LOCUS       NZ_OQ821118            12710 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Escherichia coli strain 24A16CRGN002 plasmid p24A16002_B_KPC,
            complete sequence.
ACCESSION   NZ_OQ821118
VERSION     NZ_OQ821118.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   Lerminiaux,N., Mataseje,L., Fakharuddin,K., Mitchell,R.,
            Al-Rawahi,G., Davis,I., Ellis,C., Embree,J., Hota,S., Kibsey,P.,
            Leis,J.A., McGreer,A., Minion,J., Musto,S., Rajda,E., Smith,S.W.,
            Srigley,J., Suh,K.N., Thampi,N., Tomlinson,J., Wong,T., Katz,K. and
            Mulvey,M.
  TITLE     Plasmid genomic epidemiology of KPC carbapenemase-producing
            Enterobacterales in Canada, 2010-2021
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12710)
  AUTHORS   Lerminiaux,N., Mataseje,L., Fakharuddin,K., Mitchell,R.,
            Al-Rawahi,G., Davis,I., Ellis,C., Embree,J., Hota,S., Kibsey,P.,
            Leis,J.A., McGreer,A., Minion,J., Musto,S., Rajda,E., Smith,S.W.,
            Srigley,J., Suh,K.N., Thampi,N., Tomlinson,J., Wong,T., Katz,K. and
            Mulvey,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2023) Biology, Public Health Agency of Canada,
            1015 Arlington Street, Winnipeg, MB R3E3P6, Canada
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 10:22:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9
            CDSs (total)                      :: 9
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OQ821118.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="24A16CRGN002"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p24A16002_B_KPC"
                     /geo_loc_name="Canada"
                     /collected_by="Canadian Nosocomial Infection Surveillance
                     Program"
     gene            join(11689..12710,1..4)
                     /gene="istA"
                     /locus_tag="SLH58_RS00005"
     CDS             join(11689..12710,1..4)
                     /gene="istA"
                     /locus_tag="SLH58_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490293.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_004199214.1"
                     /translation="MLTQEQTVEIRVLARQGHSIRHIARTLGVSRNTVRRYLRDPSVAR
                     YQPREPRPTKLGPFESYLRQRVEQAHPIWLPATVLNREIRAQGYGGGLSLLRAFLATLK
                     PARREAGPAVRFETEPGRQLQADFVVLRRARSPMSAFVATLGYSRMTFVTFVPDESFES
                     VRDSLLLAFDYLGGVPREVLFDNMKTVVLERDAYGDGKHRFHPGLLQLADDLGFRIRLC
                     RPYRAQTKGKVERFNRYFRESFYNPLLTRMKGTGLLLDCAAANRRVRDWLADEANVRVH
                     ATLNERPIDRWRQEREHLQPLPSRVRRDEAPLLDNSLRPVPLESLQHPLSVYDAIGEAC
                     R"
                     /name="istA"
     gene            1..780
                     /gene="istB"
                     /locus_tag="SLH58_RS00010"
                     /old_locus_tag="CDKHEDKG_00001"
     CDS             1..780
                     /gene="istB"
                     /locus_tag="SLH58_RS00010"
                     /old_locus_tag="CDKHEDKG_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISKpn7 family helper ATPase
                     IstB"
                     /protein_id="WP_004152394.1"
                     /translation="MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHL
