LOCUS       NZ_OP381046             6397 bp    DNA     circular CON 10-JUL-2023
DEFINITION  Klebsiella michiganensis strain K254 plasmid pK254-NR, complete
            sequence.
ACCESSION   NZ_OP381046
VERSION     NZ_OP381046.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Luo,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Department of Clinical Laboratory, Taizhou
            Municipal Hospital, No. 381-1, Zhongshan East Road, Jiaojiang,
            Taizhou, Zhejiang 318000, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.5
            Sequencing Technology             :: nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/10/2023 06:48:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10
            CDSs (total)                      :: 10
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 2 of 2
            Pseudo Genes (incomplete)         :: 0 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP381046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="K254"
                     /db_xref="taxon:1134687"
                     /plasmid="pK254-NR"
                     /geo_loc_name="China: Taizhou"
                     /collection_date="2017"
     gene            join(6281..6397,1..177)
                     /locus_tag="QZJ63_RS00005"
     CDS             join(6281..6397,1..177)
                     /locus_tag="QZJ63_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyl-(dinitrogen reductase) hydrolase"
                     /protein_id="WP_080463864.1"
                     /translation="MTIVISKEIEEKLRTKHQVTNKEVYECLANCKGNLLIDNREQHRT
                     NPPTNWIIAETNRRRELKVCFMIIDGDIHIKSAFEPNEKEQKIYAQHGKKTT"
                     /name="ADP-ribosyl-(dinitrogen reductase) hydrolase"
     gene            369..737
                     /locus_tag="QZJ63_RS00010"
     CDS             369..737
                     /locus_tag="QZJ63_RS00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_073545627.1"
                     /translation="MKTTSEFARDAQKWDDRELGASEDHVEVASMAEMAAFNDALSLQA
                     ISIRLQKNLVSDLKSIAESYGIGYQPMVRDLLQRFVIAEKKQALRRQLDRLNEQEQIHQ
                     QEDTVPVDEFIESIRKQA"
                     /name="hypothetical protein"
     gene            complement(778..966)
                     /locus_tag="QZJ63_RS00015"
     CDS             complement(778..966)
                     /locus_tag="QZJ63_RS00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250649648.1"
                     /translation="MIDKIKAPKGFELLTYFPEDLLGFSLSVFPGVFLLSRWRLSGSPV
                     DSAIRFGPGLRLIVVLL"
                     /name="hypothetical protein"
     gene            complement(1158..1673)
                     /locus_tag="QZJ63_RS00020"
     CDS             complement(1158..1673)
                     /locus_tag="QZJ63_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094955576.1"
                     /translation="MQKVTDLYPPEIAGHKLQDHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKKKYLPVTARTISTKADVGGAVKQAAFCID
                     ESDLARLKSEPEKMMKECERNLDSQKRTNAQKEISRLYKEFGEDGILALLSNVARSNGT
                     PPGAQPAS"
                     /name="hypothetical protein"
     gene            complement(2205..2732)
                     /locus_tag="QZJ63_RS00025"
     CDS             complement(2205..2732)
                     /locus_tag="QZJ63_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094955577.1"
