LOCUS       NZ_OR345921             1546 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Escherichia coli strain E2022 plasmid pE2022-1, complete sequence.
ACCESSION   NZ_OR345921
VERSION     NZ_OR345921.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Zorea,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2023) Life science department, Ben Gurion
            University, David Ben Gurion Blvd 1, Be'er Sheva 8410501, Israel
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Megahit v. v1.0.3
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 02/12/2024 13:09:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1
            CDSs (total)                 :: 1
            Genes (coding)               :: 1
            CDSs (with protein)          :: 1
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OR345921.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E2022"
                     /isolation_source="ESBL clinical isolate"
                     /db_xref="taxon:562"
                     /plasmid="pE2022-1"
                     /genotype="ST131"
     gene            162..638
                     /locus_tag="V5G31_RS00005"
                     /old_locus_tag="pE2022-1_00001"
     CDS             162..638
                     /locus_tag="V5G31_RS00005"
                     /old_locus_tag="pE2022-1_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1243..1324
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          complement(871..1128)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="99.0"
                     /evalue="3.93e-111"
ORIGIN
        1 tgttttttta gtgtgttttt tgtttacctg tcgcatccag ggtgacacaa cgttgtcgca
       61 tcctggatgc gacaacacgc aacaaggtgt tgcacagatg tgtcatgatg gcttagattt
      121 gaaccaacgt tagtgcatgg gattttcaga gggaaaaaat catgtttatt gattcagaaa
      181 aacgactgaa acaactttca gatgaggcaa agaaaaacac cgaggatctc gaagaagcaa
      241 agaagaattc aaggtttaca caggtatccc caaaaggttg ggaacgcgtt cgagagctgc
      301 tgaaggatag ccaaggcata tcagcactga agctgtactc atttttagcg gagcatatcg
      361 atcctacgtg tggcgctgtc gttgcggatc agcaattcct agctgaaaaa cttggagtta
      421 gcagaagcac aattattcgg tggctcaatt acttagaatc aaaaaatgca ttagttagaa
      481 tccccgttgc tggtaaggtt tgtgcgtatg ccctcgatcc acatgaagtc tggaagggat
      541 acaacactac gaaaaaccat gcagcgtttg tcactaaaac actggtcaac aaagacggtg
      601 atattcagcg ccgaatcatg gccatgtttt caaattgagc cagcggcagg cggacaatca
      661 ggggctacgt gttaacgttt tgaccatgat tgtctatcct gcattgctct tttgccgcct
      721 caaaatccct tgcgtgtttt tgctccccga tccccagaac gaccgcgccg ccacggttcc
      781 ggcagcgcct tgagcgcaac cggaaaccgt ctggggagaa ccccagaccc ccatcgctgt
      841 aacaccgatg gtctacgacc accaaccagc cgaatagctg cctgtgccgt gcaaataggc
      901 tttgcaggga gctgacgcgg ttcctagcga cctgaaagcc cctggttagg ggtatcggcg
      961 cgcctgacct cctgagagcc tctgtaagcg tttttcgcgc cttccatgcc ctggcgggca
     1021 tttaaacccg tttcgtgcgt ctgagagctt ctgagcgtgt ttgaagggct atctgacgac
     1081 tcatccccga aagaacaaca acgggataac cgacaacatg cgagaggtga tcccgctcgc
     1141 cgcaagtcga tgaccgagcg tagcgagcga atcgacgagg aagcggaaga gaaccggaag
     1201 ttacattgag cacttacgca cggatgcggg gtgtcggggt gaagccctga ccaggtgggg
     1261 aatgtctgag tgcgcgtgcg cggtcagaca ttcccacatc ctgtcccgat ttctgaggcg
     1321 ttttaacggt gaacggacgg caaaaacatg atcgagctgt tgaacgtgga gatcgcgaaa
     1381 tcgggcaaaa tcatggctga cgaggcttgc agaggagtaa gtcagtatac actccgctat
     1441 cgctacgtga cctggtcagg gcttcgcccc gacaccccaa aagggcgttg cgttgcgcgc
     1501 aacacccttg cctagaatag atcttttacg ccgatttgta agttgt
//
