LOCUS       NZ_OP242317             4993 bp    DNA     circular CON 24-MAR-2024
DEFINITION  Escherichia coli strain LA207 plasmid pLAO90, complete sequence.
ACCESSION   NZ_OP242317
VERSION     NZ_OP242317.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29363363
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Plasmids from ESBL+ E. coli from PDR Laos
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Snaith,A.E., Dunn,S.J., Moran,R.A. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Institute of Microbiology and Infection,
            University of Birmingham, University of Birmingham, Birmingham B15
            2TT, United Kingdom
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.7
            Sequencing Technology        :: Oxford Nanopore
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_028601015.1-RS_2024_03_24
            Annotation Date              :: 03/24/2024 01:18:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OP242317.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LA207"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAO90"
     gene            complement(join(4830..4993,1..274))
                     /locus_tag="PUP22_RS00005"
     CDS             complement(join(4830..4993,1..274))
                     /locus_tag="PUP22_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046597188.1"
                     /translation="MKLKGFWLSAIVIAACNGMGFLFLYLSKTYFFPMVTFKDNLGNLG
                     SSLVTYSTAMMALLIAMVVIIFGLDGPRLHNFKKSGYLHVTYFIYMITFFELGLTMALS
                     LLCLSNISSIKVASYSLTFAFITFLLIGCLGIQLLNIKKNL"
                     /name="hypothetical protein"
     gene            complement(285..1118)
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="KELNKLDD_00001"
     CDS             complement(285..1118)
                     /locus_tag="PUP22_RS00010"
                     /old_locus_tag="KELNKLDD_00001"
                     /inference="COORDINATES: protein motif:HMM:NF027299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072775497.1"
                     /translation="MILLAGKKMKTNYFTHRLFPSDGAVICEPLKTSFDNMIATNRNTA
                     TLANEYHVIQHVSGDVYTFIKTNSRDVFRKLDTSSNICVDLSNILNQHEKIAFASFFIL
                     KGDVIGFSNTLYSPRVGKLAEIYDAAMYSRNGNHNINFEPITNIVTEQDVMNYAHVGKI
                     TMKIEKSQGIIGGLGTLFSGNVKYDDVDSFEIKIIPKRAKDIKDTFAGLMQARPQEVSS
                     VAVSAKEHIGDVATDLNVIMSNTVYDFVNPNDTTTIEVQMEKNYNNNTTLRSLGY"
                     /name="hypothetical protein"
     gene            complement(1402..1935)
                     /locus_tag="PUP22_RS00015"
                     /old_locus_tag="KELNKLDD_00002"
     CDS             complement(1402..1935)
                     /locus_tag="PUP22_RS00015"
                     /old_locus_tag="KELNKLDD_00002"
                     /inference="COORDINATES: protein motif:HMM:NF025323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafO family toxin"
                     /protein_id="WP_072775495.1"
                     /translation="MPPHLPRIIVHIHDDLSRLPGVESVAREFETYKSYAFQIATDSEC
                     DVSNFLPTLDEEDQKAFRPDHPFFGRDRLFYGQRIRAANDPVFHVHLHHQETIKSWYDF
                     DKADVFVSQWDCVSDAALVYAYLKTESDDYHFLLLEIVAPGAHDLYQTEGKIAEWRHKT
                     RNFRYSSQNPSLRT"
                     /name="type II toxin-antitoxin system YafO family toxin"
     gene            complement(1986..2144)
                     /locus_tag="PUP22_RS00020"
                     /old_locus_tag="KELNKLDD_00003"
     CDS             complement(1986..2144)
                     /locus_tag="PUP22_RS00020"
                     /old_locus_tag="KELNKLDD_00003"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_213131687.1"
                     /translation="MSGMRKASEHRSPIKPAMSYALFQDFINAGLAAQKSDEAKSAVKN
                     HRITNQN"
                     /name="hypothetical protein"