                     /translation="MASTNCITVLTARGIQEILKTGGSQAWRLDASHAAKHQYLVCVQN
                     SKKDWGSQEAKHHHAFMVGQISGVSRAPEHSKRWIINIDSYAEIDIPDQWDGNRNPVSY
                     RNLESMGIDVTRLDFKPVSKVTSSVLNDVKTVDEREENKVFSNPLNIKDAKAGLALYFG
                     VSEDDIQITING"
                     /name="hypothetical protein"
     gene            2860..3025
                     /locus_tag="QZJ63_RS00030"
                     /pseudo=""
     CDS             2860..3025
                     /locus_tag="QZJ63_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009092571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /name="DUF1471 domain-containing protein"
     gene            complement(3868..4290)
                     /locus_tag="QZJ63_RS00035"
     CDS             complement(3868..4290)
                     /locus_tag="QZJ63_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_094955578.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVTYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEYKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4290..4481)
                     /locus_tag="QZJ63_RS00040"
     CDS             complement(4290..4481)
                     /locus_tag="QZJ63_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4897..5009
                     /locus_tag="QZJ63_RS00045"
                     /pseudo=""
     CDS             4897..5009
                     /locus_tag="QZJ63_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(5539..5760)
                     /locus_tag="QZJ63_RS00050"
     CDS             complement(5539..5760)
                     /locus_tag="QZJ63_RS00050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250649649.1"
                     /translation="MARLVTNYSRIKEMESNGLSVEKIAKIFDENAVELQNFVTVADGF
                     VSGEKSLSLDVTNKSKDAFNAVAGALAK"
                     /name="hypothetical protein"
     Typing          4603..5009
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.844"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agggaacaac atcgaacgaa cccaccaact aactggatca tcgctgagac caaccggcgt
       61 agagagctaa aagtgtgttt catgattatc gatggcgata ttcatatcaa gtcagccttc
      121 gaaccgaatg aaaaagaaca aaaaatctat gctcagcatg gaaaaaagac cacatagaaa
      181 aaaaaataag taattgtttt gaaaggtttt acactgtaag acctttcaaa aatcttacat
      241 aggctaataa ttaatattta aaaaagccaa attgaattct taacatagag gttccttacg
      301 gaacaaacga agcatagaaa attgaagcta atcctgatct acaggaaaca gcactagagg
      361 tctacctaat gaaaactacc agtgaattcg cacgtgacgc acagaaatgg gatgatcgag
      421 aactcggcgc ctcagaggac cacgtagaag ttgcatccat ggctgaaatg gcagctttta
      481 atgatgcatt atcactgcaa gctatctcaa ttagattaca aaaaaatctt gtaagtgatt
      541 taaaatcaat agccgaaagc tatggaatag gttatcaacc aatggttaga gaccttctgc
      601 aacgttttgt tattgcagaa aaaaaacagg cattacgcag acaattagat aggttaaatg
      661 aacaagaaca gatccatcaa caagaggaca cagtgcctgt agatgagttc attgaaagta
      721 ttagaaaaca agcgtaaatg acatacatca tcccgataaa gaaatttctt atatatttca
      781 aaggagaaca acgatgagcc ttaagccagg accgaaacga attgccgaat caacagggga
      841 acctgataaa cgccagcgcg acaataaaaa aacaccgggg aacactgata aactgaaacc
      901 cagtaaatcc tcagggaaat aagtcagtaa ctcaaagccc ttaggggctt ttattttatc
      961 aatcaacgcc taaatttatc atcctcccac tgatcgtatc attgcagccg cccaagctcg
     1021 aattcccaaa caggaaacta caccttcttc aaatggaagt cttcgagaga gctaccataa