                     LRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVERAENV
                     VLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILG
                     PRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMILTSNLPFSQWADAFAGDTTLTAA
                     MLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE"
                     /name="istB"
     gene            1167..2048
                     /locus_tag="SLH58_RS00015"
                     /old_locus_tag="CDKHEDKG_00002"
     CDS             1167..2048
                     /locus_tag="SLH58_RS00015"
                     /old_locus_tag="CDKHEDKG_00002"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /protein_id="WP_004152396.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKYSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-3"
     gene            complement(2298..3617)
                     /locus_tag="SLH58_RS00020"
                     /old_locus_tag="CDKHEDKG_00003"
     CDS             complement(2298..3617)
                     /locus_tag="SLH58_RS00020"
                     /old_locus_tag="CDKHEDKG_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /protein_id="WP_004152397.1"
                     /translation="MAASYLPYRPDQSYLLPPSLGEWLPEGHLAYFISETVDTLDLSAF
                     HARYAGGGPGNQPFHPAMMVKVLIYGYASGVFSSRKLARKLYEDVALRVLAAGNFPAHR
                     TLSDFRALHLTELENLFVQVVQLARECGLVKLGTIAVDGTKVKANASRHKAMSYKRMKP
                     AEDELHCEIKALLDRAKATDDQERNEPELDIPAEISRREKRLEAIQAAKARLEARQREA
                     DQARGRSEDDGRRPRHPDGSDKGGGSYKREFGVPDDRDQESFTDPDSRIMKHAGGGSEQ
                     SYNGYTAVDAEHQIIVAAELTNCAADSQALLGMLAAVQANTGEMPAQTLADAGFRSEAV
                     LAKVADHHGDVIVALGREGREDAKVNAKTHPHTAAIAAKLKTEQGDAAYRRRKSIVEAP
                     NGWIKAVMGLRQFSMRGLDKVQAEWKLVCMALNLRRMAYL"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            4100..4744
                     /locus_tag="SLH58_RS00025"
                     /old_locus_tag="CDKHEDKG_00004"
     CDS             4100..4744
                     /locus_tag="SLH58_RS00025"
                     /old_locus_tag="CDKHEDKG_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(5246..5482)
                     /locus_tag="SLH58_RS00030"
                     /old_locus_tag="CDKHEDKG_00005"
     CDS             complement(5246..5482)
                     /locus_tag="SLH58_RS00030"
                     /old_locus_tag="CDKHEDKG_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            <6550..6720
                     /locus_tag="SLH58_RS00035"
                     /old_locus_tag="CDKHEDKG_00006"
                     /pseudo=""
     CDS             <6550..6720
                     /locus_tag="SLH58_RS00035"
                     /old_locus_tag="CDKHEDKG_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017893000.1"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6728..8443)
                     /gene="tnpR"
                     /locus_tag="SLH58_RS00040"
                     /old_locus_tag="CDKHEDKG_00007"
     CDS             complement(6728..8443)
                     /gene="tnpR"
                     /locus_tag="SLH58_RS00040"
