LOCUS       NZ_OR345922             4082 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Escherichia coli strain E2022 plasmid pE2022-2, complete sequence.
ACCESSION   NZ_OR345922
VERSION     NZ_OR345922.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Zorea,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2023) Life science department, Ben Gurion
            University, David Ben Gurion Blvd 1, Be'er Sheva 8410501, Israel
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Megahit v. v1.0.3
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 02/12/2024 13:09:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OR345922.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E2022"
                     /isolation_source="ESBL clinical isolate"
                     /db_xref="taxon:562"
                     /plasmid="pE2022-2"
                     /genotype="ST131"
     gene            join(2334..4082,1..411)
                     /gene="mobQ"
                     /locus_tag="V5G32_RS00005"
     CDS             join(2334..4082,1..411)
                     /gene="mobQ"
                     /locus_tag="V5G32_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(667..1368)
                     /locus_tag="V5G32_RS00010"
                     /old_locus_tag="pE2022-2_00002"
     CDS             complement(667..1368)
                     /locus_tag="V5G32_RS00010"
                     /old_locus_tag="pE2022-2_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1901..2155)
                     /gene="mobC"
                     /locus_tag="V5G32_RS00015"
                     /old_locus_tag="pE2022-2_00003"
     CDS             complement(1901..2155)
                     /gene="mobC"
                     /locus_tag="V5G32_RS00015"
                     /old_locus_tag="pE2022-2_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2157..2333)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          2334..2692
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(668..1368)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2248..4082
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.673"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaccgggaaa cccgccagct tcagcttttg ctggtcatgg gcgtggatga ctctctgggc
       61 ggtgttgtcc ggcttaatgg tacgctgtat cccgcgtttg ccgttccctc agccgataac
      121 agccagctgg tcatcagtgc actgaccgat aaggggttac ggtatgcagg gtatggtgtt
      181 gccgtaaatc acgatgcaga cagccacata tcacctgcgc cggaacttat ggagtttcat
      241 ctgaaaaccc gtgaggcacc gctttttgct gccgtcaaca caccagaaaa acagccggat
      301 catctgttcc gctcgctggg ttttaatcgt acctgggacg aatggcggcg cgaggaagat
      361 gcacgaacac acgccaccga gaggagacat gatcgcggtt ggtcacagtg aatgtgaaca
      421 atattttgtt ttgtttaaca tcgtgttatg ttcgtcgatg ttcttcgatg ttcatcgaca
      481 gggatgaatg caagaagcac acacataatg caagcatagt tttgtttttc cgcccccaaa
      541 aaatgggggt tcttttttcg ctgcattaga gcgcttttga cgcaattttt cacttcaata
      601 gtaccttcgc actactaaca caaaaaaaac gcagcaggga gcgttttagg tgccttaaaa
      661 tcgattttag atcgtcatct tgatttgttt gtcttccttg aagcgaaaat gcagggctac
      721 aaccgtgcgc ccctttttga tcgtctcaac cgttacagcc aggtcgcttt ttttgttcag
      781 ttcgtcaatg cagggtctta ataattgctg gttaagtgat ttatatgttt gatatttatg
      841 ctctatatca agcattgagc gaaaatcatc gagagcgata atacgttcgc cagtactgcg
      901 gaactgctgg aggagttcgt atatccttat actgtaactt cttgataatc tagatatatt
      961 cttgattaca actctagtaa attgcccttg tagttgtgtc aaataaggca ttactgcgtc
     1021 agaaaatgtt atttgtgctc tggcctcgcc tttagcgtac tgtgcccgat actggatcca
     1081 gcggaactcc tcacgtttct cgtcatcctt gagaatgatt gacctgtccc acaaacgatc
     1141 aattgcttcc tgtaactgaa cctcggcatt tttccgcccc atgtcaggga atgaatcgaa
