LOCUS       NZ_OR237800             8401 bp    DNA     circular CON 11-SEP-2023
DEFINITION  Shigella sonnei strain CL-053 plasmid pCL053-2, complete sequence.
ACCESSION   NZ_OR237800
VERSION     NZ_OR237800.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Yassine,I., Hamze,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2023) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/11/2023 07:10:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OR237800.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="CL-053"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="pCL053-2"
                     /geo_loc_name="Lebanon"
                     /collection_date="2015"
     ARG             4..959
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="75.0"
     ARG             8162..8400
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="98.75"
                     /coverage="18.87"
     gene            complement(<926..1114)
                     /locus_tag="RI156_RS00010"
                     /old_locus_tag="pCL053-2_00002"
                     /pseudo=""
     CDS             complement(<926..1114)
                     /locus_tag="RI156_RS00010"
                     /old_locus_tag="pCL053-2_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(1158..1580)
                     /locus_tag="RI156_RS00015"
                     /old_locus_tag="pCL053-2_00003"
     CDS             complement(1158..1580)
                     /locus_tag="RI156_RS00015"
                     /old_locus_tag="pCL053-2_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            1765..2205
                     /locus_tag="RI156_RS00020"
                     /old_locus_tag="pCL053-2_00004"
     CDS             1765..2205
                     /locus_tag="RI156_RS00020"
                     /old_locus_tag="pCL053-2_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(2972..3121)
                     /locus_tag="RI156_RS00025"
                     /old_locus_tag="pCL053-2_00005"
     CDS             complement(2972..3121)
                     /locus_tag="RI156_RS00025"
                     /old_locus_tag="pCL053-2_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            3692..3850
                     /locus_tag="RI156_RS00030"
     CDS             3692..3850
                     /locus_tag="RI156_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            4310..5125
                     /gene="sul2"
                     /locus_tag="RI156_RS00035"
                     /old_locus_tag="pCL053-2_00006"
     ARG             4311..5122
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5186..5989
                     /gene="aph(3'')-Ib"
                     /locus_tag="RI156_RS00040"
                     /old_locus_tag="pCL053-2_00007"
     ARG             5187..5986
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            5989..6825
                     /locus_tag="RI156_RS00045"
                     /old_locus_tag="pCL053-2_00008"
     CDS             5989..6825
                     /locus_tag="RI156_RS00045"
                     /old_locus_tag="pCL053-2_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            7131..7373
                     /locus_tag="RI156_RS00050"
                     /old_locus_tag="pCL053-2_00009"
     CDS             7131..7373
                     /locus_tag="RI156_RS00050"
                     /old_locus_tag="pCL053-2_00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(7405..8055)
                     /gene="tetR(A)"
                     /locus_tag="RI156_RS00055"
                     /old_locus_tag="pCL053-2_00010"
     CDS             complement(7405..8055)
                     /gene="tetR(A)"
                     /locus_tag="RI156_RS00055"
                     /old_locus_tag="pCL053-2_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     ARG             5990..6822
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(3157..3330)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          3254..3859
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccggcgctgc tgtcgactac gccatcatgg cgacggcgcc tttcctttgg gttctctata
       61 tcgggcggat cgtggccggc atcaccgggg cgactggggc ggtagccggc gcttatattg
      121 ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg cttcatgagc gcctgtttcg
      181 ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat gggcggtttc tccccccacg
      241 ctccgttctt cgccgcggca gccttgaacg gcctcaattt cctgacgggc tgtttccttt
      301 tgccggagtc gcacaaaggc gaacgccggc cgttacgccg ggaggctctc aacccgctcg
      361 cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc cctgatggcg gtcttcttca
      421 tcatgcaact tgtcggacag gtgccggccg cgctttgggt cattttcggc gaggatcgct
      481 ttcactggga cgcgaccacg atcggcattt cgcttgccgc atttggcatt ctgcattcac
      541 tcgcccaggc aatgatcacc ggccctgtag ccgcccggct cggcgaaagg cgggcactca
      601 tgctcggaat gattgccgac ggcacaggct acatcctgct tgccttcgcg acacggggat
