactacc
     9721 atttcacgct aatggtaatg gcggacagta tcagggagct tgacgaatct gtaagtaaaa
     9781 ttacggcaga ctttactgac ctgggcatta ttccggcatt gtcaaccatg tcattgccag
     9841 cggcatattt tgcccaactt ccggcggttt ttcatttaag gccgcgttta tctccggtat
     9901 cgaatgtaaa ttttgttgaa ctggcatcat tccataattt ctaccagggg aaacgcgata
     9961 aaaactgctg gacagaagcg gttgctattc tcaaaacacc aagcaaacag gcgtattatc
    10021 tgaatttaca taactcagta ctgtttaaag atgaaacagg tgaaaagaat cttgctaata
    10081 ctaaagttat aggaacggcg ggttcaggta agacaatgtt tctgtcatat cttgcctgtt
    10141 cattacagaa atacaataac cccgaaactt ttgctgattc tgcaaaaaat aagaagttaa
    10201 cgtgtgtctt tcttgataag gacagagggg ctgaattatg tatccgaatg cttggtgggg
    10261 aatattacac cgtaaaaagc ggtgagccga caggatggaa tccttttgct ctggaggcaa
    10321 caaaacgtaa ccgtattttt gtcaagcaac tgatggagat tctgtgtacc agaaatggtg
    10381 aaagattatc gacaagagaa agattgctta ttagcgaaag tgttgatgct gtgatggatt
    10441 ttcctcctgg cgaaatgcgg gaatatggca ttaccagaat gcttgagcat cttatgcaga
    10501 gagatgatcg ggatgagcag gagaacggga ttatccttcg tttgtcccaa tgggctaatg
    10561 gtcaggctca tggctgggtt tttgataatg ctaaagacac attcaatatt cagcatgtca
    10621 ataattttgg cattgatggc accgagtttc ttgatgatcc gatggtatgt gcaccaatta
    10681 ctttctatct gctgtatcgc attacgcagc ttctggacgg tcggcgtctt gttatcttcc
    10741 tggatgagtt ctggaaatgg ctacaggatg aagcattcag cgactttgtc tataacaagc
    10801 tgaaaacaat tcgtaagctg aacgggctgg ttattccggc aactcagtct ccggatgaaa
    10861 tactgaagaa taagatttca cgggctgtgg ttgaagtatg cagcaccagt atttatctgg
    10921 ctaaccctga tgccgattac aatgattatg tggaagggct taagctgaca ccagaagaat
    10981 ttaatattgt taaaaatctt gatccaatgt cgagacagtt cctgattaaa aaaagcagtc
    11041 ttaaaaaggg ggatggtaaa tcattttcag cacttgcgac actcgattta tcgggacttg
    11101 gtggatatct gaaaatattg tctgccagtg cagataatct ggaaattttt gaaagtattt
    11161 atcatgaagg aatggagcct gatgactggg ttcctgaata tctcgaacgg gcaatctgac
    11221 aggtgaattt atgaaacgtg taaaaacatt gatgctgata tcattattga cggcttcatt
    11281 ttatagtagc gcaggaattc cggttgctat tgatgccagt ccggaatggg ctgttgaagc
    11341 cgcaagatgg acagaacgac tcaaacaatg gtccgaaact gcgcaacatt atcagagtca
    11401 gattcaggca tataaagacc agttagccac ggcaacaggt ataaggaata ttgcggcatt
    11461 cacaaatgaa ctaagtaatc tacaatcaga actgacaaat atttataagc aggggaacag
    11521 ttatatttct gattttacca gtaatcccga aggtgcattg agcagccagg ctaaaaatct
    11581 gttcagcaag tatggagcct tcgatatgtg taatacgggc tatgaacgta atgataatct
    11641 ctgtaaagca cggattgtgt caacggctgc cagtattgaa cagggaaatg agatcaataa
    11701 gcagttatca agtgcaatgt ctcagattca gagtttaagt agccgaattg aggcttcaaa
    11761 ggatatcaaa gaatcccagg atttggctaa cgcattacag gcgcagtcgt taaaaatgca
    11821 ggcgataaaa atgcagtatg atgtctggaa taataaaaat aaagcagatc atgagatgtt
    11881 ggtcacacaa gaacaagagg cctttattaa acagcagaaa gaagctgaac cattaacttt
    11941 tgattaaaag gtggatatat ggctcagggg tttttcgtta aatataattc taccgttatg
    12001 gattcggtag ataaaatcag ctccagttat caaacacaat ttgctaatga tattatgtcg
    12061 ttagcaacgg tatcggttac gttatatgta ctatggaaag gatatcaaat acttgccagt
    12121 aaaactcaga cgcccttgca ggatttagta tgggatctat caaaatttgc aatcataatc
    12181 atgtttataa ctaatgcgga tgggtattta actgcggcta ctgatgcgtt gcaaggcatg
    12241 aaagatggtt tttctggagg ggtgagtgtc tggcaaactt tagataactt atggaaaagt
    12301 acgcaaaatc ttggtgcgga aatatactct ttagataaat caacatatgt taaagatcaa
    12361 ggtgtagttg gtcagttttt aatatggact ggctcgctta ttttaatggc tgttagtgtg
    12421 gttgtttttt taacagcaga tgtaacgatg aaactcctga ttattacagc accgatattt
    12481 atcttttgtc tcatgtttgg ttttattcgg agcatgttta ataattggtt acaatcgtta
    12541 ttctccagta ttctgacagt tctatttgcc agtttagtga ttcggatagc aatggatttt
    12601 caagggatga tcctttccca tgctattcgt gctgcacaaa cagggaatgt aaatcttgta