     1201 atattccgca gctgtaatgg tcattgtctt ttgcaatttt ggagattctg catcagcccc
     1261 agacttcaat cgaccgatgc aaagtaacaa aaatcgctgt tcctgtaggg tcaatttata
     1321 actggcttca accagcgcat tggatttata ggcaattaga tccgacatag agcatccctc
     1381 cccaaaaggt aatgaataaa agataaacat accctcaacg aaaggcaatg cctttttatt
     1441 ttaatatccc tttttctgtg aatattacct taaatcatat tgcctttacc ttaaattccc
     1501 ccacctaaac accttaaatc atattacctt taccttaaat cacattacct atagcattgt
     1561 aacattttgt tttctataag gaattttggc gctaaaacaa gtattaaaaa caagatctta
     1621 aaaacaagat ctaaaaactt aacgacgagc ctgtggataa cttggagtgc tgaaaacaaa
     1681 aaaccaataa aaacaatgcg ttgcaaaaaa gcagaattgc aagttccgaa tacaccccct
     1741 ccaaccctgt ggaaatccag tattcatgcg ggttcagcgc atatcacctc ccgaaaaacc
     1801 gtgcgtatgt gctcaatcag gcgacggatt cacttccgct tcctcgccca ctcgctcgct
     1861 aacgctcggt cgctgggact gcggcgagcg gacgatacaa tcactgtcct ggcagcgctt
     1921 ccagctcgtt aatcagcggc agtatgcgcg tcacatcagc ctgtcgtgtc acctctgcct
     1981 tcaggaactg gagtaaccag gcacgcaact gcacctcctc cggctttctg acctttgcca
     2041 gcaatgctgc acccacaatg accttctgcg ctgtgtccag actcctgctc ttcgccttca
     2101 ggcgctgtaa tctggcctca gcatcagcaa tcttctgttc gagtgttctg ctcatgatgt
     2161 aactccgtgc gcgatgaaaa atcgcatttt agcgcgtcac tggtagttta aaaactgagc
     2221 tggcataatg cacggaaaat cacgaagtgc gcacttatac aatctccact ctgtttcgat
     2281 tgtgtgcgcc ctgcggggct gaaagaaaac cacaaaaagg cattacggca gaaatggcga
     2341 tttatcatct cagcatgaaa atcatctcca gaaacagcgg ctacagcgcc gttgcttctg
     2401 ctgcctatcg ttccggttca ctgatgcttg atgagcgtac cggactgact cacgactaca
     2461 cccgcaaaag cggtgtggct gaagctgtga ttctgactcc cgcgacagcc cctgcctggt
     2521 gtacgaaccg tgccgaactc tggaatgccg ttgagaaggc cgaacgccgg aagaactcac
     2581 aacttgcgcg ggaaattgaa ctcgccatcc cccgcgaact accgcaggat gccgcacggg
     2641 aaaccgttct cgcctttgtc agagaaaact ttgtcagtca gggcatgatt gctgatgtgg
     2701 cgttccatca catggataaa acaaatcccc atgcgcacat catgctgacc acgagagctg
     2761 tcggacctgc tggattcggt ggaaaggtca gggactggaa cgaccggaca catgccgaga
     2821 cgtggcgtgc atcatgggct gaccatgcga acagggcact ggcgaacgcc ggttatcagg
     2881 aagagataga ccatcgttca tacgagcgac aggggcttga gaaaacgcct ggtattcacc
     2941 tcggaaagtc tgcctgtgcg atggaaacac gcggtatcga aacagaacgc ggcgaacaga
     3001 atcgtctgat taacagactt aacctggaaa tacagatttc ccgtaccgaa ctcagaaaca
     3061 gcgggctgac agagcctgcc cgccgtgtgg ctgaccttct gaatatcgtg cttcctgaca
     3121 acgcaacttc gtacacacta cgtgacatca tcgaagcgtt gccgaaggac agtaatgcgg
     3181 catggaaact caccagtaat gccatgtcga tgatggcaga tatgcaggcg acgcgcagac
     3241 gctgggaaga gctgaacacc gaacgaaagg aagcgatgag ccatgctgcc agcctgaaaa
     3301 aatcatcgcc gttttcgtcc ggtttttccc gtattcccct gatgcaatgg gcggcaccgg
     3361 aataccgtag cgaacaggga aaaatccagt cgctgagcca gcaaatggaa aaattgcgac
     3421 agcattaccg gaaggtggag aaacaggaca ttcccgcgag tcagacggca tttgaaaaac
     3481 agtggcagca gtggggggca tccggccttg cagctctcag agtccagttg cttaaacgtg
     3541 aagaggaatt gcgccggaag gagcaggagg atacagaacg tagtcgcgcg gagcgacgag
     3601 cggaacagct caggaaaaat gacaatgccg ttatggattc aggggtactg agcgcagtcg
     3661 ttacggggta tggcgaggcc cccatgcctg gtaacgggga aatgacctgt taccttctgc
     3721 ttcacaaccg gaacggagaa tacacgctct gggggaacga gctggagaaa taccgcacca
     3781 gaatctttga gcgtgttgat ttaatgcgtg acaggagtgg ttatatctgt gaccgaagcg
     3841 aaatcggtca acatccacca ctgcaacgtg tttattcctc tgcgaccttt gagcaacttc
     3901 tgacgcaggt ttgccagaca tggccgcaat atacccgcaa tctccgccag ccgaagacat
     3961 ggccggagtc tttctgtctt ggagaagacc gccagccagc catgccatca ctggcagcaa
     4021 ggaaggtgga ttttacgcag ggacgtctgc taccgacgct gatgccggta atgagttctg
     4081 tc
//
