LOCUS       NZ_OP378669             4165 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Serratia marcescens strain SER00066 plasmid pSER00066_3, complete
            sequence.
ACCESSION   NZ_OP378669
VERSION     NZ_OP378669.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Empirical genomic methods for tracking plasmid spread among
            healthcare-associated bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2022) Medicine/Infectious Diseases, University of
            Pittsburgh, 3550 Terrace Street, Scaife Hall S853, Pittsburgh, PA
            15213, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Sequencing Technology        :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028738375.1-RS_2024_03_26
            Annotation Date              :: 03/26/2024 01:30:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP378669.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="SER00066"
                     /isolation_source="Clinical"
                     /db_xref="taxon:615"
                     /plasmid="pSER00066_3"
     gene            complement(295..705)
                     /locus_tag="PWB75_RS00005"
     CDS             complement(295..705)
                     /locus_tag="PWB75_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714791.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKSIKVID
                     TSELIRVFGSLDGVIDDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(705..896)
                     /locus_tag="PWB75_RS00010"
     CDS             complement(705..896)
                     /locus_tag="PWB75_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_025714792.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELLDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1368..1559)
                     /locus_tag="PWB75_RS00015"
     CDS             complement(1368..1559)
                     /locus_tag="PWB75_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_022631206.1"
                     /translation="MNKQQKTAVNMAKFIQAQSLLLLDRLNDLDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1937..2587)
                     /locus_tag="PWB75_RS00020"
     CDS             complement(1937..2587)
                     /locus_tag="PWB75_RS00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064176659.1"
                     /translation="MRYVKISLSVATFLLFWYVSSFFTGCSDGWGSSSIGLRGACSYHG
                     GVSKLPIIIIFLGLIASFFVFFLFPDSESATSKKDTFPVVNKESNESIPKIKTNTHNEI
                     KVKRELKIEIENLNDKKFSKIFIYSDGLFIKEFNPENKMKVSTKRINKNKILKISNLLR
                     EGKGGAINPILDVEAKGYLVRLFENHAHESKTYFIDRSEKQAFSDIKELINSV"
                     /name="hypothetical protein"
     gene            complement(2604..3539)
                     /locus_tag="PWB75_RS00025"
     CDS             complement(2604..3539)
                     /locus_tag="PWB75_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_169318907.1"
                     /translation="MTKPPLDLFNDRLPNKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRTGAAIDWSDRNAPAPTLTITNPENGHAHLLYALETPVRTAPDGKMK
                     PLRYAAAVENALRKKLEADEGYSGLICKNPNHGHWKIAVWQPELYTLDWLADSLDLSAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERASAYNLQFSSPLD
                     ENEVNGIAKSIAKWTHSNFDEASFKKYVEDTHRSYIQSLRGKKSRGGGRKKMPEDKILA
                     IKEMVSEGMSYREISKLENVSIGFISKHCK"
                     /name="replication initiation protein"
     gene            3922..4113
                     /locus_tag="PWB75_RS00030"
     CDS             3922..4113
                     /locus_tag="PWB75_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_274066257.1"
                     /translation="MFFLPENGERGRNLLRGREALPAQPENRFSARSGIGFVDGRQSRY
                     GKTPDDSPHDSPAIRLVT"
                     /name="hypothetical protein"
ORIGIN
        1 gtcaagtcgt cataagttac tgatagtgtg gagtgagtcg tcgatgactc tatgacgagt
       61 cgtcggtaag tcgtacacga atcaccgacg acttggattg accttacaga aggtaatact
      121 gcgtaattta ataaaaccta accaactgat aataaggagt tatctgcaaa attttaattt
      181 tgcgtggtgt gtgggtattt ttcgtggtga ggagtgttcc cggatctggc gatttttggg
      241 aaggttcagg aacgccccgg gatggccgga gaggaaaact caagcatggc gggatcagga
      301 ttttttccag aatttccacc acggggaatg gggtggtgaa gaaggttcgt gcttgtgctc
      361 cagaagctgc atagcctgac gaagggaatc aatatgctca tctcgagcct taacagcccc
      421 tttaagacca tcgttttcag cttcaagtaa agcgattcta tgttgtaaat catgtaaacc
      481 aacgctgtta atacctgtag attctgcgtc actttttaca tcatcgatga caccatcaag
      541 agaaccaaac accctaataa gctctgatgt atcaataact ttaatactct tcccgtgctt
