LOCUS       NZ_OP378660             5251 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Klebsiella pneumoniae strain KLP00203 plasmid pKLP00203_4, complete
            sequence.
ACCESSION   NZ_OP378660
VERSION     NZ_OP378660.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Empirical genomic methods for tracking plasmid spread among
            healthcare-associated bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2022) Medicine/Infectious Diseases, University of
            Pittsburgh, 3550 Terrace Street, Scaife Hall S853, Pittsburgh, PA
            15213, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Sequencing Technology             :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028738285.1-RS_2024_03_26
            Annotation Date                   :: 03/26/2024 01:35:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP378660.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KLP00203"
                     /isolation_source="Clinical"
                     /db_xref="taxon:573"
                     /plasmid="pKLP00203_4"
     gene            complement(join(4647..5251,1..751))
                     /locus_tag="PWB78_RS00005"
     CDS             complement(join(4647..5251,1..751))
                     /locus_tag="PWB78_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            complement(1384..1596)
                     /locus_tag="PWB78_RS00010"
     CDS             complement(1384..1596)
                     /locus_tag="PWB78_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1600..2085)
                     /locus_tag="PWB78_RS00015"
     CDS             complement(1600..2085)
                     /locus_tag="PWB78_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_142485265.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEHSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2123..2800)
                     /locus_tag="PWB78_RS00035"
                     /pseudo=""
     CDS             complement(<2123..2800)
                     /locus_tag="PWB78_RS00035"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2763..3083)
                     /locus_tag="PWB78_RS00025"
     CDS             complement(2763..3083)
                     /locus_tag="PWB78_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3720..3881
                     /locus_tag="PWB78_RS00040"
     CDS             3720..3881
                     /locus_tag="PWB78_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(4034..4654)
                     /locus_tag="PWB78_RS00030"
     CDS             complement(4034..4654)
                     /locus_tag="PWB78_RS00030"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     Typing          complement(3198..3370)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          3317..3830
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.424"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1269..2773)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.016"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttattgg tatttccgcc tcaacctccc gtacagatat accggaaaca ccagtgtcaa
       61 ccatgcataa aaccttggcc gtttcattta cgacgtcctc cctctcccca aaagaagata
      121 ttcttatctt ttcatttaaa cctatatgtc taattccctt tctaaaaaag ttataagctt
