LOCUS       NZ_OP378620             4082 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Escherichia coli strain EC00661 plasmid pEC00661_3, complete
            sequence.
ACCESSION   NZ_OP378620
VERSION     NZ_OP378620.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Empirical genomic methods for tracking plasmid spread among
            healthcare-associated bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2022) Medicine/Infectious Diseases, University of
            Pittsburgh, 3550 Terrace Street, Scaife Hall S853, Pittsburgh, PA
            15213, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Sequencing Technology        :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028737885.1-RS_2024_03_26
            Annotation Date              :: 03/26/2024 01:23:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP378620.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC00661"
                     /isolation_source="Clinical"
                     /db_xref="taxon:562"
                     /plasmid="pEC00661_3"
     gene            join(3785..4082,1..404)
                     /locus_tag="PWB81_RS00005"
     CDS             join(3785..4082,1..404)
                     /locus_tag="PWB81_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(660..2819)
                     /gene="mobQ"
                     /locus_tag="PWB81_RS00010"
     CDS             complement(660..2819)
                     /gene="mobQ"
                     /locus_tag="PWB81_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            2998..3252
                     /gene="mobC"
                     /locus_tag="PWB81_RS00015"
     CDS             2998..3252
                     /gene="mobC"
                     /locus_tag="PWB81_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2462..2820)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2821..2997
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          61..404
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.862"
                     /coverage="100.0"
                     /evalue="4.27e-87"
     Typing          3786..4082
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.96e-154"
     Typing          complement(664..2906)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcgggcaca gtacgctaaa ggcgaggcca gagcacaaat aacattttct gacgcagtaa
       61 tgccttattt gacacaacta caagggcaat ttactagagt tgtaatcaag aatatatcta
      121 gattatcaag aagttacagt ataaggatat acgaactcct ccagcagttc cgcagtactg
      181 gcgaacgtat tatcgctctc gatgattttc gctcaatgct tgatatagag cataaatatc
      241 aaacatataa atcacttaac cagcaattat taagaccctg cattgacgaa ctgaacaaaa
      301 aaagcgacct ggctgtaacg gttgagacga tcaaaaaggg gcgcacggtt gtagccctgc
      361 attttcgctt caaggaagac aaacaaatca agatgacgat ctaaaatcga ttttaaggca
      421 cctaaaacgc tccctgctgc gttttttttg tgttagtagt gcgaaggtac tattgaagtg
      481 aaaaattgcg tcaaaagcgc tctaatgcag cgaaaaaaga acccccattt tttgggggcg
      541 gaaaaacaaa actatgcttg cattatgtgt gtgcttcttg cattcatccc tgtcgatgaa
      601 catcgaagaa catcgacgaa cataacacga tgttaaacaa aacaaaatat tgttcacatt
      661 cactgtgacc aaccgcgatc atgtctcctc tcggtggcgt gtgttcgtgc atcttcctcg
      721 cgccgccatt cgtcccaggt acgattaaaa cccagcgagc ggaacagatg atccggctgt
      781 ttttctggtg tgttgacggc agcaaaaagc ggtgcctcac gggttttcag atgaaactcc
      841 ataagttccg gcgcaggtga tatgtggctg tctgcatcgt gatttacggc aacaccatac
      901 cctgcatacc gtaacccctt atcggtcagt gcactgatga ccagctggct gttatcggct
      961 gagggaacgg caaacgcggg atacagcgta ccattaagcc ggacaacacc gcccagagag
     1021 tcatccacgc ccatgaccag caaaagctga agctggcggg tttcccggtc gacagaactc
     1081 attaccggca tcagcgtcgg tagcagacgt ccctgcgtaa aatccacctt ccttgctgcc
