LOCUS       NZ_OQ787038             3191 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Escherichia coli strain SN25556 plasmid pAM1, complete sequence.
ACCESSION   NZ_OQ787038
VERSION     NZ_OQ787038.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Furmanek-Blaszk,B.
  TITLE     Escherichia coli strain SN25556 plasmid pAM1 complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Furmanek-Blaszk,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2023) Microbiology, University of Gdansk, Wita
            Stwosza 59, Gdansk, Pomerania 80-309, Poland
COMMENT     ##Assembly-Data-START##
            Sequencing Technology             :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/14/2023 06:31:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OQ787038.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SN25556"
                     /isolation_source="cloacal swab"
                     /host="Eurasian coot Fulica atra"
                     /db_xref="taxon:562"
                     /plasmid="pAM1"
                     /geo_loc_name="Poland"
     gene            complement(52..201)
                     /locus_tag="RA110_RS00005"
     CDS             complement(52..201)
                     /locus_tag="RA110_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846302.1"
                     /translation="MPSEYDEQRGSTDTQLPGEYDLFDLMAKVTEEILHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            <1139..1294
                     /locus_tag="RA110_RS00010"
                     /pseudo=""
     CDS             <1139..1294
                     /locus_tag="RA110_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            1388..2032
                     /locus_tag="RA110_RS00015"
     CDS             1388..2032
                     /locus_tag="RA110_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     ARG             1389..2029
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(237..410)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          389..880
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 aaataaaagg taataccggc gtattaccac gtacgcgggc aagcgaaaaa gctataacgc
       61 ttcccggccg acaggcggcc cagaatccac aggggcatgc aggatctctt cagtaacctt
      121 cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg aaccccgttg
      181 ttcatcatat tcagatggca tggggtgata tccagtagca gagaggttgt tagcgggttt
      241 cggggcgcag ccctgaacca gtcacgtagc gctagcggag tgtatactgg ctgactatgt
      301 tggcacggat gtggaaatca gtgaagtgct tcatgtagca ggagaaaaaa ggcggcacca
      361 gcgcgtcagc aggatatgta gtacaggata tattccgctt cctcgctcac tgactcgctg
      421 cgctcggtcg ttcggctgcg gcgagcggtg ccggcttaca ggcggggcgg aaatttcctg
      481 ggagatacca ggaaggagct taacagggaa gagataaggc cgcggcgaag ccgtttttcc
      541 ataggctccg cccccctgac aaacatcatg aaatctgacg ctcaaatcag tggtggcgaa
      601 acccgatgcg gcttaaagat accaggcgtt tccccctggt ggctccctcg tgctcccccg
      661 cattcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tatctcattc
      721 cacgcctgac actcagttcc gcgaaggcag ttcgctcctg gatggactgt atgcacgaac
      781 cccccgttca gtccgactgc tgcgccttat ccggtaacta tcatcttgag tccaacccgg
      841 aaagacacga cttatcgcca ctggcagcag ccactggtaa ctgatttaga ggagttagtc
      901 ttgaagttat gcgcctgtta aggctaaact gaaagaacag atttggttac tgcgctcctc
      961 gtaagccagt taccacggtt caaagagttg ctgctctgag aaccttcgaa aaaccgcctt
     1021 gcagggcggt tttttcgttt acagagcaag agattacgac gccacagaaa ggatctcaag
     1081 aagatccttc accgtaatgg aaatctatcc aatccgagca cataggatac acatcgttat
     1141 aacgctggat ctgcgtgaat tccagcagca gcaagagaaa gactttctac aaaccagctt
     1201 acagcaggct aaatttaacc agaaaaaagc cgctgaatta ctgggtctga cttatcatca
     1261 gctacgtgcc ttgctgaaga aacaccagat ctaatacgca tttgcaggtg tcatactggc
     1321 gggtttgacg cataacgtca taaggtttac catggcgtca ttactgtata aaaacacagg
     1381 catagatatg actctggcat tagttggcga aaaaattgac agaaatcgct tcaccggtga
     1441 gaaagttgaa aatagtacat tttttaactg cgatttttca ggtgccgacc tgagcggcac
     1501 tgaatttatc ggctgccagt tctatgatcg cgaaagtcag aaagggtgca attttagtcg
     1561 cgcaatgctg aaagatgcca ttttcaaaag ctgtgattta tcaatggcag atttccgcaa
     1621 cgtcagtgcc ttgggcattg aaattcgcca ctgccgcgca caaggcgcag atttccgcgg
     1681 tgcaagcttt atgaatatga tcaccacgcg cacctggttt tgcagcgcat atatcactaa
     1741 tactaatcta agctacgcca atttttcgaa agtcgtgttg gaaaagtgtg agctgtggga
     1801 aaaccgctgg atggggactc aggtactggg tgcgacgttc agtggttcag atctctccgg
     1861 cggcgagttt tcgactttcg actggcgagc agcaaacttc acacattgcg atctgaccaa
     1921 ttcggagtta ggtgacttag atattcgggg tgttgattta caaggcgtta agttagacag
     1981 ctaccaggca tcgttgctca tggagcggct tggcatcgct gtgattggtt agttcagcgt
     2041 ttacaccggg agatgcgttt gacgatttac ttttaaccag tacgactcct ggcaagcatt
     2101 gtgaaacgtt ttctccagtg gacgaatatc ctgacctacc gcatcaacga catgccatct
     2161 aatgctgcat tctggacagg aatttgatga atatctaacc aattcatttt cgcgagaaca
     2221 acgaataacc actcccgaat catgaagcca tgtcttattt ataatctcaa cttcagccat
     2281 gtcatcccgt ttactgacat cctcaataca aataccccgc gatcatcgcg tttattgagt
     2341 ttattattta tctatgcgat tccgatgaaa aattcgcacg aaaatatggc taactcatag
     2401 caaaaaaaaa gcccttaggg ggcttttttt tgtacctgac ggtcagaccg attaaacata
     2461 acatcccttg cgtgcaccgc ctgagaggct ctgagggcgt ttctgacggg aaaccggtaa
     2521 aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat
     2581 gcatgagtat gcgcgcaagt gcatagcgcg gaaattcaca ccattcagcc aggggaagtc
     2641 ctgtttccgg cgttcttccc atccgaaaac ggtgaacggg gacgcaattt attgcgtggc
     2701 cgtgaagcgt tgccggcgca gccggaaaac ccgctgtcag cacgttcagg aagggggtga
     2761 tgtacaaatg gcacagagga tgaaagtgac gtatgagctg aaggaatgat gtaaaagtga
     2821 tgtataaatg atgtaaaagt gatgcaaaag tgttgtaaaa gtgatgtaca ggccgaagga
     2881 gtgttgtaaa agtgttgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     2941 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc ttttttttgt
     3001 cacgatctcc ccttaacctg cgtaaatcac ccgccatggt tgattatttt tcaacctcaa
     3061 aatggtcata cacctaacgc agccgcgatt ttcatcgcaa ctgcatgaat taaaagaaaa
     3121 aaaagtaatg tttcgttttc acgagtaata cccaggtatt aaaaacccac tgtaattaca
     3181 tgaaaataaa g
//
