LOCUS       NZ_OP378606             9293 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Citrobacter koseri strain CB00073 plasmid pCB00073_2, complete
            sequence.
ACCESSION   NZ_OP378606
VERSION     NZ_OP378606.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Empirical genomic methods for tracking plasmid spread among
            healthcare-associated bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Evans,D., Sundermann,A., Griffith,M., Srinivasa,V., Mustapha,M.,
            Chen,J., Dubrawski,A., Cooper,V., Harrison,L. and Van Tyne,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2022) Medicine/Infectious Diseases, University of
            Pittsburgh, 3550 Terrace Street, Scaife Hall S853, Pittsburgh, PA
            15213, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Sequencing Technology        :: Illumina; MinION
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028737745.1-RS_2024_03_26
            Annotation Date              :: 03/26/2024 01:17:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP378606.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="CB00073"
                     /isolation_source="Clinical"
                     /db_xref="taxon:545"
                     /plasmid="pCB00073_2"
     gene            join(7797..9293,1..189)
                     /locus_tag="PWB66_RS00005"
     CDS             join(7797..9293,1..189)
                     /locus_tag="PWB66_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            199..456
                     /locus_tag="PWB66_RS00010"
     CDS             199..456
                     /locus_tag="PWB66_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            541..690
                     /locus_tag="PWB66_RS00015"
     CDS             541..690
                     /locus_tag="PWB66_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2171..4135
                     /locus_tag="PWB66_RS00020"
     CDS             2171..4135
                     /locus_tag="PWB66_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            4135..4866
                     /locus_tag="PWB66_RS00025"
     CDS             4135..4866
                     /locus_tag="PWB66_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            4873..5403
                     /locus_tag="PWB66_RS00030"
     CDS             4873..5403
                     /locus_tag="PWB66_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5431..5610)
                     /locus_tag="PWB66_RS00035"
     CDS             complement(5431..5610)
                     /locus_tag="PWB66_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5679..6056
                     /locus_tag="PWB66_RS00040"
     CDS             5679..6056
                     /locus_tag="PWB66_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(6118..6417)
                     /locus_tag="PWB66_RS00045"
     CDS             complement(6118..6417)
                     /locus_tag="PWB66_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6407..6670)
                     /locus_tag="PWB66_RS00050"
