LOCUS       NZ_OP242318             4072 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Escherichia coli strain LA207 plasmid pLAO91, complete sequence.
ACCESSION   NZ_OP242318
VERSION     NZ_OP242318.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363363
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028601025.1-RS_2024_03_24
            Annotation Date              :: 03/24/2024 01:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242318.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA207"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO91"
     gene            join(3139..4072,1..1214)
                     /gene="mobQ"
                     /locus_tag="PUP22_RS00005"
     CDS             join(3139..4072,1..1214)
                     /gene="mobQ"
                     /locus_tag="PUP22_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_079838644.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSSSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLKIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGESGLAALRSQL
                     LKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQHPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPVARRVDFTQGRLPPTLMPVM
                     SSVDRENRQLQLLLVMGVDDSLGGVVRLNGSLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADNHISPAPELMAFHLKTRETPLFAAVNTPEKQPDHLFRSLGFNHTWDEW
                     RREEHARTRVSGKKNDRDCSR"
                     /name="mobQ"
     gene            complement(1469..2176)
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="AINGBAKG_00002"
     CDS             complement(1469..2176)
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="AINGBAKG_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2706..2960)
                     /gene="mobC"
                     /locus_tag="PUP22_RS00015"
                     /old_locus_tag="AINGBAKG_00003"
     CDS             complement(2706..2960)
                     /gene="mobC"
                     /locus_tag="PUP22_RS00015"
                     /old_locus_tag="AINGBAKG_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2962..3138)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.315"
                     /coverage="100.0"
                     /evalue="5.65e-83"
     Typing          3139..3497
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.667"
                     /coverage="100.0"
                     /evalue="8.58e-169"
     Typing          complement(1470..2176)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.294"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..1211
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.892"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3053..4071
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.353"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aggaagcgat gagccatgct gccagcctga aaaaatcatc gccgttttcg tccggttttt
       61 cccgtattcc cctgatgcaa tgggctgcac cggaataccg tagtgagcag ggaaaaatcc
      121 agtctctgag ccagcaaatg gaaaaattgc gacagcatta ccggaaggtg gagaaacagg
      181 acattcccgc gagtcagacg gcatttgaaa aacagtggca gcagtggggg gaatccggcc
      241 ttgcagctct cagatcccag ttgcttaaac gtgaagagga attgcgccgg aaggagctgg
      301 aggatacaga acgtagtcgc gcggagcagc tcaggaaaaa tgacaatgcc gttatggatt
      361 caggggtgct gagagcagtc gttacgggat atggcgaggc tcccatgcct ggtaacgggg
      421 aaatgacctg ttaccttctg cttcacaacc ggaacggaga atatacgctc tgggggaacg
      481 agctggagaa ataccgcacc aggatctttg agagtgttga tttaatgcgt gacaggagtg
      541 gttatatctg tgaccgaagc gaaatcggtc aacatccacc actgcaacgt gtttattcct
      601 ctgcgacctt tgagcaactt ctgacgcagg tttgccagac atggccgcaa catacccgca
      661 atctccgcca gccgaagaca tggccggagt ctttctgtct tggagaagac cgccagccag
      721 ccatgccatc accggtggca aggagggtgg attttacgca gggacgtctg ccaccgacgc
      781 tgatgccggt aatgagttct gtcgaccggg aaaaccgaca acttcagctt ttgctggtca
      841 tgggcgtgga tgactctctg ggcggtgttg tccggcttaa tggttcgctg tatcccgcgt
