LOCUS       NZ_OP378673             9317 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Enterococcus faecium strain VRE32553 plasmid pVRE32553_4, complete
            sequence.
ACCESSION   NZ_OP378673
VERSION     NZ_OP378673.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Empirical genomic methods for tracking plasmid spread among
            healthcare-associated bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9317)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2022) Medicine/Infectious Diseases, University of
            Pittsburgh, 3550 Terrace Street, Scaife Hall S853, Pittsburgh, PA
            15213, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Sequencing Technology        :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028738415.1-RS_2024_03_26
            Annotation Date              :: 03/26/2024 01:38:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 13
            CDSs (total)                 :: 13
            Genes (coding)               :: 13
            CDSs (with protein)          :: 13
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP378673.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VRE32553"
                     /isolation_source="Clinical"
                     /db_xref="taxon:1352"
                     /plasmid="pVRE32553_4"
     gene            complement(join(9156..9317,1..576))
                     /locus_tag="PWB80_RS00005"
     CDS             complement(join(9156..9317,1..576))
                     /locus_tag="PWB80_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            1221..1307
                     /locus_tag="PWB80_RS00010"
     CDS             1221..1307
                     /locus_tag="PWB80_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1428..2117)
                     /locus_tag="PWB80_RS00015"
     CDS             complement(1428..2117)
                     /locus_tag="PWB80_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(2119..2397)
                     /locus_tag="PWB80_RS00065"
     CDS             complement(2119..2397)
                     /locus_tag="PWB80_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(2357..3271)
                     /locus_tag="PWB80_RS00020"
     CDS             complement(2357..3271)
                     /locus_tag="PWB80_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3253..3633)
                     /gene="mobC"
                     /locus_tag="PWB80_RS00025"
     CDS             complement(3253..3633)
                     /gene="mobC"
                     /locus_tag="PWB80_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(3954..4115)
                     /locus_tag="PWB80_RS00030"
     CDS             complement(3954..4115)
                     /locus_tag="PWB80_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305245.1"
                     /translation="MKLNQLKNGRVIARFLVEVTKSGHIIIMTRRKSDKKLAKNIAECL
                     IKLSVLAK"
                     /name="hypothetical protein"
     gene            complement(4326..4946)
                     /locus_tag="PWB80_RS00035"
     CDS             complement(4326..4946)
                     /locus_tag="PWB80_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(4943..7069)
                     /locus_tag="PWB80_RS00040"
