LOCUS       NZ_OR724972            34639 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Escherichia coli strain Hum1 plasmid pHum1, complete sequence.
ACCESSION   NZ_OR724972
VERSION     NZ_OR724972.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34639)
  AUTHORS   Nordhoff,K., Scharlach,M., Effelsberg,N., Knorr,C., Rocker,D.,
            Claussen,K., Egelkamp,R., Mellmann,A.C., Moss,A., Muller,I.,
            Roth,S.A., Werckenthin,C., Wohlke,A., Ehlers,J. and Kock,R.
  TITLE     Epidemiology and zoonotic transmission of mcr-positive and
            carbapenemase-producing Enterobacterales on German turkey farms
  JOURNAL   Front Microbiol 14, 1183984 (2023)
   PUBMED   37346748
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34639)
  AUTHORS   Effelsberg,N., Mellmann,A.C. and Kock,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2023) Next Generation Sequencing,
            Niedersaechsisches Landesgesundheitsamt, Roesebeckstrasse 4-6,
            Hannover, Lower Saxony 30449, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4; SMRT Link v. 8.0
            Assembly Name :: mcr1-encondig plasmids found in Escherichia coli
            Sequencing Technology             :: PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 10:46:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 41
            CDSs (total)                      :: 41
            Genes (coding)                    :: 39
            CDSs (with protein)               :: 39
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OR724972.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..34639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Hum1"
                     /serotype="O117:H4"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pHum1"
                     /geo_loc_name="Germany: Lower Saxony"
                     /collection_date="2018-05-14"
                     /collected_by="Public Health Agency of Lower Saxony (NLGA)"
                     /note="mcr1-encoding plasmid"
     gene            complement(join(34230..34639,1..106))
                     /locus_tag="TU555_RS00005"
                     /old_locus_tag="pHum1_000001"
     CDS             complement(join(34230..34639,1..106))
                     /locus_tag="TU555_RS00005"
                     /old_locus_tag="pHum1_000001"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            1521..2387
                     /locus_tag="TU555_RS00010"
                     /old_locus_tag="pHum1_000003"
     CDS             1521..2387
                     /locus_tag="TU555_RS00010"
                     /old_locus_tag="pHum1_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            2400..2804
                     /locus_tag="TU555_RS00015"
                     /old_locus_tag="pHum1_000004"
     CDS             2400..2804
                     /locus_tag="TU555_RS00015"
                     /old_locus_tag="pHum1_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            2939..3466
                     /locus_tag="TU555_RS00020"
                     /old_locus_tag="pHum1_000005"
     CDS             2939..3466
                     /locus_tag="TU555_RS00020"
                     /old_locus_tag="pHum1_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(3857..4603)
                     /locus_tag="TU555_RS00025"
                     /old_locus_tag="pHum1_000007"
     CDS             complement(3857..4603)
                     /locus_tag="TU555_RS00025"
                     /old_locus_tag="pHum1_000007"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_077248209.1"
                     /translation="MGGGVGFVMPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVA
                     DWFYLGMGHWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKW
                     LVPVTKFRVRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRN
                     TIPDKCFPAAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSH
                     TVVSMMLAWAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(4651..6276)
                     /locus_tag="TU555_RS00030"
                     /old_locus_tag="pHum1_000008"
     CDS             complement(4651..6276)
                     /locus_tag="TU555_RS00030"
                     /old_locus_tag="pHum1_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            complement(6370..7074)
                     /locus_tag="TU555_RS00035"
                     /old_locus_tag="pHum1_000009"
     CDS             complement(6370..7074)
                     /locus_tag="TU555_RS00035"
                     /old_locus_tag="pHum1_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_225236207.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLQIINTLKCIFSMGLRND
                     CLIKFLKYFCIA"
                     /name="DUF2806 domain-containing protein"
     gene            complement(7235..7504)
                     /locus_tag="TU555_RS00040"
                     /old_locus_tag="pHum1_000011"
     CDS             complement(7235..7504)
                     /locus_tag="TU555_RS00040"
                     /old_locus_tag="pHum1_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            complement(7579..8226)
                     /locus_tag="TU555_RS00045"
                     /old_locus_tag="pHum1_000012"
     CDS             complement(7579..8226)
                     /locus_tag="TU555_RS00045"
                     /old_locus_tag="pHum1_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            complement(8889..8984)
                     /gene="dqlB"
                     /locus_tag="TU555_RS00050"
     CDS             complement(8889..8984)
                     /gene="dqlB"
                     /locus_tag="TU555_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            complement(9767..10471)
                     /locus_tag="TU555_RS00055"
                     /old_locus_tag="pHum1_000016"
     CDS             complement(9767..10471)
                     /locus_tag="TU555_RS00055"
                     /old_locus_tag="pHum1_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(10558..11022)
                     /locus_tag="TU555_RS00060"
                     /old_locus_tag="pHum1_000017"
     CDS             complement(10558..11022)
                     /locus_tag="TU555_RS00060"
                     /old_locus_tag="pHum1_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            complement(11040..11246)
                     /gene="hha"
                     /locus_tag="TU555_RS00065"
                     /old_locus_tag="pHum1_000018"
     CDS             complement(11040..11246)
                     /gene="hha"
                     /locus_tag="TU555_RS00065"
                     /old_locus_tag="pHum1_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            complement(11243..13396)
                     /locus_tag="TU555_RS00070"
                     /old_locus_tag="pHum1_000019"
     CDS             complement(11243..13396)
                     /locus_tag="TU555_RS00070"
                     /old_locus_tag="pHum1_000019"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            complement(13401..13973)
                     /locus_tag="TU555_RS00075"
                     /old_locus_tag="pHum1_000020"
     CDS             complement(13401..13973)
                     /locus_tag="TU555_RS00075"
                     /old_locus_tag="pHum1_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            complement(13977..>14171)
                     /locus_tag="TU555_RS00080"
                     /pseudo=""
     CDS             complement(13977..>14171)
