LOCUS       NZ_OP378651             9506 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Klebsiella pneumoniae strain KLP00155 plasmid pKLP00155_6, complete
            sequence.
ACCESSION   NZ_OP378651
VERSION     NZ_OP378651.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Empirical genomic methods for tracking plasmid spread among
            healthcare-associated bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2022) Medicine/Infectious Diseases, University of
            Pittsburgh, 3550 Terrace Street, Scaife Hall S853, Pittsburgh, PA
            15213, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Sequencing Technology        :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028738195.1-RS_2024_03_26
            Annotation Date              :: 03/26/2024 01:32:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP378651.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KLP00155"
                     /isolation_source="Clinical"
                     /db_xref="taxon:573"
                     /plasmid="pKLP00155_6"
     gene            complement(join(8981..9506,1..206))
                     /locus_tag="PWB88_RS00005"
     CDS             complement(join(8981..9506,1..206))
                     /locus_tag="PWB88_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(206..2008)
                     /locus_tag="PWB88_RS00010"
     CDS             complement(206..2008)
                     /locus_tag="PWB88_RS00010"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_240266022.1"
                     /translation="MRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARR
                     HVWNYAYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWC
                     AGGLAVGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEP
                     VPVNALADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATN
                     ALIRCVRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYAL
                     KNLLIAGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDV
                     KGLITRLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQI
                     IIARDEFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDL
                     PAEFVKTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGD
                     SFIQKEARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTD
                     PLAEFRPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3863..4012)
                     /locus_tag="PWB88_RS00015"
     CDS             complement(3863..4012)
                     /locus_tag="PWB88_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4097..4354)
                     /locus_tag="PWB88_RS00020"
     CDS             complement(4097..4354)
                     /locus_tag="PWB88_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(4364..6049)
                     /locus_tag="PWB88_RS00025"
     CDS             complement(4364..6049)
                     /locus_tag="PWB88_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6452..6871
                     /locus_tag="PWB88_RS00030"
     CDS             6452..6871
                     /locus_tag="PWB88_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7177..7440
                     /locus_tag="PWB88_RS00035"
     CDS             7177..7440