     CDS             complement(4943..7069)
                     /locus_tag="PWB80_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            complement(7123..7497)
                     /locus_tag="PWB80_RS00045"
     CDS             complement(7123..7497)
                     /locus_tag="PWB80_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            7630..8091
                     /locus_tag="PWB80_RS00050"
     CDS             7630..8091
                     /locus_tag="PWB80_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(8237..8359)
                     /locus_tag="PWB80_RS00055"
     CDS             complement(8237..8359)
                     /locus_tag="PWB80_RS00055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323537.1"
                     /translation="MFILEKARAYEALPKIYHILQKITSLKKMTDKNAQRIRYR"
                     /name="hypothetical protein"
     gene            complement(8589..9128)
                     /locus_tag="PWB80_RS00060"
     CDS             complement(8589..9128)
                     /locus_tag="PWB80_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     BGC             2123..9317
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2..576)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2361..3271)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcttgggca aaaatttgtt tatcaatatc tctcatgcga taactttctg gtattgctaa
       61 tagagctcta aaatcttcta tttttacaaa ccagtgacct tttttagatc ttctgtcttt
      121 atgagccata agttggcgat ataactcttt aacgtagcta ctctttaagt ttgtcatatt
      181 ttctaattcg aaacgagtaa aattagttcc gattgaatta agtatatatt caaattcttc
      241 agaaaccttt attaataatg tttcattcac tttatcgatt ttaaattttt gaaacagatt
      301 taattgttca tatgtttttt cgtcttcata aataaaagac aaagtaatga ttttttttga
      361 catttttatt aaatcattag caaaacggtc gccacgtgca tttatattgt aatcaattaa
      421 ttcttttatt tgtttaaatg taaaaatttc ttcttttgta ccttttcttt tcattttaga
      481 acatattccc caaaataaat ccatatctat aggggtaaat tttcttaaag ggacagaatt
      541 taatccatta ttatatcgaa ctatatcgtt actcaataaa aatcacctca ataaattatg
      601 ttatcataga cataagagga tgtaaaacac aatttatccc ccatagtgtg acaatttatc
      661 ccccatagtg tgataattta tcccccatag tgtgacaatt tatcccccat agtgtgacaa
      721 tttatccccc actacctatg tcaatactta ctctcccata aggttgctaa tccctaacag
      781 ttattattaa cagtatatct aacagtatat ctaacagtat atcttaagag cacattgaat
      841 ttcccgccta aaaaactcct actttttcct ccaaaaaggt tcgcgaattt ttctaaactg
      901 gaaaacttgc tccctacggt cgctaaaaag gtgtgatcgc tatgctctca tattatactg
      961 cgcagtcgcc tccggctcct ttgctaaaac cgttgttcaa agaggaggtg tgagactctg
     1021 aaagcttgtc taagccgttc taagacaatt cttgattaat ttcccttaat ctagtcgaga
     1081 agcgccttac aggcggtcag ggcaagccct aacaacccta aaacaagtga tacttaaaaa
     1141 caccacacaa atttaatttt ttaagatata ataacttgtc gcctatccga cagtcttgcg
     1201 actggaagga ggtgtatcta atgtttgaac tgttcatttg cccccttctt gtaggagttt
     1261 gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat caataaaggc
     1321 gacatcaacc tgaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtatcta
     1381 atgtttgatc atttgcacat tcagtttaac atgattttaa atcaaattca attttgttgt
     1441 tgctgatcta accattcctt gacacttttt agatagttta aggcggtttc tttttttgtc
     1501 ggattttgca tgttagttgc ttgttgaagt aagatttcat agcgttcata tgtgacagat