                     /locus_tag="TU555_RS00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            complement(14284..14589)
                     /locus_tag="TU555_RS00085"
                     /old_locus_tag="pHum1_000021"
     CDS             complement(14284..14589)
                     /locus_tag="TU555_RS00085"
                     /old_locus_tag="pHum1_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            complement(14573..14995)
                     /locus_tag="TU555_RS00090"
                     /old_locus_tag="pHum1_000022"
     CDS             complement(14573..14995)
                     /locus_tag="TU555_RS00090"
                     /old_locus_tag="pHum1_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(15004..16866)
                     /gene="taxB"
                     /locus_tag="TU555_RS00095"
                     /old_locus_tag="pHum1_000023"
     CDS             complement(15004..16866)
                     /gene="taxB"
                     /locus_tag="TU555_RS00095"
                     /old_locus_tag="pHum1_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            complement(16853..17881)
                     /gene="virB11"
                     /locus_tag="TU555_RS00100"
                     /old_locus_tag="pHum1_000024"
     CDS             complement(16853..17881)
                     /gene="virB11"
                     /locus_tag="TU555_RS00100"
                     /old_locus_tag="pHum1_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            complement(17883..19001)
                     /gene="virB10"
                     /locus_tag="TU555_RS00105"
                     /old_locus_tag="pHum1_000025"
     CDS             complement(17883..19001)
                     /gene="virB10"
                     /locus_tag="TU555_RS00105"
                     /old_locus_tag="pHum1_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            complement(18994..19902)
                     /gene="virB9"
                     /locus_tag="TU555_RS00110"
                     /old_locus_tag="pHum1_000026"
     CDS             complement(18994..19902)
                     /gene="virB9"
                     /locus_tag="TU555_RS00110"
                     /old_locus_tag="pHum1_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            complement(19899..20585)
                     /locus_tag="TU555_RS00115"
                     /old_locus_tag="pHum1_000027"
     CDS             complement(19899..20585)
                     /locus_tag="TU555_RS00115"
                     /old_locus_tag="pHum1_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            complement(20924..21073)
                     /locus_tag="TU555_RS00120"
                     /old_locus_tag="pHum1_000030"
     CDS             complement(20924..21073)
                     /locus_tag="TU555_RS00120"
                     /old_locus_tag="pHum1_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227648764.1"
                     /translation="MSLNWILVFVDKHITSGWSRKRKLITQLVILITGITLFKVLGGIS
                     QSWL"
                     /name="hypothetical protein"
     gene            complement(21100..22194)
                     /locus_tag="TU555_RS00125"
                     /old_locus_tag="pHum1_000031"
     CDS             complement(21100..22194)
                     /locus_tag="TU555_RS00125"
                     /old_locus_tag="pHum1_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            22409..23637
                     /locus_tag="TU555_RS00130"
                     /old_locus_tag="pHum1_000033"
     CDS             join(22409..22724,22724..23637)
                     /locus_tag="TU555_RS00130"
                     /old_locus_tag="pHum1_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_089541817.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKTAVPPSKR
                     AHTGKVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFDSETVQ
                     DVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQASN
                     GLSDNRCLEI"
                     /name="IS3 family transposase"
     gene            complement(23608..24258)
                     /locus_tag="TU555_RS00135"
                     /old_locus_tag="pHum1_000034"
     CDS             complement(23608..24258)
                     /locus_tag="TU555_RS00135"
                     /old_locus_tag="pHum1_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_320443626.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYVDLPLYFQTPVIT"
                     /name="type IV secretion system protein"
     gene            complement(24270..27017)
                     /locus_tag="TU555_RS00140"
                     /old_locus_tag="pHum1_000035"
     CDS             complement(24270..27017)
                     /locus_tag="TU555_RS00140"
                     /old_locus_tag="pHum1_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            complement(27036..27362)
                     /locus_tag="TU555_RS00145"
                     /old_locus_tag="pHum1_000036"
     CDS             complement(27036..27362)
                     /locus_tag="TU555_RS00145"
                     /old_locus_tag="pHum1_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            complement(27376..27995)
                     /locus_tag="TU555_RS00150"
                     /old_locus_tag="pHum1_000037"
                     /pseudo=""
     CDS             complement(27376..27995)
                     /locus_tag="TU555_RS00150"
                     /old_locus_tag="pHum1_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(28351..28566)
                     /locus_tag="TU555_RS00155"
                     /old_locus_tag="pHum1_000038"
     CDS             complement(28351..28566)
                     /locus_tag="TU555_RS00155"
                     /old_locus_tag="pHum1_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            complement(28563..28856)
                     /locus_tag="TU555_RS00160"
                     /old_locus_tag="pHum1_000039"
     CDS             complement(28563..28856)
                     /locus_tag="TU555_RS00160"
                     /old_locus_tag="pHum1_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(28933..30162)
                     /gene="taxC"
                     /locus_tag="TU555_RS00165"
                     /old_locus_tag="pHum1_000040"
     CDS             complement(28933..30162)
                     /gene="taxC"
                     /locus_tag="TU555_RS00165"
                     /old_locus_tag="pHum1_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            complement(30166..30669)
                     /gene="taxA"
                     /locus_tag="TU555_RS00170"
                     /old_locus_tag="pHum1_000041"
     CDS             complement(30166..30669)
                     /gene="taxA"
                     /locus_tag="TU555_RS00170"
                     /old_locus_tag="pHum1_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            complement(31030..31377)
                     /locus_tag="TU555_RS00175"
                     /old_locus_tag="pHum1_000043"
     CDS             complement(31030..31377)
                     /locus_tag="TU555_RS00175"
                     /old_locus_tag="pHum1_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            complement(31374..31622)
                     /locus_tag="TU555_RS00180"
                     /old_locus_tag="pHum1_000044"
     CDS             complement(31374..31622)
                     /locus_tag="TU555_RS00180"
                     /old_locus_tag="pHum1_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            complement(31927..32277)
                     /locus_tag="TU555_RS00185"