                     /old_locus_tag="CDKHEDKG_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family resolvase TnpR"
                     /protein_id="WP_004152391.1"
                     /translation="MKTGQTTASSSTPVGFPDADSLAALRGWYAGLSSRAAVEHYLAAR
                     KAAGQSARGMLGHIRRQLIVFANSRHRPDLADVFQHAPEERQGRAGAVERAIRLLPTLP
                     APKPQISDSIDQWLSPRTVRALQAYGIHTLSALTVRIPRRRGWWKAIEGLGMTGARQVE
                     AFFAAHPQLTERARALIPIIQPSGIVPWEQLRLPNEVDGSQGNFRAPRNACTLNATNDY
                     EAVQAWLALHETVATQRAYRKEAARLILWAIVERGRALSSLTTDDAIAYRAFVRHPTPR
                     ERWVGPPRPRDSVEWRPFSGGLSARSAAYALTVLSALFRWLIEQRYVLANPFAGVKVRG
                     HALRPALDTARGFTEGEWLLLRAIADGLEWSYGWSEPAAQRLRFMLDFGYATGLRASEL
                     VGAVLGNVRLDEHGDHWLHLVGKGGKPGKVALPPLAYTALDQYLAQRQLPVSRERWNPK
                     TPLVGSLGEDGNAGITGPHLWCVLRRFFTQAAEAIEADHPVAAEKLRRATPHWMRHSHA
                     SHALARGAQLTAVRDNLRHASIATTSMYLHGDELERARQMRQAFGARHVKRGVD"
                     /name="tnpR"
     gene            8553..11582
                     /gene="tnpA"
                     /locus_tag="SLH58_RS00045"
                     /old_locus_tag="CDKHEDKG_00008"
     CDS             8553..11582
                     /gene="tnpA"
                     /locus_tag="SLH58_RS00045"
                     /old_locus_tag="CDKHEDKG_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152392.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family transposase"
                     /protein_id="WP_004152392.1"
                     /translation="MSKKNKLLSVLSDAEQEALYGLPEFDDAQQLEYLAVTETELALAN
                     SRPSLHAKVYCLLQIGYFKAKHAFFSFDWDEVEDDCAFVLSRYFHGEVFEPKAITKHER
                     YTQREQIAKLFGYWPWSAAFLPQLEQQAARTVRRDVTPGFVAAELIVWLNEHKIIRPGY
                     TTLQELVSETLSAERRRLGNLLEQALDESAKAALSQLLVRDDTLSQLAALKQDAKNFGW
                     RQMVREREKRATLEPLHAIAKTLLPKLDVSQQNLLYYASLANFYTVHDLRNLKADQTQL
                     YLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFADEQVRRQQETPRVGRLLS
                     LYVDDSVADPTPFGEVRQRAYKIMPRDTLQTTAQRMSVKPASKLALQWQAVDGLADRMR
                     RHLRPLYVALDLSGSNPDSPWLAALAWAKGVFAKQQRLSQRPLDEWPAATLPKRLRPYL
                     LTFDADGKPTGLHADRYEFWLYRQIRKRLQSGELYLDDSLQHRHFSDELVEMDRKIDVL
                     AKMEIPFLQQPVHAQLDALTAELRTQWLAFNRELKQGKLTHLEYNKDTQKLTWRKPKSE
                     NEKAREKAFYEKLPFCDVADVFRFVNDECKFLSAFTPMQPRYAKKVADADSLMAVIIAQ
                     AMNHGNHVMARTSDIPYHVLDSGYQQYLRQATLHAANDCISNAIATLPIFPYYSFDLET
                     LYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHHV
                     FDIWYRNTSDIMPTAITGDMHSVNKANFAILHWFGRRFEPRFTDLNAQLKELYCADDPA
                     QYQACLIRPVGKIDCDLIHREKPNIDRIVATLGLKEMTQGTLIRKLCTYTTTNPTRRAI
                     FEFDKLIRSIYTLRYLRDPQLERNVHRSQNRLESYHQLRSAIAQVGGKKELTGRTDIEI
                     EISNQCARLIANTIIFYNSAILSRLVTKYEAAGNSKALALITKISPAAWRHILLNGHYT
                     FQSSGKTIDLDAIVAGLELE"
                     /name="tnpA"
     ARG             4101..4741
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1168..2045
                     /name="blaKPC-3"
                     /gene="blaKPC-3"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(5648..5821)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          5800..6291