      601 tacaacagag gtgtaactta acttaccact gttaatcatc ttgtaaatag tagggcgact
      661 aaccttaaca agccgtgctg cttcactaat agacactctg gccatcagtt accatccagt
      721 aattcatcca gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc
      781 tcacaaaggt cggcctcagc atcgagatca agctcgttaa gcttctccag caggagcaga
      841 gactgattct ggatgaattt agccatgttc agcgctgttt tctgttgttt cgtcataagg
      901 gacctcagag catcagtgct cagtgtaaaa ccggtaaggg ggtcggatgt acgaatggtt
      961 cagagaacgg atctgaggta atcctgtagt tattttgtag tttatctgta gtcatcgcac
     1021 taaaaatcag gattaagtca tcaacttttg tagtcttcct gtagtttacc tgtagtccgg
     1081 aaaaataatc cgttcatgca ggcatgaacg atacagtgag gaacgctttt tttgtcacga
     1141 tctaccctta acctgcgtaa atcacccgcc atgattgatt atttttcaac ctcaaaatgg
     1201 tcatacacct aacgcagctg cgattttcat cgcaacttca tgaaaaataa ataaaaaaga
     1261 aaaaatacag ggattctggg tattaccggg gtaataatgt taagcgctaa cacaatgaaa
     1321 attaaggcga tgccggtatt actccggtaa tacttagaac ggagacatta cgcaccgtcc
     1381 tgctgctcat tcaggcgcgc tgacaggctt cggtaaaggc gttccgcatc ctcatggagt
     1441 ttctcgcaga aatcagcctc atgatccaga tccaggtcat taagcctgtc cagaagaagc
     1501 agggactgag cctggatgaa tttagccatg ttaacggctg tcttttgttg tttgttcatg
     1561 aaggccccgg cgcgacagtg cttactgtac gttctgatgg ttttccgctt cctcgctcat
     1621 tcgctcactc cgttcggtca tgagactgcg gcgagcgtta ccggctaatc acggtgagaa
     1681 tattcgcagt gaatgtggga ataattcgca aaagattcgc accattctca cgggggtttc
     1741 gggggatgca atcccctgaa ccagtcacgg tggaccctcg ccagagggga ctccgtgtgt
     1801 actggcttaa agggtagtgc agtatgtcag gttctgtgct ggctgcaaaa aaaagccctc
     1861 tgctagagag ggctgttatc tgatataggt tgtttatgaa caccttcgat aaaagggttc
     1921 ataaacaacc ttcccattag acgctattga tgagttcttt aatgtccgaa aaggcttgtt
     1981 tctccgatct atcaataaaa taagttttac tttcatgcgc atgattttcg aacaaacgca
     2041 caagataccc ttttgcttcc acatcgagaa tgggatttat agcccccccc ttcccttcac
     2101 gaagaagatt acttattttt aatattttat ttttattaat ccttttggta ctcaccttca
     2161 ttttattttc aggattgaac tctttaataa aaagaccatc agaataaata aatattttag
     2221 agaatttctt atcgtttaaa ttctctattt ctattttcaa ttcgcgctta acttttattt
     2281 cgttatgcgt attggttttt atttttggaa tagactcatt actttccttg tttacaacag
     2341 ggaaagtatc ctttttgcta gtagcacttt cactatcagg gaaaagaaaa aaaacaaaaa
     2401 aactagcgat gaggcccaaa aagataatga ttataggtag cttactaacg ccgccatgat
     2461 atgaacacgc tcctctcaac cctatggatg aagaacccca gccatcagaa caccctgtga
     2521 aaaagctaga gacataccaa aacaataaaa aagtagctac tgaaagggaa atctttacgt
     2581 aacgcataaa tcaccaattt atttcatttg caatgtttgc taataaaccc tatggataca
     2641 ttttctaatt tggagatctc tctataagac atgccttcag aaaccatctc ttttattgca
     2701 agaatcttat cttctggcat tttcttccgc ccccctccac gactcttttt tcctcttaat
     2761 gattgaatgt aacttctgtg tgtatcttcc acatacttct taaatgaagc ttcatcaaaa
     2821 ttactatgag tccatttagc aatacttttc gcaataccgt tcacctcatt ttcatcaaga
     2881 gggctcgaaa attgcagatt gtaggcactg gcacgttcat agcaggcttg cagccactgc
     2941 tcatactccg gccagccctg gcggatcgcc cggtatgccc acttgcgggt tttatcgaac
     3001 agagtgcagt tacggcctaa accatagtcg gcgacgattt ctttgtcgtt cgcggcactc
     3061 aggtcgagcg agtcggccag ccagtccagc gtgtagagtt ccggctgcca cacggcgatt
     3121 ttccagtggc cgtggttcgg atttttgcag attagtcccg aatacccctc gtcagcctct
     3181 aattttttac gcagcgcgtt ctccacggcg gcggcgtagc gcagcggctt catttttccg
     3241 tcaggagcgg tgcgtaccgg ggtttccagc gcgtacagca ggtgcgcatg tccgttttcc
     3301 gggttggtga tggtcagcgt gggcgcaggc gcgttccggt cgctccagtc gatggccgca
     3361 ccggtgcgat cgacgtcgaa tcccagccag aacatggcgt gcggctggtt gaactggatg
     3421 tatttcgcca ggatcgcgcg ctctttcccg gcgatgcgca cgccgaagtg caaatcgtcc
     3481 gagaagtacg gtttgtttgg gagacggtcg ttaaaaagat cgaggggggg cttagtcact
     3541 cacgcctgaa cttatggcgg gctgttgtct tgctgggtgc atgtgctagt atccttctta
     3601 ggaatttagt cctgcgccct cgttatcgtg atcttccgcc aagttgattg cacgatttcg
     3661 ggggcgtttg ctttctgggg ttaaaactac acccagttca ggcacttgtc gacctccccc
     3721 cgatttacca taacatctgt tatcagcacc gcctcaggcg ccctcagcgc gtttctgacg
     3781 ggaaaccggc aaaacaggca gcaaaccacc cgaaaacccg ccagaaacgc gcaggctctg
     3841 tttttacggc atgcatgagt atgcgcgcaa gtgcatagcg cggaaattca cgccattcag
     3901 ccggggtaag tcctgtttcc gatgttcttc ttacccgaaa acggtgaacg gggacgcaat
     3961 ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa accggttttc agcacgttca
     4021 ggaatcgggt tcgtcgacgg ccggcaatca cgttatggca agacgccgga cgactcacca
     4081 cacgattcac cggcgattcg actcgtgact tgactctaga gtcaccacgg tcaaaaaaac
     4141 gccaaagcaa cgattcgact ctaga
//
