LOCUS       NZ_OR551296            14571 bp    DNA     circular CON 17-MAR-2024
DEFINITION  Acinetobacter junii strain 16 plasmid pKO16_MAC, complete sequence.
ACCESSION   NZ_OR551296
VERSION     NZ_OR551296.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Acinetobacter junii (Acinetobacter grimontii)
  ORGANISM  Acinetobacter junii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 14571)
  AUTHORS   Ko,S.Y., Rana,M.S., Kim,S., Kim,S.-Y., Kim,N., Lee,D.E., Hwang,H.R.,
            Kim,Y.K., Kwon,K.T. and Lee,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2023) Microbiology, Kyungpook National University,
            Jung-Gu, Daegu 41944, South Korea
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: De novo v. SPAdes 3.13.0.
            Sequencing Technology        :: Illumina; PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_033550345.1-RS_2024_03_17
            Annotation Date              :: 03/17/2024 10:01:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 13
            CDSs (total)                 :: 12
            Genes (coding)               :: 12
            CDSs (with protein)          :: 12
            Genes (RNA)                  :: 1
            tRNAs                        :: 1
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OR551296.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14571
                     /organism="Acinetobacter junii"
                     /mol_type="genomic DNA"
                     /strain="16"
                     /isolation_source="Urine"
                     /db_xref="taxon:40215"
                     /plasmid="pKO16_MAC"
                     /geo_loc_name="South Korea"
                     /collection_date="08-Dec-2015"
     gene            join(12128..14571,1..88)
                     /locus_tag="SG221_RS00065"
     CDS             join(12128..14571,1..88)
                     /locus_tag="SG221_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_318342607.1"
                     /translation="MHIKFLDHGKGSAAKASAYVLDKLDHMGNVRAGVEVLRGDATTFN
                     AICDSSPHLWKYTSGVIAWAKDDAPTDEQIKEVLNDFEQHAFAGLDPSQYHLFAVLHTD
                     DDGSKHIHVLTPRLDIESGKSLNIAPPGHEKHFDSLRDYFNTKYEWSRPDDFLLMRTTQ
                     EPNHIAKLNAQAKNILSSQELENLPKKQFCQAMDNYVQTLLKTQTAENRADIVACLKQL
                     DGIDSIKPSKDFLTVTLSSGKKHRLKGDFYNEKFEIGSYSEHLRAAAENRATANELAAA
                     LTDADQLRATYRTKRETYHRRHHAFAQRTADNNSPRIAPTLDFDSDKESITPSNTNAHN
                     ELYGANTSITSTAMQYRYIEHRNRHNQHRFVFNINPDVTGRNQQPVGKHHDRSREPAAE
                     YPRPDPTRNSEPKRAVPERHPDLNHGKIERTEIQIHFLGDDRFWTADSFNRFISGLSNQ
                     YKSEDHRSTARSHSTEHTTNFKSNQNLESIAEISNADRNRPFFERAKRLIDSTKQLVSR
                     AKQRIESTSEFIKKHLEQIQRSRAELTKQNQQLEYRERNPAGLDPGYEIRTFKTRTGQL
                     FGAITASISKQCADAITDAINESIKSTGFKQRCQRFSEDRIETHSFEYQHKADRSQERT
                     LENLASEGTRQLLQRSKDAEQANRHARGDSGNISQINQQLGGLEKTVAKIRLKPKPTTD
                     FYLNLLRNDNYYPDYVLYHKELCDEQQQAFNNQDTLLLIDYTQKKAENLETYMSRASRN
                     LNKSDYEKIEQIIKNDQRMLKYLQCEKILERQDTRFEQHRAAYQSCLKRFENIKLDIEQ
                     IKNSSSQHNVSQNTYEPEYKTKPKNDLDFNF"
                     /name="mobilization protein"
     gene            1106..2023
                     /gene="repM"
                     /locus_tag="SG221_RS00005"
     CDS             1106..2023
                     /gene="repM"
                     /locus_tag="SG221_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021644.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_151676400.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARGSGRGINANDP
                     LEVHAESYVNQFNVARQTAYQALKDACKDLFARQFSYQEVNKRGNIENVLSRWVSEIRY