    12661 tctacaggtg ctatgggctt tatggctggg tttttggggg ctttattggt tcttatagct
    12721 aaaggttttg ctgtccagtt agcaggtgca ggtgttgaag gtgctgttca gggcgcagcc
    12781 atgatggggc tgggtgctgc tggtatggct actggtaagt ctctgatgct tggcggtcgt
    12841 gctggtttag gttttggctt agggatggcc ggaagaacgg gcatgaattc tttatcaggg
    12901 aaagcaggga atctgatggg gcgtggtgcg cgaacagccg ctgaatgggc gggtgaaaag
    12961 ggatatcagg cgctggctcc gacaggtgct ttggggatga aggcgagaag acttgcatcc
    13021 ctggaaaaag cacgagcgag gaacgccgca tgaaaataat gaaacgtttt ttttatgtga
    13081 tgtccctgaa ctggatactg gtgttcgttg ataaacatat cacctccgga tggagcagga
    13141 agagaaagct aataacccag cttgttattt taatcacggg aattacctta tttaaggtct
    13201 taggtggaat cagtcagagc tggttgtgac atgcttcatt attttgtcat gatactttat
    13261 tttgtcctaa tccttctttt aggagtgttt acctgcttcg caggaataag acttggttta
    13321 gggcttgcgg ggaaatggag aaagggtatg gatcttcgct ttttgcttct tattggtggg
    13381 attctttttc ttcatcttgg cctttatctc ttaaaggtgt tcgtgaacgc ttaataaggt
    13441 gaatttatgc gatgtgtgat tcttttgttt ttcctgtttc agatttccgg atgtaccaca
    13501 aggaataacc ttccttctga tgtttccggc gaactggaac ccattaacca cagccaggta
    13561 atcagttatg agtgaaaaaa tcgaaaagaa gattgagaca gctaaatcct tcgaaagaca
    13621 actctatgca gaaaatgaac ggtctaagaa aaatgcatgg cgtgttgcag tggcagcatc
    13681 attactgtcc ttatgtcttg gggctgcaat ttgtgttctt gtgccattaa aagaaaaaga
    13741 gcttgctatt gtggctgttg gcgaaaaaac aggccgtaca gaactgatta ctcaggttaa
    13801 acaggaaaag atcctgcaat ctgaagcact tgggcggtat tttgtaaaca cgtatattac
    13861 tttacgtgaa ggttataatt atccgtctct tcagtatgat tatgagactg tacagctata
    13921 tagctcgaat acagtaaagg acgattatct tcgtctgtat aacagcgata tggctcctga
    13981 taaaatttat cataataacg gttcttacgt tgcggttgag gtcatctcca atattatttc
    14041 tgacgcaacc gcgccagata aactggcctc tgtgcgcttt aaaaaaacga cgagaaactt
    14101 cacgaccgga caggtttcag tttcatactg gacggctcgt gtgacatatc gttttgaacc
    14161 agaaaagtcg gttaaaagtt ccagtcgtga gcttaatcct ctcggtttta ctgtaaccag
    14221 ttaccagaca gatcgggaag tcagggggga atgatgaaga aattatttat tgctacatgt
    14281 ttattgcttc ctgggcttgt gtattcagca gcgactccgt taccttctgg atttgatgca
    14341 aggatgcaga ctgtgagtta taacggtgcg aatacgaccg ttatccggtc aaagacagga
    14401 tttctcacat cagttgtttt tgatgaaggt gaggctgtta tcagtgcaaa ggctggtttt
    14461 cctgccggat gggaaatcac aaccgatgat aatgttgttt acatcaatcc gcgtcccgtt
    14521 gttcaggaac aggaaggcga tgaaggcgaa aaactgaaaa aggttttcca gccaacagaa
    14581 aaggaatggg atacgaatct ttttgtcaga acaacaaagc gtatttacag tctggatcta
    14641 atccttctgt cagaagaaaa acaagcgcaa cctgcttatg ttgtacaatt ccgctatcca
    14701 tcagaaatag cgaaaaagaa tgcagaagaa gtaaggctgg caaaagaaaa acaggaaaaa
    14761 ctcagacaga agaaactgat ttctgaaagt tttgaaaaag ccgatgcgcc taaaaactgg
    14821 aattatttca tgcgagtcaa tgaaaaatat gacagtcgtc gtatcgcccc tgattttgct
    14881 tatgataatg gcatatttac ctttttaggt tttaactcag ggaaagtatt cccagctcca
    14941 tttgctgtaa gagatggtca ggaacagaca cttgctttta atgtggaaac aaaaggcaag
    15001 tataaaatta tggttatcca caacgttaat gacaaatttg ttttacgcta tggaaacagc
    15061 gttgttggtg ttgtgaataa atctttcgga aaagtattaa ccgaccagag gaatacatct
    15121 tctcctgcgg tagaacgtgt tgaggtgaat aatgactgat atgaaaaaaa cagatgattt
    15181 agttgatgat gcgaatcagt caaagctgga aagtaaaccc gttcatgata tcggtgatat
    15241 caggaacaaa aataatcgta tgcgttctgg ctttctgttt ggtctgatgg ctgtattagt
    15301 tattggtatt tttgcgctga aaacatataa aaattatttt gcagatgacc agcagaaagc
    15361 atcagaatca acaggcgata ccagcatttc tcaggtaagt aaaatccgta ctggtcttgg
    15421 tcagaatttt gaccctgttg agaacaaagc aattatcaat ccaggcgcta ctggttctgg
    15481 taatactgtt tctgacggtc ataaagaagt gccacaggag gggttcagga aatatttgtc
    15541 aatacctgtt gctggtcagg gagggagtca gactggcgcg agtggcagca gcaggacatc