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 atgaacctcc agcatgagcg gatcgacgcc ctgtgccagc aactcaaact cgaacgggtg
       61 gcaacgcatt accccgtcct ggcgcagcag gcggtgcgcg aggacttcag cttcaccgac
      121 ttcctggaac acctgttgcg gcatgaagcg gggtgtcgcc agcagcgcag ccgcgaactg
      181 ctgacgcgga tggcttgctt cccgggcatc aagaccctgg aggactatga cttcagcttc
      241 aaccccggcg tgcccaaggc actggtgcag gagctgggca gtctggcgtt tgtcgaacgg
      301 gccgagaacg tggtgctgat cggaccctct ggcatcggca agacccacct cgccatcgcc
      361 ttgggttaca aggccgcgca agccggggtc aagacacgct tcatcacggc agccgacctg
      421 atgctgcaac tgagcacggc gcagcgacaa gggcggctga aggaatactt caaccgcgcc
      481 atcctcggtc cacggctgct ggtgatcgat gagatcggct acctgccttt cggacgcgag
      541 gaagcgaacc tgttctttca ggtggtggcg aagcgctatg agaaaggggc gatgatcctg
      601 acctcgaacc tgccgttcag ccagtgggcc gacgcgtttg ccggcgatac caccctgaca
      661 gccgcgatgc tggatcggct gctgcaccat gcccatatcc tgaccctgag cggcgaaagc
      721 taccgcttga aggacaagag gaaggcggga gtggtcagga aaaattccaa acccgaatga
      781 tccaggtggg tcagtattac tttggtgatt caggggtaaa gtgggtcagt tttcagttgg
      841 tgttgacacc ggcgtaccct cggtgctatc ttcgcgcccc aatagtcggg gcttggccag
      901 gacttcctga ggccgtccgt aacgtggatg ccgaggtcag gcgaggtggc cgacccatga
      961 acgccgacct gattcgtttt tcaatagcgc tgggcgttgt ggtgccaggg acttaccaac
     1021 ccgatgtgtg cccatccggg gcagttacag ccgttacagc ctctggagag ggagcggctt
     1081 gccgctcggt gataatccca gctgtagcgg cctgattaca tccggccgct acacctagct
     1141 ccaccttcaa acaaggaata tcgttgatgt cactgtatcg ccgtctagtt ctgctgtctt
     1201 gtctctcatg gccgctggct ggcttttctg ccaccgcgct gaccaacctc gtcgcggaac
     1261 cattcgctaa actcgaacag gactttggcg gctccatcgg tgtgtacgcg atggataccg
     1321 gctcaggcgc aactgtaagt taccgcgctg aggagcgctt cccactgtgc agctcattca
     1381 agggctttct tgctgccgct gtgctggctc gcagccagca gcaggccggc ttgctggaca
     1441 cacccatccg ttacggcaaa aatgcgctgg ttccgtggtc acccatctcg gaaaaatatc
     1501 tgacaacagg catgacggtg gcggagctgt ccgcggccgc cgtgcaatac agtgataacg
     1561 ccgccgccaa tttgttgctg aaggagttgg gcggcccggc cgggctgacg gccttcatgc
     1621 gctctatcgg cgataccacg ttccgtctgg accgctggga gctggagctg aactccgcca
     1681 tcccaggcga tgcgcgcgat acctcatcgc cgcgcgccgt gacggaaagc ttacaaaaac
     1741 tgacactggg ctctgcactg gctgcgccgc agcggcagca gtttgttgat tggctaaagg
     1801 gaaacacgac cggcaaccac cgcatccgcg cggcggtgcc ggcagactgg gcagtcggag
     1861 acaaaaccgg aacctgcgga gtgtatggca cggcaaatga ctatgccgtc gtctggccca
     1921 ctgggcgcgc acctattgtg ttggccgtct acacccgggc gcctaacaag gatgacaagt
     1981 acagcgaggc cgtcatcgcc gctgcggcta gactcgcgct cgagggattg ggcgtcaacg
     2041 ggcagtaagg ctctgaaaat catctattgg cccaccaccg ccgcccttgc gggcggcatg
     2101 gattaccaac cactgtcaca tttaggctag gagtctgcgc ggcagagccg tgtgaccggt
     2161 tttctgtaga gcactgacga tggcggcggc gctctctgca attggcaagg cgtcggcgcc
     2221 aaggatacca atcttgcggc gcgcggcgtg ttatgacgac tggggtgcat ttgagccgcc
     2281 ccatttaacc ttcgccctca cagatacgcc attcgcctca gatttagcgc catgcagacg
     2341 agcttccact cggcttgcac cttgtccagg cccctcatgc tgaactgacg caatcccatc
     2401 accgccttga tccaaccatt cggagcctcc acgatcgact tgcgccggcg gtaagctgca
     2461 tcgccttgct ccgttttcaa tttcgccgca atcgccgccg tatgcggatg ggtcttggca