                     /locus_tag="PWB88_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            7430..7729
                     /locus_tag="PWB88_RS00040"
     CDS             7430..7729
                     /locus_tag="PWB88_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7791..8168)
                     /locus_tag="PWB88_RS00045"
     CDS             complement(7791..8168)
                     /locus_tag="PWB88_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            8237..8416
                     /locus_tag="PWB88_RS00050"
     CDS             8237..8416
                     /locus_tag="PWB88_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8444..8974)
                     /locus_tag="PWB88_RS00055"
     CDS             complement(8444..8974)
                     /locus_tag="PWB88_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..9506
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3182..3760
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..206)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.59e-24"
     Typing          complement(8985..9505)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="5.43e-113"
ORIGIN
        1 tatttggtcc ggaccagcag ccctttcgcc accaggctgt cgaggaagcg gtagaccggc
       61 tgcaccgccg caatccccag ccgccgcgca accagctccg ccgtggaaca cagctcttcc
      121 cgcaaccagc gcagggtcag ccgggttttc tccgcgttcc gcgcctgtcg tttcgcgtgg
      181 ctgacgttgt acagctccag cgccatcagt aaatccccgc aaaatcatca tccgctttcg
      241 gcccgccgcc gtcggccggg ggccggaact ccgccagggg atcggttttc gccagcaccg
      301 ggaaacgctg caacgggatt tcccggcgct caacccgcag cggactgctg aacgccatcc
      361 gggccacacc cagcccggtc atgaccgcga acccgtccgg caggtgttgc agggtgttca
      421 cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc cccgctgata tgctccacgt
      481 tccccgcctc ccggctggca cggcggcgct ccacgtccac cctgatttcc ccggtctgcc
      541 ccgccgccca ctgcgcggac tccaggctgg ccgcccggta aaaccagcgg atcggcgtgt
      601 tatccagtac cgtggttttc acaaattcag cgggcagatc ctgcccgcac tgcccgaaat
      661 cgcccagcga ctggtgcgcg agcaacagat tgcagtcccg gctgcgcagc gtccccagtg
      721 cgttcagtac atacttcgac agcagatatt taatttcatc gagcatcacg ctgacgtgtg
      781 gccactcccg gaactcgtcc cgcgcaatga tgatttgcgc gcagcgggca aacagcattt
      841 tttgcacacg gatcaccgcc tcgtcgtcca tcgagccgat aacgtacagg cagccgccgc
      901 cgttgataat ccccgccaca tcgatgccac tgtccgtctg caggacgctc aggtcagcga
      961 cgttttccag cctagtgatc aaccccttca cgtccttttt cagggcctcc ggcagcgcgt
     1021 acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg aaactgctcc gcaatcagct
     1081 tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc tcgcccaggt
     1141 taagcccggc aatcagcagg tttttcagcg cgtactggtc gagatcgcgg aacagattca
     1201 gctgcgccgg tttccccggc cgcagatcca gcagggtgaa gtccggacag tgcgcccgga
     1261 agacgtgcgg cgcccatgcg tcccctttgg ggtcgaagta caccaccgcg tccccgaaat
     1321 cgcgtacgca gcgaatgagc gcgttggtgg ccatcacgct cttgccgctc ccgggcaggc
     1381 cgaggatctg aatgttggtc ttgcgccagg tatcacgcgg aaccagcacc ggcttgccct
     1441 gctcatcctt acccagaaac accgcctgct cctgccgggc ctgctccgag gaaaaatcgg
     1501 ccagcgcatt aaccggtacc ggctccccga aatgcaggtg gcgcacgtcg gtcatctctc
     1561 ctgtccgctg gctgctgcgc cggctctcct gattcagttt cgcgttcacc gcctcgtcag
     1621 cagccgcaaa atgaccgtca agcagcagga acgccagcag ccgcgccaac acaatcccga
