LOCUS       NZ_OP378676             3199 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Enterococcus faecium strain VRE32643 plasmid pVRE32643_5, complete
            sequence.
ACCESSION   NZ_OP378676
VERSION     NZ_OP378676.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Empirical genomic methods for tracking plasmid spread among
            healthcare-associated bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2022) Medicine/Infectious Diseases, University of
            Pittsburgh, 3550 Terrace Street, Scaife Hall S853, Pittsburgh, PA
            15213, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Sequencing Technology        :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028738445.1-RS_2024_03_26
            Annotation Date              :: 03/26/2024 01:34:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP378676.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VRE32643"
                     /isolation_source="Clinical"
                     /db_xref="taxon:1352"
                     /plasmid="pVRE32643_5"
     gene            280..525
                     /locus_tag="PWB87_RS00005"
     CDS             280..525
                     /locus_tag="PWB87_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002311817.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMT
                     PERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            1575..2294
                     /locus_tag="PWB87_RS00010"
     CDS             1575..2294
                     /locus_tag="PWB87_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            complement(2431..2847)
                     /locus_tag="PWB87_RS00015"
     CDS             complement(2431..2847)
                     /locus_tag="PWB87_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2850..3134)
                     /locus_tag="PWB87_RS00020"
     CDS             complement(2850..3134)
                     /locus_tag="PWB87_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     Typing          1576..2294
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgttttttgc caaaatgcta tacataagct acccacgatg gcgaaaaagt gatatcaaat
       61 ttttttctga gaaacgtgat aggcacagcc tatctaccca ctttttttag aaagtgggta
      121 cacgtttcct tatgctcttt aaaattcggg ggggagtgct gcacgtgtaa tcgataacca
      181 aatttaaaaa tgcacgatca aaaatttatt tagtttttca aacgacattt tttttgagag
      241 tatagacggc aaagaagtga ttttatttct agaaaaattg tgagactgga gaaagaaaaa
      301 atggaaaaat ttccttacga atattttcaa aacttagaaa aattagagca agcaattcaa
      361 gcaatcaagt tcgatttaac agacgaaaaa ttgttggaat ataaaaatat gacacccgaa
      421 agaaaacgat cgttggaaaa attaatgcaa ctagtgaacg aagacggcgg aatttcagac
      481 gcagcttttg acgcatggag cgaatttcat ggagaatata attaagtggg tttaaatggc
      541 tctaaaatcg cttctaagag ttttttagct tggaccaatg aaatacacca aaaactaaat
      601 aaaacgtctt aaatcgaaaa atacgagctc tcagagcgac tttatttgta aatgttgcat
      661 ataagaaata attacggatt ttagactttt gtaggatatt tttgaaataa atatttattt
      721 ctgttataat ttgattgcaa ataagtgaac acgaaacaca cgaaagcccc agagaggtcg
      781 gattctctgg ggcttttttt gtgggctgag tcgccttaat taagttttac ttcttgtttc
      841 ggttattcaa ccagtaactg aacaaagcgc ttaaaagccc aacaagaagg gagcaaataa
      901 agttagtgaa cacgaaacac acctccttcc agtcgcaaga ctgtcggata ggcgacaatc
      961 aattatacaa taaaaaagca aaaataagcc taagtttttt attgccttgg ggttgttagg
     1021 gcttgccctg accgcctgta aggcgcttta gaacgaagga catagttttt atcgaatctt
     1081 gtcttagaac ggcttagacg agctctcagg acgccacaaa ggagtcccta atgaattatt
     1141 tttggcgaag gagcctaaag gcgactgctg caagtaaaat gtgagagcgt agcgatcact
     1201 ccttcttaca gcgaccatag ggagctagat tggcagttta atatcatgcg cgaacctgtt
     1261 tattttaaaa agtgggtgat tttttggcgg gaaattttgc tcgcgcgcgc gcgtttattt
     1321 tatttatttt ttaaagattt tttagaaccc tttttagggg gcagttgagc cttactctcc
     1381 caacggatac agaagggtac taacaacttt ttatctgagt actaacaact ttttatctga
     1441 gtactaacaa ctttttatct gagtactaac aactttttat ctgagtacta atatcctttt
     1501 gtataataac aaccaaccta tttacgagtt ggttgttaaa ggttattatt tatttgtaag
     1561 gggggaagaa aggaatggat gaaaaaacgg aattaacagt caaatatcaa aatgaattga
     1621 atttagtacc gctgaaaaat ttcaatgcta agcagatgga tttatttttt gcattatgcg
     1681 ctcgtatgaa agatcgtggt ttgaaaaaag ttagattttc tttcgaggaa ttaaaagagt
     1741 taagtgatta taagatgact gctacaaaag cctttgtcgg tgatttagaa aagctctata
     1801 aagatatgct taatcttact taccgtactg aagatgagga ttctattcgc tactttgttt
     1861 tgtttactgg ttttgaaatc aataaaacaa aaaagtttgt tgaagtcagt gttaatccgg
     1921 acctagaata tattattaat ggtttaacag ctgaatttag tagatttgaa ttatctgctt
     1981 tcacgtcgtt gcgttctacg tatgcaaaaa ctctattccg actgcttatg cagtaccgtt
     2041 ctactggtta ttatgtagtt cgtattgaag agtttaggag cttaatggat atcccagaat
     2101 actatcaaat gggtaacatt gatcaaaaag tattaaatcc tgcattacga gaactaaaaa
     2161 aatacttcaa tgatcttaaa gtcacaaaaa taaaagcaaa aaagggcaat aaaatcgcca
     2221 aattagaatt tactttttca agcctaacgt cagatttgcc aaagatttct ctgcgtaact
     2281 ggttagaaga ataaataaaa aagaactcgc cccccttaca ttaagcaagt agacaagtcc
     2341 cttacaaccg actaagattg ctagtacatt ctagcatatt gtatggggct tctaaataaa
     2401 aaacagatag gaataactcc tatctgtttt ttatcgtttt agaaaatttg ttgggtcgaa
     2461 atttctagaa gtgactgttt gactggtttc tttttgatga ttctttggtc gatttcgatt
     2521 tgatggattt agtttagctt cgattttttc tttcacttct tgatatactt tagaatttgt
     2581 gccaataaga acgcctgtat cttttagata taaatcagca aattctgcga tgatttcagt
     2641 catgatggaa atttcttttc ccatagcatt tagttttgcc tgattgctgg tagtggtcgt
     2701 gttggtatcg ggttcataac tcatacataa aaagcgaatg gcttccgagt agttattgat
     2761 atctggattt ttttcgatga tggaatctaa aacgtcaaca tgactagggt acaaagtaac
     2821 atgtttttgg acagaagttt ttctcatggt tatatcattc ctccgataag catttcaatg
     2881 atttgattat ttgtgtagac atactcgctt aatatttgag tattttctgt ttggatagca
     2941 ataaatttat cgagcaataa atcgaattgg tcttttttgt aagtcgtaag ttgacggtca
     3001 aaatcacttt cgattaaaac ttttagatat ttatttcgac tccatttttc tcctgtttta
     3061 ttcgtgagtt cttggcaacg tttgtctatt gttgcaacaa actttggatc aatattccga
     3121 actaaaatgt ccataaaaaa tctccttttg caattatgta taatttatgt ataccataca
     3181 aatactatat ttgtatagc
//