                     /old_locus_tag="pHum1_000045"
     CDS             complement(31927..32277)
                     /locus_tag="TU555_RS00185"
                     /old_locus_tag="pHum1_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            complement(32368..32586)
                     /locus_tag="TU555_RS00190"
                     /old_locus_tag="pHum1_000046"
     CDS             complement(32368..32586)
                     /locus_tag="TU555_RS00190"
                     /old_locus_tag="pHum1_000046"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005058042.1"
                     /translation="MHKDTGQQYVVIFTDNNKIRDYKAGIVPQFGELKQSDVDLVLFYR
                     DEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            complement(33121..33348)
                     /locus_tag="TU555_RS00195"
                     /old_locus_tag="pHum1_000048"
     CDS             complement(33121..33348)
                     /locus_tag="TU555_RS00195"
                     /old_locus_tag="pHum1_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            complement(33335..33586)
                     /locus_tag="TU555_RS00200"
                     /old_locus_tag="pHum1_000049"
     CDS             complement(33335..33586)
                     /locus_tag="TU555_RS00200"
                     /old_locus_tag="pHum1_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            complement(33583..34125)
                     /locus_tag="TU555_RS00205"
                     /old_locus_tag="pHum1_000050"
     CDS             complement(33583..34125)
                     /locus_tag="TU555_RS00205"
                     /old_locus_tag="pHum1_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     ARG             complement(4655..6276)
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1516..2387
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(28937..30162)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(15008..16866)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(18998..19902)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19903..20585)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          complement(21128..22194)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.6999999999999997e-172"
ORIGIN
        1 gatctggatg atattttaaa gcggcttttc tgtatgctgc tttgatatct ttttcagtta
       61 attcgccgga taatccaaaa acgtttaatg cttcctggat attcattttt taaatctcct
      121 gccctgctgg gctattgtga gaggggccat tcccctttcg atgaaacaat aatacgacct
      181 ttaagtcgta tttgcaagtt ttctcccgtt tttttactcg ataagtgatt gttatcacgt
      241 tttttaatcc gcgtaagcgg atggaagggg cgaacgaaaa taccagggca gcacaggagc
      301 cacatcgccc cgaagcgcgg cgcagccgct gacgaacgta ccagggtgca caggccatcc
      361 cttcaccctg caaagcccaa ctgaccagca ggccatgatt atgctgattt gcgccgcaga
      421 atgccgcagt tagcgcgagc gcaagcgagt ttaccccgcc ctggtgcatt gcagcatggc
      481 ggggcgctgt actgcgcgtg tgagcgttag cgaactacgg gcgcaatgca ggagaactaa
      541 cagaacctcc ctaaaacgct ctgaatcgca ttttaagcga tttttttcat ttcgcatggt
      601 tttcctcatg gttgtatcga acgaagcggc agagcgcgat tcagggcttc ttagtggaag
      661 atccaaaaag gatcaaataa cggatcatat gatctgaatc tctctggttt tgagaaagga
      721 gataccgctc gccgcagccg aacggcagga gcgtagcgac tgagtgagcg aggaagcgga
      781 atttttgcga tgtgacatta ggatcagtac gcacgaatat tatatttatt tcaataagtt
      841 aaagttaact agggacaaat ttatggtctt gaaaattgag atacataagg catcaaatat
      901 ttttttgatg attttatgtt attgaaagtt atgagtttta atgattgatg atgtttgttt
      961 tagcttgcgg gtaataatca tcgggggaca gatttatggt aaggaaccat catcgttgct
     1021 ggttttgttg agttatccac agttcctgaa tattcgcacg cattaaaagc cttatctttt
     1081 cttgtttttc ttaaaaactt aaaaacttag agtagctcta atttattgat tttaagcgtg
     1141 ttttttgtct aaacatgaga gcttcctcgg ttaaacatga taacttcctc ggttaaacat
     1201 gagagcttcc tcggttaaac atgagagctt cctcggttaa acatgagagc ttcctcggtt
     1261 aaacatgaga gcttcctcgg tgatatccac aaaatgtgtc ataagaatac taattcgttg
     1321 ttctttctgg tgaatctatt ttgaatctgt tgttaatctg ccttgaatcc agtgtggatt
     1381 ttgagcatgg attacctttg tgagataaat cacagatatg aaacatgaga agatactcgg
     1441 ttaaacatga tattgattgt gttgtaatat catgtttctg gtgttaaagt ctgattatca
     1501 agagtgttgg aacaatgaga atgacgacaa ataagacttc cctttctcgc ttaacaaaag
     1561 tgagacatcg aaatgaattg aattcaacgc tgtcaacatt gcctatggct gctaaaaaag
     1621 tcttattttt agccatgtgc cagataaact ctaaaaatga attcgatgat gatcacatat
     1681 tttatgtgac agttgccgat tacattaaat gggttcaggt taagcctgat gcggcttatc
     1741 ttgctttgag ggatggctct aatatattag atacgacgct tcttaagctg aaacatgatg
     1801 aaatattaga actgagtagt gatttgggat ttaagttcac taaaagtaat gtacctgatt
     1861 caatgaattt gagtctgact gttttttcta cttattacag gaatgaaggt agagtgggga
     1921 tcaagtttac gaaagaagcg aaaagatttt tatgtaaact tattggtgaa gaaaaacgtt
     1981 atacaacgca agttttatta tctgttgttc gactgacaag tgttaatgct gcatcactat
     2041 atcaactcat tcgtaaaata tatagtaata attctcgcgc gaatagtttt gaaatgacaa
     2101 tagatgaact taaagatgaa ttgaatcttt atacaatagg ggcggggggg gtgaaggatt
     2161 ataagtatcc agattatcct gcttttaaaa gagatgttct taataaatca gtaaaagaaa
     2221 tcatgaaaca tacggaagta aagaacttaa gtttcgtagt ttcagaaaaa attgggagaa
     2281 aagtttataa attaaagttc tcttatacaa ttggttatga gggcgatacc agggaagact
     2341 cagagtttac taatatgttt gataaaatgt acccacctga aaattgaggt cataacaata
     2401 tgaaggataa tatcgtagat tttcaaagaa tcagggacag catgaatgat cattcaaccg
     2461 atgttgcaaa tgatgccgtt tattctgaca aaaagaaaat atccaggttc ttttatctgc
     2521 tgacacctgt cagatacctt ctggctgctt ccctttatct ggttgttgtt attccattgg
     2581 ggattcttgc gggatttcgt ttcttgttgc agtttttagg cggtatcgca gtgatgggtt
     2641 taactatcgc ctggtacatg ggtgcagatg tcagtagtta cgttgttctt gctgcatgga
     2701 tcgccttaac tgtttgtggt tgcgcagagc agatttttga atggtggatc gaaagccgtt
     2761 ttgcgttccg tctttttggt atcgggagaa cgccggaaga atgagcgccg atcttacaga
     2821 cagtaaagaa caatgcagga tagacaatcc gatgtcaaat gttaacattt gaatcagatt
     2881 gtccaaaaac gatgtttttg cctgtgcctg ccgcaggctc ttttaaaagg aatatgaaat
     2941 gatatttaac cgtaataaaa aacgagatga acaacataat tctgctgttg ttttgccatc
     3001 agctgaaaat gtattctctg attttattaa gaacaatcca gctttgacaa ccaatgcttt
     3061 tacagccatt ggggaggttg attgttttac taaagtaccc ttactctcaa ataatgaatc
     3121 agagttttta ggcattcttt cacgtaatat tatacctgat tattccgttt ttcccaaaat
     3181 acggctcatt gaatttgttc gtcctcatgg tagcgaggaa gctgtaaaag atttaatccg
     3241 tgaagtgcag aatataacct gtgattttgt cctgaaatca tatgatgaaa cagtcctggc
     3301 tgttatccag tttggtgaaa ccgcaaccgt cagacagcaa aagaaaaaac tgattatcca
     3361 gcgcgtgtgt gctatgacag gaatttcttt tttcgaattt aaaaacactg tcgatatgat
     3421 ggggagcgaa gatttttgtc agttcctgcg ggatgatgaa gagtaattaa aaataagccc
     3481 acatatttgc gggcttaaca ggctctttat tagtacttgc tgcatttgtg attactggca
     3541 acacagtttt cactgggcag tttttgcggt cgcttttaat tatggttagt ggaactcaga
     3601 caggtaactc gacctcttta catattaata attgatagtt ttacctggct tccttcaaca
     3661 tttattttgt tgaggaattt atttaaatac gcaatatctg gagtggaacc gcatattttc
     3721 atcaattctt gaccgatagg cgtaagtaaa gccgttccta ttggtagttt ccaggttgat
     3781 gaatcatcgc tttgaaactc aactgaaaaa accgttccgt aataaccgat acgaccgttt
     3841 ttacttttaa aggcgattaa acttgttcac ccttcttaaa aaacacccac gcaagccctg
     3901 ccgacatcgc ccacgcaagc atcatcgaca ccaccgtatg gctcaaaaaa tgatcgccaa
     3961 taatcatctt atagcagccc atcgcccagc ccaatgccat caccacgatg atgatcgcac
     4021 cacgacggcg gcgcaaactt ggcgcaaacg caaaggcaaa cagcgcaaat ccgctactgg
     4081 catgcgccgc aggaaagcac ttatcaggaa tggtgttacg catactatca agcatcggca
     4141 aatacggcag cgtaccatca aaaatcgtca aatgcacagg gcagaccaca tgagtatatg
     4201 ccttcaatga cgcgacaact gtcggcacta ggatcaaagt cagcaccaga tacgcaagct