     1141 agtgatggca tggctggctg gcggtcttct ccaagacaga aagactccgg ccatgtcttc
     1201 ggctggcgga gattgcgggt atattgcggc catgtctggc aaacctgcgt cagaagttgc
     1261 tcaaaggtcg cagaggaata aacacgttgc agtggtggat gttgaccgat ttcgcttcgg
     1321 tcacagatat aaccactcct gtcacgcatt aaatcaacac gctcaaagat tctggtgcgg
     1381 tatttctcca gctcgttccc ccagagcgtg tattctccgt tccggttgtg aagcagaagg
     1441 taacaggtca tttccccgtt accaggcatg ggggcctcgc cataccccgt aacgactgcg
     1501 ctcagtaccc ctgaatccat aacggcattg tcatttttcc tgagctgttc cgctcgtcgc
     1561 tccgcgcgac tacgttctgt atcctcctgc tccttccggc gcaattcctc ttcacgttta
     1621 agcaactgga ctctgagagc tgcaaggccg gatgcccccc actgctgcca ctgtttttca
     1681 aatgccgtct gactcgcggg aatgtcctgt ttctccacct tccggtaatg ctgtcgcaat
     1741 ttttccattt gctggctcag cgactggatt tttccctgtt cgctacggta ttccggtgcc
     1801 gcccattgca tcaggggaat acgggaaaaa ccggacgaaa acggcgatga ttttttcagg
     1861 ctggcagcat ggctcatcgc ttcctttcgt tcggtgttca gctcttccca gcgtctgcgc
     1921 gtcgcctgca tatctgccat catcgacatg gcattactgg tgagtttcca tgccgcatta
     1981 ctgtccttcg gcaacgcttc gatgatgtca cgtagtgtgt acgaagttgc gttgtcagga
     2041 agcacgatat tcagaaggtc agccacacgg cgggcaggct ctgtcagccc gctgtttctg
     2101 agttcggtac gggaaatctg tatttccagg ttaagtctgt taatcagacg attctgttcg
     2161 ccgcgttctg tttcgatacc gcgtgtttcc atcgcacagg cagactttcc gaggtgaata
     2221 ccaggcgttt tctcaagccc ctgtcgctcg tatgaacgat ggtctatctc ttcctgataa
     2281 ccggcgttcg ccagtgccct gttcgcatgg tcagcccatg atgcacgcca cgtctcggca
     2341 tgtgtccggt cgttccagtc cctgaccttt ccaccgaatc cagcaggtcc gacagctctc
     2401 gtggtcagca tgatgtgcgc atggggattt gttttatcca tgtgatggaa cgccacatca
     2461 gcaatcatgc cctgactgac aaagttttct ctgacaaagg cgagaacggt ttcccgtgcg
     2521 gcatcctgcg gtagttcgcg ggggatggcg agttcaattt cccgcgcaag ttgtgagttc
     2581 ttccggcgtt cggccttctc aacggcattc cagagttcgg cacggttcgt acaccaggca
     2641 ggggctgtcg cgggagtcag aatcacagct tcagccacac cgcttttgcg ggtgtagtcg
     2701 tgagtcagtc cggtacgctc atcaagcatc agtgaaccgg aacgataggc agcagaagca
     2761 acggcgctgt agccgctgtt tctggagatg attttcatgc tgagatgata aatcgccatt
     2821 tctgccgtaa tgcctttttg tggttttctt tcagccccgc agggcgcaca caatcgaaac
     2881 ggagtggaga ttgtataagt gcgcacttcg tgattttccg tgcattatgc cagctcagtt
     2941 tttaaactac cagtgacgcg ctaaaatgcg atttttcatc gcgcacggag ttacatcatg
     3001 agcagaacac tcgaacagaa gattgctgat gctgaggcca gattacagcg cctgaaggcg
     3061 aagagcagga gtctggacac agcgcagaag gtcattgtgg gtgcagcatt gctggcaaag
     3121 gtcagaaagc cggaggaggt gcagttgcgt gcctggttac tccagttcct gaaggcagag
     3181 gtgacacgac aggctgatgt gacgcgcata ctgccgctga ttaacgagct ggaagcgctg
     3241 ccaggacagt gattgtatcg tccgctcgcc gcagtcccag cgaccgagcg ttagcgagcg
     3301 agtgggcgag gaagcggaag tgaatccgtc gcctgattga gcacatacgc acggtttttc
     3361 gggaggtgat atgcgctgaa cccgcatgaa tactggattt ccacagggtt ggagggggtg
     3421 tattcggaac ttgcaattct gcttttttgc aacgcattgt ttttattggt tttttgtttt
     3481 cagcactcca agttatccac aggctcgtcg ttaagttttt agatcttgtt tttaagatct
     3541 tgtttttaat acttgtttta gcgccaaaat tccttataga aaacaaaatg ttacaatgct
     3601 ataggtaatg tgatttaagg taaaggtaat atgatttaag gtgtttaggt gggggaattt
     3661 aaggtaaagg caatatgatt taaggtaata ttcacagaaa aagggatatt aaaataaaaa
     3721 ggcattgcct ttcgttgagg gtatgtttat cttttattca ttaccttttg gggagggatg
     3781 ctctatgtcg gatctaattg cctataaatc caatgcgctg gttgaagcca gttataaatt
     3841 gaccctacag gaacagcgat ttttgttact ttgcatcggt cgattgaagt ctggggctga
     3901 tgcagaatct ccaaaattgc aaaagacaat gaccattaca gctgcggaat atttcgattc
     3961 attccctgac atggggcgga aaaatgccga ggttcagtta caggaagcaa ttgatcgttt
     4021 gtgggacagg tcaatcattc tcaaggatga cgagaaacgt gaggagttcc gctggatcca
     4081 gt
//
