LOCUS       NZ_OP435782             6156 bp    DNA     circular CON 29-APR-2024
DEFINITION  Klebsiella michiganensis strain K92 plasmid pK92-NR, complete
            sequence.
ACCESSION   NZ_OP435782
VERSION     NZ_OP435782.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Luo,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2022) Department of Clinical Laboratory, Taizhou
            Municipal Hospital, No. 381-1, Zhongshan East Road, Jiaojiang,
            Taizhou, Zhejiang 318000, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.5
            Sequencing Technology             :: nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028864595.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:02:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 5
            CDSs (with protein)               :: 5
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP435782.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="K92"
                     /db_xref="taxon:1134687"
                     /plasmid="pK92-NR"
                     /geo_loc_name="China: Taizhou"
                     /collection_date="2017"
     gene            join(6090..6156,1..914)
                     /locus_tag="PYH76_RS00005"
     CDS             join(6090..6156,1..914)
                     /locus_tag="PYH76_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.2"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="WP_064349473.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRRFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
                     /name="IS5-like element IS5 family transposase"
     gene            <1061..1684
                     /gene="aqpZ"
                     /locus_tag="PYH76_RS00010"
                     /pseudo=""
     CDS             <1061..1684
                     /gene="aqpZ"
                     /locus_tag="PYH76_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226086.1"
                     /GO_function="GO:0015250 - water channel activity [Evidence
                     IEA]"
                     /GO_process="GO:0006833 - water transport [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /name="aqpZ"
     gene            complement(1894..2607)
                     /locus_tag="PYH76_RS00015"
     CDS             complement(1894..2607)
                     /locus_tag="PYH76_RS00015"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_071600436.1"
                     /translation="MMDNKPLNKKELESIFLTSGAFNPKGLYLGEGFRKQALELGFPPY
                     ILPTEINIVLHYSPELSVRTFVSTLWNTGASLTEVIHLERKSFVFKSPRPYVKVLSSET
                     ESLSLDNDNRLSPRRKIPLLSPYYIAQVKLLIATLKIAGNTCGDSTKPRRIWDVDNFIA
                     TSWIDNAIKHARLDGVIFPVEVTPDAIKHAFAMNMLILGVEPSIVMKLMGFKRNKWLEI
                     YLKVKCLIMSLERDM"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(2666..3769)
                     /locus_tag="PYH76_RS00020"
     CDS             complement(2666..3769)
                     /locus_tag="PYH76_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="WP_017901372.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEIYNKDGNKLDLYGKIDGLHYFS
                     DDKSVDGDQTYMRIGVKGETQINDQLTGYGQWEYNVQANNVEGSDKQSWTRLAFAGLKF
                     GDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRANGVATYRNSDFFGLVD
                     GLNFALQYQGKNGSVSGEGQTNNGRGWNKQNGDGFGTAITYDIWEGISAGFAYSHSKRT
                     DDQNAAGLVGRGKYAETYTGGLKYDANNIYLAAEYTQTYNATRFSGDSVSGYANKAQNF