     CDS             complement(6407..6670)
                     /locus_tag="PWB66_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(6976..7395)
                     /locus_tag="PWB66_RS00055"
     CDS             complement(6976..7395)
                     /locus_tag="PWB66_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     BGC             2797..9293
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(794..1372)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4136..4863
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ggtggtggtg gtaagcgggc ccgatggtat ggtgataaaa agcgtaaaat ttatgaatgg
       61 gattcccagc acggtgagct tgaagggtac cgcgccagtg atggcgaaca cctcggggca
      121 ttcgatccaa aaacgggtaa gcaggttaaa gggccggatc caaaacgaaa cattaaaaaa
      181 tatctttaag aggtaaatat ggggcttaaa ttacatattc attggtttga taagaaaacc
      241 gaagagttta aaggtggtga atactcaaaa gacttcggtg atgatggttc tgtcattgaa
      301 agtctgggga tgcctttaaa ggataatatt aataatggtt ggtttgatgt tgaaaaatca
      361 tgggtttcga tattacagcc acactttaaa aatgtaatcg atattagtaa atttgattac
      421 tttgtatcct ttgtttaccg ggatggtaac tggtaagcaa aagttatcaa ggatgatttg
      481 aaatacagac ttccggtgtt cagggatgaa tgccggggtt tttaaataca gggaaggtat
      541 atgaaaaagg taaaggttgt ttttcttttt atattgattt tttcaggctt tcttctggtc
      601 gcatgtcagg caaactatat ccgggatgtt cagggtggaa cggtggcacc atcgtcctcc
      661 tctgaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt
      721 tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac cacctgggga ggtggtttga
      781 tcgaaggtta agtcagttgg ggaactgctt aaccgtggta actggctttc gcagagcaca
      841 gcaaccaaat ctgtccttcc agtgtagccg gactttggcg cacacttcaa gagcaaccgc
      901 gtgtttagct aaacaaatcc tctgcgaact cccagttacc aatggctgct gccagtggcg
      961 ttttgcgtgc ttttccgggt tggactcaag tgaacagtta ccggataagg cgcagcagtc
     1021 gggctgaacg gggggttctt gcttacagcc cagcttggag cgaacgacct acaccgagcc
     1081 gagataccag agtgtgagct atgagaaagc gccacacttc ccgcaaggga gaaaggcgga
     1141 acaggtatcc gggaaacggc agggttggaa caggagagcg caagagggag cgattcatcg
     1201 gaaacggtga tgatctttaa gtcctgtcgg gtttcgccac tcctgactga ttcatggttg
     1261 agccaccggc tcccacagat gcaccgaaaa agcgtctgtt tatgtgaact cagtcaggag
     1321 ggcggagcct atggaaaaac gccacctgcg tggccttgcc gttttgcctc acatgttagt
     1381 cccctgctta ttcacggaat ctgtgggtaa ctttgcatgt gtccgcagcg cccgccgcag
     1441 tctcacggcc ggagcgtagc gaccgagtga gcgaggaggc gcaatttccc cggcacctac
     1501 aagacggaca ccggacgcgc tgatgtggca tattgtcccc tgattaagtc tttatattcc
     1561 ctgataataa attaccagga taggtgaatc atatcggttt ttcgctgaac attttacgtt
     1621 aacctatctc tgttgctttt ttctccttac ggagaaatac aggtcatccc cgcgagggcg
     1681 tggctgacct cagggggata tcccctgctt acgctggctg gacgccaccg acgcacggtc
     1741 tctccccctc tttctggctc cgtccggctg gacgccggcc acgccagaaa ttcacccggc
     1801 ccccgtcagg ctcacgcctg accacccaca aaaatccacg gtgctggacg cacctggcga
     1861 agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc tcgctatggc gaactctgac
     1921 gggggccgga acaccggaaa agaccgcact ggggtgccgc cttttcctct cacacgctgg
     1981 ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca tggggcggaa agaggctggc