     2521 ttgaccttgg catcttcacg tccctcgcgg ccgagggcaa cgatgacatc gccgtggtga
     2581 tcggcgacct ttgccagaac agcctcacta cggaatcccg catccgccag cgtctgggcc
     2641 ggcatttctc cggtgttggc ctgaactgct gccagcatgc ccagcagcgc ctgactgtcc
     2701 gcggcgcagt tggtcaactc cgccgccacg atgatctggt gctcggcatc gaccgctgtg
     2761 tacccgttgt agctctgctc ggagccacca ccggcgtgtt tcatgatccg gctgtccgga
     2821 tcggtgaagc tttcctgatc acggtcatcc ggcacaccaa actcgcgttt gtacgagcca
     2881 ccgcccttgt ccgagccatc cggatggcga ggccggcggc catcgtcttc gctgcgcccc
     2941 cgggcctggt ccgcttcacg ctggcgcgct tccaggcgcg cctttgccgc ctggatcgcc
     3001 tccaggcgct tctcgcggcg agaaatctcg gcaggaatgt ccagctccgg ctcgttacgc
     3061 tcctggtcgt cggtagcctt ggcgcgatca agcagcgcct tgatctcgca atgcaattcg
     3121 tcctcggccg gcttcatgcg cttatagctc atcgccttgt ggcggctggc gttggctttt
     3181 accttggtgc cgtcgaccgc gatcgtgcca agcttcacca gcccacattc gcgcgccagt
     3241 tgcaccacct gaacgaacaa attctcaagc tcggtcaggt gtagggcacg gaagtcactc
     3301 agcgtgcggt gggccgggaa gtttccagcg gccagcacac gcaacgcgac atcctcgtac
     3361 agcttcctgg ctagtttgcg cgaagagaag acgccgctcg cgtaaccata gatcagcact
     3421 ttgaccatca tcgccggatg aaacggctga ttgcccggac caccgccggc ataccgggcg
     3481 tggaatgcgc tcaagtccag cgtatcgaca gtctcgctga tgaagtaggc aagatgccct
     3541 tcaggtagcc actctcccag agaagggggc agcagatagg attggtcggg tcggtaagga
     3601 aggtaactgg cagccatccc cgtatcgtcc tcgatcttcc gccgatttgg cttctgccgc
     3661 gcaggctcct aggccaccgt agctaaacat acgagaaaaa ccgcttcttg agcgctctct
     3721 gagaccacct tgccgacaac gcagaaacgg ttgcggaaaa atcggcgttt ccggcgtacg
     3781 ccacccgacc tggacgaact attgcggacc tggaactgga gtagcggaat tccccggggt
     3841 ttcagcgtcg cgccacagcg cctcagatag atgcggtagc ctttggcggg tcgtcatgcc
     3901 gcggaccacc cccccaaaaa attccacaat gcggaatggc ggaattttct ggggttcccg
     3961 cttacccccc cagctacgtg ccttgctgaa gaaacaccag atctaatacg catttgcagg
     4021 tgtcatactg gcgggtttga cgcataacgt cataaggttt accatggcgt cattactgta
     4081 taaaaacaca ggcatagata tgactctggc attagttggc gaaaaaattg acagaaatcg
     4141 cttcaccggt gagaaagttg aaaatagtac attttttaac tgcgattttt caggtgccga
     4201 cctgagcggc actgaattta tcggctgcca gttctatgat cgcgaaagtc agaaagggtg
     4261 caattttagt cgcgcaatgc tgaaagatgc cattttcaaa agctgtgatt tatcaatggc
     4321 agatttccgc aacgtcagtg ccttgggcat tgaaattcgc cactgccgcg cacaaggcgc
     4381 agatttccgc ggtgcaagct ttatgaatat gatcaccacg cgcacctggt tttgcagcgc
     4441 atatatcact aatactaatc taagctacgc caatttttcg aaagtcgtgt tggaaaagtg
     4501 tgagctgtgg gaaaaccgct ggatggggac tcaggtactg ggtgcgacgt tcagtggttc
     4561 agatctctcc ggcggcgagt tttcgacttt cgactggcga gcagcaaact tcacacattg
     4621 cgatctgacc aattcggagt taggtgactt agatattcgg ggtgttgatt tacaaggcgt
     4681 taagttagac agctaccagg catcgttgct catggagcgg cttggcatcg ctgtgattgg
     4741 ttagttcagc gtttacaccg ggagatgcgt ttgacgattt acttttaacc agtacgactc
     4801 ctggcaagca ttgtgaaacg ttttctccag tggacgaata tcctgaccta ccgcatcaac
     4861 gacatgccat ctaatgctgc attctggaca ggaatttgat gaatatctaa ccaattcatt
     4921 ttcgcgagaa caacgaataa ccactcccga atcatgaagc catgtcttaa cgaaaatatg
     4981 gctaactcat agcaaaaaaa aagcccccta agggcttttt tttgtacctg acggtcagac