     gene            2364..2954
                     /locus_tag="PUP22_RS00025"
                     /old_locus_tag="KELNKLDD_00004"
     CDS             2364..2954
                     /locus_tag="PUP22_RS00025"
                     /old_locus_tag="KELNKLDD_00004"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_072775496.1"
                     /translation="MSTTKEKEKTDFPEALLSIWGRTILGSGWTTVPNELLKNQSHLGI
                     SNSELVLLIHLISFMHSASAKIFPSIDSLAERMNQDRRTIQRTIQRLEDKELIRKRVRS
                     TGKTDVGMSNVYDISPLMLKLINIQLPTMSPPPNTHTCPKCKKFAKSESDIERLFGYRR
                     ISAEKMVIQSWCKECRGAPKKPQSPKEGSPDLP"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3694..3843)
                     /locus_tag="PUP22_RS00030"
                     /old_locus_tag="KELNKLDD_00005"
     CDS             complement(3694..3843)
                     /locus_tag="PUP22_RS00030"
                     /old_locus_tag="KELNKLDD_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          complement(3837..4051)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.908"
                     /coverage="100.0"
                     /evalue="7.28e-73"
     Typing          4027..4513
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.081"
                     /coverage="93.0"
                     /evalue="5.84e-177"
ORIGIN
        1 tcatataaat aaaatatgta acatggagat agccgctttt tttaaagtta tgcaagcgag
       61 gtccatccaa gccaaagatt ataacaacca tagcaataag caacgccatc atcgctgtcg
      121 aataagtaac caaggaagaa ccaaggttac ctaaattatc cttaaacgtt accatcggga
      181 agaaataggt ttttgaaagg tatagaaata aaaagcccat gccattacat gcagctataa
      241 ctatagcgct caaccaaaaa cccttaagct tcacaatgca atcctcagta acctaaactt
      301 cttaatgttg tattgttatt ataatttttt tccatctgaa cctcgatagt agtagtgtca
      361 tttggattta caaaatcata tacggtatta gacattataa cattcaaatc tgttgcaacg
      421 tcaccaatgt gttctttagc tgacacagcg accgagctaa cctcttgagg cctagcctgc
      481 atcaaacctg caaaggtatc ctttatgtct ttagcccgtt tagggattat ctttatctca
      541 aacgaatcca catcgtcata ctttacgttc ccagagaaaa gcgtaccaag cccaccaata
      601 atgccttgag acttctctat tttcatcgtt attttaccaa catgggcata attcataaca
      661 tcttgctcgg taactatgtt tgttatcggc tcaaaattta tgttgtggtt cccatttcgg
      721 gaatacattg cagcatcata tatttctgcc aacttcccaa cacgaggaga gtacaacgta
      781 ttactaaacc ctataacatc acccttcaat atgaaaaaag atgcaaaggc tattttttca
      841 tgttgattta agatgtttga gagatctaca catatattac tactcgtatc tagttttcta
      901 aaaacgtctc tgctatttgt ttttataaac gtatatacat cgccagacac atgctgaatt
      961 acatgatact cattggctag cgttgcagtg tttctgttag tagcgatcat attatcaaaa
     1021 ctagttttta atggctcaca aataacagcg ccatctgaag gaaacaatct gtgtgtgaag
     1081 taatttgttt tcattttttt cccagctagt aaaatcatag aaacgcatta tattttcttc
     1141 tttttatatt ctatttctta tggaagaaca atgcttcttt aaaattccaa gttttgcata
     1201 ttaaatgatc tgtcaccgaa ggtcgtaaag ctaggccacc aactctattt accataacat
     1261 ccgttatcgg aaggtagctc cccccgagcg cattcttgtt ccgaccctgc cgcttaccaa
     1321 atacctgtcc cgccgttttc ccttcgggaa gcgtgaaagc acaaaggccc gtatttacgg
     1381 gcctttgtgc tttaattgga ttcaagttct cagagaagga ttctggctgc tatagcggaa
     1441 atttctagtt ttatgtctcc actctgcaat tttaccttct gtttgataaa ggtcatgagc
     1501 ccctggagcc acgatctcta acaacaaaaa gtggtaatca tcactttctg ttttcaaata
     1561 tgcatagacc aatgcagcgt cacttacaca atcccactga ctaacaaaaa catctgcttt
     1621 atcaaaatca taccaagatt taatagtttc ttggtgatgc aagtgaacgt gaaagacagg
     1681 atcgtttgct gcccttatcc tttggccata gaaaagacga tctcgaccaa aaaacgggtg
     1741 gtcgggacga aatgcctttt gatcttcttc atccaatgta ggaaggaagt tcgaaacatc
     1801 acattcacta tctgtcgcta tctgaaacgc ataggattta tatgtttcaa attccctagc
     1861 cacggactca actccaggta gcctagataa gtcatcatga atatgaacaa tgattcgagg
     1921 aaggtgcggc ggcatccacg tactcccgag caaaatagga gtacgaatga tatcacagac