     4261 cacgcacgcg aaattttgtc actgggacaa gccacttggc agtgatagca ttgccctttt
     4321 tctcatgata agcacggcaa atggtcgcaa ccagcaagta gatcagcatc gccatcagca
     4381 gcttttttag tccagaatac agtagcaaat ctggcaaaaa agcttgcttg gcaaccatcc
     4441 aatgccccat acccagataa aaccaatcag cgaccatcag atcaatctga ctgtgctcaa
     4501 gggtcagcgt ggcaacgatc gccatgacaa gagcgatact catctcagca agtaggcgtt
     4561 tatttgataa atacggcata acaaacccca ccccaccgcc cataatacga atggagtgtg
     4621 cggtgggttt ggaaaaaata cagggagaaa tcagcggatg aatgcggtgc ggtctttgac
     4681 tttgtccgcg gtgacatcaa acagctttaa taatgtcggc gtgatcgcgt catgggtcag
     4741 gacggtatcg gttgccattg gcgtgatgcc agtttgctta tccgtccaga aaaatgcagg
     4801 cacactgcgc tgttcttttg gtgcaaaggc atttggcata ccatgtagat agacaccgtt
     4861 ctcacccaga ctttcgccat gatcgctgac atacagcatt gagacatcat aggcattgct
     4921 gtgcgtctgc agccactgga tactttgagc gatgaaatca tcggtggcaa gcaaggcatt
     4981 gtcataagca ttgatcaagg actgatgttc gcacttggca agctcattac cttcacacac
     5041 tggcgtgaat ttggcaaact tttcatcata tcgcttaaaa tacgcaggcc cgtgattgcc
     5101 catttggtgc agcatgatca gcatatcttt gccgttattg gcagcgacaa agtcatctaa
     5161 gccaacgagc ataccgacat cgcggcattc gttataagga ttggtgttgc agatggcgtt
     5221 gttggtcgcg gatttataat cggcaaattg cgcttttggc agcttatcca tcacgccttt
     5281 tgagtccgaa ttattatcac gccacaagat acttacgccc aagcgatcca gcgtatccag
     5341 cacattttct tggtatttgg cggtatcgac atcatactca tccgcgccca gatagctgaa
     5401 catacacggc acagaatacg ccgtcgatgt gccgcacgat gtgacattgc taaaattggt
     5461 cacgccatcg atcttggcaa gctgtgggaa agtatcgcgc tcatagccat tgaagctgac
     5521 atgatcggcg cgtgccgtct caccgacgac gaacaccact aggcgtggct tacgcatatc
     5581 aggcttggtt gcttgtaccg cgtctttggc gtgataaatg gtatcttttg gcgcactggc
     5641 ttttttatac tcaatactgg caagcttacc caccgagtag attggcatga tcggattgac
     5701 atagctacgc agcggcttat gcacgcgaaa gaaactggca taatgactgc tgaacgccac
     5761 cacaggcagt aaaatcagcg caagacttgc cacgatcaag cccaatcggc gcatcaaacc
     5821 cttgccccaa gtcggataat ccaccttaac aaaagccaca agcaaacttg gtagcacacc
     5881 caaaccaatg atacgcatga taaacgctgc gtttaataga tccttggtct cggcttggtc
     5941 ggtctgtagg gcattttgga gcatggtcgt atcatagacc gtgccataag tgtcagtaaa
     6001 ataactggtc accgcgccca tgattaatag caaaatcaac acaggcttta gcacatagcg
     6061 atacgatgat aacagcgtgg tgatcagtag catcgcgcca aagagcacga cagcgatcgt
     6121 cagcacaaag ccgagattgt ccgcgatggg ataggtttgg ctgattttat caaaaaaggt
     6181 aagattggcg gtcgcggtca agaaaacggc aacactcgcc acaagaacaa acggactgac
     6241 cgagcgtcgg taccacacag aagtatgctg catcatgaga aactactcaa aaaataaacg
     6301 gtgggataat tgcggcaatt atactgcaat tttttaagaa atccagtaca atttatctga
     6361 tataaaaaat tatgcgatac agaaatattt cagaaatttt attaggcaat cattgcgcaa
     6421 tcccatactg aatatacact tgagagtatt tataatttgt aaactataac caatgccttg
     6481 ttgaataacg cctaaggagt ctaaaaccga aagtttatcg aaagttatac cttcagtatc
     6541 tccattctca tcggtaaata aaattggagt gagtttgttt gcctgccaaa caaactttcc
     6601 aaaataagta ataaggttcg cttcttcttt gcttatagtg gataatagtt ttaaggttct
     6661 acgactaaac gaaccttttg actttgcttc ttcggataaa attcttcccc aaagcatttg
     6721 catttgttcg tcactaatat cctcacactc cctgaagaaa gcctctatcc aatcttcgtc
     6781 aatatcagaa acattatcac tctcagatag actttgtgct gcttgcattg ttatattttc
     6841 aatgttttcc tgtcgtttcg tttcacgttt gagaaaacgc tcaactgccc gtttttctat
     6901 tccctcaagc tctaacctag aaattagctc tgtgcgttta gcttcagctt cagccttcgc
     6961 ttttttcctg attcttgtag gttcataaag cacaccaata gcatcactta ttttttcaat
     7021 aagttttgtt cctggttctg ataatcctga taaacttaaa tcgataaggc tcatctagtg
     7081 ctcctaaata cccgtgcaac aactgtacaa gggtatttta ttgcaagagt aactaaaaat
     7141 gaatcacaac tacatttaaa agtgatgaag ttagtacatt cagataagta tctaaaagaa
     7201 aatgataaag agtttattgg gccacttgct atgattaatc atttctgtca gctctgaacc
     7261 acaaattaat gagttcgttt tcgtccagat cctgataagc cagtaatgct gctttgatta
     7321 tttttgtctt gggacgatgc agtctttttg attcgttatc aatgaattca agcaattcat
     7381 tcggtatgcg gaatgaagcc agttttgttt tgttctctgt ggggcgtttt cctgcgtcct
     7441 gaacaaattt tgtggcgttc acttcctggt tttgttctag ttctggtagt tccagctttg
     7501 ccatgatgta ccttctaatc tgattgtttc acactaatgt atatcatgat gatgtacatt
     7561 agtgtatgtc actatgattt acagttccag ttctttcagc aagagttcga tttgtgcttt
     7621 tgcctggctg gcaccgctaa cattactttt taactcgtga acccccattc cctgattcat
     7681 tgctcttaca aaggcccgga gaaaagaaac cgtgcttttc attggtttaa gccagtaatc
     7741 atggctgttg agagagtctc ttaattcttg ttgttcactg aacgtatttg gctggcattt
     7801 atacaggagc acatgtgcag ccagcctggg atttattttt tgcgcatccc tgacaatttt
     7861 tgtcacagtc tgaagactgt ctgcttccac ctgggcaagc gggtcgatcg ggacaatcac
     7921 aatgtctgca acttttagtg cagttctgaa ctccgtgctg tcatatccgg cagtatctat
     7981 aatgaggatt tctgctacat ctgccatttt tgttattttg gcactgatgt cgccataggc
     8041 ttcatttaat gcgacgggcg gtaatccggc ctgttctctg cgtttattcc attttttggt
     8101 cgagccattc aggtctgttt caacaagccc cgttctcttt ccctgttgtg agaggcaaac
     8161 agttaagttg gttgccagat gtgatttacc tgtaccgcct ttatcaccac cgattacgat
     8221 aatcatagta ccccttgtat gtcacagtga tttactcatg tgtatttcta tgtgatgtac
     8281 gcatgcgtat gtcacatgga catactatag tgtatgtcac aatgaattac aatgatgtac
     8341 atcatgaaga aatacattat tgtatgtcag tgtgatgtac ccgttggttg gtcacttgaa
     8401 agcaaaagtg acagcgttac tttcggtatc ctcccttgct gcgaaacctt gtggcaatac
     8461 agcaatgagt atgtttagtt acaacattca aaattaagcg attagattcc gcccgtacgg
     8521 gcggggcgcg ccactttctg tagtggggag gggcagggag gttatgtttg ctggatgagc
     8581 ggaggatgga atgagctatt agaatgttgt aactaaaggg tatcccaatt aaaggccact
     8641 tacgcggcct ttgtctgtcg gttcttcggt ctggtgacat accacgataa acatcccaca
     8701 gcaacactca taagcattcc ggataagcga tcatcgatgc caggaaacag gcaaccgaca
     8761 aacatcgcaa cagggaaagg aagaagtaaa cagcgcccca aaatgctcat aaaatccata
     8821 aatcctcctt ttttgttaaa gggcaggaag cggttcactt cctgccctta gttacgtttc
     8881 cgttgtcatc aatcgatgaa ttggcggatc agttccagaa ctgcaatcag cgcgtttgca
     8941 acggcgacca cagctctgag aacgatgatt aacatattaa tcatccgttt ctccagtaaa
     9001 actggaggaa atcaccgttg cttgctcata cactgcctgt cacaacggct ctctcgcatt
     9061 gttaggtgta gagtgacctc ctcctgccag agcaccgaat acagcaccac ctgtttatcc
     9121 agccaggact ttaaaatcgt tcatacccgc agatacggga acagcaggat tgcaagccaa
     9181 ctacatgact aactgactga ttatacgcta cacaaaacac ccgttacacc actaaatgtg
     9241 gttccttaac cacatttagt tgctttacag caccatagtg aataaaatgt aaacgttagg
     9301 tgtagagtaa ccttctccgg tcagagccac caactcaaga ctggaaaacg caaaaaagcc
     9361 cgattaccag tcgggttttt ttgtgcctgt cgttcggctg tagttccgtt ttttctcctt
     9421 catttgaggg atttttcttt catttttttt gttgcttcca atactgcctg tcgcgttggc
     9481 caacagcttg acgcgttacc ggcattttca ttactcaata tttttcagtg aattaaaatg
     9541 caacagcgac agaaaaggac attcagcttt ttagatgaat gctatagcaa cggtgtgcta
     9601 cgtaaaatct gcgaaaaaag caggggtagg gtaggttatt tttcacgtag caacgccatc
     9661 tggcactgta cataggtatg tcacacagac atacctatgt gtatgtctgt gtgaggcact
     9721 gttgcaaagt tagcgatgag gcagcctttt gtcttattca aaggccttac atttcaaaaa
     9781 ctctgcttac caggcgcatt tcgcccaggg gatcaccata ataaaatgct gaggcctggc
     9841 ctttgcgtag tgcacgcatc acctcaatac ctttgatggt ggcgtaagcc gtcttcatgg
     9901 atttaaatcc cagcgtggcg ccgattatcc gtttcagttt gccatgatcg cattcaatca
     9961 cgttgttccg gtacttaatc tgtcggtgtt caacgtcaga cgggcaccgg ccttcgcgtt
    10021 tgagcagagc aagcgcgcga ccataggcgg gcgctttatc cgtgttgatg aatcgcggga
    10081 tctgccactt cttcacgttg ttgaggattt tacccagaaa ccggtatgca gctttgctgt
    10141 tacgacggga ggagagataa aaatcgacag tgcggccccg gctgtcgacg gcccggtaca
    10201 gatacgccca gcggccattg accttcacgt aggtttcatc catgtgccac gggcaaagat
    10261 cggaagggtt acgccagtac cagcgcagcc gtttttccat ttcaggcgca taacgctgaa
    10321 cccagcggta aatcgtggag tgatcgacat tcactccgcg ttcagccagc atctcctgca