     5041 cgattaaaca taacatccct tgcccgcacc cccgcaggcg ccctcagcgc aatcctgctg
     5101 catcagggcc gtttccagca cctttcctca ccagtatccc ggaaaaacgc ccctgatgat
     5161 ttctgcgcga ctgcatggct atgcacgcag gtgaatggca ggtgatttcg cagcgtcagc
     5221 accactaaac cgccaaaagc caaaatcagc ggcgcggcgc gcgcggacgt gaccaggcaa
     5281 aatcggactg ctgccgctca tcctcacgta atgcacgctg gcggtcggcg taacgctgca
     5341 actcaatttg cgcacgcgct ctgttcagga cgatgtgccg gttaggctga tttagctttt
     5401 tacctgacgc tctttcagct gcggcaacag catcatcaag aaattttttc tctgcaggcg
     5461 tcaagtttgt cagtctggcc ataacgttct ccagtcagaa taaatcagca cagtctgcca
     5521 gacacagtag aaacgatgaa gcgccagcga cggtgaaagg cagtcagaaa cggtgaacgg
     5581 gagcggattt taatccggtg ccgtgaggcg ttggacaagc cgcaggcgcg tcattagctt
     5641 ttagccggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg
     5701 gctgactatg ttggcacgga tgtggaaatc agtgaagtgc ttcatgtagc aggagaaaaa
     5761 aggcggcacc agcgcgtcag caggatatgt agtacaggat atattccgct tcctcgctca
     5821 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt gccggcttac aggcggggcg
     5881 gaaatttcct gggagatacc aggaaggagc ttaacaggga agagataagg ccgcggcgaa
     5941 gccgtttttc cataggctcc gcccccctga caaacatcat gaaatctgac gctcaaatca
     6001 gtggtggcga aacccgatgc ggcttaaaga taccaggcgt ttccccctgg tggctccctc
     6061 gtgctccccc gcattcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
     6121 ttatctcatt ccacgcctga cactcagttc cgcgaaggca gttcgctcct ggatggactg
     6181 tatgcacgaa ccccccgttc agtccgactg ctgcgcctta tccggtaact atcatcttga
     6241 gtccaacccg gaaagacacg acttatcgcc actggcagca gccactggta actgatttag
     6301 aggagttagt cttgaagtta tgcgcctgtt aaggctaaac tgaaagaaca gatttggtta
     6361 ctgcgctcct cgtaagccag ttaccacggt tcaaagagtt gctgctctga gaaccttcga
     6421 aaaaccgcct tgcagggcgg ttttttcgtt tacagagcaa gagattacga cgccacagaa
     6481 aggatctcaa gaagatcctt caccgtaatg gaaatctatc caatccgagc acataggata
     6541 cacatcgtta taacgctgga tctgcgtgaa ttccagcagc agcaagagaa agactttcta
     6601 caaaccagct tacagcaggc taaatttaac cagaaaaaag ccgctgaatt actgggtctg
     6661 acttatcatc agctaggggt tctaagcggg aatcccagaa aattccgtca ttccgtatga
     6721 tgaaaattca gtcgactcct cgcttgacgt gacgagcgcc aaaagcctgc cgcatctgcc
     6781 tggcgcgctc gagctcatcg ccatgcaaat acatcgacgt cgtggcgatc gacgcatggc
     6841 gtaggttgtc gcgcaccgcg gtgagctgag ctccccgggc caacgcatgg ctggcatgac
     6901 tgtgacgcat ccaatgaggc gtggcacggc gcagcttttc ggcggcgacc ggatgatcgg
     6961 cctcaatggc ctcagcggct tgcgtaaaga atcttcgcaa cacgcaccaa aggtgagggc
     7021 cggtgatgcc ggcattaccg tcttccccaa gactgcccac caggggcgtt ttcggattcc
     7081 accgttcccg gctgactggc agttgacgct gtgccaagta ttggtccagc gccgtatacg
     7141 cgagcggcgg caaggccacc ttacctggtt tgccaccttt gccaaccaaa tgcagccagt
     7201 ggtcaccgtg ctcgtcgagg cggacattac ccaggacggc gccgaccagt tcgctagcgc
     7261 gcaggcccgt ggcatagccg aagtcgagca tgaagcgcag gcgttgtgct gccggctcgg
     7321 accagccgta ggaccattcc aggccgtctg caatggcgcg cagcagtagc cattctccct
     7381 cggtaaagcc tcgcgccgta tccagtgcgg gccgcaaggc gtgaccgcgt accttgacgc
     7441 cggcgaaagg gttggccagc acatagcgct gctcgatgag ccaccggaac agcgccgaga
     7501 gcaccgtcag cgcatacgct gccgagcgtg cggacaagcc gcccgagaag gggcgccatt
     7561 ccaccgaatc gcgtggtcga ggtggcccca cccaacgctc gcgcggggtc gggtgccgga
