LOCUS       NZ_OP378615             6200 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Escherichia coli strain EC00609 plasmid pEC00609_3, complete
            sequence.
ACCESSION   NZ_OP378615
VERSION     NZ_OP378615.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Empirical genomic methods for tracking plasmid spread among
            healthcare-associated bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2022) Medicine/Infectious Diseases, University of
            Pittsburgh, 3550 Terrace Street, Scaife Hall S853, Pittsburgh, PA
            15213, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Sequencing Technology             :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028737835.1-RS_2024_03_26
            Annotation Date                   :: 03/26/2024 01:20:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP378615.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC00609"
                     /isolation_source="Clinical"
                     /db_xref="taxon:562"
                     /plasmid="pEC00609_3"
     gene            47..469
                     /locus_tag="PWB69_RS00005"
     CDS             47..469
                     /locus_tag="PWB69_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            513..>917
                     /locus_tag="PWB69_RS00010"
                     /pseudo=""
     CDS             513..>917
                     /locus_tag="PWB69_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(1024..1860)
                     /locus_tag="PWB69_RS00015"
     CDS             complement(1024..1860)
                     /locus_tag="PWB69_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(1860..2663)
                     /gene="aph(3'')-Ib"
                     /locus_tag="PWB69_RS00020"
     ARG             complement(1864..2663)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2724..3539)
                     /gene="sul2"
                     /locus_tag="PWB69_RS00025"
     ARG             complement(2728..3539)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3999..4157)
                     /locus_tag="PWB69_RS00040"
     CDS             complement(3999..4157)
                     /locus_tag="PWB69_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            4728..4877
                     /locus_tag="PWB69_RS00030"
     CDS             4728..4877
                     /locus_tag="PWB69_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(5622..6062)
                     /locus_tag="PWB69_RS00035"
     CDS             complement(5622..6062)
                     /locus_tag="PWB69_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     ARG             complement(1028..1860)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          4520..4693
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(3991..4596)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agtatttcga atactgtata taaacacatt gttataaata cagtttttgc tgtttttaaa
       61 agtcaaagag gataatttta tgagtacaga gttaaatagc ggtactgtcg gtggttttgg
      121 tcacatggga ccaaatgacc cctacaatga tggtcatagc ggctctggaa gttcgggagg
      181 tagttttaac ggtggtggta atggtggcca tgataatagt agtggttcat cttatactgc
      241 atcctttggt ctcatcactg gccgacaccc gataactgtc ccaggtacga agaatattaa
      301 ttctgtcgat ctttctgctg tcattaataa cattatacat catgaaggtg cacgcaataa
      361 catgtaccta gatactgcga acaatgtaag agtgggagtg acgggcactg gctggcaatg
      421 tctagcaacg gcaggcattt cggctgaggg taaaagaact ttccgctaag cgatagactg
      481 tatgtaaaca cagtattgca aggacgcgga acatgcctca tgtggcggcc aggacggcca