     1561 tgttgttttt cttttttttc ttcttcactg gcaaaccaag ggtttgtgtc tccaaataga
     1621 tcactacagg cttcttctat ctttgtatct tttacatatt gcacgtcttt tttggttcgc
     1681 tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata aagacgagtg
     1741 agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt
     1801 tcctcataac aagtggtaaa ctcttccatt ttttgtgcta atgcttgctc ttttttcttt
     1861 ttatcacgcg ctgattcata cttttgagtg acatttccta ataaaatact tctagaaacg
     1921 cgcttatttc cctctaaaac gccgaataag ccgcttgaaa cgattatcaa ggaaaatata
     1981 cagccaagaa agaaaaatcg cttaaatcga attttagagc cttttaatga atatcttgtt
     2041 tgaaaaattt tatagatgag ccaaatactt cctgaaatac taacaaaaat gagtagatag
     2101 ggtaaataaa atgccatttt agcctcccca tactaaccaa aatgcgaaca gtgtcaggac
     2161 taagcaacat aagggggcaa gaaaatccaa tgatcgttga ataagcacct ttttctgttc
     2221 cttatttcgc tggtctaata gctcttgatg gagtttttta acttgtaaat caatcgtagt
     2281 ttgtatcctt tcttgcgtat aaggaacgag aaaagcacgt tgtttcttta tttcgttttc
     2341 tatgtctttt aacgttttag agcgataatt cttgttggtg gtctgttcct ctttcaacgt
     2401 gttgttcatg gtttcttcga gccggtctaa tttccggttg ttcaggcgta agagttcgtt
     2461 gttctcttcg tacttcttct gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt
     2521 tggttcgctg ttggttatag gttcgttctc gtctgtccag ctcatgtaaa atcgtctcct
     2581 tttcgtagtc ctttcctagt ctgttggatc gtactttttt ctttttgtgg atcaattcgt
     2641 aagtgaaatt tttcccgcga ttgaagcaat gaatccccaa tgttttgagg gaattacaaa
     2701 attggtcata ggttctggtt tttttgtttt tcattgtttg atcgatcgca atccgaattt
     2761 cgtctttcca aacaggttca tttttttctt ttaattttat ttcagacatg gttcgttttt
     2821 ctggtttctt tcctcgctca gtgattggta agttatgtgc aagacaaatc tcgtcattta
     2881 ggtcataaca tttgtttaga aatgcttgac catgagcttg aaattttttt ccagtctcca
     2941 gattaacgga gttaatgaca atgtgattat gaatatggtc tttgtctgta tgtgtgtaaa
     3001 tagctatttg gtgatcgggg gctatttttt ttgcatactc tgcccctagt tgattagctt
     3061 tttcagcggt aatttctcct ggtcgaaaag attgaatcaa taagtgtgcc tgtacacggt
     3121 cgtcttttcc atacagcatg cgaatttgtt tcatttcact tttggcataa tcgacgtcac
     3181 agtttattcc tgttttaatg gttgctcgtg gttctgcgta attaattgca cgactacaag
     3241 aagtggttcg gcttacctta attgttgcca tatcttttgt agctcctttt gcatgatttt
     3301 taggttttcg gataaacgtt gagcgtcacc agaagcaacg tcatgttggt gtgtattgca
     3361 ccacttagca atttgattga tattccctcc agctttggct aaatcgcgcg cgattgcctg
     3421 ggcaatgtct ggcgccactt tcggtgtcac cagacgcgcg ttttgtgcct tggctttcac
     3481 gaaagccggc acactcattt gcaaagcttc agccgattgc ttcagctttg caaattcgga
     3541 ttcgctcact cgaaaactga tttgttttgg ctcggatcga taggtatatt ttttagaaga
     3601 attgctagcc aaattttgat tttgttcgct cataagtccc accctttcag tacgtcctac
     3661 ggacgtatcg ttcgcaatac cattgtacct aaaaaggtac aatgagtaaa gctcactaaa
     3721 agggtttggc aagctctata tttgctagcc aaatacctac gtatttagtg caaataaccg
     3781 cttgtcaaag gggcgcaagc tcccccttga atccccctca aaagccacgc tccacaatac
     3841 aaaaaaagct agcaaatgct agccactttt tgtaaagttt cgcttatttc accgaagatg
     3901 aaattgttgc tttgcaacaa aaaacataaa tgcttttttg tgtaaactac taactattta
     3961 gctaaaactg atagttttat taaacattca gcaatatttt tagccaattt tttatcagac
     4021 tttcggcgag tcattatgat aatatgtcca ctttttgtta cttctaccaa aaatctagca
     4081 ataactctcc cattcttcag ttgatttagt ttcataagtc ttttgtaatt gacggtttaa