     7621 caaaggcccg gtacgcgatt gcatcgtccg tggtcaggga tgatagcgcc cgtccgcgtt
     7681 cgacgatggc ccacaggatt agccgcgcag cctccttgcg ataggcgcgc tgtgtggcga
     7741 cagtctcgtg cagcgccagc caggcctgca cggcctcgta gtcgttggtc gcattcagcg
     7801 tgcaagcgtt gcgcggggcc cggaaattgc cctgcgagcc gtcgacttcg ttcggcagac
     7861 gcaactgctc ccacggcacg atgccgctgg gctgaataat cgggatcagg gcccgggcgc
     7921 gctcggtgag ctgcggatgt gcggcaaaga aagcctcgac ctgccgggcg cctgtcatgc
     7981 cgagtccctc gatggctttc caccagccac gccggcgggg aatgcgcacg gtcagcgccg
     8041 agagggtgtg gataccgtac gcctgcagtg ccctgacggt cctcggagac agccactggt
     8101 cgatgctgtc gctgatctgg ggcttcggcg ccggcagcgt gggcaagagc cggatcgcgc
     8161 gttcgacggc acccgcgcgc ccctgtcgct cttcgggggc gtgctggaac acgtcggcca
     8221 gatccggccg gtggcggctg ttggcgaaaa cgatgagctg gcggcggata tgccccagca
     8281 tccctcgcgc tgattgaccg gcggccttgc gtgccgccag gtagtgttca acggccgcgc
     8341 gagagctgag tcccgcgtac cagccgcgca aggcagccag cgaatccgca tcgggaaagc
     8401 cgactggggt agaactcgaa gccgtagtct ggcccgtttt catatggaca ggttgcgatt
     8461 cgaaaagcga gtaacgcgat aataacgatt atttcagtag aatcgaaaat gccccatgtt
     8521 tctacgaaac ctcaagatag caagaacggg ccatgagcaa gaagaacaag ctactcagcg
     8581 tcctatccga cgccgaacag gaggccttgt atggcctgcc ggagttcgat gatgcgcagc
     8641 agctggaata tctggctgtg acagaaaccg agttggcgct tgccaacagc cggcccagtc
     8701 ttcatgccaa ggtctattgc ctcttgcaga tcggctactt caaggccaag catgccttct
     8761 tcagtttcga ctgggacgaa gtcgaggacg attgcgcttt cgtgctgagc cgctatttcc
     8821 acggcgaggt gttcgaaccc aaggcgatca ccaagcatga acgctacacc cagcgcgagc
     8881 agatcgccaa actattcggc tactggccgt ggtcggccgc cttcctgccg cagctcgagc
     8941 agcaggccgc acggaccgtg cggcgtgacg taacgccggg gttcgtcgcc gccgagctga
     9001 tcgtctggct caatgagcac aagattatcc ggcccggcta tacgaccttg caagagttgg
     9061 tcagcgaaac gctatccgcc gagcgccggc gcctgggcaa tctgctggag caagcgttgg
     9121 acgaatctgc caaggccgcg ctgagccagc ttctggtccg tgatgacacc ctgtcgcaac
     9181 tggcggcgct caagcaggac gccaagaatt tcggctggcg ccagatggtc cgcgagcgcg
     9241 aaaagcgcgc cacgctggag ccgctgcacg caatcgccaa gacactgctg cctaaactcg
     9301 acgtctcgca gcagaacctg ctgtactacg cgagcctggc aaacttctac accgtccacg
     9361 acctgcgcaa tctgaaggcc gatcagaccc aactctacct gctgtgctat gcctgggtac
     9421 gctaccggca gctctccgac aacctggtcg atgcgatggc gtaccacatg aagcagttgg
     9481 aggacgaaag cagcgctggt gccaaacaat cctttgccga cgagcaggtg cgccgtcagc
     9541 aggaaacgcc gcgggtaggc cgcctgctgt cactctacgt tgatgatagc gtggccgatc
     9601 ccacgccctt cggcgaggtg cggcagcgcg cctacaagat catgcctcgc gatacgctgc
     9661 aaaccacggc gcagcgtatg agcgtgaagc cggcgagcaa gctggccttg cagtggcagg
     9721 cggtggacgg gctggccgac cgcatgcgcc gccatcttcg tccactgtac gttgcgctcg
     9781 acctctccgg ctccaatcca gacagtccat ggcttgccgc gctggcctgg gccaaaggtg
     9841 tttttgccaa gcagcagcgg ctgtcgcaac ggccactcga cgagtggccc gcggcgacac
     9901 ttccgaaacg cttgcggccg tacctgctga ccttcgacgc cgacggtaaa ccaaccggcc
     9961 tgcacgctga ccgctacgag ttctggctgt accgccagat caggaagcgt ctccaatcgg
    10021 gtgaactcta cctcgacgac agcctgcaac accggcactt ctcagatgaa ctggttgaga
    10081 tggacaggaa gatcgatgtg ctcgcaaaaa tggagatccc cttcctgcag caacccgtcc