      181 ctttaattat gtctggtgat gcggcattat tacctgcttt cgctaagtat gaattattag
      241 ggaagaatgg tattttttta atacctcctt tataatgccc accaaacttg attgtttccc
      301 aggtgtcgct ctcatctcta gtaaataaat ttgctttaac cctagatgga taatagtcta
      361 atgattcaaa taaaatccga tttttaataa aagcaacttc ataactgaac cgattcccct
      421 caacagaata aaattcagcc tcaaacttta caggagcctc attcgtagcc gaagaaagag
      481 catatggctc atagcaggga atccgtttac cttctttcaa atcaccgctt tctgttgcga
      541 taaattttat agcttcaaaa gccatgagaa cgttagattt accagacgca ttagcaccat
      601 aaatcccagc actcatgagc gttccaacat tcattccatc atttttatag atatgagttg
      661 ttaaatggtt actagaaccg gatgcgctca agtttaatgt cgatttttcc ttaaccgacc
      721 taaaattttc aatagtcaaa taaacaaaca tgcatacctc ctaacgggga tttttcgcca
      781 aaaaagcgaa aaatcaatca taggaaagat atcctatatg ttttaatttt ggaacaacat
      841 cttccttaaa aaacgttgtg ctatttataa ttacacgcta tttaccataa catctgttgc
      901 ccgcaccgct ccggacaggt tcgccacaaa cggggcggca gtcaggtaaa accggcaaca
      961 aaccacccga tacaccgccc cgttcgtgac agataaattt tacccggctg catggtcatg
     1021 cagccagatg catacggtct gatttcaggc cagaatccgg atgaacgcct taaaaacagg
     1081 aaaatgcttc tccagccagt cccagatctc cagcgccatg tacaggcaat tcagcaccgt
     1141 ctcaatcacg ttaaacagtc tcataatccc tccgttgtat acgctgtgat ggaaacatca
     1201 cgaccgtatt tagcgggaaa tttatgagcc cttcttcttg gggtttgtca ctggatgcgc
     1261 gcagtcagcc cagcgtcgga ccgtcatagc tccgctgccg ggaacgggtt tccgccagct
     1321 ctttttcatg ctgtttctgc tcgtatgccc gttcggcggc caccgccatt tcatgccgct
     1381 gcactactgg ctcaaacgct gtaactgcct gctcaagttc gccacctgct gactcaagcg
     1441 cgtgacgcgc tcgttcagcg ccgcgttctc ccgttgcata agaccagaca tcttctgcca
     1501 ttccgcgaag gcgctctccc actcgtccag cctttgcgag tagtcctgat gtagctgctc
     1561 taatgcggtc agcaactgtt tttccagttc cgtcatgggc tatttcctct ccagtaccat
     1621 ccagttccct tcctccccgt aagctcccgc tttcgggttc actttgacgc aggacagttt
     1681 cttctccccg cagagcgaca gctgcacgcc gtggttcttc tccgacagca tggcctgcgt
     1741 ccgctcctgc gcccggatag tctggtaatt cccggtgatc tgctgcccct gccaccacag
     1801 aatgctcccg ctcgtggcga tcagcagtac cgagaccagg gtgatggtca gccacgtccg
     1861 gccgaccata cgcagcacgc tcagccggtt cgactgcatc gccgcggtca taccctgctc
     1921 gtgggcgctg atggcgtcgc tgattctctt cgcgcttgcg ctcagttccg cttttacaga
     1981 atgctcgtgc tcgctgaatg cggctttcag catctcgccg gtactctgct gctgcgcttt
     2041 cgattgctgc tccaagtcct tcgccagcgc taaaagactg ctcataaatg gctcccttca
     2101 gtcggatgtt tcgccccccg tccgggtcgg caatgctgat actgtttttt gtggtgcgga
     2161 ccacctcaaa acctgccgct gtgagcgccc ctgtgacgtc ctcgcgcgtt ttgagctccc
     2221 cggatgaggc taaggccagc aacccacgtg taatcgcctg agcggcttcc agcttcgttt
     2281 ccggcagtgc ggacggcgtg accagtaacc gtcggttctc cggggcgttt ggatcatgca
     2341 gccccagcct gccgttcacc acggtctgcc aggcatcgat gcgcggccgg tcggcgcgat
     2401 cgtagtacgg ctgcaggcgt ttcccggtca gcagttcagt gttggggatc aggaaattca
     2461 gctccagccg ccccttgtcg gtgtgctcca cccacagaat gctgtactgg tctttatcga
     2521 gcccgggcat cagaacccgc tcaaagctgg ccatgatctg ctcccgctgc ccgggcggca
     2581 gttcagcctc cgcgaacgac agcacgccgg aggtgtactt tttcgcatac ggcgaggcat
     2641 cgataagctc ccggacttcc tccggttttc cctgcagcac ggtcgccccc tcgcgctgcc