    10381 gctcacggta actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac
    10441 gctgaaaatg ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat
    10501 aagtttatca ccaccgacta tttgcaacag tgccctgtgt gatgtatact tggcgggtta
    10561 ttctgcctgt tccggcttag ttttctcaat gaggaaatct tccatgaaat gcccctcatc
    10621 aagcagcttc tgtaaagcaa gcggacgctt gccattacca gaccattctc tgatctcgcc
    10681 attttcatcc ttaaagcggt atttagcctt tcttactccg cctttggctt tccgcctggt
    10741 ttttactgta tctgctgtta caggagcaag gagtgattcg ggatcaagac ctaaagatat
    10801 gatgtattca atggcctgct gacgtttggc ttcaagttcc tggcgttcag ccgcttcacg
    10861 ttcagcatct tcacgacgtt cttcaataac aacgttgagc ttttcctgca tttcgagcaa
    10921 ctgctcaaaa tcaatctcac gggcaaaaac acgcaatgaa cgaatattca tcatagtacg
    10981 gctgataatg ccgtattcat cttctttagt cagttcgctc atataattaa tcctcttatt
    11041 tagtgtacgt acttccagac gccggaaggc accttatcat aaagtttatt catcaccagt
    11101 tctgctcggc gatgatctgc ggcagaataa aatgcctctg attctgataa aggcagtttg
    11161 taacggttaa tctcaataac tttttcaaga gtatctcttg atgtacattt tcttaactga
    11221 aaaagccatt cctgctttgt tttcacttct tagcccttgc cggaaaaccc agcttccctg
    11281 ttttcttatc ctcaaggaca agaactgtat catacgttcc accacttttc tttttaaatc
    11341 ccttaatcaa atcggttttc cctgatttaa ccagtttttc ggcctgtgcc tgggtgattt
    11401 tcttaccaga aaactcactc cagattttaa attcacatcc ggtacagaaa taacctttcg
    11461 gcctgataac aatatgttta ccacaggaag ggcagggaga gtccaggcgt tgaaactgcc
    11521 ctgctggcgc agcgggttca atcttcatct cgcccaggtc aacatcagaa atcatgcctg
    11581 tcagttcacc gtacagatca ttaataaact gttcaaccgt caggtcgcca ttttctatgg
    11641 cagcctgctt ttcagaccat aatgctgtca tatcaggatt aaccgctata ccaggcaggg
    11701 catcaataag cgcatatccg gtatcagtag gaataagttt ccctttttcc agcgtgatat
    11761 agtttctttt cttcagcgtt tccagaatgg ctgcgcgggt agctggcgta ccaatgccac
    11821 catgttcatc ttttttgtct ttatccttat ccttcaacaa ttttttaatt gttggatcag
    11881 tgacaaaatc cgcgacacga acaagcgcag caagcaagga ggcttcggtg aataacggcg
    11941 gcggtgttgt tttcttctca ttaacaataa cttcttttgt cgtcagtgtt tctcctgtgc
    12001 gaattttaca gagcagttca aaagcggaat catcattatc ttctgattca ccttcgtctg
    12061 tggtttccgc gccaagaaac gcctcaaatc cgctgtcagt tgtttttctg gcacgggcat
    12121 agaacgactc atcaccacac tgaatggcaa ccgatacttc ctggtatgct ttttcaggca
    12181 tgaactgaac aagatagtgt tgtgcgatcg ccaggtaaac attgcgctcg tcggtgctga
    12241 gtgcgttaac atcaggcaca ctggcggtcg ggattatcgc agtatgcgca gtcacctttg
    12301 cactgttaaa cgctttgctt ttacgtgcag aatcaatatc cagcgactga ggaaagactg
    12361 atttcagggc atcgataacc tgcggcgctt cgctgaattg ttcatctgaa agatatgagc
    12421 agtcagagcg gttatacgta attgctttat atttttcgcg tagttgttgc gtaatatcca
    12481 gcgttttttg tgccgtcatt ttaaacttct tgttcatgta ttgctggagt ctgaccagat
    12541 taaacggcaa cggcgcagcc gtttttttat catcagttgc tgctgcttca actgtagccg
    12601 gttttcctgc aagggatgca gccgttccgt ctgcccacgc tttatcaagt aatttacggt
    12661 ctgtcagcgg agcaaattcc cctggcttcc agttcgccct gataacatca gcaccacgct
    12721 gaaagactcc ggtcatggtg tagtaaaaac tggatttatg gttctggtta gcacgggtac
    12781 gattcacaat caggccaaga acgggagtct ggacgcgccc gacagacaga acgccctgat
    12841 atcctctggc ttttgccgga atggtgtacg cacgtgtcat agagaagcca tagacggcat
    12901 cggcaactga ccgcgccaac gccttaaggt aaagtccttt gaaatcacga ttgtctttaa
    12961 gatttgccag tgcctttttc actgccggaa gcgtgttgtc gttaatcaga acgcgcttta
    13021 cgggttttgt gttgcctgca tattccagga cttcatcaac cagtagctgt ccctcatcat
    13081 caggatcgcc agcgtgaaca atttcagtca cgtctccacg tctgataagt tcgagaatcg
    13141 ttttaacctg ttttgctgca ctttcaacag gctgatactt cacgggataa agacgtaaag
    13201 gaagcgtttc aaccttccag gctttgtatt caggattata gttttccggc ggttgtgatt
    13261 cgataatatg accaaaacag ttagtcacaa tggcgttatc actttcaaac cagccatcat
    13321 ggcgggtaaa attgccacca agtgccttaa caatatcatt tgctactgcg ggtttttctg
    13381 cgataaaaag tctcattttg ttatccttta cattgaatta atcttttggc acaacggata
    13441 gcgcgttctt ccgatactgt atgttgccag ttatctttac ttactactct gaatctgcgc
    13501 tcacagccat taaaggcaaa aattccctca cggttaagta tattagttgc aatatcccac
    13561 tctttaattc tcaaaaaggc tgttacagca aaaccagaat acgaagcaat ttttgaaatg
    13621 atgcattcat cagcttttat taacgcggca aaaggaacta tcttttgatg accacacgaa
    13681 atcagaggga gtgtgtgaat atcataataa gctattgaat gacaatccat gcgggtatca
    13741 tcaatagctg ccagccagaa tcctgcctgg tcttttattt ttctgctaat atctgaatct
    13801 gaaacaccag ggtttaataa atatccacca taacagatat ggaaggggtg agcagattca
    13861 tcaatatcag gaagttcttt taccggattt tctttccagt ccttcagaac accgaattca
    13921 agagcatcca gcaaaaactt tcgttcaaat ccatcaaaca atgcatattg catgtgttaa
    13981 tcctccaggt aacttaaata gtcaggttgt gccccgttct ttttataaag ttcttccctt
    14041 tcctctatat cttcatctgc ttcctgaacg ctcagatcga gggttccttt caaaaaggca
    14101 cgagcatttt ttattacatc cacttatgca ctaccagcag ataagcacaa taaaatagct
    14161 ataatagatt tggttacttt catttctaac ctcagtgatt gtaactaatc atcacatttc
    14221 gttggcgcga aatgtgatac ttgcagtacc ctgatacccg ttggcgcggt tatcaggttt
    14281 taattaacct ctgacacgac caaacttact catgattttt gatatagcag caggatcagc
    14341 cgttgaacac tgattcagaa atgctttacg ggcatcaaaa gttttactcc agcgaatgct
    14401 tccttttttc tttttcagaa tattaaaaaa atccttttca gcacttctgc attcactccc
    14461 tccaccttgc ccgactgctt ttccatataa gcaaagtacc gaagcgcaag ggtcagatgc
    14521 attagcattt gttgatatta atcctaacgc cagcaaggct ggtaaaacca ctctattttt
    14581 tccagtcatt tttcacctca ttttgtgctt taatgaaaac tatttttacc tgttcaggta
    14641 aaaaactttt ctcgtttcgc cattccacga ctgctgttgc cccttccatc ttcaacagat
    14701 ctctgattcg aaaccacagc ccagcatcat gtgttgtcac tacaatcctg tccgcatgag
    14761 ccagagcgta ataaaacgtc ggcgttctgg cctgtactcc ggataaggaa gtgatctcat
    14821 aaacccatcc tttaccgcta acgtctgact ttatgacacc gtgaaaatcg gggacatatg
    14881 gcaatgaggg attgatcact tcatttgcct tttcaaactg cacgggtgtt ggctctggcg
    14941 gtgatgaaca accagcaaga atggaaatca ataaaaacac taacttcttc atcatgttct
    15001 cctttatgcc gctttatcga gatcaggaac atcttttact accagatacc cttttgcgac
    15061 gatttcattt tcaaatactt tctgtggagg cataaccagc tttcctttct tcctggggaa
    15121 aatgtttgca agttccggag caaccttcac aaagtcacca aagaaatact cattgagata
    15181 gtagaatgcc ttttcacaac gaatcgggtt ttctcccttc attgccagga tttcttcact
    15241 aaatttcagt gtaagtagtt catttgttgt taccagtgga cgctcaataa ggctttctga
    15301 ttcagatctc gatgttcctc cacgactacg acttttgctt ttcgaaccga tagttactgt
    15361 tcgtgtcccc atcgcttctg ataactcccg cgcatcttta acatcagaag cggaataaac
    15421 aattttgcag ggtgcacttg ccagtaatgt tttactgcct gcataactgt agatttcctc
    15481 aagctgagag ccgacctggt aaacaatcaa caatttcagc ttgtaaccag caatgagacc
    15541 agatttactc ttgatgtaat ctacagcacc aattgacggg aattcatcca gtagaaaaag
    15601 gcagtcatgc tttatttccg gatcatccgt tggcatcttt tcaagattca cctgtatagc
    15661 aagctgaaag aaaatatttg ttattttttc agccaacctg gcattatcac cagaaataca
    15721 gaaataaatt gtcatccgct ttttccgcac ctgccgcaaa tcaaaatcat ttccatcagt
    15781 agcatttgcc acattggggt tagcaaacgg tgataatcca ttaagaaagt tgcttttgag
    15841 ttgtgctttt gttttatcct ccatctcaat gaatgcctta aggccagcga caccaatatc
    15901 agcaagcgtt ttgtctttcc cccttaattc tgaacgaata tcattcatga atgaaagaag
    15961 atgttcgggt tctgcacgct caaaaagctg attaatttta gacattgaga agacaggctc
    16021 aatatcaagc gtttttagtc cagccttatc attcttaatc ataaaccata aagcgacagc
    16081 ataagattta aaaacaccac ccgccgtctt attaagatag ctaccagcat ccgcaccatc
    16141 tgtcttatag gggtaaataa tcaacgaaag attttcaagc tgattaaaac cttcactcgt
    16201 tcccatgctg acataataca aagggttaaa gcggtgcgtt accgaactaa agggatcaaa
    16261 tttataaacc tcatgaccta aaatcacctt cctgataaga ctggtgatat taaaacactc
    16321 cctttttgga tcgagaataa taacactcca ccagtacttc ataagattag tgatgactat
    16381 accagcacct ttaccagccc ttgttccggc tcccatagca ctgaatgctg ctcccacata