      541 gccgggatcg ggatactggt cgttaccaga gccaccgacc cgagcaaacc cttctctatc
      601 agatcgttga cgagtattac ccggcattcg ctgcgcttat ggcagagcag ggaaaggaat
      661 tgccgggcta tgtgcaacgg gaatttgaag aatttctcca atgcgggcgg ctggagcatg
      721 gctttctacg ggttcgctgc gagtcttgcc acgccgagca cctggtcgct ttcagctgta
      781 agcgtcgcgg tttctgcccg agctgtgggg cgcggcggat ggccgaaagt gccgccttgc
      841 tggttgatga agtactgcct gaacaaccca tgcgtcagtg ggtgttgagc ttcccgtttc
      901 agctgcgttt cctgtttggg gtcgtttgcg ggaaggggcg gaatcctacg ctaaggcttt
      961 ggccagcgat attctccggt gagattgatg tgttcccagg ggataggaga agtcgcttga
     1021 tatctagtat gacgtctgtc gcacctgctt gatcgcggcc gcgatagcta gatcgcgttg
     1081 ctcctcttct ccatccgcgt tccaagctgc ggaaaggcac ccataagcgt acgcctggtc
     1141 gagcaggcga cgcggatcga cgtccagcgc acgagagaat gcgtccgcca tctgtgcaat
     1201 gcgtctagga tcgagacaaa ggtcgtctct gtcagccgga tcgtagaaca tattggcggc
     1261 gccaaagccc acttcaccga ccagaccgac gggatctatc accagccagc cgcgactgga
     1321 gaacatgatg ttttcatgat gcagatcgcc atgtagccca cgcagttccg aggcattgct
     1381 catcatttga tcggctataa tcgccgcgtg gacgtagtca gtttgacaac ctgcgttttg
     1441 atcatcgcgc gcccgctgaa acaaagctgc aaagcgatcc cggatcggga gaagggcaga
     1501 aggcaggggt tcctcagatg cggcatacag cttcgccatt agttccgctg caatttcggt
     1561 cgcctggtag tcgccgtgct cggcaacgat gtgagagagc attcgctccc cggcatattc
     1621 gagcaacatc agattgttct cacgaccgag caaccggact gctcccctcc cattgcgcca
     1681 taccagatag tcggccccgc gcagttcatc agcaatgtct tctataggtt tcaatccctt
     1741 gacgattgca ggagtcccgt ctggcaatga aactttccaa acgaggctgg aaaaggtgtc
     1801 cgcaatgaga acaggttgcg aaacgtgcca atgagcagga aaaacaggcg gcatgaacat
     1861 caaccccaag tcagagggtc caatcgcaga tagaaggcaa ggcgttcgcg gtcgggggct
     1921 tcgatcccca atacattgaa taggacagcg aaggcgcgct ctgcttcatc tggcgctgcc
     1981 cagttctctt cggcgttagc aatcatgagt gccaaatcgg catagcgatc tgctgttccg
     2041 agccgcccaa ggtcgatcag acccgtgcat tgaagagttt tagggtccac catgaagttc
     2101 ggcatgcagg gatcaccatg gcaaacaacc atatcggtgc gctcttggtc gagccgcacc
     2161 ggtagctctc gttcgacacg agccaaaaga tcgagctgcg gcgtactctt gtcctcgtcc
     2221 ggtaagaagt cgggattgac ggcattgcgg gacaccacat caacggcgcg tccgaacatt
     2281 cgcgacagcc tgcgctcaaa cggacattga tcaaccgata ggctgtgaac agcgccaagt
     2341 tgctgcccca ttgacggcca cgctttgagc aaatccgctc cagacagatc agccgccggt
     2401 actcccggaa ttgccgttat caccaagcat gcaccctcct gttcctcctg ccagttgatg
     2461 acctcggggc aagccacacc tcgacctttg agccaaatga ggcggtcacg ctctccagcg
     2521 agctcaccgc ggcgggaagc aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga
     2581 aaaacaaaat caccagattc tccgcctctg acaggcaacc agtcagaatg cgattcacca
     2641 aaaaaaatat tagttcgatt caatggaggt tccttcagtt ttctgatgaa gcgcgaatat
     2701 agagaaatat cccgaatgtg cagttaacga attcttgcgg tttctttcag cgccgccaat
     2761 accgccagcc cgtcgcgcaa ggggcgcggc tcgtgtgtgc ggatgaagtc agctccacct
     2821 gcggcggcgg caagctctgc agcgagtgtc gcggccccga catcccccgg accacggcct
     2881 gtgagcgcgc gcagaaagga tttgcgcgaa acagacagaa gcaccggcaa atcgaagcgc
     2941 agccgcaatt catcgaaccg cgccagcacc gagagcgagg tttcgggagc agcccccaga
     3001 aaaaacccca tgccgggatc aaggacaagg cggttgcgtt tgataccggc acccgtcagc
     3061 gccgcgatgc gcgcgtcaaa gaacgccgca atgtgatcca tgatgtcgcc agcgggtgcc
     3121 tcgcgccgat ctgcctgccc gtcttgcacc gaatgcataa cgacgagttt ggcagatgat
     3181 ttcgccaatt gcggatagaa cgcagcgtct ggaaaaccgc gaatatcatt gagataggcc
     3241 acaccacgcg acaaggcata ggcttgcgtc gcgggttgat aactgtcgag cgagacggga
     3301 atgccatctg ccttgagcgc gtccagcacc ggcgcgatac gcgcgatttc tgtgtcggac