     1681 cagccagccc gcccgcgcac caggcaatcc cgaacaggtt agccggctgc agcaggtaat
     1741 aatctgcgtt tgcgtagagg tccggacgca ggacggtttc tccccagctc tcgataaaca
     1801 cggcgacaac cagccccccc agaagggcca gaccggccgc gccggtcagc cagtacgcgt
     1861 agttccagac atgtcgacgt gccctgacca tccgcccggg cgaatccagc gaggccagca
     1921 ccgaaaatgg cgctggccgt ttattatcgc gcttatagcg atcggcatac tcccgtcgct
     1981 ccttatgact cagccccaca aaacgcatcc gtccggccca aagttcagcc agaccgaaaa
     2041 acaggccgat aactaggaaa gccccggcca gaccgtaata ggggtttgac gctcaacgga
     2101 acggtttcac acgttaagga ctttttgtcc gataaatcag aatattgtgg acagtgaaaa
     2161 atgtgcctaa agcggtgtgt ttttaatcgg caaatggcac ttaatggaca ccattgtcca
     2221 ttaatcgatt tttcagacga cttctctcgc ctaacgtgag ttttcgttcg actgagcatc
     2281 agccccctaa tactcctggt acagccagct gacccacgga taaatctgcc gggccagctc
     2341 cggaatgtgc cacttccagg caagcgaatc cgtattaaac atgttctctc tccttcagac
     2401 gatggtttgc cggcgtgatg tgcagccccc gctgcacaag ccgcggacat gccccaaggc
     2461 atggcggcgg cggtcacgtc cgtggtgatt gcgttcagct ttcaccacgg aggccagcct
     2521 ctttccgccc catgcggaaa aggcgcagcc ccggaggaac aacgagcacg ccccagcgtg
     2581 tgagaggaaa aggcggcacc ccagtgcggt cttttccggt gttccggccc ccgtcagagt
     2641 tcgccatagc gagcggctga ccacccgcaa aatcaggagc agctttgctg cttcgccagg
     2701 tgcgtccagc accgtggatt tttgtgggtg gtcaggcgtg agcctgacgg gggccgggtg
     2761 aatttctggc gtggccggcg tccagccgga cggagccaga aagaggggga gagaccgtgc
     2821 gtcggtggcg tccagccagc gtaagcaggg gatatccccc tgaggtcagc cacgccctcg
     2881 cggggatgac ctgtatttct ccgtaaggag aaaaaagcaa cagagatagg ttaacgtaaa
     2941 atgttcagcg aaaaaccgat atgattcacc tatcctggta atttattatc agggaatata
     3001 aagacttaat caggggacaa tatgccacat cagcgcgtcc ggtgtccgtc ttgtaggtgc
     3061 cggggaaatt gcgcctcctc gctcactcgg tcgctacgct ccggccgtga gactgcggcg
     3121 ggcgctgcgg acacatgcaa agttacccac agattccgtg aataagcagg ggactaacat
     3181 gtgaggcaaa acggcaaggc cacgcaggtg gcgtttttcc ataggctccg ccctcctgac
     3241 tgagttcaca taaacagacg ctttttcggt gcatctgtgg gagccggtgg ctcaaccatg
     3301 aatcagtcag gagtggcgaa acccgacagg acttaaagat catcaccgtt tccgatgaat
     3361 cgctccctct tgcgctctcc tgttccaacc ctgccgtttc ccggatacct gttccgcctt
     3421 tctcccttgc gggaagtgtg gcgctttctc atagctcaca ctctggtatc tcggctcggt
     3481 gtaggtcgtt cgctccaagc tgggctgtaa gcaagaaccc cccgttcagc ccgactgctg
     3541 cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacgcaa aacgccactg
     3601 gcagcagcca ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc
     3661 tcttgaagtg tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct
     3721 gcgaaagcca gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca
     3781 cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct
     3841 caagaagatc ctttgatctt ttctactgaa ccgcgatccc cgtcagttca gaggaggacg
     3901 atggtgccac cgttccaccc tgaacatccc ggatatagtt tgcctgacat gcgaccagaa
     3961 gaaagcctga aaaaatcaat ataaaaagaa aaacaacctt tacctttttc atataccttc
     4021 cctgtattta aaaaccccgg tattcatccc tgaacaccgg aagtctgtat ttcaaatcat
     4081 ccttgataac ttttgcttac cagttaccat cccggtaaac aaaggataca aagtaatcaa
     4141 atttactaat atcgattaca tttttaaagt gtggctgtaa tatcgaaacc catgattttt
     4201 caacatcaaa ccaaccatta ttaatattat cctttaaagg catccccaga ctttcaatga
