LOCUS       NZ_OP378617             4088 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Escherichia coli strain EC00609 plasmid pEC00609_5, complete
            sequence.
ACCESSION   NZ_OP378617
VERSION     NZ_OP378617.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Empirical genomic methods for tracking plasmid spread among
            healthcare-associated bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2022) Medicine/Infectious Diseases, University of
            Pittsburgh, 3550 Terrace Street, Scaife Hall S853, Pittsburgh, PA
            15213, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Sequencing Technology        :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028737855.1-RS_2024_03_26
            Annotation Date              :: 03/26/2024 01:16:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP378617.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC00609"
                     /isolation_source="Clinical"
                     /db_xref="taxon:562"
                     /plasmid="pEC00609_5"
     gene            join(2701..4088,1..772)
                     /gene="mobQ"
                     /locus_tag="PWB94_RS00005"
     CDS             join(2701..4088,1..772)
                     /gene="mobQ"
                     /locus_tag="PWB94_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029344.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALVNAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1028..1735)
                     /locus_tag="PWB94_RS00010"
     CDS             complement(1028..1735)
                     /locus_tag="PWB94_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2268..2522)
                     /gene="mobC"
                     /locus_tag="PWB94_RS00015"
     CDS             complement(2268..2522)
                     /gene="mobC"
                     /locus_tag="PWB94_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2524..2700)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2701..3059
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1030..1735)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.859"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3..769
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.11e-174"
     Typing          2615..4086
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcacaaccgg aacggagaat acacgctctg ggggaacgag ctggagaaat accgcaccag
       61 aatctttgag cgtgttgatt taatgcgtga caggagtggt tatatctgtg accgaagcga
      121 aatcggtcaa catccaccac tgcaacgtgt ttattcctct gcgacctttg agcaacttct
      181 gacgcaggtt tgccagacat ggccgcaata tacccgcaat ctccgccagc cgaagacatg
      241 gccggagtct ttctgtcttg gagaagaccg ccagccagcc atgccatcac tggcagcaag
      301 gaaggtggat tttacgcagg gacgtctgct accgacgctg atgccggtaa tgagttctgt
      361 cgaccgggaa acccgccagc ttcagctttt gctggtcatg ggcgtggatg actctctggg
      421 cggtgttgtc cggcttaatg gtacgctgta tcccgcgttt gccgttccct cagccgataa
      481 cagccagctg gtcatcagtg cactgaccga taaggggtta cggtatgcag ggtatggtgt
      541 tgccgtaaat cacgatgcag acagccacat atcacctgcg ccggaactta tggagtttca
      601 tctgaaaacc cgtgaggcac cgctttttgc tgccgtcaac acaccagaaa aacagccgga
      661 tcatctgttc cgctcgctgg gttttaatcg tacctgggac gaatggcggc gcgaggaaga
      721 tgcacgaaca cacgccaccg agaggagaca tgatcgcggt tggtcacagt gaatgtgaac
      781 aatattttgt tttgtttaac atcgtgttat gttcgtcgat gttcttcgat gttcatcgac
      841 agggatgaat gcaagaagca cacacataat gcaagcatag ttttgttttt ccgcccccaa
      901 aaaatggggg ttcttttttc gctgcattag agcgcttttg acgcaatttt tcacttcaat
      961 agtaccttcg cactactaac acaaaaaaaa cgcagcaggg agcgttttag gtgccttaaa
     1021 atcgatttta gatcgtcatc ttgatttgtt tgtcttcctt gaagcgaaaa tgcagggcta
     1081 caaccgtgcg cccctttttg atcgtctcaa ccgttacagc caggtcgctt tttttgttca