                     IDDEATVKLIFAPAIVPLITRLEEQFTKYELQQISDLSSAYAVRLYELLIAWRSTGQTP
                     VIELAEFRQKIGVLDDEYTRMGNFKDRVLNLAITQVNEHTDINVKCEQHKKGRNISGFS
                     FTFKQKKVITSKSSTSLELFSKMTDAQRHLFANKLSELPDMNKYSQGTESYPQFAVRIA
                     EMLKNKEKFEELLPHLKKVGFSTK"
                     /name="repM"
     gene            2108..2641
                     /locus_tag="SG221_RS00010"
     CDS             2108..2641
                     /locus_tag="SG221_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_337250598.1"
                     /translation="MDASKAFKKSRTTIYEAIKTGELSRDHDGLIDLSELIRVYGNPIG
                     VQSSTRTEHAHKDVHVHVQSEIEHVLKDQISLLKNQLDLANQREKSLMQHIEDLTHRIE
                     FKGQLEQSKTELDERKVTTHSEMTGNSRPEPEPKHDGLTTPEQRENKRIPVPDHVEPEQ
                     PKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            3244..4719
                     /gene="msr(E)"
                     /locus_tag="SG221_RS00015"
     ARG             3245..4716
                     /name="msr(E)"
                     /gene="msr(E)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(E)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4775..5659
                     /locus_tag="SG221_RS00020"
     CDS             4775..5659
                     /locus_tag="SG221_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000155092.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWLL
                     RIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAETY
                     EIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLVKSE
                     IGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAHIDDP
                     AIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFALETQNE
                     SLIVGAKAQLGVI"
                     /name="Mph(E) family macrolide 2'-phosphotransferase"
     gene            complement(5849..6460)
                     /locus_tag="SG221_RS00025"
     CDS             complement(5849..6460)
                     /locus_tag="SG221_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_015060246.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKA
                     NEAKHKRIKELLVVGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            complement(6646..7818)
                     /gene="blaMCA"
                     /locus_tag="SG221_RS00030"
     CDS             complement(6646..7818)
                     /gene="blaMCA"
                     /locus_tag="SG221_RS00030"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCA family class C beta-lactamase"
                     /protein_id="WP_086374409.1"
                     /translation="MIFNQKYFFQASFITMVMGFGMNLHASEVSQDEKNIEKVVTQSFK
                     PLMDEYGVAGMAIGVIYKGKSYEKYYGVRFKDTNESVNGQTLFELGSLSKTFTAISGTY
                     AKNQGKISFNDQPSKYIPALKNSEINKVNLLELLTYTSGNLPLQFPDDVKTDKQILEYF
                     KNWKANNPPGAYREYSNPSIGLFGYLTAKSMNVSFSSLLEEIIFPQLNLKHTYVNVPEE
                     QKMNYAFGYDENNKPIRVNPGPLSDEAYGVKSTLPDMLKFVKSNLNVDTNSPAMKKAIL
                     DTHKGYFKVADSGMTQALGWEMFSYPTTSEILQASNSKQILLGLNPVVKELSQPKSKVF
                     HKTGSTNGFGAYILFIPEEDFGLVMLMNKKIPNVDRIKAAYNVFEILKNN"
                     /name="blaMCA"
     gene            complement(8405..9187)
                     /locus_tag="SG221_RS00035"
     CDS             complement(8405..9187)
                     /locus_tag="SG221_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_318342604.1"
                     /translation="MIEEIFARNLKKIRDHKNLSQEELAEKCGLDRTYIGILERREKIP
                     TLTTVEKCANALGISVCDLLSDEVYLNSFDDDKAVLNAIWPFICKYQDLATKNGINDIF