     3361 gaaacaggcg cggcgtcggg attgctggat gccggaccga ggtcgatcac atctgccccc
     3421 tcggccatca gcttacgcgc ctgcgcaatg gctgcgtctg gcgccagata ccggcctcca
     3481 tcggagaaac tgtccgaggt tatgttgacg atgccgaaaa tgatgagcga tttattcatg
     3541 ggggcttcta taataataat aatcgagcat gagtctcata cggatgctcg ggtcgaaagg
     3601 gaatccccag gcgagtaacc tgtttgcggt gatccgtttc atgatgatgt atctgtagca
     3661 ttttggaaat aaatatatca tctctcaata agtagatata tttatctttt ttgcttcttt
     3721 ggtgctgcaa gtcaccaata cgagggaaac ctgttacgga gcggtttaac cgtaatcaac
     3781 agtgactggt gtcatgttcg gaagattaat tgatagtaat ctacaaagga tcttcttgag
     3841 atccttttca atgaatcgta atctcttgct ctgtaaacga aaaaaccgcc tggggaggcg
     3901 gtttgttcga aggttaagtc agttggggaa ctgcttaacc cggtaacagg gttcacgaga
     3961 ccgccattac caaaactgtt ctttcagtgt agccgcagtc aggcctctac ttcaagacct
     4021 cttaatctat cttctcgtgc actcctgtta ctaatggctg ctgccagtgg cgttttgtcg
     4081 tgtctttccg ggttggactc aagttgatag ttaccggaca aggcgcagcg gtcggactga
     4141 acggggggtt cgtgcataca gtccagcttg gagcgaactg ccttcacgga actgagtgtc
     4201 aggcgtggaa tgagaaaccg cggccataac agcggaatga caccggtaaa ccgaaaggca
     4261 ggaacaggag agcgcacgag ggagctacca gggggaaacg cctgggatct ttatagtcct
     4321 gtcgggtttc gccaccactg atttgagcgt cagattttgc gatgcttgtc aggggggcgg
     4381 agcctatgga aaaacggctt cgccgtggcc ttatctcttc tttgctaagt gtcttcctgg
     4441 tatcttccag gaaatttccg tccccgtccg taagccggta ccgctcgccg cagccgaacg
     4501 accgagcgta gcgagtcagt gagcgaggaa gcggaatatc tcctgtatcg catattctgc
     4561 tgacgcgccg gtgctgcctt ttttctcctg ccacatgaag cacttcactg attttcgcaa
     4621 tcgtggcaac atagtaagcc agtatacact ccgctagcgc tacatgactg gttcagggct
     4681 gcgccccgaa acccgctaac aacctctcag ctactggata tcaccccatg ccatctgaat
     4741 atgatgaaca actggattcg actgatacgc agcttcccgg cgaatatgac ctttttgatt
     4801 taatggcgca ggttactgaa gagaacctgc atgcccctgt ggattttggg tcgcctgtcg
     4861 gccgggaagc gttatagctt tttcgcttgc ccgcgtacgt ggtaatactc cggtattacc
     4921 ttttatttct ttattttcat gtaattacag tggattttta atacccgggt attactcgtg
     4981 aaaacgaaat attccttttt tttcttttaa ttcatgcagt tgcgatgaaa atcgcggctg
     5041 cgttaggtgt atgaccattt tgaggttgaa aaataatcaa ccatggcggg tgatttacgc
     5101 aggttaagga gagatcgtga caaaaaaagc gttcctcact gtatcgttca tgcctgcatg
     5161 aacgggttat tcttcctgac tacagaatta catcgctcct tcagttcata catcactttt
     5221 acatcactta tacatcactt ttacatcatt ccttcagctc atacgtcact ttcatcctct
     5281 gtgccatttg tacatcaccc ccttcctgaa cgtgctgaca gcgggttttc cggctgcgcc
     5341 ggcaacgctt cacggccacg caataaattg cgtccccgtt caccgttttc ggatgggaag
     5401 aacgccggaa acaggactta ccccggctga atggtgtgaa tttccgcgct atgcacttgt
     5461 gcgcatactc atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg
     5521 gtttgttgcc tgttttaccg gtttcccgtc agaaacgccc tgagggcctc tcaggcggtg
     5581 ctgataacag atgttatggt aaatcagagt cacctgcact gttactctga accggagcga
     5641 aacagccgcg accagaatcc ccgctttttt tcctgtgcca gcgcgtcagc cagacggcta
     5701 atttcggcct gtagttgttc cctctcctca tttcgcaggc ggttttctgc ttcccgcgcc
     5761 atcttgtctt caagcattag ggtaacagtc tgctgcagag cgtccagttt ctgctgaatg
     5821 acgtctagct gagcattttc cgcctggcta acgacagatt tggagtgtcc ctccttgtgc
     5881 ctttttggtg tctcagaagt gcgcagctcg ccataggccc gaatcagttc actggtctcg
     5941 aactcccggc gatcattttt tcctgtgcga tagctgacaa ggcctttagc catgtcacga
     6001 tataattgcg agcgcgactt tcctgttaat ttcagtgtct cttgaactgt gtgccatgcc
     6061 ataaaagtga cagtgtctcc tgtgtgtacc gtattgtaac agcagaacat attactgttt
     6121 taacacaaaa ggtctgaggc tgcccgtgag gcgtgaaaaa tacacaaaaa acctgttgac
     6181 ggtgtggttt tgacgggcgt
//