    15601 gcagacatca gagccacagg aagaaaggaa agaatcaaaa gaaaatgtgc ctggtaaatc
    15661 aggaatgaaa gtaactgcga ttaatctcga tcctgatctc tatattgagg aaaacaggct
    15721 tataccgtgt gctttaacca cacgctttgt ttctgatgtt gcaggtcgta tcagttgtgt
    15781 ttttacagaa gatgtatgga gtgctaatca ccacacaaaa ttactggaac agggaactaa
    15841 ggcttttggt cgttaccaga ccggaacgct gaatcatgga cagggcagaa tgttcgttat
    15901 gtgggagcag ttgcggacgc ctgataataa acgaatcgat atggtaaata cagcagcagc
    15961 tggtcccctg ggagaagcgg gcattgatgg atggatcgat tctcacttct gggagcgttt
    16021 tggtggttct cttatgctca gtatggttca ggatgtagcg gcggcggcgg cggataatgc
    16081 acctggtaaa gatcgtaatg tggattatac cgaaaactca cgacaggcaa tggctgaaat
    16141 ggctaaagtc gcacttgaga attcgataaa tattcctcca acaatgtata aaaaccaggg
    16201 tgacataatc actattatgg tcggggaaga cattgatttt tctgatatct atgagttaaa
    16261 ggtaaaatag tatgagtggc gttattcctg tcagcattag caataaatcg ctcgattttt
    16321 ataaaaatca agtattccag tcttttcttg atattgaagg gctaacagaa attgctgtca
    16381 accgtcctgg tgaaatctgg acggaaatta atggtgactg gacattccat aataatgaca
    16441 gtgtgacctt tgatttttgt aaacgttttt ctcatacgct ggcatcattt cggggagatg
    16501 aaatcggcga gaccaagcca ttactttcag caacactgga atcaggagaa cgtgtacagg
    16561 tcgtttttcc tcctgcatgt gagagaaata ctatttcaat cactatacgt aaaccatcga
    16621 cacggcagat aacacataaa gaatatgtgg cgaacggttt ttatgattat gtccagtcag
    16681 gaaaaaaaca caggacatac gatgatgagc ttcttgattt attcagagcg agaaatatcg
    16741 ctgcttttat ggagcttgcc gtagcagcag ggaaaaccat tgtatttgcc ggagcaacgg
    16801 gttccggtaa aacaacttat atgaagagtc ttatcgattt cataccattg aatacacggc
    16861 tgattacgat tgaagatgca gaggaaatca aattttttat ccataaaaat tatgtgcatc
    16921 ttttttaccc ttcggaatcc ggcagtgatt ccggctctat cataacagca gcgaaattaa
    16981 tcaaatcctg tttgaggatg aaacctgaca gaattttact ggcggaaatt aaaggtggcg
    17041 atgcctggga ttttgtgaaa gttgctggtt ctggtcacag tggaagcatg acgtccattc
    17101 acgcggcctc ggcaaaagat gccatcattc agatggtgac aaaatgttat cagaacagag
    17161 aatgtcagaa tcttcctttt gatgttcttc gtaaaatcat tatggacagt attgatattg
    17221 ttgttcatgt aggcagggat ggtgttgtca ggcatatgag cgacatctat tacaaagggg
    17281 ctgaatgtga aaacttttaa taagtcgcaa tctgttttta tattcgctgt tatgcttgtt
    17341 gcggcatggt ttgcaggttc atttattttc tttgtgcttt acttcagcca gataaagaat
    17401 ctgaaaccat tgaaggctgt gttcagatcc attgatgcgt acaggatgga tatttttaca
    17461 gacagcctta ctttatctgt tgtgactacg gatatcagag ttttagcctt tgttgcactt
    17521 ggagcaggat ttgtcgtgtc tcttatcatc cctgttgcgg cgttgataaa actgaacgag
    17581 aagaaagaaa atattttcgg tgatgcgcga tttgcaacaa ttaatgatat tcgtgagtct
    17641 aacagtttca cgcttgacgg tgacgaaaaa gacgggatta tcgttggtat aaaagacaag
    17701 aaaatcatac gctatgtggg agcagcattc agtgctatgg gagccggaac aagggctggt
    17761 aaaggtgctg gtatagtcat cactaatctt atgaagtact ggtggagtgt tattattctc
    17821 gatccaaaaa gggagtgttt taatatcacc agtcttatca ggaaggtgat tttaggtcat
    17881 gaggtttata aatttgatcc ctttagttcg gtaacgcacc gctttaaccc tttgtattat
    17941 gtcagcatgg gaacgagtga aggttttaat cagcttgaaa atctttcgtt gattatttac
    18001 ccctataaga cagatggtgc ggatgctggt agctatctta ataagacggc gggtggtgtt
    18061 tttaaatctt atgctgtcgc tttatggttt atgattaaga atgataaggc tggactaaaa
    18121 acgcttgata ttgagcctgt cttctcaatg tctaaaatta atcagctttt tgagcgtgca
    18181 gaacccgaac atcttctttc attcatgaat gatattcgtt cagaattaag ggggaaagac
    18241 aaaacgcttg ctgatattgg tgtcgctggc cttaaggcat tcattgagat ggaggataaa
    18301 acaaaagcac aactcaaaag caactttctt aatggattat caccgtttgc taaccccaat
    18361 gtggcaaatg ctactgatgg aaatgatttt gatttgcggc aggtgcggaa aaagcggatg
    18421 acaatttatt tctgtatttc tggtgataat gccaggttgg ctgaaaaaat aacaaatatt
    18481 ttctttcagc ttgctataca ggtgaatctt gaaaagatgc caacggatga tccggaaata