    16441 gcgtatgatt ttcttgtctt ttataccaac gataatcccg tctttttcgt caccgtcaag
    16501 cgtgaaactg ttagactcac gaatatcatt aattgttgca aatcgcgcat caccgaaaat
    16561 attttctttc ttctcgttca gttttatcaa cgccgcaaca gggatgataa gagacacgac
    16621 aaatcctgct ccaagtgcaa caaaggctaa aactctgata tccgtagtca caacagataa
    16681 agtaaggctg tctgtaaaaa tatccatcct gtacgcatca atggatctga acacagcctt
    16741 caatggtttc agattcttta tctggctgaa gtaaagcaca aagaaaataa atgaacctgc
    16801 aaaccatgcc gcaacaagca taacagcgaa tataaaaaca gattgcgact tattaaaagt
    16861 tttcacattc agcccctttg taatagatgt cgctcatatg cctgacaaca ccatccctgc
    16921 ctacatgaac aacaatatca atactgtcca taatgatttt acgaagaaca tcaaaaggaa
    16981 gattctgaca ttctctgttc tgataacatt ttgtcaccat ctgaatgatg gcatcttttg
    17041 ccgaggccgc gtgaatggac gtcatgcttc cactgtgacc agaaccagca actttcacaa
    17101 aatcccaggc atcgccacct ttaatttccg ccagtaaaat tctgtcaggt ttcatcctca
    17161 aacaggattt gattaatttc gctgctgtta tgatagagcc ggaatcactg ccggattccg
    17221 aagggtaaaa aagatgcaca taatttttat ggataaaaaa tttgatttcc tctgcatctt
    17281 caatcgtaat cagccgtgta ttcaatggta tgaaatcgat aagactcttc atataagttg
    17341 ttttaccgga acccgttgct ccggcaaata caatggtttt ccctgctgct acggcaagct
    17401 ccataaaagc agcgatattt ctcgctctga ataaatcaag aagctcatca tcgtatgtcc
    17461 tgtgtttttt tcctgactgg acataatcat aaaaaccgtt cgccacatat tctttatgtg
    17521 ttatctgccg tgtcgatggt ttacgtatag tgattgaaat agtatttctc tcacatgcag
    17581 gaggaaaaac gacctgtaca cgttctcctg attccagtgt tgctgaaagt aatggcttgg
    17641 tctcgccgat ttcatctccc cgaaatgatg ccagcgtatg agaaaaacgt ttacaaaaat
    17701 caaaggtcac actgtcatta ttatggaatg tccagtcacc attaatttcc gtccagattt
    17761 caccaggacg gttgacagca atttctgtta gcccttcaat atcaagaaaa gactggaata
    17821 cttgattttt ataaaaatcg agcgatttat tgctaatgct gacaggaata acgccactca
    17881 tactatttta cctttaactc atagatatca gaaaaatcaa tgtcttcccc gaccataata
    17941 gtgattatgt caccctggtt tttatacatt gttggaggaa tatttatcga attctcaagt
    18001 gcgactttag ccatttcagc cattgcctgt cgtgagtttt cggtataatc cacattacga
    18061 tctttaccag gtgcattatc cgccgccgcc gccgctacat cctgaaccat actgagcata
    18121 agagaaccac caaaacgctc ccagaagtga gaatcgatcc atccatcaat gcccgcttct
    18181 cccaggggac cagctgctgc tgtatttacc atatcgattc gtttattatc aggcgtccgc
    18241 aactgctccc acataacgaa cattctgccc tgtccatgat tcagcgttcc ggtctggtaa
    18301 cgaccaaaag ccttagttcc ctgttccagt aattttgtgt ggtgattagc actccataca
    18361 tcttctgtaa aaacacaact gatacgacct gcaacatcag aaacaaagcg tgtggttaaa
    18421 gcacacggta taagcctgtt ttcctcaata tagagatcag gatcgagatt aatcgcagtt
    18481 actttcattc ctgatttacc aggcacattt tcttttgatt ctttcctttc ttcctgtggc
    18541 tctgatgtct gcgatgtcct gctgctgcca ctcgcgccag tctgactccc tccctgacca
    18601 gcaacaggta ttgacaaata tttcctgaac ccctcctgtg gcacttcttt atgaccgtca
    18661 gaaacagtat taccagaacc agtagcgcct ggattgataa ttgctttgtt ctcaacaggg
    18721 tcaaaattct gaccaagacc agtacggatt ttacttacct gagaaatgct ggtatcgcct
    18781 gttgattctg atgctttctg ctggtcatct gcaaaataat ttttatatgt tttcagcgca
    18841 aaaataccaa taactaatac agccatcaga ccaaacagaa agccagaacg catacgatta
    18901 tttttgttcc tgatatcacc gatatcatga acgggtttac tttccagctt tgactgattc
    18961 gcatcatcaa ctaaatcatc tgtttttttc atatcagtca ttattcacct caacacgttc
    19021 taccgcagga gaagatgtat tcctctggtc ggttaatact tttccgaaag atttattcac
    19081 aacaccaaca acgctgtttc catagcgtaa aacaaatttg tcattaacgt tgtggataac
    19141 cataatttta tacttgcctt ttgtttccac attaaaagca agtgtctgtt cctgaccatc
    19201 tcttacagca aatggagctg ggaatacttt ccctgagtta aaacctaaaa aggtaaatat
    19261 gccattatca taagcaaaat caggggcgat acgacgactg tcatattttt cattgactcg
    19321 catgaaataa ttccagtttt taggcgcatc ggctttttca aaactttcag aaatcagttt
    19381 cttctgtctg agtttttcct gtttttcttt tgccagcctt acttcttctg cattcttttt
    19441 cgctatttct gatggatagc ggaattgtac aacataagca ggttgcgctt gtttttcttc
    19501 tgacagaagg attagatcca gactgtaaat ccgctttgtt gttctgacaa aaagattcgt
    19561 atcccattcc ttttctgttg gctggaaaac ctttttcagt ttttcgcctt catcgccttc
    19621 ctgttcctga acaacgggac gcggattgat gtaaacaaca ttatcatcgg ttgtgatttc
    19681 ccatccggca ggaaaaccag cctttgcact gataacagcc tcaccttcat caaaaacaac
    19741 tgatgtgaga aatcctgtct ttgaccggat aacggtcgta ttcgcaccgt tataactcac
    19801 agtctgcatc cttgcatcaa atccagaagg taacggagtc gctgctgaat acacaagccc
    19861 aggaagcaat aaacatgtag caataaataa tttcttcatc attcccccct gacttcccga
    19921 tctgtctggt aactggttac agtaaaaccg agaggattaa gctcacgact ggaactttta
    19981 accgactttt ctggttcaaa acgatatgtc acacgagccg tccagtatga aactgaaacc
    20041 tgtccggtcg tgaagtttct cgtcgttttt ttaaagcgca cagaggccag tttatctggc
    20101 gcggttgcgt cagaaataat attggagatg acctcaaccg caacgtaaga accgttatta
    20161 tgataaattt tatcaggagc catatcgctg ttatacagac gaagataatc gtcctttact
    20221 gtattcgagc tatatagctg tacagtctca taatcatact gaagagacgg ataattataa
    20281 ccttcacgta aagtaatata cgtgtttaca aaataccgcc caagtgcttc agattgcagg
    20341 atcttttcct gtttaacctg agtaatcagt tctgtacggc ctgttttttc gccaacagcc
    20401 acaatagcaa gctctttttc ttttaatggc acaagaacac aaattgcagc cccaagacat
    20461 aaggacagta atgatgctgc cactgcaaca cgccatgcat ttttcttaga ccgttcattt
    20521 tctgcataga gttgtctttc gaaggattta gctgtctcaa tcttcttttc gattttttca
    20581 ctcataactg attacctggc tgtggttaat gggttccagt tcgccggaaa catcagaagg
    20641 aaggttattc cttgtggtac atccggaaat ctgaaacagg aaaaacaaaa gaatcacaca
    20701 tcgcataaat tcaccttatt aagcgttcac gaacaccttt aagagataaa ggccaagatg
    20761 aagaaaaaga atcccaccaa taagaagcaa aaagcgaaga tccataccct ttctccattt
    20821 ccccgcaagc cctaaaccaa gtcttattcc tgcgaagcag gtaaacactc ctaaaagaag
    20881 gattaggaca aaataaagta tcatgacaaa ataatgaagc atgtcacaac cagctctgac
    20941 tgattccacc taagacctta aataaggtaa ttcccgtgat taaaataaca agctgggtta
    21001 ttagctttct cttcctgctc catccggagg tgatatgttt atcaacgaac accagtatcc
    21061 agttcaggga catcacataa aaaaaacgtt tcattatttt catgcggcgt tcctcgctcg
    21121 tgctttttcc agggatgcaa gtcttctcgc cttcatcccc aaagcacctg tcggagccag
    21181 cgcctgatat cccttttcac ccgcccattc agcggctgtt cgcgcaccac gccccatcag
    21241 attccctgct ttccctgata aagaattcat gcccgttctt ccggccatcc ctaagccaaa
    21301 acctaaacca gcacgaccgc caagcatcag agacttacca gtagccatac cagcagcacc
    21361 cagccccatc atggctgcgc cctgaacagc accttcaaca cctgcacctg ctaactggac
    21421 agcaaaacct ttagctataa gaaccaataa agcccccaaa aacccagcca taaagcccat
    21481 agcacctgta gatacaagat ttacattccc tgtttgtgca gcacgaatag catgggaaag
    21541 gatcatccct tgaaaatcca ttgctatccg aatcactaaa ctggcaaata gaactgtcag
    21601 aatactggag aataacgatt gtaaccaatt attaaacatg ctccgaataa aaccaaacat
    21661 gagacaaaag ataaatatcg gtgctgtaat aatcaggagt ttcatcgtta catctgctgt
    21721 taaaaaaaca accacactaa cagccattaa aataagcgag ccagtccata ttaaaaactg
    21781 accaactaca ccttgatctt taacatatgt tgatttatct aaagagtata tttccgcacc
    21841 aagattttgc gtacttttcc ataagttatc taaagtttgc cagacactca cccctccaga
    21901 aaaaccatct ttcatgcctt gcaacgcatc agtagccgca gttaaatacc catccgcatt
    21961 agttataaac atgattatga ttgcaaattt tgatagatcc catactaaat cctgcaaggg
    22021 cgtctgagtt ttactggcaa gtatttgata tcctttccat agtacatata acgtaaccga
    22081 taccgttgct aacgacataa tatcattagc aaattgtgtt tgataactgg agctgatttt
    22141 atctaccgaa tccataacgg tagaattata tttaacgaaa aacccctgag ccatatatcc
    22201 accttttaat caaaagttaa tggttcagct tctttctgct gtttaataaa ggcctcttgt
    22261 tcttgtgtga ccaacatctc atgatctgct ttatttttat tattccagac atcatactag
    22321 actggccccc tgaatctcca gacaaccaat atcacttaaa taagtgatag tcttaatact
    22381 agtttttaga ctagtcattg gagagcagat gattgatgtc ttaggaccgg agaaacgcag
    22441 acggcgtact acacaggaaa agatcgctat tgttcagcag agttttgaac cgggaatgac