                     QDNGGKLLQVLLELDLTVLEGREGNDAKDATGQEYELKSLNVELVKGFSTHHHMNPTII
                     AKYRQVPWIFAIYRNIELQAIYRLEPDDLEEMYVKWEEKWHRDGGKDINNPKIPLKYVM
                     EKGTLVSGNAPAIPSKTKKKQTLENDSLVDGGFSPDND"
                     /name="helix-turn-helix domain-containing protein"
     gene            9405..10379
                     /locus_tag="SG221_RS00040"
     CDS             9405..10379
                     /locus_tag="SG221_RS00040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002059873.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_318342605.1"
                     /translation="MAEFQKPAYITELGESYIGNSLELLETLEDESINLVMTSPPFALQ
                     RKKAYGNEDQAEYIDWFTQFAVLVKKKLKPDGSFVVDFGGAYMKGVPVRSLYNFRVLIK
                     LVDEVGFHLAEDFYWFNPSKLPSPIEWVNKRKLRVKDSVNTVWWFSKTEFPKSDITKVL
                     APYSDRMKKLIEDPEKFYEAKERPSGHNIGKSFGKDNGGSIPPNLLQIPNSEATSLYLR
                     RCKQIGIKAHPARFPSKLPEFFIKMLTDEGDLVVDIFGGSNTTGYVAEQLNRRWKSFEL
                     SNEYVAASTLRFLPDSSTEEDLKETYENVLQGQSVSLNDPTAF"
                     /name="site-specific DNA-methyltransferase"
     gene            10476..10552
                     /locus_tag="SG221_RS00045"
     tRNA            10476..10552
                     /locus_tag="SG221_RS00045"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10510..10512,aa:Met,seq:cat)
                     /name="tRNA-Met"
     gene            complement(10679..10903)
                     /locus_tag="SG221_RS00050"
     CDS             complement(10679..10903)
                     /locus_tag="SG221_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_114271121.1"
                     /translation="MPYEEFQRLIGKSGLSIKEFAALLDMNANSITNYKKNGKVPTTIA
                     VIAVVISDMKDDGLDFYPIFEKVRAYRDQ"
                     /name="XRE family transcriptional regulator"
     gene            11052..11297
                     /locus_tag="SG221_RS00055"
     CDS             11052..11297
                     /locus_tag="SG221_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058952651.1"
                     /translation="MNHFVQPKLLISSQAKKIGLALINDLELCITARLAFYLDVNPSYD
                     GHEATFIWIEYFLQDHPQVEFSEIKEAFLKLIPECC"
                     /name="hypothetical protein"
     gene            11650..12138
                     /gene="mobC"
                     /locus_tag="SG221_RS00060"
     CDS             11650..12138
                     /gene="mobC"
                     /locus_tag="SG221_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005025066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_318342606.1"
                     /translation="MRSRTKIFRLTELQLEQLEQQLKKENTNFTDFIHSLIEREVMQNA
                     ITVQQHPVEIKPLRTKVKTVVEVVKRYQKTDPALLLELAKIGNNINQIARALNIIKNAD
                     PQEQRKLDIFSILLVLKAIQTELEQVFPALPKISRQSPEQLQKRLSSIQLSEEDESAY"
                     /name="mobC"
     ARG             4776..5656
                     /name="mph(E)"
                     /gene="mph(E)"
                     /gene_name="Mph(E) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3245..4719
                     /name="msrE"
                     /gene="msrE"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4776..5659
                     /name="mphE"
                     /gene="mphE"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1107..1794
                     /name="rep_cluster_2314"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.131"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctcgtcctct caacataatg tttctcagaa tacctatgaa cctgaataca aaacaaagcc
       61 aaaaaatgac ttagatttta atttttagtt aatctaaaca atgatttacc gagcgagtgt