     2041 ctccgtggtg aaagctgaac gcaatcacca cggacgtgac cgccgccgcc atgccttggg
     2101 gcatgtccgc ggcttgtgca gcgggggctg cacatcacgc cggcaaacca tcgtctgaag
     2161 gagagagaac atgtttaata cggattcgct tgcctggaag tggcacattc cggagctggc
     2221 ccggcagatt tatccgtggg tcagctggct gtaccaggag tattacggtc tggccggggc
     2281 tttcctagtt atcggcctgt ttttcggtct ggctgaactt tgggccggac ggatgcgttt
     2341 tgtggggctg agtcataagg agcgacggga gtatgccgat cgctataagc gcgataataa
     2401 acggccagcg ccattttcgg tgctggcctc gctggattcg cccgggcgga tggtcagggc
     2461 acgtcgacat gtctggaact acgcgtactg gctgaccggc gcggccggtc tggcccttct
     2521 gggggggctg gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgtccgga
     2581 cctctacgca aacgcagatt attacctgct gcagccggct aacctgttcg ggattgcctg
     2641 gtgcgcgggc gggctggctg tcgggattgt gttggcgcgg ctgctggcgt tcctgctgct
     2701 tgacggtcat tttgcggctg ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg
     2761 gcgcagcagc cagcggacag gagagatgac cgacgtgcgc cacctgcatt tcggggagcc
     2821 ggtaccggtt aatgcgctgg ccgatttttc ctcggagcag gcccggcagg agcaggcggt
     2881 gtttctgggt aaggatgagc agggcaagcc ggtgctggtt ccgcgtgata cctggcgcaa
     2941 gaccaacatt cagatcctcg gcctgcccgg gagcggcaag agcgtgatgg ccaccaacgc
     3001 gctcattcgc tgcgtacgcg atttcgggga cgcggtggtg tacttcgacc ccaaagggga
     3061 cgcatgggcg ccgcacgtct tccgggcgca ctgtccggac ttcaccctgc tggatctgcg
     3121 gccggggaaa ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg cgctgaaaaa
     3181 cctgctgatt gccgggctta acctgggcga gaccggcgac gtggcggacc actaccgcat
     3241 cagcgagcag aaagcggcga agctgattgc ggagcagttt ccgcaggggg caaacattca
     3301 gcaggtgctg gcggcggcgt acgcgctgcc ggaggccctg aaaaaggacg tgaaggggtt
     3361 gatcactagg ctggaaaacg tcgctgacct gagcgtcctg cagacggaca gtggtatcga
     3421 tgtggcgggg attatcaacg gcggcggctg cctgtacgtt atcggctcga tggacgacga
     3481 ggcggtgatc cgtgtgcaaa aaatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg
     3541 ggacgagttc cgggagtggc cacacgtcag cgtgatgctc gatgaaatta aatatctgct
     3601 gtcgaagtat gtactgaacg cactggggac gctgcgcagc cgggactgca atctgttgct
     3661 cgcgcaccag tcgctgggcg atttcgggca gtgcgggcag gatctgcccg ctgaatttgt
     3721 gaaaaccacg gtactggata acacgccgat ccgctggttt taccgggcgg ccagcctgga
     3781 gtccgcgcag tgggcggcgg ggcagaccgg ggaaatcagg gtggacgtgg agcgccgccg
     3841 tgccagccgg gaggcgggga acgtggagca tatcagcggg gacagcttca tccagaagga
     3901 ggcgcggccg ctgtttgacg tgaacaccct gcaacacctg ccggacgggt tcgcggtcat
     3961 gaccgggctg ggtgtggccc ggatggcgtt cagcagtccg ctgcgggttg agcgccggga
     4021 aatcccgttg cagcgtttcc cggtgctggc gaaaaccgat cccctggcgg agttccggcc
     4081 cccggccgac ggcggcgggc cgaaagcgga tgatgatttt gcggggattt actgatggcg
     4141 ctggagctgt acaacgtcag ccacgcgaaa cgacaggcgc ggaacgcgga gaaaacccgg
     4201 ctgaccctgc gctggttgcg ggaagagctg tgttccacgg cggagctggt tgcgcggcgg
     4261 ctggggattg cggcggtgca gccggtctac cgcttcctcg acagcctggt ggcgaaaggg
     4321 ctgctggtcc ggaccaaata tccggtggac ggccgtcagg tcagcgtgtg ggggctgacg
     4381 ccgcatggcg tggcgttctc gttcgacgag gacgaaccgc tggcggatgt tattccgttt
