LOCUS       NZ_OR237796             8401 bp    DNA     circular CON 11-SEP-2023
DEFINITION  Shigella sonnei strain CL-022 plasmid pCL022-2, complete sequence.
ACCESSION   NZ_OR237796
VERSION     NZ_OR237796.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Yassine,I., Hamze,M. and Weill,F.-X.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2023) Enteric Bacterial Pathogens Unit, Institut
            Pasteur, 28 rue du Dr Roux, Paris 75724, France
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Sequencing Technology             :: MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/11/2023 07:10:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OR237796.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="CL-022"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="pCL022-2"
                     /geo_loc_name="Lebanon"
                     /collection_date="2012"
     gene            join(8362..8401,1..401)
                     /locus_tag="RI154_RS00005"
                     /old_locus_tag="pCL022-2_00001"
     CDS             join(8362..8401,1..401)
                     /locus_tag="RI154_RS00005"
                     /old_locus_tag="pCL022-2_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(1168..1317)
                     /locus_tag="RI154_RS00010"
                     /old_locus_tag="pCL022-2_00002"
     CDS             complement(1168..1317)
                     /locus_tag="RI154_RS00010"
                     /old_locus_tag="pCL022-2_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1888..2046
                     /locus_tag="RI154_RS00015"
     CDS             1888..2046
                     /locus_tag="RI154_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            2506..3321
                     /gene="sul2"
                     /locus_tag="RI154_RS00020"
                     /old_locus_tag="pCL022-2_00003"
     ARG             2507..3318
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3382..4185
                     /gene="aph(3'')-Ib"
                     /locus_tag="RI154_RS00025"
                     /old_locus_tag="pCL022-2_00004"
     ARG             3383..4182
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            4185..5021
                     /locus_tag="RI154_RS00030"
                     /old_locus_tag="pCL022-2_00005"
     CDS             4185..5021
                     /locus_tag="RI154_RS00030"
                     /old_locus_tag="pCL022-2_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            5327..5569
                     /locus_tag="RI154_RS00035"
                     /old_locus_tag="pCL022-2_00006"
     CDS             5327..5569
                     /locus_tag="RI154_RS00035"
                     /old_locus_tag="pCL022-2_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(5601..6251)
                     /gene="tetR(A)"
                     /locus_tag="RI154_RS00040"
                     /old_locus_tag="pCL022-2_00007"
     CDS             complement(5601..6251)
                     /gene="tetR(A)"
                     /locus_tag="RI154_RS00040"
                     /old_locus_tag="pCL022-2_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            6357..7556
                     /gene="tet(A)"
                     /locus_tag="RI154_RS00045"
                     /old_locus_tag="pCL022-2_00008"
     ARG             6358..7553
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<7523..7711)
                     /locus_tag="RI154_RS00050"
                     /old_locus_tag="pCL022-2_00009"
                     /pseudo=""
     CDS             complement(<7523..7711)
                     /locus_tag="RI154_RS00050"
                     /old_locus_tag="pCL022-2_00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(7755..8177)
                     /locus_tag="RI154_RS00055"
                     /old_locus_tag="pCL022-2_00010"
     CDS             complement(7755..8177)
                     /locus_tag="RI154_RS00055"
                     /old_locus_tag="pCL022-2_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             4186..5018
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1353..1526)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          1450..2055
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gaaagtcgcg ctcgcaatta tatcgtgaca tggctaaagg ccttgtcagc tatcgcacag
       61 gaaaaaatga tcgccgggag ttcgagacca gtgaactgat tcgggcctat ggcgagctgc
      121 gcacttctga gacaccaaaa aggcacaagg agggacactc caaatctgtc gttagccagg
      181 cggaaaatgc tcagctagac gtcattcagc agaaactgga cgctctgcag cagactgtta
      241 ccctaatgct tgaagacaag atggcgcggg aagcagaaaa ccgcctgcga aatgaggaga
      301 gggaacaact acaggccgaa attagccgtc tggctgacgc gctggcacag gaaaaaaagc
      361 ggggattctg gtcgcggctg tttcgctccg gttcagagta acagtgcagg tgactctgat
      421 ttaccataac atctgttatc agcaccgcct gagaggccct cagggcgttt ctgacgggaa
      481 accggtaaaa caggcaacaa accacccgaa aacccgccag aaacgcgcag gctctgtttt