      121 ctacgagcga ctgagcgata taaaaatttc gccctgctct ttctctgatt acaagcttcc
      181 ttgtgctgct aaaggagatt aaccgagcaa agcgacgact ctatatttga gtctagatcg
      241 agcgtagcga gtaaaaaagc tttatgagcg aagcgaattc cgagttgctt ttgctttctc
      301 ttaaagtcac gcgaataaat ttaattttta atttcatatg cacatgcttt tttaaaaaga
      361 gtttgtaatt tttgaatttc ttgtttgcat ttgtgatgta tttttataat ttgatattcg
      421 ttgttaatgt ataaacaaat tgagctttca ctaacagata ataaattatc actttctaca
      481 gtgaggcttt cttcattcaa aaaatgtaat gaaatacatg cattatcaca ataattaatg
      541 cttgaagata acagaatgta aaatttttca cttttgttgc agaaattcat atgagttcct
      601 tcttaaagaa tctgcataat atgaacttta gaaaaatgat gcgtaaatga actcatttaa
      661 gtggtaaaaa agctttatga gcgaagcgaa tgccgagttg cttttgctgt tgcctttgct
      721 ttttctcaaa ttcacaatga aaagaaccaa aaaattgccc cgacgaatcg agcgaaagcg
      781 agattcaata gagtttgagc gtagcgaaaa ccaagggcaa tttttcattc ccagtgcttt
      841 taattatttt taagttttta aatgctttta gactagctga acacctttat tttaaaggcg
      901 tgtagagctt atatgactac gtttacctac caatatgact acgtttacct accaatatga
      961 ctacgtttac ctaccaatat gactacgttt acctaccata aatcttcaaa atattttttg
     1021 tagatttaac tacgtttatc ttgcataaat ctacataatt attaatatag atttatattc
     1081 atgaaaaata atgtagacaa ataagatgcg ggatttagtt gttaaagata atgctctaat
     1141 aaatgctagc tataatttgg atttagtaga acaacgttta atcctattgg ccattgtcga
     1201 ggcaagaggt agtggcagag gtatcaatgc aaatgaccct ttagaggttc atgcagagag
     1261 ttatgtaaac cagtttaatg ttgcacgaca aacagcatat caagccctca aagatgcgtg
     1321 taaagactta ttcgcacgtc aatttagcta tcaagaagta aataaaagag gaaatattga
     1381 gaatgtccta agtcgctggg tcagtgaaat tagatacatt gatgatgaag caacagtaaa
     1441 attaatattt gcacctgcaa tcgtgcccct tattacacgt ttagaagaac aattcacaaa
     1501 atatgagcta cagcagatta gtgatcttag tagtgcttac gctgtacgtt tatacgaatt
     1561 attgattgct tggcgtagta ctggccaaac acctgttatt gaactagcag aattcaggca
     1621 gaagataggt gttctcgatg atgaatacac tagaatggga aattttaaag atcgagtatt
     1681 aaatctagct attacccaag ttaatgagca cacagacatt aacgttaaat gtgagcaaca
     1741 taaaaaaggc cgtaacattt caggattctc tttcacattt aagcaaaaga aagtgatcac
     1801 ctctaaaagt tcaacttcac ttgaactttt ctcaaaaatg accgatgcac aacgtcacct
     1861 ttttgctaat aaactttctg aacttccaga catgaataaa tattctcaag gtacagaaag
     1921 ctacccacaa tttgcagttc gaattgctga aatgctaaaa aataaagaga agtttgaaga
     1981 gcttctgcca catcttaaaa aagtaggatt tagtacaaaa taaacttcat gttcaggtag
     2041 gatattcaga tgaacatcct tgtacacgca cagagggatt ccattgaaca caacaaaatt
     2101 cagcgttatg gacgcgagta aagcttttaa aaagtcacga acaacaatat acgaagctat
     2161 aaaaactggt gaattatcaa gggatcatga tggactcata gatttatctg aacttatcag
     2221 agtttatgga aatcctatcg gtgttcagtc cagtacacgt actgaacacg cccataagga
     2281 tgtacatgta catgttcaat ctgagattga acatgtattg aaagatcaga tttctctact
     2341 caaaaatcag ttagatctag ccaaccaaag agaaaagtct ttgatgcagc atatagaaga
     2401 tttgacccac agaattgagt ttaaaggtca gcttgaacaa tcaaagacag agctagatga
     2461 acgtaaagtt actactcatt ctgaaatgac aggaaattct cgaccagaac cagagccgaa
     2521 acatgacgga ttgactactc cagagcagag ggaaaataag cggattccgg tgccggatca
     2581 cgttgaaccg gagcagccga aaagaggctt ttggagccgt ttttttagac cctatgatta
     2641 accacgattg tttaactgat atgaacaaag tcgtgaaaca ttgctcaaag aataggcgtg
     2701 gaacgtgaaa aatcataaaa aaagcccacc ttggggaagg tgagctatca actagaaact
     2761 acagcattga tttctataaa taattataac gcatttcgta taaggtgtat tatgttaatt
     2821 ttagaaaatc ttcactacta ttattttaaa ataagcctta tatccaagca taaaacaagg
     2881 ttgtctagac ttcttttaac agtaaagtta tcataaaact gaattttatt ttttaggtaa
     2941 gtttatgcat tctatccgca ttcgttaaga cacaactatt tgcatagtga cactatttta