      661 ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg catcggaatg ccggcgctgc
      721 aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg gcagctgcaa ggctcactgg
      781 cggcgctcac cagcctgacc tcgatcgtcg gacccctcct cttcacggcg atctatgcgg
      841 cttctataac aacgtggaac gggtgggcat ggattgcagg cgctgccctc tacttgctct
      901 gcctgccggc gctgcgtcgc gggctttgga gaaattcttc aaattcccgt tgcacatagc
      961 ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa
     1021 cgatctgata gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat
     1081 cccggctggc cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt
     1141 tacatacagt ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt
     1201 gctagacatt gccagccagt gcccgtcact cccactctta cattgttcgc agtatctagg
     1261 tacatgttat tgcgtgcacc ttcatgatgt ataatgttat taatgacagc agaaagatcg
     1321 acagaattaa tattcttcgt acctgggaca gttatcgggt gtcggccagt gatgagacca
     1381 aaggatgcag tataagatga accactacta ttatcatggc caccattacc accaccgtta
     1441 aaactacctc ccgaacttcc agagccgcta tgaccatcat tgtaggggtc atttggtccc
     1501 atgtgaccaa aaccaccgac agtaccgcta tttaactctg tactcataaa attatcctct
     1561 ttgactttta aaaacagcaa aaactgtatt tataacaatg tgtttatata cagtattcga
     1621 aatactacgc ccgtcaaaac cacaccgtca acaggttttt tgtgtatttt tcacgcctca
     1681 cgggcagcct cagacctttt gtgttaaaac agtaatatgt tctgctgtta caatacggta
     1741 cacacaggag acactgtcac ttttatggca tggcacacag ttcaagagac actgaaatta
     1801 acaggaaagt cgcgctcgca attatatcgt gacatggcta aaggccttgt cagctatcgc
     1861 acaggaaaaa atgatcgccg ggagttcgag accagtgaac tgattcgggc ctatggcgag
     1921 ctgcgcactt ctgagacacc aaaaaggcac aaggagggac actccaaatc tgtcgttagc
     1981 caggcggaaa atgctcagct agacgtcatt cagcagaaac tggacgctct gcagcagact
     2041 gttaccctaa tgcttgaaga caagatggcg cgggaagcag aaaaccgcct gcgaaatgag
     2101 gagagggaac aactacaggc cgaaattagc cgtctggctg acgcgctggc acaggaaaaa
     2161 aagcggggat tctggtcgcg gctgtttcgc tccggttcag agtaacagtg caggtgactc
     2221 tgatttacca taacatctgt tatcagcacc gcctgagagg ccctcagggc gtttctgacg
     2281 ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg ccagaaacgc gcaggctctg
     2341 tttttacggc atgcatgagt atgcgcacaa gtgcatagcg cggaaattca caccattcag
     2401 ccggggtaag tcctgtttcc ggcgttcttc ccatccgaaa acggtgaacg gggacgcaat
     2461 ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa acccgctgtc agcacgttca
     2521 ggaagggggt gatgtacaaa tggcacagag gatgaaagtg acgtatgagc tgaaggaatg
     2581 atgtaaaagt gatgtataag tgatgtaaaa gtgatgtata agtgatgtaa aagtgatgta
     2641 tgaactgaag gagcgatgta attctgtagt caggaagaat aacccgttca tgcaggcatg
     2701 aacgatacag tgaggaacgc tttttttgtc acgatctccc cttaacctgc gtaaatcacc
     2761 cgccatggtt gattattttt caacctcaaa atggtcatac acctaacgca gccgcgattt
     2821 tcatcgcaac tgcatgaatt aaaagaaaaa aaaggaatat ttcgttttca cgagtaatac
     2881 ccgggtatta aaaatccact gtaattacat gaaaataaag aaataaaagg taataccgga
     2941 gtattaccac gtacgcgggc aagcgaaaaa gctataacgc ttcccggccg acaggcgacc
     3001 caaaatccac aggggcatgc aggttctctt cagtaacctg cgccattaaa tcaaaaaggt
     3061 catattcgcc gggaagctgc gtatcagtcg aatccagttg ttcatcatat tcagatggca
     3121 tggggtgata tccagtagct gagaggttgt tagcgggttt cggggcgcag ccctgaacca
     3181 gtcatgtagc gctagcggag tgtatactgg cttactatgt tgccacgatt gcgaaaatca
     3241 gtgaagtgct tcatgtggca ggagaaaaaa ggcagcaccg gcgcgtcagc agaatatgcg
     3301 atacaggaga tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcggctgcg
     3361 gcgagcggta ccggcttacg gacggggacg gaaatttcct ggaagatacc aggaagacac
     3421 ttagcaaaga agagataagg ccacggcgaa gccgtttttc cataggctcc gcccccctga
     3481 caagcatcgc aaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
     3541 atcccaggcg tttccccctg gtagctccct cgtgcgctct cctgttcctg cctttcggtt
     3601 taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga cactcagttc
     3661 cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     3721 ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg acaaaacgcc
     3781 actggcagca gccattagta acaggagtgc acgagaagat agattaagag gtcttgaagt
     3841 agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc tcgtgaaccc
     3901 tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg cctccccagg
     3961 cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct caagaagatc
     4021 ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt tgattacggt
     4081 taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa agaagcaaaa
     4141 aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg ctacagatac
     4201 atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc tttcgacccg
     4261 agcatccgta tgagactcat gctcgattat tattattata gaagccccca tgaataaatc
     4321 gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg gaggccggta
     4381 tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg gggcagatgt
     4441 gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt ccgacacaga