      541 tacggcatgc atgagtatgc gcacaagtgc atagcgcgga aattcacacc attcagccgg
      601 ggtaagtcct gtttccggcg ttcttcccat ccgaaaacgg tgaacgggga cgcaatttat
      661 tgcgtggccg tgaagcgttg ccggcgcagc cggaaaaccc gctgtcagca cgttcaggaa
      721 gggggtgatg tacaaatggc acagaggatg aaagtgacgt atgagctgaa ggaatgatgt
      781 aaaagtgatg tataagtgat gtaaaagtga tgtataagtg atgtaaaagt gatgtatgaa
      841 ctgaaggagc gatgtaattc tgtagtcagg aagaataacc cgttcatgca ggcatgaacg
      901 atacagtgag gaacgctttt tttgtcacga tctcccctta acctgcgtaa atcacccgcc
      961 atggttgatt atttttcaac ctcaaaatgg tcatacacct aacgcagccg cgattttcat
     1021 cgcaactgca tgaattaaaa gaaaaaaaag gaatatttcg ttttcacgag taatacccgg
     1081 gtattaaaaa tccactgtaa ttacatgaaa ataaagaaat aaaaggtaat accggagtat
     1141 taccacgtac gcgggcaagc gaaaaagcta taacgcttcc cggccgacag gcgacccaaa
     1201 atccacaggg gcatgcaggt tctcttcagt aacctgcgcc attaaatcaa aaaggtcata
     1261 ttcgccggga agctgcgtat cagtcgaatc cagttgttca tcatattcag atggcatggg
     1321 gtgatatcca gtagctgaga ggttgttagc gggtttcggg gcgcagccct gaaccagtca
     1381 tgtagcgcta gcggagtgta tactggctta ctatgttgcc acgattgcga aaatcagtga
     1441 agtgcttcat gtggcaggag aaaaaaggca gcaccggcgc gtcagcagaa tatgcgatac
     1501 aggagatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga
     1561 gcggtaccgg cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag
     1621 caaagaagag ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag
     1681 catcgcaaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc
     1741 caggcgtttc cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     1801 ggtgtcattc cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg
     1861 aaggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
     1921 gccttgtccg gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg
     1981 gcagcagcca ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag
     2041 gcctgactgc ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt
     2101 accgggttaa gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt
     2161 tttttcgttt acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt
     2221 gtagattact atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa
     2281 ccgctccgta acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga
     2341 taaatatatc tacttattga gagatgatat atttatttcc aaaatgctac agatacatca
     2401 tcatgaaacg gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca
     2461 tccgtatgag actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc
     2521 atcattttcg gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg
     2581 gcgccagacg cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc
     2641 gacctcggtc cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc
     2701 gcgcgtatcg cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac
     2761 agttatcaac ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat
     2821 attcgcggtt ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc
     2881 gtcgttatgc attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac
     2941 atcatggatc acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt
     3001 atcaaacgca accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc
     3061 gaaacctcgc tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg
     3121 gtgcttctgt ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg
     3181 gatgtcgggg ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc
     3241 atccgcacac acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa
     3301 gaaaccgcaa gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc
     3361 agaaaactga aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct
     3421 gactggttgc ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg
     3481 catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac
     3541 cgcctcattt ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag
     3601 gaacaggagg gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg
     3661 tctggagcgg atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac
     3721 agcctatcgg ttgatcaatg tcagtttgag cgcaggctgt cgcgaatgtt cggacgcgcc
     3781 gttgatgtgg tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt
     3841 acgccgcagc tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa
     3901 gagcgcaccg atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac
     3961 cctaaaactc ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc
     4021 tatgccgatt tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa
     4081 gcagagcgcg ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa
     4141 cgccttgcct tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg
     4201 tttttcctgc tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc
     4261 tcgtttggaa agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta
     4321 tagaagacat tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg
     4381 gagcagtccg gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc
     4441 gaatgctctc tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg
     4501 aactaatggc gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga
     4561 tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc
     4621 aaactgacta cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac
     4681 tgcgtgggct acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc
     4741 tggtgataga tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct
     4801 acgatccggc tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg
     4861 acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt
     4921 atgggtgcct ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta
     4981 tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct
     5041 atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg
     5101 attccgcccc ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag
     5161 acaagccaag ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag
     5221 aatttccctg cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag
     5281 agccttgagt ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc
     5341 cctggacctc gcccgcgagc gccaggcgca cgaggccggc gcgcggaccc gcgccacggc
     5401 ccacgagcgg acgccgcagc aggagcgcca gaaggccgcc agagaggccg agcgcggccg
     5461 tgaggcttgg acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct
     5521 gacgcggtgg aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg
     5581 cgctccggca gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca
     5641 acgagcctcc ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg
     5701 tccggaccgg cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc
     5761 tgttcaacgg tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg
     5821 gccccaacag tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa
     5881 cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt
     5941 cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg
     6001 cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta
     6061 tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag
     6121 tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga
     6181 ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc
     6241 aactttgtca tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag
     6301 tgataaagaa tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga
     6361 aacccaacag acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg
     6421 gcctgattat gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca
     6481 ccgcccacta tggcattctg ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg
     6541 tgctgggcgc gctgtcggat cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg
     6601 gcgctgctgt cgactacgcc atcatggcga cggcgccttt cctttgggtt ctctatatcg
     6661 ggcggatcgt ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg
     6721 atatcactga tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt
     6781 tcgggatggt cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc
     6841 cgttcttcgc cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc
     6901 cggagtcgca caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt
     6961 cgttccggtg ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca
     7021 tgcaacttgt cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc
     7081 actgggacgc gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg
     7141 cccaggcaat gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc
     7201 tcggaatgat tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga
     7261 tggcgttccc gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag
     7321 caatgttgtc caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg
     7381 cgctcaccag cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt
     7441 ctataacaac gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc
     7501 tgccggcgct gcgtcgcggg ctttggagaa attcttcaaa ttcccgttgc acatagcccg
     7561 gcaattcctt tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga
     7621 tctgatagag aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc
     7681 ggctggccgt cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac
     7741 atacagtcta tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct
     7801 agacattgcc agccagtgcc cgtcactccc actcttacat tgttcgcagt atctaggtac
     7861 atgttattgc gtgcaccttc atgatgtata atgttattaa tgacagcaga aagatcgaca
     7921 gaattaatat tcttcgtacc tgggacagtt atcgggtgtc ggccagtgat gagaccaaag
     7981 gatgcagtat aagatgaacc actactatta tcatggccac cattaccacc accgttaaaa
     8041 ctacctcccg aacttccaga gccgctatga ccatcattgt aggggtcatt tggtcccatg
     8101 tgaccaaaac caccgacagt accgctattt aactctgtac tcataaaatt atcctctttg
     8161 acttttaaaa acagcaaaaa ctgtatttat aacaatgtgt ttatatacag tattcgaaat
     8221 actacgcccg tcaaaaccac accgtcaaca ggttttttgt gtatttttca cgcctcacgg
     8281 gcagcctcag accttttgtg ttaaaacagt aatatgttct gctgttacaa tacggtacac
     8341 acaggagaca ctgtcacttt tatggcatgg cacacagttc aagagacact gaaattaaca
     8401 g
//