    10141 atgcccaact cgatgcgctg actgccgaac tgcgcacgca gtggctggcg ttcaaccgcg
    10201 agctgaaaca gggcaagctg acgcacctcg aatacaacaa ggacacgcag aaactgacct
    10261 ggcgcaagcc caagagcgag aatgagaaag cgcgcgaaaa ggcattctac gagaagcttc
    10321 cgttctgcga cgtggccgac gtgtttcgct tcgtcaacga cgagtgcaag tttctgtcgg
    10381 cgttcacgcc catgcagccg cgctacgcga agaaggtcgc cgacgctgac agcctgatgg
    10441 cggtcatcat cgcgcaagcg atgaaccacg gcaaccacgt catggcgcgc accagcgaca
    10501 tcccgtacca cgtgctggat agcggctacc agcagtacct gcgccaggca acgctgcacg
    10561 cggccaacga ctgcatcagc aacgccatcg ccacgctacc gatcttccca tactactcgt
    10621 tcgacctcga aacgctgtac ggtgccgtcg acgggcagaa attcagcgtc gaacggccga
    10681 ccgtgaaggc gcgctattcg cgcaagtact tcgggcgcgg caagggcgtg gtcgcctaca
    10741 cgctgctgtg caaccacatc ccgctcaacg gctacctgat cggcgcgcac gactacgagg
    10801 cccaccacgt gttcgacatc tggtatcgca acacgtcgga catcatgccg accgcgatca
    10861 ccggcgacat gcacagcgtc aacaaggcca acttcgccat cctgcattgg tttggccggc
    10921 ggttcgagcc gcgcttcacc gacctcaacg cgcaattgaa agaactctac tgcgctgacg
    10981 atccagcaca gtatcaggcg tgcctgattc ggccagtcgg gaaaatcgac tgcgatctca
    11041 tccatcgcga gaagccgaac atcgaccgga tcgtcgccac actcgggctg aaggaaatga
    11101 cgcagggcac gctgatccgc aagctgtgca cctacaccac gacgaacccg acgcggcgcg
    11161 cgatcttcga gttcgacaag ctcatccgca gcatctacac gcttcgctac ctgcgcgatc
    11221 cgcaactgga acgcaacgtg caccgctcgc aaaatcgcct cgagtcctac catcagctac
    11281 gctcggccat tgcccaggtc ggcggtaaga aggaactgac cggtcgcacc gacattgaaa
    11341 tcgagatcag caaccagtgc gcacggctga tcgccaacac gatcattttc tacaactcgg
    11401 caatcttgtc gcggctggtg accaagtacg aggcggccgg caacagcaag gcgctggcac
    11461 tcatcacaaa aatttcgcct gcggcatggc ggcacatcct gctcaacggg cactacacct
    11521 ttcaaagcag cggcaagacg atcgatctcg atgcgatcgt cgcaggcctg gaactggagt
    11581 gacggaattt tcagcggttc cgtgttagca gcagtgtaaa actgacccac cccagcgaag
    11641 taaaactgat ccacctgggc tagtttggcg gccttttcga ggccgtcgat gttgacccag
    11701 gagcaaaccg tggaaatcag agttcttgcc cgacagggcc actccatccg gcacatcgcc
    11761 cgcacgttgg gggtctcgcg caataccgtg cgccgctacc tccgcgatcc gtcggtagcg
    11821 cgataccagc cccgtgaacc acgccccacc aaactggggc catttgaatc ttatctgcgg
    11881 caacgggtcg agcaggccca ccccatctgg ctgcctgcca ccgtcctgaa tcgggaaatc
    11941 cgcgctcaag ggtatggcgg cggtctctcg ttattgcgcg ccttcctcgc cacgctcaag
    12001 cccgcccgcc gcgaagcagg tccggccgtc cgtttcgaga ccgagcccgg acggcagttg
    12061 caggctgatt tcgtggtctt gcgccgggcc agatccccca tgtcggcctt cgtcgccacg
    12121 ctgggataca gccgcatgac gtttgtcacc ttcgtaccgg acgaatcctt cgagtccgtg
    12181 cgggactccc tgttgctggc ttttgactat ctgggcggtg tgccccgcga ggtgttgttc
    12241 gacaacatga agaccgtggt gctggaacgc gatgcctacg gtgacggcaa gcaccgtttt
    12301 caccccggct tgcttcaact ggccgatgac ctggggttcc ggatccggct gtgtcgcccg
    12361 tatcgggccc agaccaaagg caaggtcgag cgcttcaatc gctacttccg cgagagcttc
    12421 tacaacccgt tgctgacccg gatgaagggc acggggctgc tgctcgattg tgctgctgcc
    12481 aaccgacgcg tccgtgactg gttggcagac gaggcgaatg tgcgggtcca cgccaccctg
    12541 aacgagcgcc ccatcgaccg ttggcggcag gagcgggaac acctgcaacc cttgccatcc
    12601 cgtgtgcggc gcgacgaggc ccccctgctg gataacagtc tccgcccggt accactggaa
    12661 tctctccagc acccactgtc tgtctatgac gcaattgggg aggcctgccg
//