     4141 tttttttggc aattgcaaag aataaataag tctatcataa aatttttcat actctttttt
     4201 caataaaatc acttgggtgc ctccttttta ttaagtgatt atttaaattt tgggatagga
     4261 gagatggaat ataaaataaa tacccgtggt gctagtttta aatcagctag catcacgggt
     4321 aaaaattata attctataat attagttcca tagtttagaa catttaagtc atgtgttact
     4381 agtacaacgg cttttttttc tttttctgtt aaatttttca gaaagttcat tataaaagtt
     4441 gagttttctt tatctaaatt tcctgtaggt tcatctgcaa ataatatatc aaatggtttt
     4501 aacatatttc taattaatgc tattctttgt tgttctccac cgcttaagag aaagattttt
     4561 tcttttaaga tatcttttgg caaagaaaaa tctgttaata aattataaat atgatcattg
     4621 gaaatattac tgttaaactt ttttgctaat tcgaaattgt attgtactgt ttgattttca
     4681 agaagagcat agttttgaaa taaatagcta atatgatttc ttcgtagacg ttgtatatca
     4741 tgtttagaaa ctttcttttc aaaatagaat aagttaccgg tatattgttt atctattagg
     4801 cttaaattat ttaacagagt agtttttcca ctaccacttt ttcctgttac tataataaaa
     4861 tctttttctg atattgaaaa gttccaattt tgtttgatct tttttttatt aattgacagg
     4921 gtgaaattct caactgataa tatcataaaa tattctcctt aaaatattta aaactatttt
     4981 ttttataaat aataatagaa gtaattagat ttattaaaaa gatcataaaa attataaaat
     5041 acaagtaagt gttttttgaa aatatcaatt gaaaacacat agtagcaaat aactctattg
     5101 taaaagggag taaacataaa tctaaaactt gacttattgg tgaatatcca aatatttttt
     5161 ttaaagctat tttttttgaa tttatagtaa aactaacaaa aatcatttga aaattaatta
     5221 ttataaataa tcctaatatt aaaagatatt gtagatatgc atttaattta tttacttttt
     5281 tttgagataa taattcacca gtttcttgaa aaacagaata agaaaaattt atgatatttt
     5341 ctaggttaaa ttcttttata tacggattaa tttgattaaa aaagttttgg tttgaagtat
     5401 gtttctgatc aaaaaataat ccaccatcac tagtcaatat atttccccaa aataatgatt
     5461 ctgttatttc tggattaata actctaacta tgggatcagt tattgtatag ttatcttttg
     5521 cctttccata taaggtatta aaactaaaat acttttggtt attattaaca aatattagtt
     5581 ttatttttaa atttttcaaa ttaggatttt tataaggagc ttgaggagga gagtcctcaa
     5641 gtctaaactg tatgtcttct aaataatttt tttcaatcat ttttgcatat tctttatatt
     5701 ttttaggtac tagaatgtaa gtagttagtg gatcagagtt tattaaattt aagtcagtag
     5761 attcttcaaa agaaatagga ttgtaatatt ttagatagtt taaatcaaca catattcctg
     5821 caccagcagg ttgactaaca tagtcttcaa aatttggata aactttttgt ccaataaaaa
     5881 attctttatt attctcttgg atagttgcgt aattgtatgg cgcgataata aaagttttat
     5941 aattcttctg tattttataa acaaatttac tagctttttt caagtagtta ttttcttcta
     6001 cggtgttgtt tctatttaat tgattagtaa tatttgtttt gtataaatta gtagttcttt
     6061 cccaatattt aagagaatta ttttcggctt cattatgggt atttattact tttgtagaag
     6121 ctattaacag aaggatcatg cttaagaata gacagatatt taatatacaa agatttaact
     6181 taagtggtcg tgaatttttt agataatttt ttatactaga taaatgatag atacttagag
     6241 taactatgat aaataaaaaa ctaattataa aatgaaaaat actacataga agaaatgata
     6301 ggaaaaaatt ttcgttactg taatatatgc tcaaaaaaat agataaaatg aaagttataa
     6361 tcaaagaaca aaataagagg tttaactctt taatagaatc ttttatagta tcaaaagagt
     6421 tatagcctaa catttttttt agagtgattt ctttcatttt tgaaaaagca aatataattg
     6481 tagtaattac aataaatata ctaagatagc agataacaag aacaatatta gtattaattt
     6541 gaaaagtatc tgattgattg atattggaaa tagtaggtgt tccatatttt ttcagtacag
     6601 gaattaattt gtttgagttt ccttgtacat ataatatttc acttaatcca atattttcca
     6661 catgagaaaa aggataaata tttatattta aaaaattatt tggaagtttt aattggtact