    22501 ggtctccctt gttgcccggc aacacggtgt ggcagccagc cagttatttc tctggcgcaa
    22561 gcaataccag gagggaagtc ttactgctgt ggccgcagga gagcaggtcg ttcctgcctc
    22621 tgaacttgct gctgccatga agcagattaa agaactccag cgcctgctcg gcaaaaaaac
    22681 gatggaaaat gaactcctta aagaagccgt tgaatacggg cgtgcaaaaa agtggatagc
    22741 gcacgcgccc ttattgcccg gggatgggga gtaagcttag tcagccgttg tctccgggtg
    22801 tcgcgtgcgc agttgcacgt aattctcaga cgaaccgatg actggaaaga tggtcgccgc
    22861 agccgtcact cagatgatac ggatgtgctt ctccgtatac accatgttat cggagagctg
    22921 cccacgtatg gttatcgtcg ggtatgggcg ctgcttcgca gacaggccga acttgatggt
    22981 atgcctgcga tcaatgccaa acgtgtttac cggatcatgc gccagaatgc gctgttgctt
    23041 gagcgaaaaa ccgctgtacc gccatcgaaa cgggcacata ctggcaaagt ggccgtgaaa
    23101 gaaagcaatc aacgatggtg ctctgacggg ttcgagttcc gctgtgataa cggagaaaaa
    23161 ctgcgagtca cgttcgcgct ggactgctgt gaccgtgagg cactgcactg ggcagtcact
    23221 acgggcggct tcgacagtga aacagtacag gacgtcatgc tgggagcggt ggaacgccgc
    23281 ttcggcaacg agcttccggc gtctccagta gagtggctga cggataatgg ttcatgctac
    23341 cgggctaatg aaacacggca gtttgcccgg atgttggggc ttgaaccgaa gaacacggcg
    23401 gtgcggagtc cggagagtaa cggcatagca gagagcttcg tgaaaacgat aaagcgtgac
    23461 tacatcagta tcatgcctaa accagacggg ttaacggcag caaagaacct tgcagaggcg
    23521 ttcgagcatt ataacgaatg gcatccgcat agtgcactgg gttatcgctc gccacgggaa
    23581 tatctgcggc agcaggccag taatgggtta agtgataaca ggtgtctgga aatatagggg
    23641 caaatccaca tactgcattt ttatcgcctg catttttaac gactgcgcct gtaatgcgtt
    23701 agccaaatcc tgggattctt tgatatcctt tgaagcctca attcggctac ttaaactctg
    23761 aatctgagac attgcacttg ataactgctt attgatctca tttccctgtt caatactggc
    23821 agccgttgac acaatccgtg ctttacagag attatcatta cgttcatagc ccgtattaca
    23881 catatcgaag gctccatact tgctgaacag atttttagcc tggctgctca atgcaccttc
    23941 gggattactg gtaaaatcag aaatataact gttcccctgc ttataaatat ttgtcagttc
    24001 tgattgtaga ttacttagtt catttgtgaa tgccgcaata ttccttatac ctgttgccgt
    24061 ggctaactgg tctttatatg cctgaatctg actctgataa tgttgcgcag tttcggacca
    24121 ttgtttgagt cgttctgtcc atcttgcggc ttcaacagcc cattccggac tggcatcaat
    24181 agcaaccgga attcctgcgc tactataaaa tgaagccgtc aataatgata tcagcatcaa
    24241 tgtttttaca cgtttcataa attcacctgt cagattgccc gttcgagata ttcaggaacc
    24301 cagtcatcag gctccattcc ttcatgataa atactttcaa aaatttccag attatctgca
    24361 ctggcagaca atattttcag atatccacca agtcccgata aatcgagtgt cgcaagtgct
    24421 gaaaatgatt taccatcccc ctttttaaga ctgctttttt taatcaggaa ctgtctcgac
    24481 attggatcaa gatttttaac aatattaaat tcttctggtg tcagcttaag cccttccaca
    24541 taatcattgt aatcggcatc agggttagcc agataaatac tggtgctgca tacttcaacc
    24601 acagcccgtg aaatcttatt cttcagtatt tcatccggag actgagttgc cggaataacc
    24661 agcccgttca gcttacgaat tgttttcagc ttgttataga caaagtcgct gaatgcttca
    24721 tcctgtagcc atttccagaa ctcatccagg aagataacaa gacgccgacc gtccagaagc
    24781 tgcgtaatgc gatacagcag atagaaagta attggtgcac ataccatcgg atcatcaaga
    24841 aactcggtgc catcaatgcc aaaattattg acatgctgaa tattgaatgt gtctttagca
    24901 ttatcaaaaa cccagccatg agcctgacca ttagcccatt gggacaaacg aaggataatc
    24961 ccgttctcct gctcatcccg atcatctctc tgcataagat gctcaagcat tctggtaatg
    25021 ccatattccc gcatttcgcc aggaggaaaa tccatcacag catcaacact ttcgctaata
    25081 agcaatcttt ctcttgtcga taatctttca ccatttctgg tacacagaat ctccatcagt
    25141 tgcttgacaa aaatacggtt acgttttgtt gcctccagag caaaaggatt ccatcctgtc
    25201 ggctcaccgc tttttacggt gtaatattcc ccaccaagca ttcggataca taattcagcc
    25261 cctctgtcct tatcaagaaa gacacacgtt aacttcttat tttttgcaga atcagcaaaa
    25321 gtttcggggt tattgtattt ctgtaatgaa caggcaagat atgacagaaa cattgtctta
    25381 cctgaacccg ccgttcctat aactttagta ttagcaagat tcttttcacc tgtttcatct
    25441 ttaaacagta ctgagttatg taaattcaga taatacgcct gtttgcttgg tgttttgaga
    25501 atagcaaccg cttctgtcca gcagttttta tcgcgtttcc cctggtagaa attatggaat
    25561 gatgccagtt caacaaaatt tacattcgat accggagata aacgcggcct taaatgaaaa
    25621 accgccggaa gttgggcaaa atatgccgct ggcaatgaca tggttgacaa tgccggaata
    25681 atgcccaggt cagtaaagtc tgccgtaatt ttacttacag attcgtcaag ctccctgata
    25741 ctgtccgcca ttaccattag cgtgaaatgg tagtaaccaa aggatatatc accggacaca
    25801 atatcatttt ttgccttttc cagttcctgc aattgagtaa ccgcatcatc ttctgtactc
    25861 tttaactgtt tttccgcacg tttaatggta ctcaatgctt ctgatttgct catggatgta
    25921 tatgaatgcg taataatata gtcagcatca gaatactgga gcacatcaaa aacaccggat
    25981 gcagtctcac tacagaaatc tttaatttca atagaacgaa agaatttatt tccatcaatg
    26041 cggcatatct gtccggtatc atgattaaaa aatacatcca ctccaccaag aacgttatac
    26101 accggagaat cagtgactct gattttttgc aacttccctg tcagaagata attatagaat
    26161 gacagttgag aagaaaatac ctttccgttc tcctcataca taccaagaag tttacaggta
    26221 aatttatcaa gagcgccaga gaatgtatta atcatttcat tcattctctt cacatgaatg
    26281 ttaatatcat ctttcttttc tttaatactt tttttgcgtt tttccagttt ttctacacgc
    26341 ccatctggtc tgtagatcag cgtaaagtaa agagtgttcc ccttgaattt atttttcttc
    26401 atcgaaccat agtaacaatc tgatattata tcagcatatt tattacctga ttttgtggta
    26461 aaggcatcat aaaaactcgc cctgcatgaa tggttataaa atgaaacggg cagtccggaa
    26521 aatgaacgaa caagagtggc aacctgtgag tcaatagttt ctaacatctc cgcagagatt
    26581 gactcaaagt taatccccat cagttgccat gtcgctacat aatcaccgtt ctttgttttg
    26641 acaatatttt tatcaacatg gcttgaatag ggtatatatt taccagtagt gattcgctca
    26701 ttcagtttca tggcattaac aaactcatca atttcaacag cttcatattg tccggagagg
    26761 aacgctcgtg cgccaaaaaa acgattacac actgagtttc cgagcatttt cagcttaaga
    26821 aagtaaaggt taaaaatgtg gtcgtcctgt ttagtgatca ttctgagaac aaaaaccgcg
    26881 acaggaatag ccagccagat cagcttgctg atataaaccc ccgccagaaa taacgcccct
    26941 gcaaccacag taagagggac gacaggaaca cccacataca tagcagggcg tgtcatcgct
    27001 ttataaagtg tagacatact atcgtccccg ctttattagt taaccatcat tgatgcaact
    27061 tcagccgcac tggcgataac aattgccccg atgatgatag gtgagcattc acgaatcgtg
    27121 cttccaccaa acagaacctt gtagccgacc cacagacagg caatagtcac cgtgaccagt
    27181 gaaaggccac taagccctgt tttaaccttt tccagcagtg tttccgcttt tgaaaatcca
    27241 tccgcaaagg ccggtgatgc aactgttgcc ccggcgactg ccataactac cttattttta
    27301 cgctgcatta cagcagtgac agatgaactg atacgattac atactgattt cttaaatgac
    27361 attttataag tcctgttaat taataacttt tgttggatgt gagttatcaa caaaagcaga
    27421 tttcagaatg gtttcgggcc agataactga cgcattgtgt gaacgatttt ttcgctgttc
    27481 tcccccgttg cttcttgtac ctggtacaac atattttttt cgttccctgt ataaccaatt
    27541 cgctcaacat aacttgtatt attaaattct ttttcttttc gcttaccgtt actaaaattt
    27601 cctgtatagt aacaactaag cgcattctcc aggctcctcc ctcgtttata acaatccacg
    27661 ataatctttt cgaaaacaga caagttttca cacgaatcaa ggagtttttc tgcggacgtg
    27721 gaataaaaag aaaaattact gctggtaatc tgcatcaatc cgacagaata acgtcttttt
    27781 ctgttttcaa tttctctgat aactgttaga gcttcctcct tacttttggg catatgactg
    27841 ataacacttt tgccatagct accgcgttcc ctttcaggaa taatttcagc cacagcataa
    27901 ggattgaagc cactctcgac ccgtgcaata tcatttatcg tatccggatg aactgacgcc
    27961 gcacacagca acaaggctgg gaataaagac gccatagcta acgctccata tatccatcaa
    28021 tacgcatact ctgcattaat aagtaaataa tttgcttcct cttactaagc aaagatgttt
    28081 tcgttttctc ttcgataaat gcattcaacc tgggatcgag ttttatcgtg acataaaccc
    28141 ctttttccga tgacttatct gatttctcct tcatttaatc aatccactat aagtgtgttt
    28201 tggatttgtt ttagatgcga acatgattcg caaaataaca atgctcaaca atacgatcaa
    28261 ataacattga caaaatggtc atattatgat tcgtgtgatc atgcaaaaaa aagcgcccat
    28321 atgggcgctc tagtatgatt gaaacattag ttaatcgaat gtagggactg gagcatcctt
    28381 ctgagactga ataaacaatg cttgtttaac attcttacag ttctgagaat cagagccaga
    28441 cttctcacac tctgcctgct tttgtttagc ttccgtaaga tgctgccccc accaatccac
    28501 actttttact tcttcactac aaccaacaac gaagaacggc aaagcaaaaa gcagatattt