     4501 aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc ccgtctcgct
     4561 cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg cctatctcaa
     4621 tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat catctgccaa
     4681 actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg cacccgctgg
     4741 cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc tgacgggtgc
     4801 cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc tgggggctgc
     4861 tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc gcttcgattt
     4921 gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag gccgtggtcc
     4981 gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag gtggagctga
     5041 cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat tggcggcgct
     5101 gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat attcgcgctt
     5161 catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg gtgaatcgca
     5221 ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc gacgtggtga
     5281 cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg ctggagagcg
     5341 tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca tcaactggca
     5401 ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac cggcggctga
     5461 tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac ttggcgctgt
     5521 tcacagccta tcggttgatc aatgtcagtt tgagcgcagg ctgtcgcgaa tgttcggacg
     5581 cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg acgaggacaa
     5641 gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg tgcggctcga
     5701 ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga acttcatggt
     5761 ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg gaacagcaga
     5821 tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg cagcgccaga
     5881 tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag cccccgaccg
     5941 cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat gttcatgccg
     6001 cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga caccttttcc
     6061 agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa gggattgaaa
     6121 cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg gcgcaatggg
     6181 aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga atatgccggg
     6241 gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac cgaaattgca
     6301 gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc tgcccttctc
     6361 ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca aaacgcaggt
     6421 tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag caatgcctcg
     6481 gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc cagtcgcggc
     6541 tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc cgccaatatg
     6601 ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat tgcacagatg
     6661 gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga ccaggcgtac
     6721 gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca acgcgatcta
     6781 gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc aagcgacttc
     6841 tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa agccttagcg
     6901 taggattccg ccccttcccg caaacgaccc ctggaagccc cgtagcgacg cggagagggg
     6961 cgagacaagc caagggcgca ggctcgatgc gcagcacgac atagccggtt ctcgcaagga
     7021 cgagaatttc cctgcggtgc ccctcaagtg tcaatgaaag tttccaacgc gagccattcg
     7081 cgagagcctt gagtccacgc tagatctatc tcatctgcgc aaggcagaac gtgaagacgg
     7141 ccgccctgga cctcgcccgc gagcgccagg cgcacgaggc cggcgcgcgg acccgcgcca
     7201 cggcccacga gcggacgccg cagcaggagc gccagaaggc cgccagagag gccgagcgcg
     7261 gccgtgaggc ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc tgctacgggc
     7321 gtctgacgcg gtggaaaggg ggaggggatg ttgtctacat ggctctgctg tagtgagtgg
     7381 gttgcgctcc ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt caacgttcct
     7441 gacaacgagc ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc
     7501 tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg
     7561 agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag
     7621 cacggcccca acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga
     7681 aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc
     7741 cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa
     7801 gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg
     7861 cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct
     7921 gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt
     7981 cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg
     8041 ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta
     8101 tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac
     8161 gtgaaaccca acagacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc
     8221 atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac
     8281 gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tgatgcaatt tgcctgcgca
     8341 cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc cggtcttgct cgtctcgctg
     8401 g
//