    18541 aagcatgact gcctttttct actggatgaa ttcccgtcaa ttggtgctgt agattacatc
    18601 aagagtaaat ctggtctcat tgctggttac aagctgaaat tgttgattgt ttaccaggtc
    18661 ggctctcagc ttgaggaaat ctacagttat gcaggcagta aaacattact ggcaagtgca
    18721 ccctgcaaaa ttgtttattc cgcttctgat gttaaagatg cgcgggagtt atcagaagcg
    18781 atggggacac gaacagtaac tatcggttcg aaaagcaaaa gtcgtagtcg tggaggaaca
    18841 tcgagatctg aatcagaaag ccttattgag cgtccactgg taacaacaaa tgaactactt
    18901 acactgaaat ttagtgaaga aatcctggca atgaagggag aaaacccgat tcgttgtgaa
    18961 aaggcattct actatctcaa tgagtatttc tttggtgact ttgtgaaggt tgctccggaa
    19021 cttgcaaaca ttttccccag gaagaaagga aagctggtta tgcctccaca gaaagtattt
    19081 gaaaatgaaa tcgtcgcaaa agggtatctg gtagtaaaag atgttcctga tctcgataaa
    19141 gcggcataaa ggagaacatg atgaagaagt tagtgttttt attgatttcc attcttgctg
    19201 gttgttcatc accgccagag ccaacacccg tgcagtttga aaaggcaaat gaagtgatca
    19261 atccctcatt gccatatgtc cccgattttc acggtgtcat aaagtcagac gttagcggta
    19321 aaggatgggt ttatgagatc acttccttat ccggagtaca ggccagaacg ccgacgtttt
    19381 attacgctct ggctcatgcg gacaggattg tagtgacaac acatgatgct gggctgtggt
    19441 ttcgaatcag agatctgttg aagatggaag gggcaacagc agtcgtggaa tggcgaaacg
    19501 agaaaagttt tttacctgaa caggtaaaaa tagttttcat taaagcacaa aatgaggtga
    19561 aaaatgactg gaaaaaatag agtggtttta ccagccttgc tggcgttagg attaatatca
    19621 acaaatgcta atgcatctga cccttgcgct tcggtacttt gcttatatgg aaaagcagtc
    19681 gggcaaggtg gagggagtga atgcagaagt gctgaaaagg atttttttaa tattctgaaa
    19741 aagaaaaaag gaagcattcg ctggagtaaa acttttgatg cccgtaaagc atttctgaat
    19801 cagtgttcaa cggctgatcc tgctgctata tcaaaaatca tgagtaagtt tggtcgtgtc
    19861 agaggttaat taaaacctga taaccgcgcc aacgggtatc agggtactgc aagtatcaca
    19921 tttcgcgcca acgaaatgtg atgattagtt acaatcactg aggttagaaa tgaaagtaac
    19981 caaatctatt atagctattt tattgtgctt atctgctggt agtgcataag tggatgtaat
    20041 aaaaaatgct cgtgcctttt tgaaaggaac cctcgatctg agcgttcagg aagcagatga
    20101 agatatagag gaaagggaag aactttataa aaagaacggg gcacaacctg actatttaag
    20161 ttacctggag gattaacaca tgcaatatgc attgtttgat ggatttgaac gaaagttttt
    20221 gctggatgct cttgaattcg gtgttctgaa ggactggaaa gaaaatccgg taaaagaact
    20281 tcctgatatt gatgaatctg ctcacccctt ccatatctgt tatggtggat atttattaaa
    20341 ccctggtgtt tcagattcag atattagcag aaaaataaaa gaccaggcag gattctggct
    20401 ggcagctatt gatgataccc gcatggattg tcattcaata gcttattatg atattcacac
    20461 actccctctg atttcgtgtg gtcatcaaaa gatagttcct tttgccgcgt taataaaagc
    20521 tgatgaatgc atcatttcaa aaattgcttc gtattctggt tttgctgtaa cagccttttt
    20581 gagaattaaa gagtgggata ttgcaactaa tatacttaac cgtgagggaa tttttgcctt
    20641 taatggctgt gagcgcagat tcagagtagt aagtaaagat aactggcaac atacagtatc
    20701 ggaagaacgc gctatccgtt gtgccaaaag attaattcaa tgtaaaggat aacaaaatga
    20761 gactttttat cgcagaaaaa cccgcagtag caaatgatat tgttaaggca cttggtggca
    20821 attttacccg ccatgatggc tggtttgaaa gtgataacgc cattgtgact aactgttttg
    20881 gtcatattat cgaatcacaa ccgccggaaa actataatcc tgaatacaaa gcctggaagg
    20941 ttgaaacgct tcctttacgt ctttatcccg tgaagtatca gcctgttgaa agtgcagcaa
    21001 aacaggttaa aacgattctc gaacttatca gacgtggaga cgtgactgaa attgttcacg
    21061 ctggcgatcc tgatgatgag ggacagctac tggttgatga agtcctggaa tatgcaggca
    21121 acacaaaacc cgtaaagcgc gttctgatta acgacaacac gcttccggca gtgaaaaagg
    21181 cactggcaaa tcttaaagac aatcgtgatt tcaaaggact ttaccttaag gcgttggcgc
    21241 ggtcagttgc cgatgccgtc tatggcttct ctatgacacg tgcgtacacc attccggcaa
    21301 aagccagagg atatcagggc gttctgtctg tcgggcgcgt ccagactccc gttcttggcc
    21361 tgattgtgaa tcgtacccgt gctaaccaga accataaatc cagtttttac tacaccatga
    21421 ccggagtctt tcagcgtggt gctgatgtta tcagggcgaa ctggaagcca ggggaatttg
    21481 ctccgctgac agaccgtaaa ttacttgata aagcgtgggc agacggaacg gctgcatccc