     2701 ggtcttttcc cagcaggtaa tccaccggac cggcaccacc accgcgcccc cgggggtgaa
     2761 atttaacgat catcatcccc ccctttttcc agcaccgcat gccgcagccg ctccagtccc
     2821 gcgtcgattg ccatcagtgc ggccacaatc tgcacccggt catgaccggc accaccggca
     2881 ttcactttcc gggcgatctg gttcaggttg ttccccatac cggcaagctg gcgcagcagc
     2941 gccggcgata tcgacggcag tctgccggca cgcgccggct tctcatcgag gcaggtctga
     3001 cgcatccacg ccgccagctg cttgccgtcg caccgctcga gcagccgccg gtgttcgtct
     3061 tccgtcaccc acatcgtgag catcttgttg cgtttgtctg ccagagtagc ctcctgataa
     3121 caccgggacg ggcgggaaat gtttcccggt cggcacggtg ttggacaagc cgcaggcgcg
     3181 tcagtcgctt ttagcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga
     3241 gtgtatactg gcttattatg ttggcatgag tgcggtaatc aggaaagtgc ttcatgtgac
     3301 agaggaaaat gcagctgcgg gcgtaaggca gaaaatggaa gttcagagga tatgacgcct
     3361 cctcgctcac tcagtcgctg ccgctcctgc cgttcggctg cggcgggcgt tacctgcaca
     3421 cgaagaagag cgaaggcata tggcatgaca aacggggcag aaaaaggatg cgccgctgcc
     3481 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     3541 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggtg gctccctcgt
     3601 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     3661 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     3721 tgcacgaacc ccccgttcag tccgaccgct gcgccttctc cggtaactat catcttgagt
     3781 ccaacccgga aagacacggt tcatcgccac tggcagcagc cattggtaac ggattagagg
     3841 aagtgagtct tgaagttctg cgccagttaa ggctaaactg aaaggacaat ttttggtgac
     3901 tgcgctccct ctaagccagt tacccggtac ataagttggt agctcatgta ccttccaaaa
     3961 accaccgttg gtagcggtgg tttttatttt tcatagatta cagcctgtaa taagccatgg
     4021 tcaatggatt acttcaaaag tcgtcaatcg ccttaagtag ctcatggaat gatgtataac
     4081 atggaaatag ataaggttcg tgcgtgtttt tataattagt tttagcgtaa atatctagct
     4141 ttcttgcgtt atctttcgca gtaccaatcc tctctcttat agcaaggtaa gttagtttac
     4201 tacctttatc atagttagtt attaattttt ttagcgggga gctagacatc aaattatcat
     4261 acgacgaaaa atctttggtt gtgaagccga aatgcaacaa taaccataat tcaaaacaaa
     4321 cacaggatat tgctatattt atcccatgcc tattagcttt attccatgcc ttttgatgac
     4381 atgaaataga cgaaggcgtc agatgctctt gatcataaac aacccaaaag acgtcacctt
     4441 ctgcggaaga tgatgaattc tttttcttta tcgcctcatc caccaactgc tcaggtgtat
     4501 tttttcttgt tttaggtatt ttaaatacac ttaaagttct tttagatctg tctgaaatat
     4561 aagcctcaag gtataatggt tcggttttct caccctcaca gtatatatac attacttgtg
     4621 ggactttctt attatttcca tgttttttac tcatcagaaa gatccccgtc aaactcttca
     4681 aaaaaaccat cagacaattc tttgacgtca atatccggca ttaccaagga tatatcacca
     4741 gatataaatg aggctacctt taagtagttt atgtcgggaa ccccaccaaa cctaccctca
     4801 tcataccaag ctgcgtaagg tgttgttgtt tttatctttt tcttatcaaa atcatctaag
     4861 ctataaagag acgacgcacc tttatttttt tcacaaaacc atatctgatc tcttctcatt
     4921 ttctctggag ccattaactg catattatgg gttgagaata caagttgtga gcctttttta
     4981 ttgacatcag gatcattaaa taacttaaca atcaagtcag caatatgcgg gtgcaagcta
     5041 ttatctaact catcaacaat tacaaccata ctatcattaa acgcagatct aattagaggt
     5101 atgatttcaa ataatttttg ggtgccttca gactctctcc ccatagggaa agaaacactc
     5161 cccccttcat cagtaggatg agagaacata taacgatatt tataatcttc ttcaattatc
     5221 tcctttattt cttgagggat ttcctcatcc a
//