     3001 taatggtggg cttttgttgt gtctttaaga atatatgcgg atatataaag taaaagtatg
     3061 cttaatttat aagtatgctt ttagtgcata gtttccagtt ataacttaat tgactagcta
     3121 tttgtccacc ctgtggatga atagcttttt ttttgggagg acactgtgat gctagctttt
     3181 gttttcacct aaatcctgtt tgctgcataa aaaatttcaa gagctaaaca ggagtaaata
     3241 aaaatgagtt taattattaa agcgagaaac atacgcttgg attatgctgg gcgtgatgtt
     3301 ttggatattg atgaattgga aattcactct tatgaccgta ttggtcttgt gggtgataac
     3361 ggagcaggaa agagtagttt actcaaagta cttaatggcg aaattgtttt agccgaagcg
     3421 acattacagc gttttggtga ttttgcacat atcagccaac tgggcggaat cgaaatagaa
     3481 acggtcgaag accgggcaat gttatctcgc cttggtgttt ccaatgtaca aaacgacaca
     3541 atgagtggcg gagaggaaac tcgtgcaaaa attgctgccg cattttccca acaagtacat
     3601 ggcattctag cggatgaacc aaccagccac cttgatctca atggaataga tctacttatt
     3661 ggtcaactta aagcatttga tggagcatta cttgttatca gtcatgaccg atattttctt
     3721 gatatggttg tagacaagat atgggagtta aaagacggta aaattacgga atattggggt
     3781 ggttactcgg attacttgcg tcaaaaagaa gaagagcgac aacaccaagc cgtagaatat
     3841 gagctgatga tgaaggaacg ggagcgatta gaatctgctg tgcaagaaaa acgccagcaa
     3901 gctaatcgat tagacaataa gaaaaaagga gaaaaatcca aaaactctac cgaaagtgct
     3961 ggacgacttg ggcatgcaaa aatgactggc accaagcaaa gaaaactgta tcaggcagct
     4021 aagagtatgg aaaagcgttt ggctgcatta gaagatattc aagcaccaga gcatttgcgt
     4081 tctattcgtt ttcgtcaaag ttcagcccta gaactgcaca ataagttccc gattacggca
     4141 gatggtctga gcttaaaatt tggtagccgt actatctttg atgacgctaa ctttataata
     4201 ccgcttggcg ctaaagtcgc tataactgga tcgaatggaa cagggaaaac gtccttgtta
     4261 aaaatgatat cagaacgtgc tgatggatta accatatctc caaaagctga aattggctac
     4321 tttacacaaa caggatataa atttaacacg cataaatctg tgctctcctt tatgcaggaa
     4381 gagtgcgagt acacagttgc ggaaattcgt gcagtattgg cttcaatggg gatcggagcg
     4441 aatgatattc aaaaaaactt atccgactta tcgggaggtg aaatcatcaa actgctttta
     4501 tccaaaatgc ttttaggaaa atataatatt ttgcttatgg atgaaccagg aaactatctt
     4561 gacctaaaaa gtattgccgc attagaaaca atgatgaagt cctatgcagg aactattatc
     4621 ttcgtatctc atgacaagca attggtcgat aatattgctg acattatcta cgagatcaaa
     4681 gaccacaaaa tcatcaagac ttttgagaga gattgttaat gatagccaat ctaatccgaa
     4741 cattaattat tgaactcttt aaaggaaatt aaaaatgaca attcaagata ttcaatcact
     4801 tgctgaagca cacggcttgt tgcttacgga caaaatgaat ttcaatgaaa tgggcattga
     4861 ttttaaggtc gtttttgctc ttgatacaaa ggggcaacaa tggttgctgc gtattcctcg
     4921 tcgtgatggc atgagggaac aaatcaagaa agaaaaacgc attttagaat tggtaaaaaa
     4981 acatctttct gtagaggttc ctgattggag aatttcatct acagaattag tggcttatcc
     5041 catacttaaa gataatcctg ttttaaattt ggatgctgaa acctatgaaa taatttggaa
     5101 tatggacaaa gatagcccga aatacataac atctttggca aaaaccttat ttgaaatcca
     5161 tagtattcct gaaaaagaag ttcgggaaaa tgatttgaaa attatgaaac cttcagattt
     5221 aagacctgaa atagcaaaca atttgcagtt agtaaaatct gaaattggta taagtgagca
     5281 attggaaacc cgctacagaa aatggttgga taatgatgtt ctatgggcag atttcaccca
     5341 atttatacat ggcgatttat atgctgggca tgtactagct tcaaaggatg gagctgtttc
     5401 aggcgttatt gattggtcaa cagcccatat agatgaccca gcgattgatt ttgctgggca
     5461 tgtaactttg tttggagaag aaagcctcaa aactctaatc atcgagtatg aaaaactagg
     5521 gggtaaagtt tggaataaac tatatgaaca gactttagaa agagcagcgg cctctccttt
     5581 gatgtatggt ttatttgcct tagaaactca aaatgaaagc cttatcgttg gagcaaaagc
     5641 tcagttggga gttatataat ttaaaaatat gattgctgag aactgccttg ttttgaaact
     5701 tggttggctt taattagttt ttagtattct ttatagaaaa tgcctcgatc aaggggcatt
     5761 tctaacaatc atttaacata aaatttctta tgtgaagtaa cctgaatgca gcggtgcccc
     5821 attgggggcg cgttgagata agcctctttt agataacttt agcaactcga tagacagttg