     1081 caactatgct gcatcgcaca taaaaaagcc cgctttgcgg gcttaggccg gttacttcac
     1141 gatgacctgc aagattatta gcttgcaggt tgtgcgcctg gcggcgtacc gttcgatcgg
     1201 gcaacattgc ttaacagcgc cagtatgccg tcttcaccaa actctttata caacctacta
     1261 atttctttct gagcattggt acgcttctgg ctgtccaagt tacgctcaca ctctttcatc
     1321 atcttttccg gctctgattt aagtcttgca agatcgcttt catcaataca gaaagcggcc
     1381 tgctttaccg caccacctac atcagcctta gtcgaaatcg ttctggccgt aaccggcaga
     1441 tattttttct taagcgaatg agccacggct gacgcacggt taacacacag atcagccgta
     1501 atgttatagc cagaagtggt caccacatta ccgtcacaga gcgctgcaaa agtacacagg
     1561 ctggttttat ttagtttctg gattaactcc agcattacgg tattgccggc gaagtgatcc
     1621 tggagtttat ggccagcaat ttcaggggga taaagatcgg tcactttttg catttatagc
     1681 acctgtttaa ggaggataaa taacaggtga cggtgtcctg atgagccttc gcacatcaaa
     1741 aaactatgaa aaatgcctgg caagtcagta ccaagttact acataatgca gcaccacttg
     1801 acaaacgccc taatcgggcg tttgttgatc aggtaagatt cagttttctc gaactgcgtc
     1861 accggcagct atcagaatcc ttagtttctc aggccgaaga tctgatagct gcctcgtttt
     1921 tactgacaac ctcacgatca tcagaatccc ttattctacc ctattccata agtgcgaaca
     1981 tagttttttt tctgatcttt catgcttgat ttacaaggaa attatctgtc tggtagctta
     2041 aattagcaac aacactacca agtggtacca atgttaaaag catgtttaaa catacagcaa
     2101 aaaagttgac gtaaaaacag ccaactgaat aactcataat cttgctattt accatcacat
     2161 ccgatgtcag aactatgatg ccaagcgtca tcggttttat gatgtcaccc gttaatcgtg
     2221 atctgaatgt catcctcact gacgccaaaa tatagagcca aacccgcctt tgcatcctta
     2281 atattcaatg gattagaaaa aactttattc tcttctcttt catcaacggt ctttacgtca
     2341 ttcaaaacag atgaagttac ttttgaaaca ggcttaaagt ccagtctagt gacgtcaatc
     2401 cccatacttt cgaggtttcg ataagaaact ggatttcggt taccatccca ttgatctggg
     2461 atatcaattt cagcataaga atcgatattt ataatccaac gcttggagtg ttctggggct
     2521 ctgcttactc ctgatatttg accgaccata aacgcatggt gatgctttgc ttcctgagaa
     2581 ccccaatcct ttttcgagtt ttgaacacaa accaaatact gatgctttgc tgcatggctg
     2641 gcatcaagcc tccaagcctg acttcctcct gttttcaata tctcttgaat accccgagct
     2701 gtaagaacag taatacagtt agtacttgcc atttataacc acctttaatc aaaaaaaaaa
     2761 ccaaaaaact acgtaaaaga tagttataac acacaaccac cgacataata aacaaaatac
     2821 taaatcaagt actgattagt acttgattta gtatttacga tatctttcgg tgcatttact
     2881 cagagcgtca ccgcctgtgc atccaccatt gacggagcag aggtccagat cgccgctcag
     2941 gctaaaaaaa gccggtacgt cttacaaaat tactgaatcc aactttaaca acggcgttca
     3001 catgaccgca gagctgacca aataagatgg tcgcagaggg agctgccgaa caggtcgccg
     3061 ggagccggcc cggttcgccc ctcagcggga aaaccttcag gccccttcgg gggctttttt
     3121 gcatctgtca tttaacataa agccttaatt gtacgcaccg tcagaacggc ctcagggcgt
     3181 ttctgacggg aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc
     3241 aggctctgtt tttacggcat gcatgcgtat gcacgcaagt gcatagcgcg aaaactcacg
     3301 ccattcagcc agggtaagtc ctgtttccgg cgttctttcc atccgaaaac ggtgaacggg
     3361 gacgcaattt attgcgtggc cgtgaagcgt tgccggcgca gccggaaaac cggttttcag
     3421 cacgttcagg aatcgtgttc gtcgacggcc ggcaatcacg ttatggcaag tcgccggacg
     3481 actcaccaca cgattcaccg gcgactcttc actagagtcg tacccagagt cgccgcgtct
     3541 aaaaaacaac caaacgggga gtcgggtcta gagtcgggtc gttattaact cattgttttt
     3601 atagtcctga gtcgttaccg actctatgac gactcgccgg ggactctatg acgagtcgtc
     3661 atagagtcgg atctatgtaa ataaaattta aaaaacgaaa tttcacgaaa gtaaacaact