     4261 cagaaccatc atcaccgaag tcttttgagt attcaccacc tttaaactct tcggttttct
     4321 tatcaaacca atgaatatgt aatttaagcc ccatatttac ctcttaaaga tattttttaa
     4381 tgtttcgttt tggatccggc cctttaacct gcttacccgt ttttggatcg aatgccccga
     4441 ggtgttcgcc atcactggcg cggtaccctt caagctcacc gtgctgggaa tcccattcat
     4501 aaattttacg ctttttatca ccataccatc gggcccgctt accaccacca ccttgtttag
     4561 gggttttagg tttaccctct ttcaactctc ccaacccctt aatatcttca gtcttgggat
     4621 caggaaaata atcatggccg taatctttag ttcccttacg aggttttttc ttttcctcat
     4681 ttaatttatt ttctgcatcc ttcgcttttt tctccttttg tttccggctc tccatcgccc
     4741 cggaaagcgc agccttatcc ctggataagg attcattcat ttcgtttaaa gatgtctggg
     4801 cagcttttaa tttctcattc tgattctgaa tatccttatc aagatagcga acctgatcct
     4861 gccacccgga gccatattca tgaggcgggt tataagtcac aaaatcatct ttatttgcct
     4921 ctgcctcttt taccttttta tcggcttcct gaacagcagc ccttgcaccg gggatcgcct
     4981 tctctgtcga tgcaatgcga ctgtttaagg tcgcaatatt tttatcttca gcatccagct
     5041 cagctttcgc tttatcatac tctcttttca gcccctcttc cgggtgagca gcgtcccatg
     5101 cctgctggcg acgcttttct tcctccagac gctgttttac ctgtgccgga gtaagaacat
     5161 ccgtcaccga cacatagacc ggtttctgtc cgctctcttt cgggaaacga ataacggctt
     5221 cacgggagtt accaccggcc gtaaaaccag ccggacgtga atcacctttt tcagcaataa
     5281 tgccttttgg cggggctttc gcggccggaa cacctttagg tacgctcacc tgcagagacg
     5341 ggagacccgg aatcgacaca ctgaataccc ccggacgttt tgtcggtttc gcatccacca
     5401 caggaacact cattggccgg ccggtgacaa ccgcaatatg ctgccgctcg tccttcacaa
     5461 tatccgccac acgtttagtg acgctgaccg tcgcctggtc cagcggcagg gagctgaccg
     5521 gcgtctccgt taccgtgtct gccggcaatg acgtcacgat ttttgacatc attttcggat
     5581 catcttttgc tatctcagaa ggcagcactc cgtaaatcgc aatcccccac agaccaaact
     5641 taaacggacc tttcagggca gccagcacat cagcaacagc ggccgacaac gcatcgccgg
     5701 aaacggataa agccagtcct tcagcccccg gagtggccaa ggcaggcaga ccgaaggcca
     5761 tcgcggtggc cgaagactgt ccgccaccag agctcccgct atgattgccg ctgttaccgc
     5821 cattaccacc gggtttactc ccgccgttac caaactcccc cgggttatag ttatgaatat
     5881 ccccgttagg tgtatgcgtc gtaccccagc ccggcccgct gtttggatcc gtaccaccac
     5941 ctgatgaagg gccaccttta cctgatgtac cgttcacatt cccactggcc tgaccaccat
     6001 tgtgtcccag acctgaatta cccggacctc gtccgtctcc gccactcata tacttttcct
     6061 ctttgacttt taaaacaata aactaaaata aaatactgta catataacca ctggttttat
     6121 atacagtata aaaattacgc tgccttgttt ttcatgtcaa gtctgtaaat ttgcgttttt
     6181 gtgaaaatga tcaaaaaaac agccactcac accatgcgca atcccctcca gatgcaacaa
     6241 aaaccagcac aaacggcgcc gttctccggc ctcagtatgt tttactgaaa tcattatatg
     6301 agataccaga aagccccctc cgggacagga aaagaaacgg ggggtctggt tcgggttgtt
     6361 cggtcactga acacggtcag aaactgatga taagatagcc tgcggatata taactcaatg
     6421 agaaaaaaga attaaaggta aggagacgct catgagacgt tccggcagca aactgtgtct
     6481 tatcgccatg ataacagcga cagcaactct cactgcctgt gctccaaagg gcagcgtgga
     6541 acagcatacc cggcattacg tatatgcttc agatgacggt tttgatccca atttttccac
     6601 ccagaaagct gacacaacac gaatgatggt gccttttttc cggcagttct gggatatggg
     6661 cgcaaaggat aaagcgaccg gaaaatcacg gagtgatgtg cagcaacgca ttcagcagtt
     6721 tcacagtcag gaatttttaa actcactcca aggcacaaca caatttgcgg gtaccgatta
     6781 ccgcagcaaa gacctcacac cgaaaaaatc aaggctactg gctgacacca tttcagctgt
     6841 ttatctcgat ggctatgaag gcagacagta agtggattta ccataacgcg tcattacgcg