    28561 tttcataaat tccccctctc actgatcctg gaaagacttg acacgagaag ggcattagtc
    28621 cgaatgagta ccagtgttct gatccctgcc aacgagacac ctcccagttc agctcttaca
    28681 gaatcgagat attttctgac agttgaaggc tttagtcctt tgaaatcagc aatttcattc
    28741 agggtatagc cataagcgta catcagaata atgtcttttt gttgctcact caaatgttct
    28801 tcatcactaa aaatttgatg aagtccagaa atgcagatag catcatgctc agtcatgata
    28861 acctccctcg taggttgttg tgatcagcag gtgagcaaag tagccgcttt gttcacctgc
    28921 gccaaaatca aattaaaatc ccaggccttt ccagttcctc tcttgctgta ttcttagttt
    28981 tttatgttct tctgaatcct ttgtaaaccc gtgttcaaga gcaaacccga tattgtgacc
    29041 ttgctcatgt tttctggata gctgcttttt aacgtcgctc tcttccggct gcctggtttc
    29101 acgctcatga acctgacgtt ctctgttccg ctcaagggca atattttcaa tctgccagtt
    29161 attgcgatgc gtttccctgt agcctgcaac agttccatca tcattaaaca ttggactacg
    29221 tacaagcgta ggagccagtt tttttacttt gataagcgtt cctggctgga gcttttcaga
    29281 ctcgaaatga tttttcaggt ctttacccca aatcgtaacc tgttttccac cttttaaagt
    29341 ttcataagtg aggaaaggtg ttcgtttttc ccctgcctta ttctgatatg aagtctcccc
    29401 aaaatccacc accttgtaaa cattccggag tcttttcgga ttttcagcct gtaatcgctc
    29461 aatctcccgt ttacgttcgg gatctttttt ccaggtagcg gtaacatcat atcccatacc
    29521 tttcagtttt ctatgaaaac cgtttctgaa cttcctggtt tcagatttag taagtcttaa
    29581 tcttcttcct gtttcttcat cacgaagttt aatattaaca tgtacatgag gatgatcttc
    29641 tttatcatca tggtatacgg caacgaaagc atggtttgga taagtctctt taagaaattc
    29701 taccgtagct ttcagagcgt cttccctgct tactccagct tctggtggtg gtgaaaagac
    29761 catgtttttc acatgatcga tgttttctcc acgatatgtc ctggagtaat catttccttt
    29821 agataaagtt gaagtaaact ccctattaaa gtcttctcct ttccctgttt gctctgaacc
    29881 ttttccatca taacagtaag cctcaagttc accattacgg gtaatgtaat caataccatt
    29941 tttgatgcct gcggctgttt tacctgagcc agtgatttta aaagtgactt cttttgtagg
    30001 tgtccaggta tatgctttct tgtccgcacc atgtctgact ttatgattaa aagccgattt
    30061 actggcatgt atatgtcttc ccgataactt aggagatttc ggatcacgcc cagtcccttt
    30121 agcgcgttta atacggtatt cctgttctac atacacaccc ataaatcacc caaatctgtt
    30181 aacaacactc ttggttaacg tttcaagttt tgaatcaatt tgcttacaca gttcaatcac
    30241 ttcaataacg tcttttctga aatctggatc gttaaccgta cagaatcttt caccttttat
    30301 gataaaatga atgttgcgac caacacgatc tacagcagtt gatagttttt taagttcctt
    30361 aacttcatca gggtaaagac agacttcact ctttaacaaa gactgacgca cacgaatagc
    30421 aatttctctg ctcatgctca tgccgtgaag agcagcatta cgtttaacaa gttctatttc
    30481 atgagggtaa agacgcactg ataccagctt tgctggttca ttattaactg ggatgttaac
    30541 aggcttggtt tcaatattaa gcacgttatg cttagcaaaa gcattgatac tggtaaatcc
    30601 acttaaaata gcgttatgtt ctgcaaattt taactcatca tcacttagcc tgaacgctat
    30661 catcttcata agtcacccca acagagcctg cggcaggcac aggcaaaaac atcgtttttg
    30721 gacaatctga ttcaaatgtt aacatttgac atcggattgt ctatcctgca ttgattgtac
    30781 catatgtggc actgtgcctg gctataaaat cgtcgctgca tatgtccaga tttcgttgct
    30841 gaaagattct ttcgtcttcg accatcaggc aaacgtatag ctgaaagatt tgtgaaagaa
    30901 aaaacaggcc tggcagggct gggaattgtt acggctgaag ccgtaacgct ttttaagtcc
    30961 tcgctgcgct gcggggcttc cggcgcaaag cgcctcccat gcccttacag ggcataaaaa
    31021 caagggcaat taagcccctg cgcgatgatt gctgacttct ttttccagag ccttattaac
    31081 aaaggcattc aacgataaat cttcttccat cgccatttct gctacccgac gatgcagttc
    31141 tggatcaagc ctgacgttaa acacgccttt aaacggggta tcaggttcct ttccatcctc
    31201 cacacaagac tgtaaataca gatcaacaga tgttttaaac tcctgttcca gttcagctaa
    31261 tgtagaagcc tcataagtta ccaggtcccg aataaacgcc agttttccat acaggacatt
    31321 attttcaaaa tcgggttcaa ccgtgcctaa atatccttta tattttaaat gtttcataat
    31381 aatccagcct ctttcagatt ctgtttaatt gctttcagcg taccaccttt gatataactt
    31441 tccggatgtg gacgatgcat taatatggtg tgattgattt cagcattgaa aaaccgcact
    31501 cttgacccct gcatttcctt tttgacatat cccaaagagg aaaacaaaac gaccagttca
    31561 tcccattcaa acgttttttt actgtttaaa aactttgcca gtagcttatc tgttttcccc
    31621 ataaatccat cccctataga tatgcaacta attatagtta cacatggcat ttttgtcaaa
    31681 aaatacaaag gggcagaaag cccctttttt tatttaaaac atttcatacc cttgcaagta
    31741 gtttaaccac caccggaatt gatcccattc gctaaatttc attgccggaa ttctggtttc
    31801 ccagcctttg acaagggaat cagcaagctg tacgatctgc cgcgttgaca tttaaaaacc
    31861 tcattcagtt ttgattgtcg ataacgtgat caggttggtg gaacaacctg atcacacctt
    31921 gaatagtcag tcattaccct ccttcttttc tatagttact gtgtatccgt aaagagtcaa
    31981 aatgcgataa gcatccaacg cctcacgctc acttttaaat gttctgactt cgtgtaatcc
    32041 tttaccttta tcaagggata cacaaaactc ataatcttct tctacttcta tctttttaat
    32101 taatttatca agttcggcta atgttcgttt tggctcaaaa agaaatgtgc attgtacaat
    32161 ggcgtttaaa tcttcccgat caagctctaa cagagcatca ttataagcag ctttaaaaca
    32221 gcgattatta cgcagatcag atgcaaactg cattgcggct tgttttaacg ttttcatgaa
    32281 tttcccttgc attgatgtta atagacaatt tcagaatcaa tattgaaatt tccagaaaat
    32341 gcagggatgc ccctgcattc ataatgctta acaaagacac tcaataaaat ctttaaaact
    32401 caagcattca tcgccatctt ttaatgaatc aaaatatttt tcatattcgt ccctgtagaa
    32461 aaggactaaa tcaacatcac tctgcttaag ttcgccaaac tgaggaacaa ttccggcttt
    32521 atagtcacga attttattgt tatcagtaaa tatgacaaca tactgttgtc cagtatcctt
    32581 atgcataagg ataatagtgc aaagcgttac tcgttcgaat gtgaaaacat gattagtggt
    32641 tgaagtaaaa acagatgagc atttcatttt aaatctcctg ccctgctggg ctattgtgag
    32701 aggggccatt cccctttcga tgaaacaata atacgacctt taagtcgtat ttgcaagttt
    32761 tctcccgttt ttttactcga taagtgattg ttatcacgtt ttttaatccg cgtaagcgga
    32821 tggaaggggc gaacgaaaat accagggcag cacaggagcc acatcgcccc gaagcgcggc
    32881 gcagccgctg acgaacgtac cagggtgcac aggccatccc ttcaccctgc aaagcccaac
    32941 tgaccagcag gccatgatta tgctgatttg cgccgcagaa tgccgcagtt agcgcgagcg
    33001 caagcgagtt taccccgccc tggtgcattg cagcatggcg gggcgctgta ctgcgcgtgt
    33061 gagcgtcagc gagctacgag cgcaactcca ggtactaata taaaaccatc acaggtcatt
    33121 ttattttgac tcccttttgc agagcacgta ttcaggatgt tctcctgcaa gtaacaaaag
    33181 aaactcaaac tcaaccagtg acagactttg actgttttga gtattaactt cctctttttt
    33241 ctgccaggtt cttagcccaa caccataaat atgactgcat tccttttgtg ttaatcctgc
    33301 ttttaaacgt aattttttta cattttcagg tgtattatcg ggtttcattt aacataaact
    33361 ccttaattaa attcttaact ggcgtctcat cgtcttccct taatccggca cacgtaaaat
    33421 caatatgttg taattcattc tcattcagta cagcatcata caatgtaatt ttcgtagttt
    33481 tgttcccgat tttaacattt gttctggctt ttatcctttt tgtaagcctg taattgtcac
    33541 cttctttttc aagaaaataa gtcacatcac ctttattttt tctcataact gtttcaaata
    33601 ccctgtaatt ttaacaagtt gatctatact caagccccga atctgtaaat cataactgtc
    33661 gccctgttcg gtgactttac cgttcaatcc gttttcagct ttaaagcctg tcaatgcccc
    33721 ataccagctt tcaagctgga caagttgcga taacatacct ttcaggatca gaatttcgtc
    33781 ctttttttcc acctggtgtt tctcatactc aatcgcctgt aacacctcct gcgaagcatt
    33841 taccagaatt tttcgctcga tatcggctgc aatatcaacg ggattccgtt ttctggataa
    33901 cgtgcaaaca tgatgcggac tacgccagga acgactatcc acactcccca ccacagaaaa
    33961 gcggtctttt tccatccgaa tatgcacaga gtaattttta aactgcggtg acgtcagttt
    34021 tatacgagac gaacaatcat caagtttgtt cacgcgccag ccgttcccca ggatacggca
    34081 gacaacttca aagacagggt gatattgttc aaaaaaagag ttcatctgtt tccctttttg
    34141 ctacgtggct gctgttcccg tctttttggg ttcgatgccc caaaagacgg gctggctata
    34201 atatcggggg cttctcgccc ccgttctggt tacgctctgg tttccacgcg ttgccaccct
    34261 gtcgccctgc gctgtccggt tgtaccgtat tttgcgcgga tttcttctat cgtgtgttct
    34321 tcacgattcc agtaactttt atgttcgtct ggacgataaa accattgttt tttctttgcc
    34381 gcccatttac acccgatttc ttttaaagtt tccttatgtg taatggtttc tccactaatc
    34441 cagacccagt tacctatcac ttcaaatacc agaccagata aaccagaaag aacgtttaat
    34501 actttttcca ggtcatcacc gtaattatat cgtgcatgtt catcagtgct ctggaactga
    34561 tttattttat caatatttgc catcaataca tcaaaagctg catttaccgc tttcatcagt
    34621 tcagccccta acgggttac
//