     3721 gatattaaat acaaatctgc aaaattttaa ttttgcgtag tgtgtgggta tttttagtcg
     3781 tgaggagcgt tcagagatct ggcgattttt gggaaggttc aggaactccc cgggatggcc
     3841 ggagaggaaa actgaagcat gacggggtca ggattttttc cagaatttcc accaggggga
     3901 atggggtggt gaagaaggtt cgtgcttgta ctccagaagc tgcatagcct gacgaaggga
     3961 atcaatatgc tcatctcgag ccttaacagc ccctttaaga ccatcgtttt cagcttctag
     4021 taaagcaatt ctatgttgta aatcatgtaa accaacgctg ttaacacctg tagattctgc
     4081 gtcacttttt acatcatatg ttacagtatc gatgacacca tcaagagaac caaacactct
     4141 aataagctct gaggtatcaa taactttaat agccttcccg tgctttacaa cagaggtgta
     4201 acttaacttc ccactgttaa tcatcttgta aattgtgggg cgactaacct taacaagccg
     4261 tgccgcttca ctaatagaca ctctggccat cagttaccat cctgtaatgc atccagtctc
     4321 gaggacagtg tacggaaaag atgctccgca tcatcgtgga gtttctcaca aaggtcggcc
     4381 tcgacatcaa gatcaagctc gttaagcttc tccagcagga gcagagactg attctggatg
     4441 aatttagcca tgttcagcgc tgttttctgt tgtttcgtca taagaagcct cagagcgtca
     4501 gtgctcaatg tagggatgtt ctcttttccg cttcctcgct cactcagtcg ctacgctcct
     4561 gccgttcgac tgcggcgagc ggcaggagca tgcgtataat agctcgagaa aaccgagcgg
     4621 tcgcgtgacg gggaaaagga aaggccgcac ggcggccgtt tttccatagg cttcgccccc
     4681 ctgacaagca tcacagaaac tgacactcaa atcagtggtg gcgaaacccg acaggactat
     4741 aaagatacca ggcgtttccc cctgatggct ccctcgtgcg ctctcctgtt cctgcctttc
     4801 ggtttaccgg tgtcattccg ctgttatggc cgcgtttatc tcattccacg cctgacactc
     4861 agttccggga aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     4921 gactgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaga cacgcaaaag
     4981 cgccactggc agcagccact ggtaactgat ttagaggagt tagtcttgaa gttatgcgcc
     5041 ggttaaggct aaactaaacg aacagttttg gtgactgtgc tcctctaata agccagttac
     5101 ctcggttcag aaacttgccg gttaatgaac cttcgaaaaa accaccttgc cgggtggttt
     5161 tttcgtttac agagcaagag attacgactc attgaaaagg atctcaagaa gatcctttgg
     5221 tttaccatcc ccctgacaaa ttcgaccgta gatgaatgtc aacgacggac aaaaagtacc
     5281 ccaccttagt ttactcagga tgaccgagct cttttcgtaa tctttgttga tatctattgg
     5341 ccagagcccc catacagggg ggagtttgga gatcggaaat tattgtacgt ttagggcgtt
     5401 atttaaccaa tgtaggtaaa cgcgttcaga acccaatgtc gggcggttta ttgttctgtc
     5461 tgtgatgcgc gtcgtgagct ggcgcgtcgt tcctgactat gaaaaagccc gcttagcgcg
     5521 ggctttagtt tatccgtttt attttgctaa ggcaccggcc acagcgttaa aagcatcttt
     5581 actcttgttt gttacatcca gagaaagaga cttttcaccg gaaacaaaac catcagcgac
     5641 ggttacgaaa ttctggagtt cgacggcgtt ttcgtcaaaa attttagcaa ttttttcgac
     5701 gctaagaccg ttagactcca tttctttgat ccgagaataa ttagtgacaa gtctggccat
     5761 gcctacgggg ttagtcagaa cacgggttac tgtactcatg ttttaagttc cttatatgaa
     5821 ctgggagacc agtgaaagac cctttccata tgaggatgtt ttattcaatt tcaagggggg
     5881 gggactccag atctgctagc cttttttggt atatcccctc gacgatttcc ttcccgtggg
     5941 gaagtttcga tatagcaata gagtgtactc ctgcgctata cggagttctc aggggacttg
     6001 accttaatcg gataacctaa atgcgatagc ggtagccgct gacgaaaaat caatccgtta
     6061 cgcatctcat ctggtcaaat aataacctaa atgtacagta acttacgatt tccaaactga
     6121 gcccatacat cccaaaggga ttaagtcagt tgaaaaacac atgctgtata tacacataag
     6181 cagccaaaaa tagcgataaa gctggccagg cattgactaa tgtaagtttt gtatatacaa
     6241 taagttcaat ttcacaacga atgccgaggc gccatgcaac atgacaattg taataagtaa
     6301 agaaatcgaa gaaaaactaa gaactaaaca tcaagtaacg aataaagaag tgtatgaatg
     6361 cctcgctaat tgtaagggta acttgttaat agataac
//
