LOCUS       NZ_OP378682             4074 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Enterococcus faecium strain VRE33085 plasmid pVRE33085_8, complete
            sequence.
ACCESSION   NZ_OP378682
VERSION     NZ_OP378682.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Empirical genomic methods for tracking plasmid spread among
            healthcare-associated bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2022) Medicine/Infectious Diseases, University of
            Pittsburgh, 3550 Terrace Street, Scaife Hall S853, Pittsburgh, PA
            15213, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Sequencing Technology        :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028738505.1-RS_2024_03_26
            Annotation Date              :: 03/26/2024 01:34:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 5
            CDSs (total)                 :: 5
            Genes (coding)               :: 5
            CDSs (with protein)          :: 5
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP378682.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VRE33085"
                     /isolation_source="Clinical"
                     /db_xref="taxon:1352"
                     /plasmid="pVRE33085_8"
     gene            complement(334..1749)
                     /locus_tag="PWB76_RS00005"
     CDS             complement(334..1749)
                     /locus_tag="PWB76_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002303405.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREKNEREK
                     ALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADFVDLYQK
                     ASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSAVVAENKK
                     LKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKTLASQQNAP
                     ERNIGRSENEMEW"
                     /name="plasmid recombination protein"
     gene            complement(1971..2333)
                     /locus_tag="PWB76_RS00010"
     CDS             complement(1971..2333)
                     /locus_tag="PWB76_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303404.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKKSQ
                     MSESKFTSMFNDVSLR"
                     /name="hypothetical protein"
     gene            complement(2337..2684)
                     /locus_tag="PWB76_RS00015"
     CDS             complement(2337..2684)
                     /locus_tag="PWB76_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171346404.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLNLPDEDNYSE
                     MLERMMDVLNSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQIGV
                     IYRNKSKGKEL"
                     /name="hypothetical protein"
     gene            complement(2755..2988)
                     /locus_tag="PWB76_RS00020"
     CDS             complement(2755..2988)
                     /locus_tag="PWB76_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303408.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
                     /name="hypothetical protein"
     gene            complement(2985..3959)
                     /locus_tag="PWB76_RS00025"
     CDS             complement(2985..3959)
                     /locus_tag="PWB76_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002303407.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVGN
                     LKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQLRRG
                     KATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYKYRNLL
                     KQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(2986..3881)
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(338..1842)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.207"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtataatga atttgagtta cggagctagt gtgattagtt tccgtaaagg tcgagaacgt
       61 gtccagcgtt gctcgacttt atttttttgc tcttttttca gtttatttat ttgataactc
      121 aataatagca cttctcttta gctatgcaaa tatccaaaaa cataaaaccg caccctttga
      181 aaagggcgcc cccaaacttc gctaacgcta agttggccaa aaaatggact tgccattttt
      241 tagcaaatgt cagcttcagg gattagtggt ttagccgtca actccttatc ctcggcacag
      301 accgcctccg gtaccgttga ctgcaccgta taactaccac tccatctcat tctcagaacg
      361 ccctatatta cgttctgggg cattctgctg actagccaac gttttctctt gatctaatat
      421 ttttagctct tcagatattc ccatatcttt ttctacaagc ccaaaacgaa ttgataaacg
      481 agtgattgat ttttgaaatg cgtttttcag ttgagttaac cgccgacgca aagaacgatt