                     EVVAQYQFDFGLRPSVAYLQSKGKDIEGFGDQDLLKYVDVAATYYFNKNMSAYVDYKIN
                     LVDDNRFTSAAGISTDDIVALGLVYQF"
                     /name="porin OmpC"
     gene            <3993..4082
                     /locus_tag="PYH76_RS00025"
                     /pseudo=""
     CDS             <3993..4082
                     /locus_tag="PYH76_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901096.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /name="DinI-like family protein"
     gene            complement(4098..4283)
                     /locus_tag="PYH76_RS00030"
     CDS             complement(4098..4283)
                     /locus_tag="PYH76_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017901373.1"
                     /translation="MSRVTAIISAVVMLNGERVDDDRLQLDTNPLLMDAISAVKYLAGK
                     MEQLQNIIAELKASNA"
                     /name="hypothetical protein"
     gene            complement(4372..>4732)
                     /locus_tag="PYH76_RS00035"
                     /pseudo=""
     CDS             complement(4372..>4732)
                     /locus_tag="PYH76_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383026.1"
                     /GO_function="GO:0003796 - lysozyme activity [Evidence
                     IEA]"
                     /GO_process="GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]; GO:0009253 - peptidoglycan
                     catabolic process [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /name="glycoside hydrolase family protein"
     gene            complement(4808..5839)
                     /locus_tag="PYH76_RS00040"
     CDS             complement(4808..5839)
                     /locus_tag="PYH76_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element ISEc33 family transposase"
                     /protein_id="WP_001752311.1"
                     /translation="MPIIVPIPRGERRLMQKAIHKTRDKNHARRLTAMLMLHRGERVSD
                     VARTLCCARSSVGRWINWFTHSGIEGLKSLPAGRSRRWPFEHICTLLRELIKHSPGDFG
                     YQRSRWSTELLAIKINEITGCQLHAGTVRRWLPSAGLVWRRAAPTLRIRDPHKDEKMAV
                     IHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSG
                     TGKVSYVGGNSKSSALFIALLKHLKATYRRAKTITLIVDNYIIHKSRETQRWLKANPKF
                     RVIYQPVYSPWVNHVEQLWQALHDTITRNHQCRSMWQLLKKVRHFMETASPFPGGKHGQ
                     AKV"
                     /name="IS630-like element ISEc33 family transposase"
ORIGIN
        1 ttttcttgtc ccgtatggag cagattctgc catggcaaaa catggtggaa gtcatcgagc
       61 cgttttaccc caaggctggt aatggccggc gaccttatcc gctggaaacc atgctacgca
      121 ttcactgcat gcagcattgg tacaacctga gcgatggcgc gatggaagat gctctgtacg
      181 aaatcgcctc catgcgtcgg tttgcccggt tatccctgga tagcgccttg cctgaccgca
      241 ccaccatcat gaatttccgc cacctgctgg agcagcatca actggcccgc caattgttca
      301 agaccatcaa tcgctggctg gccgaagcag gcgtcatgat gactcaaggc accttggtcg
      361 atgccaccat cattgaggca cccagctcga ccaagaacaa agagcagcaa cgcgatccgg
      421 agatgcatca gaccaagaaa ggcaatcagt ggcactttgg catgaaggcc cacattggtg
      481 tcgatgccaa gagtggcctg acccacagcc tggtcaccac cgcggccaac gagcatgacc
      541 tcaatcagct gggtaatctg ctgcatggag aggagcaatt tgtctcagcc gatgccggct
      601 accaaggggc gccacagcgc gaggagctgg ccgaggtgga tgtggactgg ctgatcgccg
      661 agcgccccgg caaggtaaga accttgaaac agcatccacg caagaacaaa acggccatca
      721 acatcgaata catgaaagcc agcatccggg ccagggtgga gcacccattt cgcatcatca
