LOCUS       NZ_OR237802             8401 bp    DNA     circular CON 11-SEP-2023
DEFINITION  Shigella sonnei strain CL-059 plasmid pCL059-2, complete sequence.
ACCESSION   NZ_OR237802
VERSION     NZ_OR237802.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Yassine,I., Hamze,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2023) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/11/2023 07:11:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OR237802.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="CL-059"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="pCL059-2"
                     /geo_loc_name="Lebanon"
                     /collection_date="2016"
     ARG             complement(2..288)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="35.42"
     ARG             complement(7875..8401)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="64.58"
     gene            complement(748..906)
                     /locus_tag="RI161_RS00010"
     CDS             complement(748..906)
                     /locus_tag="RI161_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            1477..1626
                     /locus_tag="RI161_RS00015"
                     /old_locus_tag="pCL059-2_00001"
     CDS             1477..1626
                     /locus_tag="RI161_RS00015"
                     /old_locus_tag="pCL059-2_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(2393..2833)
                     /locus_tag="RI161_RS00020"
                     /old_locus_tag="pCL059-2_00002"
     CDS             complement(2393..2833)
                     /locus_tag="RI161_RS00020"
                     /old_locus_tag="pCL059-2_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            3018..3440
                     /locus_tag="RI161_RS00025"
                     /old_locus_tag="pCL059-2_00003"
     CDS             3018..3440
                     /locus_tag="RI161_RS00025"
                     /old_locus_tag="pCL059-2_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3484..>3672
                     /locus_tag="RI161_RS00030"
                     /old_locus_tag="pCL059-2_00004"
                     /pseudo=""
     CDS             3484..>3672
                     /locus_tag="RI161_RS00030"
                     /old_locus_tag="pCL059-2_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(3639..4838)
                     /gene="tet(A)"
                     /locus_tag="RI161_RS00035"
                     /old_locus_tag="pCL059-2_00005"
     ARG             complement(3643..4838)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4944..5594
                     /gene="tetR(A)"
                     /locus_tag="RI161_RS00040"
                     /old_locus_tag="pCL059-2_00006"
     CDS             4944..5594
                     /gene="tetR(A)"
                     /locus_tag="RI161_RS00040"
                     /old_locus_tag="pCL059-2_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(5626..5868)
                     /locus_tag="RI161_RS00045"
                     /old_locus_tag="pCL059-2_00007"
     CDS             complement(5626..5868)
                     /locus_tag="RI161_RS00045"
                     /old_locus_tag="pCL059-2_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(6174..7010)
                     /locus_tag="RI161_RS00050"
                     /old_locus_tag="pCL059-2_00008"
     CDS             complement(6174..7010)
                     /locus_tag="RI161_RS00050"
                     /old_locus_tag="pCL059-2_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(7010..7813)
                     /gene="aph(3'')-Ib"
                     /locus_tag="RI161_RS00055"