     5881 caactccaca attcactgct ttggcaattt cttccttagt catattgcca actactagca
     5941 attcttttat tcgtttatgt ttagcctcat ttgccttctt gcctgttggt ttgtaacccg
     6001 aacgtgccag gccctgctta atacgttcta tacgtttctc attgtctagg cgggccattg
     6061 ttgccagaag atcaatcaac atattgttga tgagttccaa gattgaatga gtaatgctgt
     6121 cactggtttg aatcatttgg taggtagtag gaagatctgc tacgactaaa cgaagtccct
     6181 tctcctggat cctgcgcttc agctcttgaa aatctcgttg ggttaggcgt gataagcggt
     6241 caatactttc aactaacaat gtgtcacctt gatttgcttc tgacagtagc ttattcaatt
     6301 caggtcggtc taacttcgta ccactatagt tttccacgta cacaatggtt tcacccaaat
     6361 tcaagttttg gtttagatcc tgaagaatct gcaaagccct ttctgcatct tgatctttag
     6421 ttgaagctcg tagataaata cgtgtattca tttctatcat ttaatcttaa ttctattaga
     6481 ttaatgataa tactatcatt taattattgc attaagtcta atgatagata ttgtatgcga
     6541 tttggctcgc tatcattcag gtatagtctt ttgataaaaa aagcatgact aaaagccatg
     6601 ccttgggaat tcgtataacg tgtattatgt taattttaga gaaagttaat tatttttcaa
     6661 tatttcaaaa acattatatg cagccttaat acgatctaca tttggtatct ttttattcat
     6721 taacataacc aatccaaagt cttcttctgg aataaataaa atatatgccc cgaagccatt
     6781 agtagatcca gttttatgaa aaactttaga ttttggttgc gacaactctt ttacaacagg
     6841 atttaatccc aataagattt gttttgaatt actagcctgt agaatttcgg aagtggtggg
     6901 ataagaaaac atttcccatc cgagtgcttg tgtcatacca ctatcagcaa ctttaaaata
     6961 tcctttgtgt gtatctagta tagctttttt catagcaggg ctatttgtat ctacattaag
     7021 gttcgacttt acaaacttaa gcatatcagg gagtgttgat ttaactccat atgcctcatc
     7081 cgacaatggg ccaggattaa ctcgaattgg cttgttattt tcatcataac caaaggcata
     7141 gttcattttt tgttcttctg gaacattgac gtatgtatgt ttcaaattaa gttgaggaaa
     7201 aataatttcc tctaacaatg aagaaaaaga gacattcatc gattttgcag ttaagtaccc
     7261 gaatagacct atacttgggt ttgagtattc acgataagcc ccaggaggat tgttagcttt
     7321 ccaatttttg aaatactcta atatttgctt atcagttttg acatcatcgg gaaattgtaa
     7381 tggtaaatta ccacttgtat aagttaataa ttctaataaa tttaccttat taatttctga
     7441 attttttaga gcagggatgt atttactagg ctgatcgttg aacgatattt tgccttggtt
     7501 cttagcatat gtacctgaaa tagcagtaaa agttttactt aaagaaccta actcaaaaag
     7561 tgtctgacca tttacgcttt catttgtatc tttaaatcga acaccataat atttttcata
     7621 acttttacct ttataaatta cgcctatagc cataccagcc acaccgtact catccataag
     7681 tggcttaaag gattgtgtaa caactttttc aatatttttc tcatcttgac tgacttcact
     7741 tgcatgtaaa ttcataccaa aacccataac cattgtaata aagcttgctt gaaaaaaata
     7801 tttttgatta aaaatcatta agctaaattt cttggaaaaa gatggtaaat ataatctcat
     7861 atactaaata tcaaatatgt attaattttt tttaaataat ttcttatata aaaatcatct
     7921 aatccaacgg ttatttaaca taatgggcgt tatacgaaac gcatataaaa atagttttat
     7981 atttcaaaaa gttatgctat aaaaaattgg ggacgcaaac ccgaaaagcc gacttggaaa
     8041 caagtcggct ttttattgag cggttttgat acttcttgcc aataaaatcg atcaaaaaac
     8101 attcgttttt gcattttttc ctgatttctc aagggtcaca gttcagaaat aatccccaat
     8161 ttctgcggac aactcttggg ataaaatagg ccaactttgt aagctaaaac ttacacagat
     8221 ttaaggtttt tgcggattgg taatccaagg cattagccct atgatgaata cataagggaa
     8281 ctggatgttt ccagaaaaca agaataagaa aatagcgcaa ggaatgggca gcacaagatc
     8341 agtaagacga gcaccccgat atgaaaaagc ccgacaggat gtcgggcttt tttattacct
     8401 aaatctaatc attatctgga ctaaatccac catcaaccaa tgagtcattt tctaaagttt
     8461 gttttttctt tgtcttagat ggaatagctg gagcattgcc actaactaga gttccttttt
     8521 ccattacata ctttaatgga atcttcggat tattaatgtc ttttccgcca tctctatgcc
     8581 atttttcttc ccattttaca tacatttctt ccagatcatc tggttctaaa cgataaattg
     8641 cctgtaattc aatatttcta taaattgcaa aaatccaagg aacttgacga tatttagcaa
     8701 taattgttgg gttcatatga tgatgagtag aaaacccttt caccaactcc acattcaaag
     8761 atttcagttc atattcttgg ccagtagcgt cttttgcatc attgccctca cgtccttcaa