      781 agcgacagtt cggcttcgtg aaagccagat acaaggggtt gctgaaaaac gataaccaac
      841 tggcgatgtt attcacgctg gccaacctgt ttcgggcgga ccaaatgata cgtcagtggg
      901 agagatctca ctaaaaactg gggataacgc cttaaatggc gaagaaacgg tctaaatagg
      961 ctgattcaag gcatttacgg gagaaaaaat cggctcaaac atgaagaaat gaaatgactg
     1021 agtcagccga gaagaatttc cccgcttatt cgcaccttcc cttgcggcag cattcccggg
     1081 attaggaatt ggttttgctg gggtggcgct ggccttcggt ttaacggtat taaccatggc
     1141 atatgccgtc ggtcatattt ccggcggaca ttttaacccg gcagtgactt taggtttatg
     1201 ggctggaggt cgtttcccgg ccaaagacgt aattggttat attatcgccc aagtcgtcgg
     1261 cggtattatc gccgccgcta ttctgtatgt tgtcgccagc ggtaaagcgg gcttcgacgc
     1321 caccgctagc gggttcgctt cgaacggtta tggcgaacac tctccgggcg gcttctcaat
     1381 gctttccgcc attgtgatag agattgtcct gacatgtggc ttcctgctgg ttattcacgg
     1441 ttccacggat aaaagcgctc ctgccggctt cgcccctatc gccattggac tggcactgac
     1501 gcttattcac ctgatcagca ttccggtcac caacacgtcg gtaaacccgg cgcgtagcac
     1561 tgcggtagcg atttttcagg gcggttgggc attgcaacaa ttatggttat tctgggtcat
     1621 gccaatcatt ggtgggatac tgggcggttt aatttatcgc actttgctca gcagtgataa
     1681 atgaataatt aaaaactcta tacgactgca gatcattcca tattcacgcc agccatcgaa
     1741 aaaatcttca gtcctcccca ctctgcaggc cttgcataca actgtatggg tggggagtgg
     1801 ggggttaaac cgtacaatta tccagcctcg tatagttgat cgagtgtgtc gcaatttcga
     1861 gcacctcaac atgatccaaa gcatcgcttt ttatcacata tccctctcca aggacataat
     1921 taagcattta actttcaaat atatttcaag ccacttattc cttttaaacc ccattaactt
     1981 cattactatt gatggctcaa ctcccaagat cagcatattc attgcaaatg cgtgtttaat
     2041 tgcatccggc gtgacttcta ctggaaaaat cacgccatcc aatctggcat gtttaatagc
     2101 attatctatc catgatgtcg caatgaaatt gtccacatcc catatcctcc taggtttggt
     2161 ggaatcgccg caagtattac ccgctatttt caaagtagca atcaataact taacttgagc
     2221 tatataatat ggggaaagta aaggaatttt ccggcgggga gacaatctat tatcgttatc
     2281 taacgacaat gattcagttt cagaacttaa tactttaaca tatgggcgag gagatttaaa
     2341 aacaaaagac ttccgttcta aatgaatcac ttcagttaat gaagcaccag tattccataa
     2401 agtagaaaca aaagttcgta cactcaactc aggcgaataa tgcaaaacaa tgttaatctc
     2461 tgtgggtaaa atatatggag gaaaacccaa ttcaagggcc tgtttacgaa acccctcccc
     2521 cagatacaac cccttcggat taaaagcccc tgaagtaagg aaaatacttt caagttcttt
     2581 tttatttaat ggcttattat ccatcatatc ttatccatca tatcgacaca aacaccggcc
     2641 accaaggccg gtgtttggta aataattaaa actgatatac taaaccaagt gcgacaatgt
     2701 catcagtaga gatgccagca gcactggtga aacgattgtc atccaccaaa ttgattttat
     2761 aatcaacata agcagacatg tttttgttga agtagtaggt cgcggctaca tcaacatatt
     2821 tcagcagatc ctgatcgccg aaaccttcga tgtctttacc tttggactgc aggtatgcca
     2881 cggacggacg cagaccgaag tcgaactggt actgtgcaac cacttcaaag ttttgtgctt
     2941 tattagcata accactgacg gaatcaccac tgaaacgagt cgcattataa gtttgggtat
     3001 actccgcagc caagtaaata ttgttcgcgt catatttcaa gccaccagtg taagtttctg
     3061 cgtatttacc tcgaccaacc agacctgcag cattctggtc atcagttcgt ttagaatgag
     3121 agtatgcaaa acctgcgctg atgccttccc aaatatcata agtgatcgct gttccgaagc
     3181 catcaccgtt ctgtttgttc cagccgcgac cattgttagt ctggccttca ccacttacac
     3241 taccgttttt accctggtac tgcagcgcaa agttcaggcc gtcaaccaga ccgaagaagt
     3301 cagagttacg ataggttgca acaccattag cacgctgctg catgaagttg tcagagccat
     3361 aagtgtcacc gccaaactct ggcagaacgt cagtccagga agttacgtcg tagacaacgc
     3421 cgtagttacg accgtagtcg aaagagccca cgtcgccgaa tttcagacca gcaaatgcca