      901 ttgctgtccc ctccgccgat aacaggcatc tggtcatcag tgcgctgacc gataaggggt
      961 tacgatatgc aggatatggt gttgccgtaa atcacgatgc agacaaccac atttcacctg
     1021 caccggaact tatggcgttt catcttaaaa cccgcgagac accgcttttt gctgccgtca
     1081 acacaccaga aaaacagccg gatcatctgt tccgttcact gggctttaat cacacctggg
     1141 atgaatggcg gcgcgaggag catgcacgaa cacgtgtctc cgggaagaag aatgatcgcg
     1201 actgttcacg gtaactgctg atagtatttt gttttgtttt acatcgtgtt atgttctctg
     1261 acattcatcg taactggatg aatgtcagat gcacacagat tgcacaaatc caactttatc
     1321 gttttcccgc ccccactaag ttgggggttc ttttttttga acgccataga atcgattatg
     1381 aagcaatttt atctcaccag tagtaccatc gcatacctct catggaaaaa acgcaacaga
     1441 gagcgtttta ggtgccttaa aatcgattct agatcgtcat cttgatctgt ttgtcttcct
     1501 tgaagcgaaa atgcagggct acaaccgtgc gccccttttt gatcgtctca accgttacag
     1561 ccaggtcgct ttttttgttc agttcgtcaa cacagggttt tattagatcc ctgtttaacg
     1621 ttttgaaatc tttatattta ccatcaagca tcagtgatga tttaaaatca tcgagggcga
     1681 taatacgttc cccagtactg cggaattgct ggagaatctc gtatattctt atgctgtaac
     1741 ttcttgataa attggagata tttttaataa caactctggt gaattgcccc ttcaactgtg
     1801 tcagataagg cattaccgcg tcagaaaaag tgatttgtgc tttcccctct cccctcgcgt
     1861 actgtgcccg atactgaatc cagcggaact cctcgcgctt ctcgtcgtcc ttgagaatga
     1921 tcgacctgtc ccacaaacga tcgatcgctt cctgtagctg aacctcggca tttttccgcc
     1981 ccatatcagg gaatgaatca aaatattccg cagctgtaat ggtcattgtc ttttgcaatt
     2041 caggtgaagc tacgtcagta ccggagttaa gtcggcttat gcagagcaat aagaaacgct
     2101 gttcttgtaa agttagcttg taactggcct caaccagcgc attggactta taggctatca
     2161 gagggtttga tgtcatttta accaccaggc caaagatgag gtgagataaa aacctatcac
     2221 ctttagcatt ggtgagcaat tatatttcca ccccccattt ttctgtgaat attaccttaa
     2281 atctcctcac ctttacctta aattacctca cccaagaacc ttaaattata tcacctttac
     2341 cttaaatttc cccacctata cacgtgtaac gcattgtttt taatcaggaa tttcgaccct
     2401 aaaacaagta ttaaaaacaa gtattaaaaa caagatctaa aaacttaacg gcgagcctgt
     2461 ggataacttg gagtgctgaa aataaaaacc caataaaaac aatgtgttgc aaaaaatcag
     2521 gattggagat tccgaatacc ccccctccaa ccctgtggaa atccagtatt catgcgggtt
     2581 cagcgcatat cacctgtcga aaaaccgtgc gtatgtgctc aacatggcga ctgattcact
     2641 tccgcttcct cgcccactcg ctcgctaacg ctcggtcgct gggctgcggc gagcggacga
     2701 tacaatcact gctctggcag cgcctccagc tcgttaatca gcggcagtat tcgcgtcaca
     2761 tcagcctgcc gtgtcacctc tgccttcagg aactggagta accaagctcg caactgcact
     2821 tcctccggct ttctgacctt tgccagcaat gctgcaccca caatgacctt ctgcgctgtg
     2881 tccaggctcc ggctcttcgc cttcaggcgc tgtaatctcg cctcagcatc agcaatcttc
     2941 tgttcgagcg ttctgctcat gatgtaactc catgcgcaat gaaaaatcgc attttagcgc
     3001 gtcactggta gtttaaaaac tgagctggca taatgcacgg caaatcacga agtgcgcact
     3061 tatacaattt ccactccgtt tcaattgtgt gcgccctgcg gggctgaaag aaaactgcaa
     3121 aaaggcatta cggcagaaat ggcaatttat catctcagta tgaaaatcat ctccagaagc
     3181 aacggctaca gcgccgttgc ttctgctgcc tatcgttcta gttcactaat gcttgatgag
     3241 cgtaccggac tgactcacga ctacacccgc aaaagcggtg tggctgaggc tgtgattctg
     3301 acccccgcga cagcccctgc ctggtgtacg aatcgtgccg aactctggaa tgccgttgag
     3361 aaggccgaac gccggaagaa ctcacaactt gcgcgggaaa ttgaactcgc catcccccac
     3421 gaactgccgc aggatgccgc acgggaaacc gttctcgcct ttgtcagaga aaactttgtc
     3481 agtcagggca tgattgctga tgtggcgttc catcacatgg ataaaacaaa tccccatgcg
     3541 cacatcatgc tgaccacgag ggctgtcggg cctgctgggt tcggcggaaa ggtcagggac
     3601 tggaacgacc ggacacatgc cgagacgtgg cgtgcatcat gggctgacca tgcaaacagg
     3661 gcactggcga acgccggtta tcaggaagag atagaccatc gttcatacga gcgacagggg
     3721 cttgagaaaa cgccgggtat tcacctcgga aagtctgcct gtgcgatgga aacacgcggt
     3781 atcgaaacag aacgcggcga acagaatcgt ctgattaaca ggcttaacct ggaaatacag
     3841 atttcccgca ccgaactcag gaacagcggg ctgacagagc ctgcccgccg tgtggctgac
     3901 cttctgaaga tcgggcttcc tgacaacgca acttcgtaca cactacgtga catcatcgaa
     3961 gcgttgccga aggacagtaa cgcggcatgg aaactcacca gtaacgccat gtcgatgatg
     4021 gcagatatgc aggcgacacg cagacgctgg gaagagctga acaccgaacg aa
//