                     /old_locus_tag="pCL059-2_00009"
     ARG             complement(7014..7813)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     ARG             complement(6178..7010)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1269..1442
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(740..1345)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caaggcatag gcttgcgtcg cgggttgata actgtcgagc gagacgggaa tgccatctgc
       61 cttgagcgcg tccagcaccg gcgcgatacg cgcgatttct gtgtcggacg aaacaggcgc
      121 ggcgtcggga ttgctggatg ccggaccgag gtcgatcaca tctgccccct cggccatcag
      181 cttacgcgcc tgcgcaatgg ctgcgtctgg cgccagatac cggcctccat cggagaaact
      241 gtccgaggtt atgttgacga tgccgaaaat gatgagcgat ttattcatgg gggcttctat
      301 aataataata atcgagcatg agtctcatac ggatgctcgg gtcgaaaggg aatccccagg
      361 cgagtaacct gtttgcggtg atccgtttca tgatgatgta tctgtagcat tttggaaata
      421 aatatatcat ctctcaataa gtagatatat ttatcttttt tgcttctttg gtgctgcaag
      481 tcaccaatac gagggaaacc tgttacggag cggtttaacc gtaatcaaca gtgactggtg
      541 tcatgttcgg aagattaatt gatagtaatc tacaaaggat cttcttgaga tccttttcaa
      601 tgaatcgtaa tctcttgctc tgtaaacgaa aaaaccgcct ggggaggcgg tttgttcgaa
      661 ggttaagtca gttggggaac tgcttaaccc ggtaacaggg ttcacgagac cgccattacc
      721 aaaactgttc tttcagtgta gccgcagtca ggcctctact tcaagacctc ttaatctatc
      781 ttctcgtgca ctcctgttac taatggctgc tgccagtggc gttttgtcgt gtctttccgg
      841 gttggactca agttgatagt taccggacaa ggcgcagcgg tcggactgaa cggggggttc
      901 gtgcatacag tccagcttgg agcgaactgc cttcacggaa ctgagtgtca ggcgtggaat
      961 gagaaaccgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     1021 gcgcacgagg gagctaccag ggggaaacgc ctgggatctt tatagtcctg tcgggtttcg
     1081 ccaccactga tttgagcgtc agattttgcg atgcttgtca ggggggcgga gcctatggaa
     1141 aaacggcttc gccgtggcct tatctcttct ttgctaagtg tcttcctggt atcttccagg
     1201 aaatttccgt ccccgtccgt aagccggtac cgctcgccgc agccgaacga ccgagcgtag
     1261 cgagtcagtg agcgaggaag cggaatatct cctgtatcgc atattctgct gacgcgccgg
     1321 tgctgccttt tttctcctgc cacatgaagc acttcactga ttttcgcaat cgtggcaaca
     1381 tagtaagcca gtatacactc cgctagcgct acatgactgg ttcagggctg cgccccgaaa
     1441 cccgctaaca acctctcagc tactggatat caccccatgc catctgaata tgatgaacaa
     1501 ctggattcga ctgatacgca gcttcccggc gaatatgacc tttttgattt aatggcgcag
     1561 gttactgaag agaacctgca tgcccctgtg gattttgggt cgcctgtcgg ccgggaagcg
     1621 ttatagcttt ttcgcttgcc cgcgtacgtg gtaatactcc ggtattacct tttatttctt
     1681 tattttcatg taattacagt ggatttttaa tacccgggta ttactcgtga aaacgaaata
     1741 ttcctttttt ttcttttaat tcatgcagtt gcgatgaaaa tcgcggctgc gttaggtgta
     1801 tgaccatttt gaggttgaaa aataatcaac catggcgggt gatttacgca ggttaagggg
     1861 agatcgtgac aaaaaaagcg ttcctcactg tatcgttcat gcctgcatga acgggttatt
     1921 cttcctgact acagaattac atcgctcctt cagttcatac atcactttta catcacttat
     1981 acatcacttt tacatcactt atacatcact tttacatcat tccttcagct catacgtcac
     2041 tttcatcctc tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt
     2101 ccggctgcgc cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt
     2161 cggatgggaa gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc
     2221 tatgcacttg tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg
     2281 gttttcgggt ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct
     2341 ctcaggcggt gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg
     2401 aaccggagcg aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag
     2461 ccagacggct aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg
     2521 cttcccgcgc catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt
     2581 tctgctgaat gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc
     2641 cctccttgtg cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt
     2701 cactggtctc gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag
     2761 ccatgtcacg atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg
     2821 tgtgccatgc cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca
     2881 tattactgtt ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa
     2941 aacctgttga cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat
     3001 tgttataaat acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag
     3061 agttaaatag cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg
     3121 atggtcatag cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc
     3181 atgataatag tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc
     3241 cgataactgt cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata
     3301 acattataca tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa
     3361 gagtgggagt gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg
     3421 gtaaaagaac tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg
     3481 aacatgcctc atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag
     3541 agccaccgac ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc
     3601 gctgcgctta tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa
     3661 gaatttctcc aaagcccgcg acgcagcgcc ggcaggcaga gcaagtagag ggcagcgcct
     3721 gcaatccatg cccacccgtt ccacgttgtt atagaagccg catagatcgc cgtgaagagg
     3781 aggggtccga cgatcgaggt caggctggtg agcgccgcca gtgagccttg cagctgcccc
     3841 tgacgttcct catccacctg cctggacaac attgcttgca gcgccggcat tccgatgcca
     3901 cccgaagcaa gcaggaccat gatcgggaac gccatccatc cccgtgtcgc gaaggcaagc
     3961 aggatgtagc ctgtgccgtc ggcaatcatt ccgagcatga gtgcccgcct ttcgccgagc
     4021 cgggcggcta cagggccggt gatcattgcc tgggcgagtg aatgcagaat gccaaatgcg
     4081 gcaagcgaaa tgccgatcgt ggtcgcgtcc cagtgaaagc gatcctcgcc gaaaatgacc
     4141 caaagcgcgg ccggcacctg tccgacaagt tgcatgatga agaagaccgc catcagggcg
     4201 gcgacgacgg tcatgccccg ggcccaccgg aacgaagcga gcgggttgag agcctcccgg
     4261 cgtaacggcc ggcgttcgcc tttgtgcgac tccggcaaaa ggaaacagcc cgtcaggaaa
     4321 ttgaggccgt tcaaggctgc cgcggcgaag aacggagcgt ggggggagaa accgcccatc
     4381 agcccaccga gcacaggtcc cgcgaccatc ccgaacccga aacaggcgct catgaagccg
     4441 aagtgccgcg cgcgctcatc gccatcagtg atatcggcaa tataagcgcc ggctaccgcc
     4501 ccagtcgccc cggtgatgcc ggccacgatc cgcccgatat agagaaccca aaggaaaggc
     4561 gccgtcgcca tgatggcgta gtcgacagca gcgccggcca gcgagacgag caagaccggc
     4621 cgccgcccga aacgatccga cagcgcgccc agcacaggtg cgcaggcaaa ttgcatcaac
     4681 gcatacagcg ccagcagaat gccatagtgg gcggtgacgt cgttcgagtg aaccagatcg
     4741 cgcaggaggc ccggcagcac cggcataatc aggccgatgc cgacagcgtc gagcgcgaca
     4801 gtgctcagaa ttacgatcag gggtctgttg ggtttcacgt ctggcctccg gaccagcctc
     4861 cgctggtccg attgaacgcg cggattcttt atcactgata agttggtgga catattatgt
     4921 ttatcagtga taaagtgtca agcatgacaa agttgcagcc gaatacagtg atccgtgccg
     4981 ccctggacct gttgaacgag gtcggcgtag acggtctgac gacacgcaaa ctggcggaac
     5041 ggttgggggt tcagcagccg gcgctttact ggcacttcag gaacaagcgg gcgctgctcg
     5101 acgcactggc cgaagccatg ctggcggaga atcatacgca ttcggtgccg agagccgacg
     5161 acgactggcg ctcatttctg atcgggaatg cccgcagctt caggcaggcg ctgctcgcct
     5221 accgcgatgg cgcgcgcatc catgccggca cgcgaccggg cgcaccgcag atggaaacgg
     5281 ccgacgcgca gcttcgcttc ctctgcgagg cgggtttttc ggccggggac gccgtcaatg
     5341 cgctgatgac aatcagctac ttcactgttg gggccgtgct tgaggagcag gccggcgaca
     5401 gcgatgccgg cgagcgcggc ggcaccgttg aacaggctcc gctctcgccg ctgttgcggg
     5461 ccgcgataga cgccttcgac gaagccggtc cggacgcagc gttcgagcag ggactcgcgg