     3481 gacgagtcca ggactgcttg tcagaacctt caacgttgtt cgcctgaacg ttgtattccc
     3541 actgaccgta gccagtcagt tggtcattaa tctgggtttc gcccttcacg ccgatacgca
     3601 tgtaggtctg atcgccatcg acgctcttgt catcagaaaa gtaatgcaag ccatcaattt
     3661 taccgtacag gtctaatttg ttgccatctt tgttataaat ttcagccgca tttgctgcac
     3721 ctgctaccag cagagccggt accaagaggg atagtacttt aactttcatg gttgaaccct
     3781 cgttttatat ttgccactgc gtatcgggct gtcattgtat aattatctat acacaaaaaa
     3841 atccagcctt ctcatgctgt aagctagtta aaagttccct gtgaatacac ctacattttg
     3901 taacatattg taaaaggaat gcataacacg tcatctcgtg tctcataacc aaaacacata
     3961 gcaataggaa tggacatgcc ctgaggggct atacagatgc cagtaagcat gaaaaagaac
     4021 aaattggcag agttatcctg gagatgttcg aagaggctga tatgtggatg tctggagaat
     4081 aaaattagcg ataccaatca tgcatttgat gccttcagct ctgcaatgat gttttgcaat
     4141 tgctccattt tgccagctaa atatttcacc gcgctgatcg catccatcag cagcggattg
     4201 gtatcgagtt gcaagcgatc atcgtccact ctctcgccgt tgagcataac cacagcgctg
     4261 ataatggcgg tcacgcggct catgacatga ttattccaac agccagaaac cacggccacg
     4321 catcattacc atctaaggca agaaacgcag caatgacgaa gcaaatcaga ttcatttcac
     4381 accatccaga cagagctgct tttccgctgc gcgacgagtc accaggccgg gaaggatttt
     4441 tcctccgtcg tataccccag cgtgggaact ggttgcaggc actagttatc tttccctgtc
     4501 gcaggtaatt aaacagagtt gatttctgca tactggcgca accagcgtta aacgtgatcg
     4561 acgttacagc agaaaatgta ttgtcgctta gttgtcgtcc attgccgtag gtattgacgc
     4621 agcgctcagc cgcaaggatg tttcgctccc ggtctgcggc tatctgctaa tcagttttgc
     4681 acacaccgcc tttaacgtca tgcgtgttaa ggcacagccc tgatgtaggt agtgattatg
     4741 ctaatagtaa cggacataaa aaagcctgaa tcgccacgga taatctaaat agctgcgcct
     4801 aataccgcta cacttttgcc tgaccatgtt ttcctccggg gaatgggctg gcggtttcca
     4861 taaaatggcg gacctttttc agtaactgcc acattgagcg gcactgatga ttacgggtta
     4921 tcgtgtcatg aagtgcctgc catagctgtt ccacatgatt cacccacggc gagtaaaccg
     4981 gctggtaaat taccctgaac ttgggatttg ctttcaacca gcgctgtgtt tcgcggcttt
     5041 tatggataat gtagttatca acgatcagcg tgattgtttt cgcccgccga taagtggctt
     5101 tcaggtgctt cagcagagcg ataaacagcg ctgaactttt gctgttgccg cccacgtagc
     5161 tgactttacc cgtgccactg tgcagtgcgc cggccagata gtatttttcg ttctgccccg
     5221 gcgtcactac ccgtttctgc tgtccgcgca actgccagtc cgcaccgatt ttaggattaa
     5281 ggtggatatc cacttcatct tcataaaata ccggatgctc tgcgctgcat tcatccagcg
     5341 ctttgtggat taccgccatc ttttcatctt tatgtgggtc acggatacgc agagttggcg
     5401 cggccctgcg ccatacaagc cccgcagatg gcaaccagcg gcgaacggtt cctgcatgta
     5461 actggcaacc ggttatctca ttgattttta ttgccagtaa ttcggtgctc cagcgtgaac
     5521 gttgataacc aaaatcgccg ggagaatgct ttatcagctc acgtaacagg gtgcagatat
     5581 gttcaaaagg ccagcgtcgg gagcgccctg cgggtaagga tttcaggcct tcaatacctg
     5641 agtgcgtaaa ccagttaatc cagcgaccaa cggatgaacg ggcacaacag agagttctgg
     5701 caacatcgct gacccgttca ccccgatgaa gcatcagcat ggccgtgagt ctgcgggcat
     5761 gatttttatc acgcgtttta tgaatagctt tctgcatcag gcgtcgttcg ccacggggta
     5821 ttggtactat gatcggcatc gctcagtccg gttggtgatt ttggttggtt tggcgattga
     5881 tcagatcgca caatccgggc tgagttccct ttcagtgatc tactattccg cgcagctatt
     5941 tagacacttc cgagccgttg ataatactgg ttttcatatt ctgtcggtga catatgatcg
     6001 ctagaaccat gccgacgctt aggaaggtgc gaacaagtcc ctgatatgag atcatgtttg
     6061 tcatctggag ccatggaaca gggttcatca tgagtcatca acttaccttc gccgacagtg
     6121 aattcagcag taagcgccgt cagaccagaa aagaga
//