      541 ttcttccttc agctttttgt tttcagcaac aaccgctgac acatcaacct tttgttgatt
      601 tttcaaaaaa gaattttctt gttctaacgc catgttttta tcaaataaag cgatattttt
      661 atctaatgta tttccaagcg attcattcgc catatcagca tattttttgc tgttcgtagc
      721 attagacgct ttttgataca aatctacaaa atcagcacta gataagcgat aagaaccatc
      781 tgataatttt tcaaaactat tgagtggcac aggatctttt tgaaatacaa cagccagctc
      841 tttttccaaa tcgataatcg attttttctc ttcttcaatt ttcgccttct cagaagccac
      901 cagagccttc tcacgctcgt ttttttctct ttgcttagaa atatacctca tttccgcaga
      961 aatgctctca cgctctttta cagccgtttg gcgcaattca gaaatttcac tcatgatttc
     1021 tttgtattcg tgaatatctt taattttgtt ggtaccgaca atatcccttt tccaaccgta
     1081 atcattcatt aatcgttcta tagagtcaac ttcttgattt ctaaaattat tgaaaagcac
     1141 tcgaccatta tctttatcag cgtgtatccc ttgttgaacc agtgctttat taaagctcgg
     1201 ctgcttttga acaccccgtt tataaccttc agcaaccggc acaatattca agtgcaggtg
     1261 cggacttatt tcgtcattat gaataacagc attataaaca gccaaattag ggtttctctc
     1321 ttgaaactcc ttgtaatact gaaccaaaat tttattggca atcactcgat tttgttcagc
     1381 ttgttgtttc gatatgccag tagaagaacc atctgctcga tttgtctgga aatcatctaa
     1441 aatgcctatt tgcacaataa attctcgttg aggttccaac tttttatcct tgcgcacttt
     1501 cgctaaatag tcctcgattt ttctatcggc tcttttttgt ttcgcattat atttttctac
     1561 cacttcgcca aacaattcct catatttatc tgctagattc gtctgctcta aataaatatt
     1621 ttcatccgat ttcgtgaagt caatatgctt atgccaatca ttggttttct ggctatcagt
     1681 taactcacga ttattatgag aaatacttgt attcttcgtt ccttttttaa atgaaatact
     1741 aaaccccaag ttaaaaccct ccttcgcgct gtcgctaata aaaagagtgt aaacctttct
     1801 gttactcttt gtcaaagagt aacattttcc taattgtgac atggctgtca caattccaaa
     1861 tgctctccga gggtattcgc tgtcacaagt gacaacgaaa aaaggagccg aaagcgtgtc
     1921 acgctctcaa ctccttttac gctttatttt gcttcacgca aaataatcgc ttatcgtaaa
     1981 ctcacgtcat taaacattga cgtaaattta ctctcactca tctgactttt tttactcggt
     2041 atcactccga gtaagccgtc agattcattg ggataatctc gttcgcttgg cgctgatttt
     2101 tcatcagcaa gccactcatt atccactgat ttttcgtgtt gctgatcgtc agaaattgac
     2161 gataaccacc gctcaaaatc atcaaaagat tcaaatccaa aatgcttccg aagtgcctta
     2221 cccatttcag cattttcctt ctcaatcaat cgttttttct gttgacgaag ttttgcctgt
     2281 tcttttttca atttttcttc ttgtgcttta atatctgcta aagtcattcc caagatttac
     2341 agctccttgc cttttgattt atttcgataa ataacaccaa tttgatttaa ttgcataaca
     2401 taagttaaaa tattcagcca aaattccttt tcagattcat cttctgtttc ggctatttta
     2461 ccgtaaacaa atttatcagt aatcaaagaa gaatttgcta ggcctacatt gattgtagaa
     2521 ttgagaacgt ccatcattct ctcaagcatt tcactataat tatcttcatc cggcaaattc
     2581 aaataatcag ctaatcgttc atacatcaat tcaattctat caactgtctc ttctcttaaa
     2641 tcaattttat ccatgttaca cctcatttcc aaaactgcca ccatttccga ttctttaatt
     2701 ttcgatacac ctaattcatc agcaagattt tttatggtca tcttttctga caaatcagtc
     2761 attagcttct ccactacttt cagctaccgc ataaatatat ttaacggcgt gcttagggat
     2821 aataatcgga gagtcaattt tattattttt caaaatcaat tcatattgcg ttacatcaag
     2881 taacttaccg gacatttttt tgctatccaa aaaaacaacc acaacgtaat cacctgttaa
     2941 atttaccaac caatttttct taccaaaata cttttgtttt tctgtcattt ttcttcctcc
     3001 tctgttacgt ctagccctag aagccatgtg tctaattctt tttttaaatc gtctgccgat
     3061 tctgcaaagg tttctcgtaa atgattcgtc aattcatcat tttgactttc ttgcttcagc
     3121 aaatttcgat atttatattt tgtctttcga ttaatttgcc cccaataatc atgttctgtt
     3181 gttaaagcag taatcatcat tttgtcagct acacttgtat cagcaggcaa gtaatgagga
     3241 ctagcaaaac tatctaagct ttcataaacc atagcatgcc aatcagtcgc tttccctcgt
     3301 cttaattgta attctaaccg ccaccaactc acgatttctt ttggcacaat ttttcttttc
     3361 ttctcttgtt ccagtttttt attatacatc cgaatttgac gttcagaaga gcgagatccc
     3421 caatatgccg tctctaattt tccattccgt ccataaatag gcttaaaaga aactggatca
     3481 actacccgat attgtgtaat aaactcatca ggtacatcga taatgtcgca agcaatatct
     3541 gcacgtgaaa agtgaggttc gataaacaaa tcatgaataa aatttttcat ggaactagct
     3601 aagaattgac taatcttatt cggattgaag tctatacgcc ctttcccctc ttgccatttt
     3661 aaaatttcaa tataagcaat attctcttca tgttggtcta acaactgcca accattatta
     3721 ctaacagatc ttaaataacc gttaccttca tttaggcgca taagctgttc aaaatctaaa
     3781 atcaacacat catgtgtagt gtgataatag atattctctt taagatttcc aacaatggta
     3841 attcggtcga ttgaccactg aatttttagg ggggtgtcca taaaagggac acttaaccgc
     3901 ctgttagagg gggctgttaa tttttcccac tcatttatga tcgtgccttt tttactcaaa
     3961 tttagttcct cctcaaagaa aaaagagatt ggtggaaaga attttccaga gttcacgtaa
     4021 ctctaaaaaa ttcataacca gcaatctcta gtcttgaaga aacctgaaat tatt
//