    21541 ttgcaggaaa accggctaca gttgaagcag cagcaactga tgataaaaaa acggctgcgc
    21601 cgttgccgtt taatctggtc agactccagc aatacatgaa caagaagttt aaaatgacgg
    21661 cacaaaaaac gctggatatt acgcaacaac tacgcgaaaa atataaagca attacgtata
    21721 accgctctga ctgctcatat ctttcagatg aacaattcag cgaagcgccg caggttatcg
    21781 atgccctgaa atcagtcttt cctcagtcgc tggatattga ttctgcacgt aaaagcaaag
    21841 cgtttaacag tgcaaaggtg actgcgcata ctgcgataat cccgaccgcc agtgtgcctg
    21901 atgttaacgc actcagcacc gacgagcgca atgtttacct ggcgatcgca caacactatc
    21961 ttgttcagtt catgcctgaa aaagcatacc aggaagtatc ggttgccatt cagtgtggtg
    22021 atgagtcgtt ctatgcccgt gccagaaaaa caactgacag cggatttgag gcgtttcttg
    22081 gcgcggaaac cacagacgaa ggtgaatcag aagataatga tgattccgct tttgaactgc
    22141 tctgtaaaat tcgcacagga gaaacactga cgacaaaaga agttattgtt aatgagaaga
    22201 aaacaacacc gccgccgtta ttcaccgaag cctccttgct tgctgcgctt gttcgtgtcg
    22261 cggattttgt cactgatcca acaattaaaa aattgttgaa ggataaggat aaagacaaaa
    22321 aagatgaaca tggtggcatt ggtacgccag ctacccgcgc agccattctg gaaacgctga
    22381 agaaaagaaa ctatatcacg ctggaaaaag ggaaacttat tcctactgat accggatatg
    22441 cgcttattga tgccctgcct ggtatagcgg ttaatcctga tatgacagca ttatggtctg
    22501 aaaagcaggc tgccatagaa aatggcgacc tgacggttga acagtttatt aatgatctgt
    22561 acggtgaact gacaggcatg atttctgatg ttgacctggg cgagatgaag attgaacccg
    22621 ctgcgccagc agggcagttt caacgcctgg actctccctg cccttcctgt ggtaaacata
    22681 ttgttatcag gccgaaaggt tatttctgta ccggatgtga atttaaaatc tggagtgagt
    22741 tttctggtaa gaaaatcacc caggcacagg ccgaaaaact ggttaaatca gggaaaaccg
    22801 atttgattaa gggatttaaa aagaaaagtg gtggaacgta tgatacagtt cttgtccttg
    22861 aggataagaa aacagggaag ctgggttttc cggcaagggc taagaagtga aaacaaagca
    22921 ggaatggctt tttcagttaa gaaaatgtac atcaagagat actcttgaaa aagttattga
    22981 gattaaccgt tacaaactgc ctttatcaga atcagaggca ttttattctg ccgcagatca
    23041 tcgccgagca gaactggtga tgaataaact ttatgataag gtgccttccg gcgtctggaa
    23101 gtacgtacac taaataagag gattaattat atgagcgaac tgactaaaga agatgaatac
    23161 ggcattatca gccgtactat gatgaatatt cgttcattgc gtgtttttgc ccgtgagatt
    23221 gattttgagc agttgctcga aatgcaggaa aagctcaacg ttgttattga agaacgtcgt
    23281 gaagatgctg aacgtgaagc ggctgaacgc caggaacttg aagccaaacg tcagcaggcc
    23341 attgaataca tcatatcttt aggtcttgat cccgaatcac tccttgctcc tgtaacagca
    23401 gatacagtaa aaaccaggcg gaaagccaaa ggcggagtaa gaaaggctaa ataccgcttt
    23461 aaggatgaaa atggcgagat cagagaatgg tctggtaatg gcaagcgtcc gcttgcttta
    23521 cagaagctgc ttgatgaggg gcatttcatg gaagatttcc tcattgagaa aactaagccg
    23581 gaacaggcag aataacccgc caagtataca tcacacaggg cactgttgca aatagtcggt
    23641 ggtgataaac ttatcatccc cttttgctga tggagctgca catgaaccca ttcaaaggcc
    23701 ggcattttca gcgtgacatc attctgtggg ccgtacgctg gtactgcaaa tacggcatca
    23761 gttaccgtga gctgcaggag atgctggctg aacgcggagt gaatgtcgat cactccacga
    23821 tttaccgctg ggttcagcgt tatgcgcctg aaatggaaaa acggctgcgc tggtactggc
    23881 gtaacccttc cgatctttgc ccgtggcaca tggatgaaac ctacgtgaag gtcaatggcc
    23941 gctgggcgta tctgtaccgg gccgtcgaca gccggggccg cactgtcgat ttttatctct
    24001 cctcccgtcg taacagcaaa gctgcatacc ggtttctggg taaaatcctc aacaacgtga
    24061 agaagtggca gatcccgcga ttcatcaaca cggataaagc gcccgcctat ggtcgcgcgc
    24121 ttgctctgct caaacgcgaa ggccggtgcc cgtctgacgt tgaacaccga cagattaagt
    24181 accggaacaa cgtgattgaa tgcgatcatg gcaaactgaa acggataatc ggcgccacgc
    24241 tgggatttaa atccatgaag acggcttacg ccaccatcaa aggtattgag gtgatgcgtg
    24301 cactacgcaa aggccaggcc tcagcatttt attatggtga tcccctgggc gaaatgcgcc
    24361 tggtaagcag agtttttgaa atgtaaggcc tttgaataag acaaaaggct gcctcatcgc
    24421 taactttgca acagtgcctc acacagacat acacataggt atgtctgtgt gacataccta