     6721 tagaattttt gttattagta taatggactt tagaatcgtt tcttgctaat atattttttt
     6781 tagaaaattt ccagtgtttg ttagcgttag ggtttgtagc aagaatagtt agttggttaa
     6841 cagcagtaaa actatattga gaaatattaa tattttcttg tgtagatatt tcatacaaag
     6901 cgtttatata actgttaaag tccatatcat ctttatattg catatgaatt tcagcggtat
     6961 ttttatatag cgcttcagaa gtctttatac catttataac attagtagaa aaaaatagta
     7021 ttgtaatgca gacaaaaagt gataaaacag cgtttatttt ttttaacata ttttgaattt
     7081 cctttcaaat gtaaataaaa aagagagttt aaactctctt ttttaataaa cattccacca
     7141 accagtattt ccagtaagtg tggcattgca tgttgcataa gctcttactc cagctttctg
     7201 aactgatcgt acttgaccgc ctcgtccatt tttagctgtt gcactatgtt tagctctagc
     7261 atggtagtaa tttgcataaa ctttaaatcc attaacccca cagttgaaat ccccgccgcc
     7321 agcagcatat ctcaatttac tagaagaaaa tccttcttct gtagcttcca ttcctcctcc
     7381 gtaaatggcg gtatggtctt cgggtacatc tagatttaaa gcttctgtgg cactagctat
     7441 tcctccacta gttaataaga cacccattaa agcagaaaga cataaaaatt ttttcataac
     7501 ttatttctcc tatctttttt actagaatta ttttatttta gtgtgtttta taaaataaag
     7561 aacttttttg gcgcgttagt atttttatga taaaatttta tgtaaataat cgtttacata
     7621 ggagaaaaaa tggaggattt aggatctaag ctaagagaat taagagtgaa aaaagggtat
     7681 actcaagaat atgtggcaag aaaatttaac gttacacgtc aaactatttc aaagtgggaa
     7741 aataacaaaa caacaccaga tatagagaca ctaaaaaaaa tttgtgaatt ttatagtact
     7801 aacataaatt ctattttaaa tgaagataat tcaaaaaata aaaaaaataa tttactggta
     7861 ttgacagcta ttatttttaa tatatttgtt actatattag gtttatttga attgattcaa
     7921 atgaattttt ttagctttac acatggaata tttacttggt ttgctctatc tggattaata
     7981 gtaatagata tcttaaatta tttttatatt tactattctt atttaaaaaa tgaaaaattt
     8041 ttttatacaa tgtttttccc cctagttatt ttgattattt tattaagcta atatgaagta
     8101 gttagttatt taattgcata gttgacataa tgtacactat gccaagtagc ccctctacca
     8161 atggatagag acgctcactt tgtgattttt aattggtcta gctaaaagca actttctaaa
     8221 gcttgatttt tcgcggttat ctatatctaa ttctttgtgc atttttatct gtcatttttt
     8281 taagacttgt tattttttgt aaaatatggt aaatttttgg tagcgcttca taagcgcgtg
     8341 ctttttcaag aataaacact cattattttt taactggctg ctcggaagag cagtcttttt
     8401 tattatttat ccgtctcttt tacaaacaat tcttcgttag gactgttgta ataatttgga
     8461 ttcaatttca attcaccatg tttattccct ttattgatta ctgacaataa catcggactg
     8521 aagcctagaa ttcctagtaa aatagaaccg aatagaagat tttttcttgt catgaatgtt
     8581 ccctccagtt atttccaaag ttgccaccat ttttgcttct tcttttttgt gtgtttttct
     8641 atcgattctg tttcttcaga aagaatagta gttgattcat cttctggagt ttcttccgta
     8701 taagctaatt gtaactgttc ttgcagtttt tcgatctgtt gatttgcctg tagggtgagc
     8761 tgttgttgct ggtcgagtaa tttttgcata ttagaattat tttgctcttt gagggaaagt
     8821 tgttctcgta aaaattcatt ttcttcacgc aacttatcta gcaactcgtt tacaatcacg
     8881 tttttgcttg atatataaac gtttttatcg ttttcttgtt catactcatg caacctatat
     8941 agtaaccttt ggttacctaa gttgctagca agttgcaata ctccagaacg taatacaacc
     9001 ttataaccac gctcattttt agctaataag gacgggtcta atttatcaac atgtttagag
     9061 attgcttgcc tacttatttc aaataattca gataattcct ttattgtaaa ggtttctttg
     9121 ggttgcatga taattgcctc ctaaattgtt aggtattatt tctctaacca attatctaag
     9181 gtaattgaag gtatattttc ttcaaaataa atttgcagag atcctatctt atttcctttg
     9241 taggctttaa tttttttgat ttcaaaagtt ttaaaaatag cagggtgagt tgaattaacg
     9301 tttaaaaatt ctttttt
//