     5521 tgattgtcga tggattggcg aaaaggaggc tcgttgtcag gaacgttgaa ggaccgagaa
     5581 agggtgacga ttgatcagga ccgctgccgg agcgcaaccc actcactaca gcagagccat
     5641 gtagacaaca tcccctcccc ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg
     5701 ctttttcatg ccctgcccta gcgtccaagc ctcacggccg cgctcggcct ctctggcggc
     5761 cttctggcgc tcctgctgcg gcgtccgctc gtgggccgtg gcgcgggtcc gcgcgccggc
     5821 ctcgtgcgcc tggcgctcgc gggcgaggtc cagggcggcc gtcttcacgt tctgccttgc
     5881 gcagatgaga tagatctagc gtggactcaa ggctctcgcg aatggctcgc gttggaaact
     5941 ttcattgaca cttgaggggc accgcaggga aattctcgtc cttgcgagaa ccggctatgt
     6001 cgtgctgcgc atcgagcctg cgcccttggc ttgtctcgcc cctctccgcg tcgctacggg
     6061 gcttccaggg gtcgtttgcg ggaaggggcg gaatcctacg ctaaggcttt ggccagcgat
     6121 attctccggt gagattgatg tgttcccagg ggataggaga agtcgcttga tatctagtat
     6181 gacgtctgtc gcacctgctt gatcgcggcc gcgatagcta gatcgcgttg ctcctcttct
     6241 ccatccgcgt tccaagctgc ggaaaggcac ccataagcgt acgcctggtc gagcaggcga
     6301 cgcggatcga cgtccagcgc acgagagaat gcgtccgcca tctgtgcaat gcgtctagga
     6361 tcgagacaaa ggtcgtctct gtcagccgga tcgtagaaca tattggcggc gccaaagccc
     6421 acttcaccga ccagaccgac gggatctatc accagccagc cgcgactgga gaacatgatg
     6481 ttttcatgat gcagatcgcc atgtagccca cgcagttccg aggcattgct catcatttga
     6541 tcggctataa tcgccgcgtg gacgtagtca gtttgacaac ctgcgttttg atcatcgcgc
     6601 gcccgctgaa acaaagctgc aaagcgatcc cggatcggga gaagggcaga aggcaggggt
     6661 tcctcagatg cggcatacag cttcgccatt agttccgctg caatttcggt cgcctggtag
     6721 tcgccgtgct cggcaacgat gtgagagagc attcgctccc cggcatattc gagcaacatc
     6781 agattgttct cacgaccgag caaccggact gctcccctcc cattgcgcca taccagatag
     6841 tcggccccgc gcagttcatc agcaatgtct tctataggtt tcaatccctt gacgattgca
     6901 ggagtcccgt ctggcaatga aactttccaa acgaggctgg aaaaggtgtc cgcaatgaga
     6961 acaggttgcg aaacgtgcca atgagcagga aaaacaggcg gcatgaacat caaccccaag
     7021 tcagagggtc caatcgcaga tagaaggcaa ggcgttcgcg gtcgggggct tcgatcccca
     7081 atacattgaa taggacagcg aaggcgcgct ctgcttcatc tggcgctgcc cagttctctt
     7141 cggcgttagc aatcatgagt gccaaatcgg catagcgatc tgctgttccg agccgcccaa
     7201 ggtcgatcag acccgtgcat tgaagagttt tagggtccac catgaagttc ggcatgcagg
     7261 gatcaccatg gcaaacaacc atatcggtgc gctcttggtc gagccgcacc ggtagctctc
     7321 gttcgacacg agccaaaaga tcgagctgcg gcgtactctt gtcctcgtcc ggtaagaagt
     7381 cgggattgac ggcattgcgg gacaccacat caacggcgcg tccgaacatt cgcgacagcc
     7441 tgcgctcaaa ctgacattga tcaaccgata ggctgtgaac agcgccaagt tgctgcccca
     7501 ttgacggcca cgctttgagc aaatccgctc cagacagatc agccgccggt actcccggaa
     7561 ttgccgttat caccaagcat gcaccctcct gttcctcctg ccagttgatg acctcggggc
     7621 aagccacacc tcgacctttg agccaaatga ggcggtcacg ctctccagcg agctcaccgc
     7681 ggcgggaagc aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga aaaacaaaat
     7741 caccagattc tccgcctctg acaggcaacc agtcagaatg cgattcacca aaaaaaatat
     7801 tagttcgatt caatggaggt tccttcagtt ttctgatgaa gcgcgaatat agagaaatat
     7861 cccgaatgtg cagttaacga attcttgcgg tttctttcag cgccgccaat accgccagcc
     7921 cgtcgcgcaa ggggcgcggc tcgtgtgtgc ggatgaagtc agctccacct gcggcggcgg
     7981 caagctctgc agcgagtgtc gcggccccga catcccccgg accacggcct gtgagcgcgc
     8041 gcagaaagga tttgcgcgaa acagacagaa gcaccggcaa atcgaagcgc agccgcaatt
     8101 catcgaaccg cgccagcacc gagagcgagg tttcgggagc agcccccaga aaaaacccca
     8161 tgccgggatc aaggacaagg cggttgcgtt tgataccggc acccgtcagc gccgcgatgc
     8221 gcgcgtcaaa gaacgccgca atgtgatcca tgatgtcgcc agcgggtgcc tcgcgccgat
     8281 ctgcctgccc gtcttgcacc gaatgcataa cgacgagttt ggcagatgat ttcgccaatt
     8341 gcggatagaa cgcagcgtct ggaaaaccgc gaatatcatt gagataggcc acaccacgcg
     8401 a
//