    24481 tgtacagtgc cagatggcgt tgctacgtga aaaataacct accctacccc tgcttttttc
    24541 gcagatttta cgtagcacac cgttgctata gcattcatct aaaaagctga atgtcctttt
    24601 ctgtcgctgt tgcattttaa ttcactgaaa aatattgagt aatgaaaatg ccggtaacgc
    24661 gtcaagctgt tggccaacgc gacaggcagt attggaagca acaaaaaaaa tgaaagaaaa
    24721 atccctcaaa tgaaggagaa aaaacggaac tacagccgaa cgacaggcac aaaaaaaccc
    24781 gactggtaat cgggcttttt tgcgttttcc agtcttgagt tggtggctct gaccggagaa
    24841 ggttactcta cacctaacgt ttacatttta ttcactatgg tgctgtaaag caactaaatg
    24901 tggttaagga accacattta gtggtgtaac gggtgttttg tgtagcgtat aatcagtcag
    24961 ttagtcatgt agttggcttg caatcctgct gttcccgtat ctgcgggtat gaacgatttt
    25021 aaagtcctgg ctggataaac aggtggtgct gtattcggtg ctctggcagg aggaggtcac
    25081 tctacaccta acaatgcgag agagccgttg tgacaggcag tgtatgagca agcaacggtg
    25141 atttcctcca gttttactgg agaaacggat gattaatatg ttaatcatcg ttctcagagc
    25201 tgtggtcgcc gttgcaaacg cgctgattgc agttctggaa ctgatccgcc aattcatcga
    25261 ttgatgacaa cggaaacgta actaagggca ggaagtgaac cgcttcctgc cctttaacaa
    25321 aaaaggagga tttatggatt ttatgagcat tttggggcgc tgtttacttc ttcctttccc
    25381 tgttgcgatg tttgtcggtt gcctgtttcc tggcatcgat gatcgcttat ccggaatgct
    25441 tatgagtgtt gctgtgggat gtttatcgtg gtatgtcacc agaccgaaga accgacagac
    25501 aaaggccgcg taagtggcct ttaattggga taccctttag ttacaacatt ctaatagctc
    25561 attccatcct ccgctcatcc agcaaacata acctccctgc ccctccccac tacagaaagt
    25621 ggcgcgcccc gcccgtacgg gcggaatcta atcgcttaat tttgaatgtt gtaactaaac
    25681 atactcattg ctgtattgcc acaaggtttc gcagcaaggg aggataccga aagtaacgct
    25741 gtcacttttg ctttcaagtg accaaccaac gggtacatca cactgacata caataatgta
    25801 tttcttcatg atgtacatca ttgtaattca ttgtgacata cactatagta tgtccatgtg
    25861 acatacgcat gcgtacatca catagaaata cacatgagta aatcactgtg acatacaagg
    25921 ggtactatga ttatcgtaat cggtggtgat aaaggcggta caggtaaatc acatctggca
    25981 accaacttaa ctgtttgcct ctcacaacag ggaaagagaa cggggcttgt tgaaacagac
    26041 ctgaatggct cgaccaaaaa atggaataaa cgcagagaac aggccggatt accgcccgtc
    26101 gcattaaatg aagcctatgg cgacatcagt gccaaaataa caaaaatggc agatgtagca
    26161 gaaatcctca ttatagatac tgccggatat gacagcacgg agttcagaac tgcactaaaa
    26221 gttgcagaca ttgtgattgt cccgatcgac ccgcttgccc aggtggaagc agacagtctt
    26281 cagactgtga caaaaattgt cagggatgcg caaaaaataa atcccaggct ggctgcacat
    26341 gtgctcctgt ataaatgcca gccaaatacg ttcagtgaac aacaagaatt aagagactct
    26401 ctcaacagcc atgattactg gcttaaacca atgaaaagca cggtttcttt tctccgggcc
    26461 tttgtaagag caatgaatca gggaatgggg gttcacgagt taaaaagtaa tgttagcggt
    26521 gccagccagg caaaagcaca aatcgaactc ttgctgaaag aactggaact gtaaatcata
    26581 gtgacataca ctaatgtaca tcatcatgat atacattagt gtgaaacaat cagattagaa
    26641 ggtacatcat ggcaaagctg gaactaccag aactagaaca aaaccaggaa gtgaacgcca
    26701 caaaatttgt tcaggacgca ggaaaacgcc ccacagagaa caaaacaaaa ctggcttcat
    26761 tccgcatacc gaatgaattg cttgaattca ttgataacga atcaaaaaga ctgcatcgtc
    26821 ccaagacaaa aataatcaaa gcagcattac tggcttatca ggatctggac gaaaacgaac
    26881 tcattaattt gtggttcaga gctgacagaa atgattaatc atagcaagtg gcccaataaa
    26941 ctctttatca ttttctttta gatacttatc tgaatgtact aacttcatca cttttaaatg
    27001 tagttgtgat tcatttttag ttactcttgc aataaaatac ccttgtacag ttgttgcacg
    27061 ggtatttagg agcactagat gagccttatc gatttaagtt tatcaggatt atcagaacca
    27121 ggaacaaaac ttattgaaaa aataagtgat gctattggtg tgctttatga acctacaaga
    27181 atcaggaaaa aagcgaaggc tgaagctgaa gctaaacgca cagagctaat ttctaggtta
    27241 gagcttgagg gaatagaaaa acgggcagtt gagcgttttc tcaaacgtga aacgaaacga
    27301 caggaaaaca ttgaaaatat aacaatgcaa gcagcacaaa gtctatctga gagtgataat
    27361 gtttctgata ttgacgaaga ttggatagag gctttcttca gggagtgtga ggatattagt