     6901 caccgctcag acgggctcag ggcgtttccg gcggctacgg gataaaacag gcaacaaccc
     6961 ccccggaaaa ctgccggaaa cgcgcccgat aaattttatc ctaatgcatg cctatgcacg
     7021 caactgcatg gcatatgttt tgacgtaaaa tggatgccgt aagccctttt ttaccgactt
     7081 tctgcttcat cagcctgact gacacgacca tattctttca gaccgccccc tgctaaactt
     7141 ccttcatatt gccgcattaa cccgggaggc tccgcaatga gcaacactac acactacgaa
     7201 aacgccaact ttcttcggga actggctgaa agcctgcccc gcatactgcc ggaaggtggc
     7261 ccggacaaag ctgcgctgtt gcagcgactg gctaacgaag aactggccca ggcagagtat
     7321 gaagatcagg ttcgcgccaa agtgaccgcc gcccgtgctg acacgcgtcc gggtatgacc
     7381 acggaacaac tccgccagcg cctgcatggc cgttatcagg aactgcgcga tgccgtatga
     7441 cgtggaatgg aagcagggag ccatcgagga tgtaacgacg ctgtttgact atatcgcgga
     7501 aaattcatcc ctgtgggatg cgcagaacgt taccgaccgg gttctcgctg cggcggacaa
     7561 actggccgac ttcccgcgac tctatgaagt ggactcacgc tatggcgagg acgttcgccg
     7621 gatcagtctg atgggacagc atgtcctcta tgatgttgat gaccagacca gaaaagtccg
     7681 ggttctggcc gtggtcggcc agcgccagaa tccccatatt gttctctaat aaccggtgac
     7741 ggggacgcaa ttcattgcgt ggccgtcgcc ggttggacaa gcacagcgcg tcagcgtttt
     7801 ccccacggcc accaccggcg cggtgttccg gccggggtct ctgctgtctc cggtttgtgt
     7861 tccagcagtc tcatggactg catttcctgc cgtagcccgg ccacctcctc cctgagtgct
     7921 tttatctccg cgatcaacgc gtcggaggcc attttttcag tctcacccga cagtctcaac
     7981 tcgtcccgct cgtgagtctc atcctgtctt aactcaccgt acacccggat aagctcgctg
     8041 gtatccacca cccggccgcc atcagcttcc gtacggtatg atacgcggcc tttcgccata
     8101 tcccgataca gggaacttcg ggctttaccc gtcagttccg tcgcctgtct caccgtcacc
     8161 cacgccattg tctcaccccg ctatttttga gacagtatac ctgacaggcg cacaccatgc
     8221 ccggaaggaa agaatcatga caaaacagga aaaaacagcc ctcaatatgg ctaaatttat
     8281 taaggagcag tcgctgctat tgctcgataa actgaatgag ctggatatgg ataccccggc
     8341 cgacatgtgc gagcggctac acgaggatgc ggaaacactc tggcaggtga tgcgggataa
     8401 gctcggggag gaataacagc accggcatgg ccggtgctgg catttatttt ttgccgaacg
     8461 gccaccaccg gcgggcaggt ttgtcctccg gcagttcagg taccggcacg ttaaaatagc
     8521 ggctgttata ttcccgcatc agctccgctt tcagcttctc gttcaccgcg ctttccccgt
     8581 gccgctcact caccacgtca atcatcgcgt taatctgctc cggtgagcac ttcccggcat
     8641 caaccacctg cacaccgttc tcgatggcga accgctccac cttgcgatag aggtaatcac
     8701 acagcttcat tcctgtgggt ttgttccgtt cccattgcgg atttttacca gtgaaaaagt
     8761 acgtggcttt ttcgtcgctg ttgagccgct cgtcatacat cccgatggat ttcatcatcg
     8821 ccagatgctg ggcccggtcg acaaaacaga aaacatacgc cccggtctgc caggcgagca
     8881 ccaccatatc ttccagatgc tcgctggtcg gcatgatgca gcgctcgccg ttccggtcct
     8941 ccgcgtcttc aataaatacc atgtacagcg ccataatccc tcactccgtt gtcataaatt
     9001 taaagcacgc aaacaaccgt tcacgatcca gctctgccgt tttgcgggcc tcgccggtct
     9061 gtgggttcac gatttcatcc accgacagga ttgcccgctg caccctgacc tgcgtcgccc
     9121 ggtccgggca gatgtaccag atttcatcga tgccgttagc tttgcggttg aacagatacc
     9181 cggacaccac ttcctgatac cggcgacggc tcttgacggt gcgctccacc tcaaacgcca
     9241 ccgttttacc gtccagctcc gccagggcgt ccggcacttt catgccttta agactcagac
     9301 ggtgcagaat gcgccagccg gtggcccctc tggcttccat atcaaggcgc agggattgca
     9361 cggccagccg gtgcggcaga tgtgctgccg acacccttga cggctgaaac ggaataacat
     9421 ccgccagcgg ttcgtcctcg tcgaacgaga acgccacgcc atgcggcgtc agcccccaca
     9481 cgctgacctg acggccgtcc accgga
//