     1141 gttcgtcaac acagggtttt attagatccc tgtttaacgt tttgaaatct ttatatttac
     1201 catcaagcat cagtgatgat ttaaaatcat cgagggcgat aatacgttcc ccagtactgc
     1261 ggaattgctg gagaatttcg tatattctta tgctgtaact tcttgataaa ttagaaatat
     1321 ttttaatgac gactctggtg aattgcccct tcaactgtgt cagataaggc attaccgcgt
     1381 cagaaaaagt gatttgtgct ttcccctctc ccctcgcgta ctgtgcccga tactgaatcc
     1441 agcggaactc ctcgcgcttc tcgtcgtcct tgagaatgat cgacctgtcc cacaaacgat
     1501 cgatcgcttc ctgtagctga acctcggcat ttttccgccc catatcaggg aatgaatcaa
     1561 aatattccgc agctgtaatg gtcattgtct tttgcaattc aggtgaagct acgtcagtac
     1621 cggagttaag tcggcttatg cagagcaata agaaacgctg ttcttgtaaa gttagcttgt
     1681 aactggcctc aaccagcgca ttggacttat aggctatcag agggtttgat gtcattttaa
     1741 ccaccaggcc aaaaatgagg tgaaataaaa acctatcacc tttagcattg gtgagcaatt
     1801 atattttcac cccccatttt cctgtgaata ttaccttaaa tctcctcacc tttaccttaa
     1861 attacctcac ccaagcacct taaattatat cacctttacc ttaaatcata ttacctatag
     1921 cattgtaaca ttttgttttc tataaggaat tttggcgcta aaacaagtat taaaaacaag
     1981 atcttaaaaa caagatctaa aaacttaacg acgagcctgt ggataacttg gagtgctgaa
     2041 aacaaaaaac caataaaaac aatgcgttgc aaaaaaagca gaattgcaag ttccgaatac
     2101 accccctcca accctgtgga aatccagtat tcatgcgggt tcagcgcata tcacctcccg
     2161 aaaaaccgtg cgtatgtgct caatcaggcg acggattcac ttccgcttcc tcgcccactc
     2221 gctcgctaac gctcggtcgc tgggactgcg gcgagcggac gatacaatca ctgtcctggc
     2281 agcgcttcca gctcgttaat cagcggcagt atgcgcgtca catcagcctg tcgtgtcacc
     2341 tctgccttca ggaactggag taaccaggca cgcaactgca cctcctccgg ctttctgacc
     2401 tttgccagca atgctgcacc cacaatgacc ttctgcgctg tgtccagact cctgctcttc
     2461 gccttcaggc gctgtaatct ggcctcagca tcagcaatct tctgttcgag tgttctgctc
     2521 atgatgtaac tccgtgcgcg atgaaaaatc gcattttagc gcgtcactgg tagtttaaaa
     2581 actgagctgg cataatgcac ggaaaatcac gaagtgcgca cttatacaat ctccactccg
     2641 tttcgattgt gtgcgccctg cggggctgaa agaaaaccac aaaaaggcat tacggcagaa
     2701 atggcgattt atcatctcag catgaaaatc atctccagaa acagcggcta cagcgccgtt
     2761 gcttctgctg cctatcgttc cggttcactg atgcttgatg agcgtaccgg actgactcac
     2821 gactacaccc gcaaaagcgg tgtggctgaa gctgtgattc tgactcccgc gacagcccct
     2881 gcctggtgta cgaaccgtgc cgaactctgg aatgccgttg agaaggccga acgccggaag
     2941 aactcacaac ttgcgcggga aattgaactc gccatccccc gcgaactacc gcaggatgcc
     3001 gcacgggaaa ccgttctcgc ctttgtcaga gaaaactttg tcagtcaggg catgattgct
     3061 gatgtggcgt tccatcacat ggataaaaca aatccccatg cgcacatcat gctgaccacg
     3121 agagctgtcg gacctgctgg attcggtgga aaggtcaggg actggaacga ccggacacat
     3181 gccgagacgt ggcgtgcatc atgggctgac catgcgaaca gggcactggt gaacgccggt
     3241 tatcaggaag agatagacca tcgttcatac gagcgacagg ggcttgagaa aacgcctggt
     3301 attcacctcg gaaagtctgc ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc
     3361 gaacagaatc gtctgattaa cagacttaac ctggaaatac agatttcccg taccgaactc
     3421 agaaacagcg ggctgacaga gcctgcccgc cgtgtggctg accttctgaa tatcgtgctt
     3481 cctgacaacg caacttcgta cacactacgt gacatcatcg aagcgttgcc gaaggacagt
     3541 aatgcggcat ggaaactcac cagtaatgcc atgtcgatga tggcagatat gcaggcgacg
     3601 cgcagacgct gggaagagct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc
     3661 ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta ttcccctgat gcaatgggcg
     3721 gcaccggaat accgtagcga acagggaaaa atccagtcgc tgagccagca aatggaaaaa
     3781 ttgcgacagc attaccggaa ggtggagaaa caggacattc ccgcgagtca gacggcattt
     3841 gaaaaacagt ggcagcagtg gggggcatcc ggccttgcag ctctcagagt ccagttgctt
     3901 aaacgtgaag aggaattgcg ccggaaggag caggaggata cagaacgtag tcgcgcggag
     3961 cgacgagcgg aacagctcag gaaaaatgac aatgccgtta tggattcagg ggtactgagc
     4021 gcagtcgtta cggggtatgg cgaggccccc atgcctggta acggggaaat gacctgttac
     4081 cttctgct
//