    27421 gacgaacaaa tgcaaatgct ttggggaaga attttatccg aagaagcaaa gtcaaaaggt
    27481 tcgtttagtc gtagaacctt aaaactatta tccactataa gcaaagaaga agcgaacctt
    27541 attacttatt ttggaaagtt tgtttggcag gcaaacaaac tcactccaat tttatttacc
    27601 gatgagaatg gagatactga aggtataact ttcgataaac tttcggtttt agactcctta
    27661 ggcgttattc aacaaggcat tggttatagt ttacaaatta taaatactct caagtgtata
    27721 ttcagtatgg gattgcgcaa tgattgccta ataaaatttc tgaaatattt ctgtatcgca
    27781 taatttttta tatcagataa attgtactgg atttcttaaa aaattgcagt ataattgccg
    27841 caattatccc accgtttatt ttttgagtag tttctcatga tgcagcatac ttctgtgtgg
    27901 taccgacgct cggtcagtcc gtttgttctt gtggcgagtg ttgccgtttt cttgaccgcg
    27961 accgccaatc ttaccttttt tgataaaatc agccaaacct atcccatcgc ggacaatctc
    28021 ggctttgtgc tgacgatcgc tgtcgtgctc tttggcgcga tgctactgat caccacgctg
    28081 ttatcatcgt atcgctatgt gctaaagcct gtgttgattt tgctattaat catgggcgcg
    28141 gtgaccagtt attttactga cacttatggc acggtctatg atacgaccat gatccaaaat
    28201 gccctacaga ccgaccaagc cgagaccaag gatctattaa acgcagcgtt tatcatgcgt
    28261 atcattggtt tgggtgtgct accaagtttg cttgtggctt ttgttaaggt ggattatccg
    28321 acttggggca agggtttgat gcgccgattg ggcttgatcg tggcaagtct tgcgctgatt
    28381 ttactgcctg tggtggcgtt cagcagtcat tatgccagtt tctttcgcgt gcataagccg
    28441 ctgcgtagct atgtcaatcc gatcatgcca atctactcgg tgggtaagct tgccagtatt
    28501 gagtataaaa aagccagtgc gccaaaagat accatttatc acgccaaaga cgcggtacaa
    28561 gcaaccaagc ctgatatgcg taagccacgc ctagtggtgt tcgtcgtcgg tgagacggca
    28621 cgcgccgatc atgtcagctt caatggctat gagcgcgata ctttcccaca gcttgccaag
    28681 atcgatggcg tgaccaattt tagcaatgtc acatcgtgcg gcacatcgac ggcgtattct
    28741 gtgccgtgta tgttcagcta tctgggcgcg gatgagtatg atgtcgatac cgccaaatac
    28801 caagaaaatg tgctggatac gctggatcgc ttgggcgtaa gtatcttgtg gcgtgataat
    28861 aattcggact caaaaggcgt gatggataag ctgccaaaag cgcaatttgc cgattataaa
    28921 tccgcgacca acaacgccat ctgcaacacc aatccttata acgaatgccg cgatgtcggt
    28981 atgctcgttg gcttagatga ctttgtcgct gccaataacg gcaaagatat gctgatcatg
    29041 ctgcaccaaa tgggcaatca cgggcctgcg tattttaagc gatatgatga aaagtttgcc
    29101 aaattcacgc cagtgtgtga aggtaatgag cttgccaagt gcgaacatca gtccttgatc
    29161 aatgcttatg acaatgcctt gcttgccacc gatgatttca tcgctcaaag tatccagtgg
    29221 ctgcagacgc acagcaatgc ctatgatgtc tcaatgctgt atgtcagcga tcatggcgaa
    29281 agtctgggtg agaacggtgt ctatctacat ggtatgccaa atgcctttgc accaaaagaa
    29341 cagcgcagtg tgcctgcatt tttctggacg gataagcaaa ctggcatcac gccaatggca
    29401 accgataccg tcctgaccca tgacgcgatc acgccgacat tattaaagct gtttgatgtc
    29461 accgcggaca aagtcaaaga ccgcaccgca ttcatccgct gatttctccc tgtatttttt
    29521 ccaaacccac cgcacactcc attcgtatta tgggcggtgg ggtggggttt gttatgccgt
    29581 atttatcaaa taaacgccta cttgctgaga tgagtatcgc tcttgtcatg gcgatcgttg
    29641 ccacgctgac ccttgagcac agtcagattg atctgatggt cgctgattgg ttttatctgg
    29701 gtatggggca ttggatggtt gccaagcaag cttttttgcc agatttgcta ctgtattctg
    29761 gactaaaaaa gctgctgatg gcgatgctga tctacttgct ggttgcgacc atttgccgtg
    29821 cttatcatga gaaaaagggc aatgctatca ctgccaagtg gcttgtccca gtgacaaaat
    29881 ttcgcgtgcg tgagcttgcg tatctggtgc tgactttgat cctagtgccg acagttgtcg
    29941 cgtcattgaa ggcatatact catgtggtct gccctgtgca tttgacgatt tttgatggta
    30001 cgctgccgta tttgccgatg cttgatagta tgcgtaacac cattcctgat aagtgctttc
    30061 ctgcggcgca tgccagtagc ggatttgcgc tgtttgcctt tgcgtttgcg ccaagtttgc
    30121 gccgccgtcg tggtgcgatc atcatcgtgg tgatggcatt gggctgggcg atgggctgct
    30181 ataagatgat tattggcgat cattttttga gccatacggt ggtgtcgatg atgcttgcgt
    30241 gggcgatgtc ggcagggctt gcgtgggtgt tttttaagaa gggtgaacaa gtttaatcgc
    30301 ctttaaaagt aaaaacggtc gtatcggtta ttacggaacg gttttttcag ttgagtttca