     4441 cagccgtcaa gggtgtcggc agcacatctg ccgcaccggc tggccgtgca atccctgcgc
     4501 cttgatatgg aagccagagg ggccaccggc tggcgcattc tgcaccgtct gagtcttaaa
     4561 ggcatgaaag tgccggacgc cctggcggag ctggacggta aaacggtggc gtttgaggtg
     4621 gagcgcaccg tcaagagccg tcgccggtat caggaagtgg tgtccgggta tctgttcaac
     4681 cgcaaagcta acggcatcga tgaaatctgg tacatctgcc cggaccgggc gacgcaggtc
     4741 agggtgcagc gggcaatcct gtcggtggat gaaatcgtga acccacagac cggcgaggcc
     4801 cgcaaaacgg cagagctgga tcgtgaacgg ttgtttgcgt gctttaaatt tatgacaacg
     4861 gagtgaggga ttatggcgct gtacatggta tttattgaag acgcggagga ccggaacggc
     4921 gagcgctgca tcatgccgac cagcgagcat ctggaagata tggtggtgct cgcctggcag
     4981 accggggcgt atgttttctg ttttgtcgac cgggcccagc atctggcgat gatgaaatcc
     5041 atcgggatgt atgacgagcg gctcaacagc gacgaaaaag ccacgtactt tttcactggt
     5101 aaaaatccgc aatgggaacg gaacaaaccc acaggaatga agctgtgtga ttacctctat
     5161 cgcaaggtgg agcggttcgc catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc
     5221 tcaccggagc agattaacgc gatgattgac gtggtgagtg agcggcacgg ggaaagcgcg
     5281 gtgaacgaga agctgaaagc ggagctgatg cgggaatata acagccgcta ttttaacgtg
     5341 ccggtacctg aactgccgga ggacaaacct gcccgccggt ggtggccgtt cggcaaaaaa
     5401 taaatgccag caccggccat gccggtgctg ttattcctcc ccgagcttat cccgcatcac
     5461 ctgccagagt gtttccgcat cctcgtgtag ccgctcgcac atgtcggccg gggtatccat
     5521 atccagctcg ttcagtttat cgagcaatag cagcgactgc tccttaataa atttagccat
     5581 attgagggct gttttttcct gttttgtcat gattctttcc ttccgggcat ggtgtgcgcc
     5641 tgtcaggtat actgtctcaa aaatagcggg gtgagacaat ggcgtgggtg acggtgagac
     5701 aggcgacgga actgacgggt aaagcccgaa gttccctgta tcgggatatg gcgaaaggcc
     5761 gcgtatcata ccgtacggaa gctgatggcg gccgggtggt ggataccagc gagcttatcc
     5821 gggtgtacgg tgagttaaga caggatgaga ctcacgagcg ggacgagttg agactgtcgg
     5881 gtgagactga aaaaatggcc tccgacgcgt tgatcgcgga gataaaagca ctcagggagg
     5941 aggtggccgg gctacggcag gaaatgcagt ccatgagact gctggaacac aaaccggaga
     6001 cagcagagac cccggccgga acaccgcgcc ggtggtggcc gtggggaaaa cgctgacgcg
     6061 ctgtgcttgt ccaaccggcg acggccacgc aatgaattgc gtccccgtca ccggttatta
     6121 gagaacaata tggggattct ggcgctggcc gaccacggcc agaacccgga cttttctggt
     6181 ctggtcatca acatcataga ggacatgctg tcccatcaga ctgatccggc gaacgtcctc
     6241 gccatagcgt gagtccactt catagagtcg cgggaagtcg gccagtttgt ccgccgcagc
     6301 gagaacccgg tcggtaacgt tctgcgcatc ccacagggat gaattttccg cgatatagtc
     6361 aaacagcgtc gttacatcct cgatggctcc ctgcttccat tccacgtcat acggcatcgc
     6421 gcagttcctg ataacggcca tgcaggcgct ggcggagttg ttccgtggtc atacccggac
     6481 gcgtgtcagc acgggcggcg gtcactttgg cgcgaacctg atcttcatac tctgcctggg
     6541 ccagttcttc gttagccagt cgctgcaaca gcgcagcttt gtccgggcca ccttccggca
     6601 gtatgcgggg caggctttca gccagttccc gaagaaagtt ggcgttttcg tagtgtgtag
     6661 tgttgctcat tgcggagcct cccgggttaa tgcggcaata tgaaggaagt ttagcagggg
     6721 gcggtctgaa agaatatggt cgtgtcagtc aggctgatga agcagaaagt cggtaaaaaa
     6781 gggcttacgg catccatttt acgtcaaaac atatgccatg cagttgcgtg cataggcatg
     6841 cattaggata aaatttatcg ggcgcgtttc cggcagtttt ccgggggggt tgttgcctgt