     1981 aaatatcagt tctgattggt aatccgatga tttttaacag cagacttagc ctcatcgctc
     2041 ttctgagctg ccagccccgc attgataaaa tcttggaaca gagcgtaact catggcgggt
     2101 tttatcggag aacgatgttc cgatgctttt ctcatcccag acatgtgaat acctcgctta
     2161 atgacgtagt aggaatagca aactattcct cttcgaataa tgttaagagc aagcgtcgtg
     2221 acatgtcacg aaaaggcgtg cctttaaggt aaaattaggg tggcgttaac gccaccctaa
     2281 taataactac tcttacatac actatgatat agtgtatgta agagtagtta tgtcatgata
     2341 aatgattcaa aaaagtaacc gctatgagta caactaaaga gaaagaaaag actgatttcc
     2401 ctgaagcctt actatccata tgggggagga caatccttgg ttcaggatgg acaaccgtcc
     2461 ccaacgaact gctaaaaaat caatctcact tgggtatcag taactcagag ttagttctcc
     2521 ttatccattt gatatcattt atgcatagtg cttctgcaaa aatattccca tcaatagatt
     2581 ctctagcaga aagaatgaac caagaccggc gtaccattca gagaaccata caaaggctcg
     2641 aagataagga gctgataaga aaaagagtac gttcaactgg gaaaaccgat gtagggatga
     2701 gtaatgtgta tgatatttct ccattaatgc tcaagcttat caacatacag ctaccaacaa
     2761 tgagccctcc tccaaacaca catacatgtc caaaatgtaa aaaatttgca aaatccgaat
     2821 ctgacatcga acgattattt ggttaccgaa ggatttcggc agaaaagatg gtcatacaaa
     2881 gctggtgtaa agaatgcaga ggtgctccaa aaaaaccgca aagtcccaaa gaaggctcgc
     2941 ctgatttacc ataacatctg ttgcgtgcac cgccgcagag gccctcagcg cgtttctgac
     3001 gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct
     3061 gtttttacgg catgcatgag tatgcgcaca agtgcatagc gcggaaattc acaccattca
     3121 gccggggtaa gtcctgtttc cggcgttctt cccatccgaa aacggtgaac ggggacgcaa
     3181 tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aacccgctgt cagcacgttc
     3241 aggaaggggg tgatgtacaa atggcacaga ggatgaaagt gacgtatgag ctgaaggaat
     3301 gatgtaaaag tgatgtataa gtgatgtata agtgatgtaa aagtgatgta aaagtgatgt
     3361 aattttgatg tacttttgat gtaattctgt agtcaggaag aataacccgt tcatgcaggc
     3421 atgaacgata cagtgaggaa cgcttttttg tcacgatctc cccttaacct gcgtaaatca
     3481 cccgccatgg ttgattattt ttcaacctca aaatggtcat acacctaacg cagccgcgat
     3541 tttcatcgca actgcatgaa ttaaaagaaa aaaaaggaat atttcgtttt cacgagtaat
     3601 acccgggtat taaaaatcca ctgtaattac atgaaaataa agaaataaaa ggtaataccg
     3661 gagtattacc acgtacgcgg gcaagcgaaa aagctataac gcttcccggc cgacaggcga
     3721 cccaaaatcc acaggggcat gcaggttctc ttcagtaacc tgcgccatta aatcaaaaag
     3781 gtcatattcg ccgggaagct gcgtatcagt cgaatccagt tgttcatcat attcagatgg
     3841 catggggtga tatccagtag ctgagaggtt gttagcgggt ttcggggcgc agccctgaac
     3901 cagtcacgta gcgctagcgg agtgtataca ggctgaatat gttaggtcgg agtggtaatt
     3961 cagggaagtt attcatgttg caagggaaaa tgcggctata ggacgtaagt gcaaaatgca
     4021 ggtaacggtg acatgacgcc tcctcgctca ctcggtcgct acgctcctgc cgtgagactg
     4081 cggcgggcgt tacctgctca cgaatgacaa gataaaaaga tggctctcaa gtcaggaaag
     4141 gccggaaaaa ggatacggcg ctgccgtttt tccataggct ccgcccccct gacaagcatc
     4201 acaaaatctg acgctcaaat cagtggcggc gaaacccgac aggactataa agatcccagg
     4261 cgtttccccc tggtagctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     4321 tcattccgct gttatggccg cgtttatctc attccacgcc tgacactcag ttccgggtag
     4381 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ctactgcgcc
     4441 tttccggtaa ctgtcttctt gagtccaacc cggaaagaca cgacaacgcg ccattggcag
     4501 cagccattgg taagaggatt cacggagcag agtagtagag tatttctatg gagttcttga
     4561 agtggtggcc tgactacggc tacactaaaa ggacagtttt ggtatctgcg ctccgtgaaa
     4621 gccagttacc ttcgggagaa agagttggta gctctcactt ccggcaaaca aaccaccgtt
     4681 ggtagcggtg gtttttttgt ttacagagca agagactacg ctgcactctc aaaaatctca
     4741 agacgatcct tacacattct gcattttatc aactcaccaa aaatggtcac aaaccgtttc
     4801 acctgtgatg aacatcacag tttggttgat taaagattct tttttatatt taagagttga
     4861 atacctaaac atcctatcaa caaaaaagta ataaatgcaa aagtcaatga ataagaagcc
     4921 accttaatac ttgagatatt ggacaaacaa agtaacgaaa gagccatggt taagccgagc
     4981 tcgaaaaacg tta
//