    30361 aagcgatgat tcatcaacct ggaaactacc aataggaacg gctttactta cgcctatcgg
    30421 tcaagaattg atgaaaatat gcggttccac tccagatatt gcgtatttaa ataaattcct
    30481 caacaaaata aatgttgaag gaagccaggt aaaactatca attattaata tgtaaagagg
    30541 tcgagttacc tgtctgagtt ccactaacca taattaaaag cgaccgcaaa aactgcccag
    30601 tgaaaactgt gttgccagta atcacaaatg cagcaagtac taataaagag cctgttaagc
    30661 ccgcaaatat gtgggcttat ttttaattac tcttcatcat cccgcaggaa ctgacaaaaa
    30721 tcttcgctcc ccatcatatc gacagtgttt ttaaattcga aaaaagaaat tcctgtcata
    30781 gcacacacgc gctggataat cagttttttc ttttgctgtc tgacggttgc ggtttcacca
    30841 aactggataa cagccaggac tgtttcatca tatgatttca ggacaaaatc acaggttata
    30901 ttctgcactt cacggattaa atcttttaca gcttcctcgc taccatgagg acgaacaaat
    30961 tcaatgagcc gtattttggg aaaaacggaa taatcaggta taatattacg tgaaagaatg
    31021 cctaaaaact ctgattcatt atttgagagt aagggtactt tagtaaaaca atcaacctcc
    31081 ccaatggctg taaaagcatt ggttgtcaaa gctggattgt tcttaataaa atcagagaat
    31141 acattttcag ctgatggcaa aacaacagca gaattatgtt gttcatctcg ttttttatta
    31201 cggttaaata tcatttcata ttccttttaa aagagcctgc ggcaggcaca ggcaaaaaca
    31261 tcgtttttgg acaatctgat tcaaatgtta acatttgaca tcggattgtc tatcctgcat
    31321 tgttctttac tgtctgtaag atcggcgctc attcttccgg cgttctcccg ataccaaaaa
    31381 gacggaacgc aaaacggctt tcgatccacc attcaaaaat ctgctctgcg caaccacaaa
    31441 cagttaaggc gatccatgca gcaagaacaa cgtaactact gacatctgca cccatgtacc
    31501 aggcgatagt taaacccatc actgcgatac cgcctaaaaa ctgcaacaag aaacgaaatc
    31561 ccgcaagaat ccccaatgga ataacaacaa ccagataaag ggaagcagcc agaaggtatc
    31621 tgacaggtgt cagcagataa aagaacctgg atattttctt tttgtcagaa taaacggcat
    31681 catttgcaac atcggttgaa tgatcattca tgctgtccct gattctttga aaatctacga
    31741 tattatcctt catattgtta tgacctcaat tttcaggtgg gtacatttta tcaaacatat
    31801 tagtaaactc tgagtcttcc ctggtatcgc cctcataacc aattgtataa gagaacttta
    31861 atttataaac ttttctccca attttttctg aaactacgaa acttaagttc tttacttccg
    31921 tatgtttcat gatttctttt actgatttat taagaacatc tcttttaaaa gcaggataat
    31981 ctggatactt ataatccttc accccccccg cccctattgt ataaagattc aattcatctt
    32041 taagttcatc tattgtcatt tcaaaactat tcgcgcgaga attattacta tatattttac
    32101 gaatgagttg atatagtgat gcagcattaa cacttgtcag tcgaacaaca gataataaaa
    32161 cttgcgttgt ataacgtttt tcttcaccaa taagtttaca taaaaatctt ttcgcttctt
    32221 tcgtaaactt gatccccact ctaccttcat tcctgtaata agtagaaaaa acagtcagac
    32281 tcaaattcat tgaatcaggt acattacttt tagtgaactt aaatcccaaa tcactactca
    32341 gttctaatat ttcatcatgt ttcagcttaa gaagcgtcgt atctaatata ttagagccat
    32401 ccctcaaagc aagataagcc gcatcaggct taacctgaac ccatttaatg taatcggcaa
    32461 ctgtcacata aaatatgtga tcatcatcga attcattttt agagtttatc tggcacatgg
    32521 ctaaaaataa gactttttta gcagccatag gcaatgttga cagcgttgaa ttcaattcat
    32581 ttcgatgtct cacttttgtt aagcgagaaa gggaagtctt atttgtcgtc attctcattg
    32641 ttccaacact cttgataatc agactttaac accagaaaca tgatattaca acacaatcaa
    32701 tatcatgttt aaccgagtat cttctcatgt ttcatatctg tgatttatct cacaaaggta
    32761 atccatgctc aaaatccaca ctggattcaa ggcagattaa caacagattc aaaatagatt
    32821 caccagaaag aacaacgaat tagtattctt atgacacatt ttgtggatat caccgaggaa
    32881 gctctcatgt ttaaccgagg aagctctcat gtttaaccga ggaagctctc atgtttaacc
    32941 gaggaagctc tcatgtttaa ccgaggaagt tatcatgttt aaccgaggaa gctctcatgt
    33001 ttagacaaaa aacacgctta aaatcaataa attagagcta ctctaagttt ttaagttttt
    33061 aagaaaaaca agaaaagata aggcttttaa tgcgtgcgaa tattcaggaa ctgtggataa
    33121 ctcaacaaaa ccagcaacga tgatggttcc ttaccataaa tctgtccccc gatgattatt
    33181 acccgcaagc taaaacaaac atcatcaatc attaaaactc ataactttca ataacataaa
    33241 atcatcaaaa aaatatttga tgccttatgt atctcaattt tcaagaccat aaatttgtcc
    33301 cta
//