     8821 gaactgttaa atctagctct aaaagcactt gcagaagttt ccctccatta tcttgaaaaa
     8881 tatcattgat accatttttc gttgcgagat cttgatattt acaaataaaa ggccaaatgg
     8941 cattcaaaac agctttgtca tcatcaaaac tattcaaata cacttcatct gataataaat
     9001 cgcagactga aatacctagc gcatttgcac acttttcaac tgtggtaagt gtgggaattt
     9061 tttctcttct ttccaatatt ccaatgtatg ttcgatctaa accacatttc tcagctaatt
     9121 cttcttgaga tagattttta tgatctctta ttttctttaa atttctggca aaaatctctt
     9181 caatcattac ttaaatccac attaattaac tgatatttgt ggaataaata atcatcgtta
     9241 gatcaacata tttcgaccga ctaaagatag catttataat aaaatatatt tttaaatgct
     9301 atctttagca aggatgacta aagtcatctt tttttattta tgttttgcta aatttaaagt
     9361 acccatgtgc taacatactc actttatttt ttcaggactt cttaatggct gaatttcaaa
     9421 aacctgcata tatcaccgag cttggtgaaa gttatatagg taattcactt gaacttcttg
     9481 aaactttaga agatgaaagt attaatttag tcatgactag ccctcctttt gccctacaac
     9541 gtaaaaaagc ttatggcaat gaggatcaag ccgaatatat tgattggttt acacaatttg
     9601 ctgtacttgt aaaaaagaaa ttaaagccag atggaagctt tgtagtagat tttggtggtg
     9661 cctacatgaa aggtgtcccc gttcgcagcc tttataactt ccgagtttta attaaattag
     9721 ttgatgaagt tggtttccat ttagcagagg atttttattg gtttaatccc tctaaactcc
     9781 ctagccctat tgaatgggtc aataaacgaa agttacgtgt aaaagattct gtaaatactg
     9841 tttggtggtt tagtaaaact gagtttccta aatccgatat tacaaaagtt ctcgcgccat
     9901 atagcgatag aatgaagaaa ctgattgaag atccagaaaa gttttacgaa gcaaaagaaa
     9961 gaccttctgg gcataatatt ggtaaaagct ttggtaaaga taatgggggc tcgattccac
    10021 caaatctctt gcaaatacca aattctgagg ctacatcact ttatctaaga cgttgtaaac
    10081 aaatcggtat aaaagcacac ccagcaagat tcccaagtaa gcttcctgaa ttcttcatta
    10141 aaatgctaac ggatgaaggc gatttagttg ttgatatatt cggtggttca aatacgactg
    10201 gatatgttgc agaacaatta aatagacgtt ggaaatcttt tgaattatca aatgagtatg
    10261 ttgcagcatc aacattacgt tttttaccag actcttcaac agaagaagat ctaaaagaaa
    10321 cttatgaaaa tgttctccaa ggccaatccg taagcttgaa tgacccaaca gctttttaat
    10381 taacctaatt agtttactta acaagctatt aaactataaa tattcagttt tctttgactt
    10441 ggttcatata agtaacgtat aacggcttcc attgatgcgg gatggagcag tctggtagct
    10501 cgtcgggctc ataacccgaa ggtcgttggt tcaaatccag ctcccgctac caatccagat
    10561 acgaaagcca atctttaaaa gattggcttt ttttatttaa cataaaatct catctacgaa
    10621 atgcagcagc acgcgctagg atccaattaa aactttaatt gggttcacat caaatactct
    10681 attgatcacg ataagctcga actttctcaa aaattggata aaagtccaga ccatcatcct
    10741 tcatatctga aattacgact gcaattactg caatcgtagt gggcacttta ccgttctttt
    10801 tatagttggt aattgaattt gcgttcatat ctaaaagcgc tgcaaattct ttaatagaca
    10861 ggccagactt acctataagt ctttgaaatt cttcgtaagg catagtttaa aaagcagaat
    10921 tgttctgatg gaaaagtata gcacagatca tttatttaca ttcaaacaca taaataatgt
    10981 gttaatcaca atatttatgt gtttaactat tcttatcaag cacttttact cttttgatag
    11041 gacttgtttt tatgaaccat ttcgtacagc ccaagcttct tatttcttct caagcaaaaa
    11101 agattggatt ggcattaatt aacgatcttg aactttgtat aacagcacga ttggcttttt
    11161 atctggatgt aaatccttcc tatgacggcc atgaggcaac attcatttgg atcgaatatt
    11221 ttttacaaga tcatcctcaa gtcgaatttt ctgaaattaa agaagcattt ttaaaactta
    11281 ttcctgaatg ctgctaaacg atgataatga aatctgtatt ttatgagtca aatacagatt
    11341 tcaaatctct ctttttacaa tcaatacgat taatttttaa ccaaatatag catccatttt
    11401 tagaacaaaa ataatagttt tatcccccca attttcccaa aaattcacgc cataattaaa
    11461 atttacccca tttttcgctt caaaaagact ttattttttc aatgctgata taacgagttc
    11521 agaccctagc acgtgaccca agcaagccta cctgtatata caaaattctt cgaatttcga
    11581 tacacaggga cttgttaggg gtttcacccc taagacccca aaagcaaaag tgcgcgatga
    11641 gattgatcaa tgcgaagtag aacgaaaatt ttcagactca ccgagctaca gttagagcaa