     6901 tttatcccgt agccgccgga aacgccctga gcccgtctga gcggtgcgcg taatgacgcg
     6961 ttatggtaaa tccacttact gtctgccttc atagccatcg agataaacag ctgaaatggt
     7021 gtcagccagt agccttgatt ttttcggtgt gaggtctttg ctgcggtaat cggtacccgc
     7081 aaattgtgtt gtgccttgga gtgagtttaa aaattcctga ctgtgaaact gctgaatgcg
     7141 ttgctgcaca tcactccgtg attttccggt cgctttatcc tttgcgccca tatcccagaa
     7201 ctgccggaaa aaaggcacca tcattcgtgt tgtgtcagct ttctgggtgg aaaaattggg
     7261 atcaaaaccg tcatctgaag catatacgta atgccgggta tgctgttcca cgctgccctt
     7321 tggagcacag gcagtgagag ttgctgtcgc tgttatcatg gcgataagac acagtttgct
     7381 gccggaacgt ctcatgagcg tctccttacc tttaattctt ttttctcatt gagttatata
     7441 tccgcaggct atcttatcat cagtttctga ccgtgttcag tgaccgaaca acccgaacca
     7501 gacccccgtt tcttttcctg tcccggaggg ggctttctgg tatctcatat aatgatttca
     7561 gtaaaacata ctgaggccgg agaacggcgc cgtttgtgct ggtttttgtt gcatctggag
     7621 gggattgcgc atggtgtgag tggctgtttt tttgatcatt ttcacaaaaa cgcaaattta
     7681 cagacttgac atgaaaaaca aggcagcgta atttttatac tgtatataaa accagtggtt
     7741 atatgtacag tattttattt tagtttattg ttttaaaagt caaagaggaa aagtatatga
     7801 gtggcggaga cggacgaggt ccgggtaatt caggtctggg acacaatggt ggtcaggcca
     7861 gtgggaatgt gaacggtaca tcaggtaaag gtggcccttc atcaggtggt ggtacggatc
     7921 caaacagcgg gccgggctgg ggtacgacgc atacacctaa cggggatatt cataactata
     7981 acccggggga gtttggtaac ggcgggagta aacccggtgg taatggcggt aacagcggca
     8041 atcatagcgg gagctctggt ggcggacagt cttcggccac cgcgatggcc ttcggtctgc
     8101 ctgccctggc cactccgggg gctgaaggac tggctttatc cgtttccggc gatgcgttgt
     8161 cggccgctgt tgctgatgtg ctggctgccc tgaaaggtcc gtttaagttt ggtctgtggg
     8221 ggattgcgat ttacggagtg ctgccttctg agatagcaaa agatgatccg aaaatgatgt
     8281 caaaaatcgt gacgtcattg ccggcagaca cggtaacaga gacgccggtc agctccctgc
     8341 cgctggacca ggcgacggtc agcgtcacta aacgtgtggc ggatattgtg aaggacgagc
     8401 ggcagcatat tgcggttgtc accggccggc caatgagtgt tcctgtggtg gatgcgaaac
     8461 cgacaaaacg tccgggggta ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga
     8521 gcgtacctaa aggtgttccg gccgcgaaag ccccgccaaa aggcattatt gctgaaaaag
     8581 gtgattcacg tccggctggt tttacggccg gtggtaactc ccgtgaggcc gttattcgtt
     8641 tcccgaaaga gagcggacag aaaccggtct atgtgtcggt gacggatgtt cttactccgg
     8701 cacaggtaaa acagcgtctg gaggaagaaa agcgtcgcca gcaggcatgg gacgctgctc
     8761 acccggaaga ggggctgaaa agagagtatg ataaagcgaa agctgagctg gatgctgaag
     8821 ataaaaatat tgcgacctta aacagtcgca ttgcatcgac agagaaggcg atccccggtg
     8881 caagggctgc tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca aataaagatg
     8941 attttgtgac ttataacccg cctcatgaat atggctccgg gtggcaggat caggttcgct
     9001 atcttgataa ggatattcag aatcagaatg agaaattaaa agctgcccag acatctttaa
     9061 acgaaatgaa tgaatcctta tccagggata aggctgcgct ttccggggcg atggagagcc
     9121 ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa attaaatgag gaaaagaaaa
     9181 aacctcgtaa gggaactaaa gattacggcc atgattattt tcctgatccc aagactgaag
     9241 atattaaggg gttgggagag ttgaaagagg gtaaacctaa aacccctaaa caa
//