    11701 ctggagcaac agctcaaaaa agaaaataca aatttcacgg acttcattca ctcgttgatc
    11761 gagcgtgaag tcatgcaaaa tgccatcacc gttcaacagc atccggtaga aataaaaccc
    11821 cttagaacaa aagtaaaaac agtcgttgaa gtcgtcaaac gctaccaaaa aactgaccca
    11881 gcgttattgc tcgagcttgc caagattggc aacaacatta atcaaattgc acgcgcatta
    11941 aacatcatca aaaacgcaga tccgcaagag cagcgcaaac tcgatatttt cagtattctt
    12001 ttagttttaa aagccattca aacagagcta gaacaggtat ttccggctct tccaaaaatc
    12061 agccggcaaa gccctgaaca gctccaaaaa cggctgtctt cgatccaact atcggaggaa
    12121 gacgaaagtg catattaaat ttcttgatca cggcaaaggc agtgccgcca aagcatccgc
    12181 atacgtactc gacaagcttg atcacatggg gaatgttcgc gcaggcgttg aagttttaag
    12241 gggggatgca actacgttta acgcgatttg tgactcaagt cctcacttat ggaagtacac
    12301 cagtggagtg attgcatggg caaaagatga tgcccctacg gacgaacaaa tcaaagaagt
    12361 actcaacgac tttgaacaac atgcctttgc aggtttagat ccatcgcaat atcacctttt
    12421 tgctgtgcta catactgacg atgatggatc aaagcatatt cacgttttaa cgcctcgact
    12481 cgatatcgag agcggaaaat cactgaatat tgcaccgcca gggcatgaaa aacatttcga
    12541 ctcgttacga gactacttca atacgaaata tgagtggtca cgtcctgatg actttctttt
    12601 gatgcgaacc acccaagagc caaaccacat cgcaaagctg aatgcacagg caaaaaatat
    12661 tctttcaagc caggagcttg agaacctgcc aaaaaaacag ttttgccagg cgatggataa
    12721 ctacgttcaa acactcttaa agacccaaac ggcagaaaac agggccgata tcgtcgcttg
    12781 tctcaaacaa ctcgacggca ttgattcgat aaaacccagc aaagattttt taacagtcac
    12841 tctctccagt ggaaaaaaac atcgactcaa aggcgatttc tacaatgaaa aattcgaaat
    12901 tggatcttat tcagagcatc tccgagcagc agcagagaac cgagcaactg caaacgaact
    12961 tgcagcagct ctcacagacg ctgatcaact tcgagcaact tatcgcacaa agcgagaaac
    13021 ctatcacaga aggcaccacg cttttgcaca acgtacagca gacaacaaca gccctagaat
    13081 cgcacctacg ctcgattttg atagcgataa agaatctatc accccaagca atacaaacgc
    13141 tcacaacgag ctatacggag caaatacaag tattacaagc acagctatgc aatatcgata
    13201 tatcgagcat cgaaaccgtc acaaccagca tcgattcgtt ttcaacatca atccagacgt
    13261 tacaggaaga aatcaacagc cagttgggaa acatcacgat cgatcaagag agcctgcagc
    13321 agagtatcca agaccagatc caacacgcaa tagcgaacca aagcgagcag ttccagaacg
    13381 acatccagac cttaatcacg gaaaaattga gcgaacagag atccaaatac attttttggg
    13441 ggatgatcgg ttttggactg ctgattcttt caatcgtttt atatctgggc tatcaaacca
    13501 atacaaatct gaagaccatc gaagcacagc gagaagtcat tcaacagaac acacaacaaa
    13561 tttcaaatca aatcaaaatc ttgaatcaat tgcggagata agcaatgcag acagaaatcg
    13621 accttttttt gaaagagcaa aacgacttat tgattcaaca aaacaacttg ttagcagagc
    13681 aaaacaacgt attgaatcaa caagtgagtt cattaaaaag catcttgagc aaatacagcg
    13741 aagtcgagca gagcttacaa aacaaaatca gcaacttgaa taccgagaga gaaacccagc
    13801 aggccttgat cctggctatg aaatcagaac tttcaaaact cgaactgggc agcttttcgg
    13861 agcaattaca gcaagcattt caaaacaatg tgctgatgca attacagacg ccattaacga
    13921 gagcattaaa tcaactggat ttaaacagcg ttgtcagcga tttagtgaag accgaattga
    13981 aacccattct tttgaatatc aacacaaagc agaccgatct caagaacgca ctcttgaaaa
    14041 tctcgcaagt gaaggtaccc gacaactttt acaaagatct aaagatgcag agcaagcaaa
    14101 tcgacatgct cgtggagaca gtggaaatat tagtcaaatt aatcaacaac ttgggggact
    14161 agaaaaaaca gtagccaaaa ttcgacttaa gccaaagcca acgacagact tttatctcaa
    14221 tttattgcgc aatgacaatt attaccccga ctatgttctt tatcacaaag aactatgtga
    14281 tgaacagcaa caagccttca ataaccagga cacattacta ttgattgatt acacacagaa
    14341 aaaagcggaa aacttagaaa cctacatgag ccgagcaagt cgaaacttga ataaatctga
    14401 ctatgaaaaa attgaacaga tcatcaaaaa tgatcaacgc atgctgaaat atctgcaatg
    14461 cgagaagatt ttagaacggc aagatacccg ttttgagcag cacagagctg cataccaaag
    14521 ctgtttaaag cgctttgaga atatcaaact tgatattgag caaattaaaa a
//
